http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
CK2 phosphorylates AP-2α and increases its transcriptional activity
( Kai Qun Ren ),( Shuang Lin Xiang ),( Fang Li He ),( Wen Feng Zhang ),( Xiao Feng Ding ),( Yan Yang Wu ),( Li Ping Yang ),( Jian Lin Zhou ),( Xiang Gao ),( Jian Zhang ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.7
Transcription factor AP-2α involves in the process of mammalian embryonic development and tumorigenesis. Many studies have shown that AP-2α functions in association with other interacting proteins. In a two-hybrid screening, the regulatory subunit β of protein casein kinase 2 (CK2β) was identified as an interacting protein of AP-2α; we confirmed this interaction using in-vitro GST pull-down and in-vivo co-immunoprecipitation assays; in an endogenous co-immunoprecipitation experiment, we further found the catalytic subunit α of protein casein kinase 2 (CK2α) also exists in the complex. Phosphorylation analysis revealed that AP-2α was phosphorylated by CK2 kinase majorly at the site of Ser429, and such phosphorylation could be blocked by CK2 specific inhibitor 4,5,6,7-tetrabromobenzotriazole (TBB) in a dose-dependent manner. Luciferase assays demonstrated that both CK2α and CK2β enhanced the transcription activity of AP-2α; moreover, CK2β increased the stability of AP-2α. Our data suggest a novel cellular function of CK-2 as a transcriptional co-activator of AP-2α.
Expression and Functional Role of ALDH1 in Cervical Carcinoma Cells
Rao, Qun-Xian,Yao, Ting-Ting,Zhang, Bing-Zhong,Lin, Rong-Chun,Chen, Zhi-Liao,Zhou, Hui,Wang, Li-Juan,Lu, Huai-Wu,Chen, Qin,Di, Na,Lin, Zhong-Qiu Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4
Tumor formation and growth is dictated by a very small number of tumor cells, called cancer stem cells, which are capable of self-renewal. The genesis of cancer stem cells and their resistance to conventional chemotherapy and radiotherapy via mechanisms such as multidrug resistance, quiescence, enhanced DNA repair abilities and anti-apoptotic mechanisms, make it imperative to develop methods to identify and use these cells as diagnostic or therapeutic targets. Aldehyde dehydrogenase 1 (ALDH1) is used as a cancer stem cell marker. In this study, we evaluated ALDH1 expression in CaSki, HeLa and SiHa cervical cancer cells using the Aldefluor method to isolate ALDH1-positive cells. We showed that higher ALDH1 expression correlated with significantly higher rates of cell proliferation, microsphere formation and migration. We also could demonstrate that SiHa-ALDH1-positive cells were significantly more tumorigenic compared to SiHa-ALDH1-negative cells. Similarly, SiHa cells overexpressing ALDH1 were significantly more tumorigenic and showed higher rates of cell proliferation and migration compared to SiHa cells where ALDH1 expression was knocked down using a lentivirus vector. Our data suggested that ALDH1 is a marker of cervical cancer stem cells and expand our understanding of its functional role.
Han, Yu-Lin,Yin, Hai-Yang,Xiao, Er-Tian,Sun, Zhi-Lin,Li, Ai-Qun Techno-Press 2006 Structural Engineering and Mechanics, An Int'l Jou Vol.22 No.2
NiTi-wire shape memory alloy (SMA) dampers, that utilize NiTi SMA wires to simultaneously damp tension, compression and torsion, was developed for structural control implementation in this study. First, eight reduced-scale NiTi-wire SMA dampers were constructed. Then tension, compression and torsion experiments using the eight reduced-scale NiTi-wire SMA dampers of different specification were done. The experimental results revealed all of the eight reduced-scale NiTi-wire SMA dampers had the ability to simultaneously supply tension-compression damping and torsion damping. Finally, mechanics analysis of the NiTi-wire SMA dampers was done based on a model of the SMA-wire restoring force and on tension-compression and torsion damping analysis. The damping analytical results were found to be similar to the damping experimental results.
Lin, Ligen,Lee, Jong Han,Wang, Ruitao,Wang, Ru,Sheikh‐,Hamad, David,Zang, Qun S.,Sun, Yuxiang MDPI 2018 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.19 No.10
<P>Ghrelin via its receptor, the growth hormone secretagogue receptor (GHS-R), increases food intake and adiposity. The tissue-specific functions of GHS-R in peripheral tissues are mostly unknown. We previously reported that while GHS-R expression is very low in white and brown fat of young mice, expression increases during aging. To investigate whether GHS-R has cell-autonomous effects in adipose tissues, we generated <I>aP2</I>-Cre-mediated GHS-R knockdown mice (<I>aP2</I>-Cre/<I>Ghsr<SUP>f/f</SUP></I>). We studied young (5–6 months) and old (15–17 months) <I>aP2</I>-Cre/<I>Ghsr<SUP>f/f</SUP></I> mice and their age-matched controls. Interestingly, young <I>aP2</I>-Cre/<I>Ghsr<SUP>f/f</SUP></I> mice had normal body weight but reduced fat; old mice showed pronounced reductions of both body weight and body fat. Calorimetry analysis revealed that <I>aP2</I>-Cre/<I>Ghsr<SUP>f/f</SUP></I> mice had normal food intake and locomotor activity at both young and old age; but intriguingly, while energy expenditure was normal at young age, it was significantly increased at old age. Both young and old <I>aP2</I>-Cre/<I>Ghsr<SUP>f/f</SUP></I> mice exhibited improved insulin sensitivity and glucose tolerance. Importantly, old <I>aP2</I>-Cre/<I>Ghsr<SUP>f/f</SUP></I> mice maintained higher core body temperature at 4 °C, and showed higher expression of the thermogenic uncoupling protein 1 (<I>UCP1</I>) gene. The ex vivo studies further demonstrated that GHS-R deficient white adipocytes from old mice exhibit increased glucose uptake and lipolysis, promoting lipid mobilization. Despite the fact that the in vivo phenotypes of <I>aP2</I>-Cre/<I>Ghsr<SUP>f/f</SUP></I> mice may not be exclusively determined by GHS-R knockdown in adipose tissues, our data support that GHS-R has cell-autonomous effects in adipocytes. The anabolic effect of GHS-R in adipocytes is more pronounced in aging, which likely contributes to age-associated obesity and insulin resistance.</P>
Lin, Zhen-Jian,Lu, Xiao-Ming,Zhu, Tian-Jiao,Fang, Yu-Chun,Gu, Qian-Qun,Zhu, Weiming 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.9
An endophytic Streptomyces sp. (AC-2) was isolated from the root of Cistanches deserticola Y.C.Ma.. Chemical investigations of the culture broth of AC-2 afforded fifteen compounds including K1115 A (1), tyrosol (2), phenylethylamine derivatives (3, 4), cyclic dipeptides (5-8), nucleosides and their aglycones (9-13), N-acetyltryptamine (14), and pyrrole-2-carboxylic acid (15). Only tyrosol can promote an increase of intracellular cAMP special on GPR12 transfected cells, such as CHO and HEK293, which means it may be a possible ligand for GPR12.