Anti-complement Activity of Triterpenoids from the Whole Plant of Patrinia saniculaefolia

Ren-Bo An,나민균,이형규,배기환,민병선 한국생약학회 2008 Natural Product Sciences Vol.14 No.4

Two oleanane-type triterpenes (1, 2) and their glycosides (4 - 6), and one ursane-type triterpene (3) have been isolated from a methanolic extract of Patrinia saniculaefolia Hemsley (Valerianaceae) through repeated silica gel and reversed-phase C-18 column chromatography. Their chemical structures were determined as oleanolic acid (1), oleanonic acid (2), 23-hydroxyursolic acid (3), 3-O-α-L-arabinopyranosyl-oleanolic acid (4), 3- O-β-D-glucopyranosyl-oleanolic acid (5), and oleanolic acid 3-O-[α-D-xylopyranosyl-(1→ 3)-β-D-glucuronopyranoside- 6-O-butyl-ester] (6) on the basis of their MS, ¹H-, and 13C-NMR spectral data. All compounds were isolated from the whole plant of the P. saniculaefolia for the first time. These compounds were examined for their anti-complement activity against the classical pathway of the complement system. Among them, compounds 1 - 3 exhibited anti-complement activity with IC50 values of 470.1, 212.2, and 121.0 μM, respectively, whereas compounds 4 - 6 were inactive. These results suggest that the carbonyl or hydroxy group at C-3 in the oleananeand/ or ursane-triterpenes are important for the anti-complement activity against the classical pathway.

In vitro Free Radical Scavenging and Hepatoprotective Compound from Sanguisorbae Radix

Ren-Bo An,Yu-Hua Tian,Hyuncheol Oh,Youn-Chul Kim 한국생약학회 2005 Natural Product Sciences Vol.11 No.3

In the course of searching for hepatoprotective agents from natural products, four compounds wereisolated from the MeOH extract of Sanguisorbae Radix, as guided by their DPPH free radical scavenging activity.The structures were determined as 4,5-dimethoxy-3-hydroxybenzoic acid methyl ester (1), (+)-gallocatechin (2),methyl 6-O-galloyl-β-D-glucopyranoside (3), and pomolic acid 3-O-[α O-[β-D-gluco-pyranosyl] ester (ziyu-glycoside I) (4). Compounds 2 and 3 showed significant DPPH fre radical scavengingefects, exhibiting IC50 values of 1.4 and 13.0M, respectively. L-Ascorbic acid was used as a positive controland exhibited the IC50 value of 50.3M. In evaluation of the hepatoprotective activity of the isolated compoundson drug-induced cytotoxicity, compound 2 50 value of91.84 ± 11.0M on tacrine-induced cytotoxicity in Hep G2 cells, while silybin, a positive control, exhibited EC50value of 122.4 ± 12.5M.

Coumarins and Chromones from Angelica genuflexa

Ren Bo An,Bo-Young Park,Jung-Hee Kim,Ok-Kyoung Kwon,Joongku Lee,Byung-Sun Min,Kyung-Seop Ahn,Sei-Ryang Oh,Hyeong-Kyu Lee 한국생약학회 2005 Natural Product Sciences Vol.11 No.2

Thirteen compounds were isolated from the roots of Angelica genuflexa through repeated silica gelcolumn chromatography. Nine coumarins, isoimperatorin (1), osthol (2), demethylsuberosin (3), oxypeucedanin(4), heraclenin (5), pabulenol (7), umbelliferone (8), oxypeucedanin hydrate (9) and marmesinin (11), and fourchromones, hamaudol (6 10), sec-O-glucosylhamaudol (12) and prim-O-glucosylcimifugin (13), wereidentified by physicochemical and spectroscopic analysis. Among these, compounds 3, 5, 6, 8, 12, and 13 wereisolated for the first time from the roots of Angelica genuflexa. These coumarins and chromones were examinedfor their anticomplement activity. Demethylsuberosin (3) showed a weak anticomplement activity with an IC50value of 390M.

