A review on Silver-mediated DNA base pairs: methodology and application

Qiao Sun,Xiao Xie,Yujie Song,Litao Sun 한국생체재료학회 2022 생체재료학회지 Vol.26 No.2

The investigation of the interaction between metal ions and DNA has always attracted much attention in the fields of bioinorganic chemistry, supramolecular coordination hemistry, and DNA nanotechnology. Its mode of action can be simply divided into two aspects. On the one hand, it is non-specific electrostatic adsorption, mainly including Na+, K+, Mg2+, Ca2+ and other physiologically regulating ions; on the other hand, it is specific covalent binding, such as Pt2+, Hg2+, Ag+ and other heavy metal ions. This article focuses on the mechanism of action between Ag+ and DNA mismatch pair C-C, and summarizes its main characterization methods and various applications. It aims to provide a certain reference for the field of biological devices. With the development of cryoelectron microscopy and liquidcell TEM, the structure of C-Ag+-C is expected to be further characterized, which will be more widely used.

STABILITY OF MULTI–STAGE ACTIVE VIBRATION ISOLATION

Qiao Sun 한국소음진동공학회 2008 한국소음진동공학회 국제학술대회논문집 Vol.2008 No.7

An Efficient PEG/CaCl2-Mediated Transformation Approach for the Medicinal Fungus Wolfiporia cocos

( Qiao Sun ),( Wei Wei ),( Juan Zhao ),( Jia Song ),( Fang Peng ),( Shao Peng Zhang ),( Yong Lian Zheng ),( Ping Chen ),( Wen Jun Zhu ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.9

Sclerotia of Wolfiporia cocos are of medicinal and culinary value. The genes and molecular mechanisms involved in W. cocos sclerotial formation are poorly investigated because of the lack of a suitable and reproducible transformation system for W. cocos. In this study, a PEG/ CaCl2-mediated genetic transformation system for W. cocos was developed. The promoter Pgpd from Ganoderma lucidum effectively drove expression of the hygromycin B phosphotransferase gene in W. cocos, and approximately 30 transformants were obtained per 10 μg DNA when the protoplast suspension density was 106 protoplasts/ml. However, no transformants were obtained under the regulation of the PtrpC promoter from Aspergillus nidulans.

A Theoretical Study on the Mechanism of Decarboxylations for Hydroxymandelate Synthase

Qing-An Qiao,Qiuxian Li,Changchun Liu,Xiao Sun,Honglan Cai,Lixiang Sun,Huayang Wang 대한화학회 2017 Bulletin of the Korean Chemical Society Vol.38 No.6

Hydroxymandelate synthase is one type of alpha-keto acid-dependent oxygenases and plays an important role in the biosynthesis of hydroxyphenyl-pyruvate. The ferrous and ferryl intermediates in the reaction were considered to be very important in the DNA repair process. The mechanism of hydroxymandelate synthase was investigated by a theoretical method based on B3LYP/LACVP*. The data indicated that each decarboxylation went through a process of direct dioxygen insertion. The first oxidative decarboxylation experienced only one transition state, while there were two potential pathways for the second decarboxylation process. The calculation data showed that the stepwise one was favored to the concerted one due to the lower energy barrier. The process to form the iron-superoxide was the rate-limiting step. After two consecutive decarboxylations, a proton transfer reaction was needed to obtain the target products. Two possible transition states were found out to achieve this step. The one with a six-membered ring structure was preferred because of the lower activation energy. In addition, several ferryl species with high spins were captured in the whole process, which could be supplied as requisite substrates for DNA repair reaction.

Identification of Plasma Biomarkers in Drug-Naïve Schizophrenia Using Targeted Metabolomics

Qiao Su,Fuyou Bi,Shu Yang,Huiming Yan,Xiaoxiao Sun,Jiayue Wang,Yuying Qiu,Meijuan Li,Shen Li,Jie Li 대한신경정신의학회 2023 PSYCHIATRY INVESTIGATION Vol.20 No.9

Objective Schizophrenia (SCZ) is a severe psychiatric disorder with unknown etiology and lacking specific biomarkers. Herein, we aimed to explore plasma biomarkers relevant to SCZ using targeted metabolomics. Methods Sixty drug-naïve SCZ patients and 36 healthy controls were recruited. Psychotic symptoms were assessed using the Positive and Negative Syndrome Scale. We analyzed the levels of 271 metabolites in plasma samples from all subjects using targeted metabolomics, and identified metabolites that differed significantly between the two groups. Then we evaluated the diagnostic power of the metabolites based on receiver operating characteristic curves, and explored metabolites associated with the psychotic symptoms in SCZ patients. Results Twenty-six metabolites showed significant differences between SCZ patients and healthy controls. Among them, 12 metabolites were phosphatidylcholines and cortisol, ceramide (d18:1/22:0), acetylcarnitine, and γ-aminobutyric acid, which could significantly distinguish SCZ from healthy controls with the area under the curve (AUC) above 0.7. Further, a panel consisting of the above 4 metabolites had an excellent performance with an AUC of 0.867. In SCZ patients, phosphatidylcholines were positively related with positive symptoms, and cholic acid was positively associated with negative symptoms. Conclusion Our study provides insights into the metabolite alterations associated with SCZ and potential biomarkers for its diagnosis and symptom severity assessment.