Anti-Inflammatory Activity of Compounds from the Whole Plant of Patrinia saniculaefolia

Ren Bo An,나민균,민병선,장현욱,배기환 한국생약학회 2011 Natural Product Sciences Vol.17 No.2

An in vitro bioassay-guide revealed that the methanol (MeOH) extract of the whole plant of Patrinia saniculaefolia (Valerianaceae) showed cyclooxygenase-2 (COX-2) and 5-lipoxygenase (5-LOX) dual inhibitory activity by assessing their effects on the production of prostaglandin D2 (PGD2) and leukotriene C4 (LTC4) in mouse bone marrow-derived mast cells (BMMCs). Phytochemical study of the MeOH extract of this plant led to the isolation of twelve compounds; b-farnesene (1), squalene (2), nardostachin (3), patridoid I (4), patridoid II (5), patridoid II-A (6), oleanolic acid (7), oleanonic acid (8), 23-hydroxyursolic acid (9), oleanolic acid 3-O-a-L-arabinopyranoside (10), oleanolic acid 3-O-b-D-glucopyranoside (11), oleanolic acid 3-O-[b-D-xylopyranosyl-(1 → 3)-b-D-(6-O-butyl)glucuronopyranoside] (12). Among the compounds, 4 and 5 strongly inhibited both the COX-2-dependent PGD2 generation with IC50 values of 8.7 and 13.6 mM, respectively, and the generation of LTC4 in the 5-LOX dependent phase with IC50 values of 41.7 and 46.9 mM, respectively, which suggest that the anti-inflammatory activity of P. saniculaefolia might occur in part via the inhibition of both PGD2 and LTC4 generation by 4 and 5.

現代漢語形容詞 輕易 容易 隨便 的比較硏究

REN HAI BO 한국중국언어학회 2007 중국언어연구 Vol.0 No.24

본고는 현대한어 중“轻易”와 관련된 단어 “容易”和“随便”의 통사 및 의미관계를 연구하고자 한다. “轻易”는 종종 부사로 오인되기 쉬운데, 그것은“轻易”가 문장에서 대부분 통사성분이 될 수 있는것 외에도 부사어로 충당되어 사용되는 빈도가 매우 높기 때문이다. “容易”和“随便”은 모두 형용사이기는 하지만 이들이 문장에서 부사어로 충당되는 것 또한 이들의 주요한 문법기능이다. 따라서 부사어로서 이 세 단어를 혼용하여 사용하는 것은 언어를 학습하는 중에 있어 실질적인 문제가 될 수 있다. 본고는 다량의 언어자료 기초 위에 이 세 단어가 문장에서 각각 놓여져 있는 통사적 특징 분석과 이들 간의 상호비교 분석을 통해 이들이 문장에서 쓰이는 기본적인 의미가 나타내는 기능을 파악하고자 한다. “轻易”가 문장에서 부사어로서 나타내는 기본적인 세 가지 기능은 다음과 같다: 1. 주관적인 태도를 나타낸다. 2. 객관적인 상태를 나타낸다. 3. 앞의 두 가지 상태를 융합한다. “容易”구의 의미가 나타내는 주요 기능은“轻易”구의 두 번째 의미가 나타내는 기능과 서로 연관되어 있고,“随便”구의 의미가 나타내는 기능은 주로“轻易”구의 첫번째 의미가 나타내는 기능과 서로 연관되어 있다. “轻易”구에서 첫번째 의미가 나타내는 기능은 절대적인 우세를 차지한다. 따라서, “轻易”와“随便”을 호환하는 것은 상대적으로 쉽지만“轻易”와“容易”를 호환하는 것은 상대적으로 어려움이 있다. 의미상의 표현 기능이 일치되는 전제하에서 단어의 호환은 여전히 통사조건의 제약을 받는다.

CTE Method and Interaction Solutions for the Kadomtsev-Petviashvili Equation

Bo Ren 한국물리학회 2017 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.70 No.4

The consistent tanh expansion method is applied to the Kadomtsev-Petviashvili equation. The interaction solutions among one soliton and other types of solitary waves, such as multiple resonant soliton solutions and cnoidal waves, are explicitly given. Some special concrete interaction solutions are discussed both in analytical and graphical ways.

Inverse Force Estimation in a New Lab Simulation Approach for Vibration Prediction of Building-Mounted Small Wind Turbine

Bo Ren 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.5

The structural vibration generated by building mounted small wind turbine was fully investigated by author. In order to achieve this, the real vibration condition of wind turbine in field was accurately simulated in Lab for research. There are some difficulty during the whole simulation process for further quantification of vibration levels. One of the main concerns was the correct estimation of the excitation or contact force at the interface between vibration source (turbine) and receiver (building or walls). However, the relevant simulation method in Lab is not available so far. In this paper, the direct measurement of force was investigated and found unsuitable. A new simulation method was proposed including solving the problem of power supply (DC electric motor used), automatic control of rotational speed of motor and indirect interface force determination. At last, the new established finite element models (FEM) was validated by experimental data with a good initial agreement.

Synthesis and Antibacterial Activity of 1,3-Diallyltrisulfane Derivatives

Ren, Fang-Kui,He, Xiao-Yan,Deng, Li,Li, Bo-Heng,Shin, Dong-Soo,Li, Zhu-Bo Korean Chemical Society 2009 Bulletin of the Korean Chemical Society Vol.30 No.3

A series of novel 1,3-diallyltrisulfane analogues were synthesized and assayed in vitro for antimicrobial activity against Gram positive, Gram negative bacteria and fungi. The antimicrobial activity of the 1,3-diallyltrisulfane derivatives showed, on the whole, very potent towards all the tested Gram positive, Gram negative and fungi (MIC ranging from 4 to 256 μg/mL). 1,3-Di(pent-4-enyl)trisulfane 3b and 1,3-bis(3-methylbut-2-enyl)trisulfane 3e exhibited the strongest antibacterial activity among all the compounds, and both of them were more active than 1,3-diallyltrisulfane (DATS). Results indicated the relationship of either carbon number or lipophilicity with antimicrobial activity presented “V” shape. These observations provided some predictions in order to further design 1,3-diallyltrisulfane derivatives with antimicrobial activity.

Chromone Glycosides and Hepatoprotective Constituents of Hypericum erectum

Ren Bo An,Jin-Seon Beom,손동환,Youn Chul Kim,Gil Saeng Jeong 대한약학회 2009 Archives of Pharmacal Research Vol.32 No.10

Two chromone glycosides, hyperimone A [7-(β-D-glucopyranosyloxy)-5-hydroxy-2-(1-methylethyl)-4H-1-benzopyran-4-one (1)] and hyperimone B [7-(β-D-glucopyranosyloxy)-5-hydroxy-3-methyl-4H-1-benzopyran-4-one (2)], together with six known compounds were isolated from the methanolic extract of the whole plant of Hypericum erectum. 1,3,5,6-Tetrahydroxyxanthone (5) and I3, II8-biapigenin (6) showed moderate hepatoprotective activity with EC50 values of 160.2 ± 0.6 μM and 217.7 ± 1.3 μM, respectively, against tacrine-induced cytotoxicity in HepG2 cells.

Free Radical Scavenging and Hepatoprotective Constituents from the Leaves of Juglans sinensis

Ren-Bo An,Hyun-Chul Kim,Yu-Hua Tian,Youn-Chul Kim 대한약학회 2005 Archives of Pharmacal Research Vol.28 No.5

In the course of searching for hepatoprotective agents from natural products, six compounds were isolated from the MeOH extract of the leaves of Juglans sinensis, as guided by their DPPH free radical scavenging activity. The structures were determined as juglanoside B (1), quercetin 3-O-α-L-arabinofuranoside (avicularin, 2), quercetin 3-O-α-L-arabinopyranoside (guaijaverin, 3), quercetin 3-O-α-L-rhamnopyranoside (quercitrin, 4), (+)-catechin (5) and quercetin 3-O-β-D-galactopyranoside (hyperin, 6). Compounds 2-6 showed significant DPPH free radical scavenging effects. An evaluation for the hepatoprotective activity of the isolated compounds on drug-induced cytotoxicity was conducted, and compounds 1, 2, and 5 showed protective effects against nitrofurantoin-induced cytotoxicity, and compound 5 also exhibited a moderate protective effect on amiodarone-induced cytotoxicity in Hep G2 cells.