Exogenous Spermidine Promoted Ca2+ Absorption in Lettuce Roots and Reduced the Incidence of Tipburn

Qiao Yang,Na Lu,Lei Wang,Xiao-qing Huang,Da-quan Yang,Jin Sun 한국원예학회 2018 원예과학기술지 Vol.36 No.5

A physiological disorder, called tipburn, commonly occurs in lettuce (Lactuca sativa L.), particularly in greenhouse cultivation and in hot seasons, and reduces its marketability. Calcium (Ca) deficiency is the main cause of tipburn injury. In the present study, we investigated the effect of exogenous spermidine (Spd) on the occurrence of tipburn in hydroponically cultivated head lettuce (var. capitata L.) and elucidated the role of Ca ions (Ca2+) in its prevention with respect to absorption and transport. Ca deficiency in the nutrient solution resulted in reduced uptake of Ca2+ in lettuce roots, thereby resulting in Ca deficiency in leaves, leading to leaf senescence and tipburn. An application of exogenous Spd promoted Ca2+ uptake in lettuce roots under Ca deficiency and promoted Ca2+ transportation to leaves, especially to inner leaves, thus delaying leaf senescence and reducing the incidence of tipburn. The results indicated that application of exogenous Spd is an effective method to decrease tipburn in lettuce caused by Ca deficiency.

Electrostatic contribution to hysteresis loop in piezoresponse force microscopy

Qiao, Huimin,Seol, Daehee,Sun, Changhyo,Kim, Yunseok American Institute of Physics 2019 Applied Physics Letters Vol.114 No.15

Exogenous Spermidine Promoted Ca<SUP>2+</SUP> Absorption in Lettuce Roots and Reduced the Incidence of Tipburn

Qiao Yang,Na Lu,Lei Wang,Xiao-qing Huang,Da-quan Yang,Jin Sun 한국원예학회 2018 원예과학기술지 Vol.36 No.5

A physiological disorder, called tipburn, commonly occurs in lettuce (Lactuca sativa L.), particularly in greenhouse cultivation and in hot seasons, and reduces its marketability. Calcium (Ca) deficiency is the main cause of tipburn injury. In the present study, we investigated the effect of exogenous spermidine (Spd) on the occurrence of tipburn in hydroponically cultivated head lettuce (var. capitata L.) and elucidated the role of Ca ions (Ca<SUP>2+</SUP>) in its prevention with respect to absorption and transport. Ca deficiency in the nutrient solution resulted in reduced uptake of Ca<SUP>2+</SUP> in lettuce roots, thereby resulting in Ca deficiency in leaves, leading to leaf senescence and tipburn. An application of exogenous Spd promoted Ca<SUP>2+</SUP> uptake in lettuce roots under Ca deficiency and promoted Ca<SUP>2+</SUP> transportation to leaves, especially to inner leaves, thus delaying leaf senescence and reducing the incidence of tipburn. The results indicated that application of exogenous Spd is an effective method to decrease tipburn in lettuce caused by Ca deficiency.

Sparse reconstruction of guided wavefield from limited measurements using compressed sensing

Baijie Qiao,Zhu Mao,Hao Sun,Songmao Chen,Xuefeng Chen 국제구조공학회 2020 Smart Structures and Systems, An International Jou Vol.25 No.3

A wavefield sparse reconstruction technique based on compressed sensing is developed in this work to dramatically reduce the number of measurements. Firstly, a severely underdetermined representation of guided wavefield at a snapshot is established in the spatial domain. Secondly, an optimal compressed sensing model of guided wavefield sparse reconstruction is established based on l1-norm penalty, where a suite of discrete cosine functions is selected as the dictionary to promote the sparsity. The regular, random and jittered undersampling schemes are compared and selected as the undersampling matrix of compressed sensing. Thirdly, a gradient projection method is employed to solve the compressed sensing model of wavefield sparse reconstruction from highly incomplete measurements. Finally, experiments with different excitation frequencies are conducted on an aluminum plate to verify the effectiveness of the proposed sparse reconstruction method, where a scanning laser Doppler vibrometer as the true benchmark is used to measure the original wavefield in a given inspection region. Experiments demonstrate that the missing wavefield data can be accurately reconstructed from less than 12% of the original measurements; The reconstruction accuracy of the jittered undersampling scheme is slightly higher than that of the random undersampling scheme in high probability, but the regular undersampling scheme fails to reconstruct the wavefield image; A quantified mapping relationship between the sparsity ratio and the recovery error over a special interval is established with respect to statistical modeling and analysis.

Role of Nucleation-Promoting Factors in Mouse Early Embryo Development

Wang, Qiao-Chu,Liu, Jun,Wang, Fei,Duan, Xing,Dai, Xiao-Xin,Wang, Teng,Liu, Hong-Lin,Cui, Xiang-Shun,Sun, Shao-Chen,Kim, Nam-Hyung Cambridge University Press 2013 Microscopy and microanalysis Vol.19 No.3

<B>Abstract</B><P>During mitosis nucleation-promoting factors (NPFs) bind to the Arp2/3 complex and activate actin assembly. JMY and WAVE2 are two critical members of the NPFs. Previous studies have demonstrated that NPFs promote multiple processes such as cell migration and cytokinesis. However, the role of NPFs in development of mammalian embryos is still unknown. Results of the present study show that the NPFs JMY and WAVE2 are critical for cytokinesis during development of mouse embryos. Both JMY and WAVE2 are expressed in mouse embryos. After injection of JMY or WAVE2 siRNA, all embryos failed to develop to the morula or blastocyst stages. Moreover, using fluorescence intensity analysis, we found that the expression of actin decreased, and multiple nuclei were observed within a single cell indicating that NPFs-induced actin reduction caused the failure of cell division. In addition, injection of JMY and WAVE2 siRNA also caused ARP2 degradation, indicating that involvement of NPFs in development of mouse embryos is mainly through regulation of ARP2/3-induced actin assembly. Taken together, these data suggested that WAVE2 and JMY are involved in development of mouse embryos, and their regulation may be through a NPFs-Arp2/3-actin pathway.</P>