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겔여과크로마토그라피에 의한 질트리코모나스의 항원분획 분석
임미혜,심재찬,민득영,안명희,류재숙,최영길 한양대학교 의과대학 1993 한양의대 학술지 Vol.13 No.1
This study was aimed to analyze the antigenicity of T. vaginalis using a high performance gel filtration chormatography(HPLC), enzyme-linked immunosorbent assay (ELISA) and enzyme-linked immunoelectotransfer blot (EITB). Soluble antigen was prepared using a bead beater. Antiserum was collected from rabbit immunized previously. The results obtained are as follows: the crude antigen of T.vaginalis was separated into 7 peak by HPLC and the fraction 3 revealed the highest reactivity in DLISA. The immunogenic bands of fraction 3 were visualized at 38, 45, 47, 55, 64, 78, 94, 115, 126 and 180 KDa and the bands at 38KDa and 180KDa showed stronger reactivity than that of equivalents of the crude antigen in EITB. In conclusion the fraction 3 of the crude antigen of T. vaginalis obtained by chromatographic separation may have a high antigenicity and proteins ay 38KDa and 180KDa of the fraction 3 may play an important role in antigen-antibody reaction in T. vaginalis infection.
과체중 기혼여성의 비만실태 및 비만에 영항을 미치는 요인
심미자,조명숙,강명심,강연숙 한국미용학회 2001 한국미용학회지 Vol.7 No.2
The main purposes of this study are to measure the obesity level of the married women and examine the factors which have impacts on the obesity of the married women in Taegu area. This study is based on the survey data which were conducted among the married women who regarded them as fat persons from January 1, 200 to February 28, 2000. Cross tabulation analysis, correlation analysis, and regression analysis were introduced for the purposes of this study. In the correlation analysis, it turns out that 12 out of 22 independent variables have statistically significant impacts an the obesity of the married women. However, correlation analysis does not control the influences of the other independent variables on dependent variable. Therefore, we have decided to introduce regression analysis to explore the solid influence of the independent variables on dependent variable. According to the regression analysis, the amount of abdominal fat(β=.8980), the amount of sleeping hours(β=0.1014), regular life style(β=0.1644), and fluid in cell(β =0.1321) have statistically significant positive impacts on the obesity of the married women. On the other hand, age(β=-0.4230) and regular mealtime(β=-0.2310) have statistically significant negative impacts on ,the obesity of the married women. Among the six independent variables, the amount of abdominal fat has the strongest impacts on the obesity of the married women and approximately 80 percent of the variance is explained by the six independent variables in the regression analysis. Finally, the paper is concluded with a discussion of some of the implications to solve the obesity of the married women.
이재명,정춘희,신영구,심명숙,김영욱 대한내분비학회 2001 Endocrinology and metabolism Vol.16 No.1
The 46, XX male syndrome is rare disease that is characterized by a phenotypic male who has a 46, XX female karyotype. Since the first report by de la Chapelle and associates in 1964, several cases have been reportc4 but it is still a rare entity. Recently we examined a 20-year-old XX male who had the symptoms of gynecomastia, an infantile appearance of the external genitalia, scanty pubic hair, no Adams apple, and no axillary hair. We presently describe a patient with the 46, XX male syndrome who showed a 46, XX karyotype on chromosomal study and review the literatures (J Kor Soc Endocrinol 16:148-152, 2001).
박희숙(Park Hee-Sook),이숙자(Lee Sook-Ja),정명자(Jung Myoung-Ja),심정희(Shim Jung-Hee) 한국영유아보육학회 2010 한국영유아보육학 Vol.0 No.62
본 연구는 영유아에게 생태보육활동을 적용하여 영유아의 정서지능에 미치는 효과를 검증하고 그 내용을 알아보는데 목적이 있다. 연구의 대상은 만 2세와 3세의 영유아로 서울의 어린이집 2곳을 선정하여 사전검사를 실시하였다. 3개월에 걸친 생태보육활동 후 영유아의 정서지능을 비교해본 결과, 유의미한 차이가 있는 것으로 나타났다. 즉, 정서지능의 하위 요인인 자기정서의 이용, 타인정서의 인식, 자기정서의 인식, 감정의 조절 및 충동억제, 교사 및 또래와의 관계, 그리고 친사회적 태도에 있어 보육활동의 전과 후 유의미한 차이가 있었다. The purpose of this study was to investigate the effect of the co-centered Early child care program on children's Emotional Intelligence. The subjects of this study were two and three year old children who attend nursery. Pre-examination was applied, and followed by programs in 13 weeks. After program had ended, after-examination was applied. The result of the study is as follows; First, there was a significant difference in children's E.Q. Second, there were significant difference in 6 fields such as Use of Emotions, Emotional Understanding, Emotional Expression, Emotional Management, Interaction with teacher and peers and Social Skills. Third, by a qualitative analysis of observation and descriptive data, we also confirmed the result.
건강검진 수진자 9,137 명에 대한 위내시경적 집단 검진 소견
김상훈,김명숙,박창영,전우규,정을순,손정일,김향,이상종,김병익,최윤상,성기철,심성춘 대한소화기내시경학회 1998 Clinical Endoscopy Vol.18 No.2
Background: A esophagogastroduodenoscopy is now considered to be one of the essential rnethods for diagnosis of upper gastrointestinal disease. Furthermore early detection of stomach cancer by such a mass screening techique is very important to increase the survival rate. Methods: A clinical analysis was conducted on 9,137 healthy subjects who had undergone a gastrofiberscopic examination for the secondary prevention, from January 1995 to December 1996, in Kangbuk Samsung hospital. Results: 1) Number of cases with normal finding was 764 (8.4%). 2) The most prevalent disease found was chronic gastritis (82%). The number of cases with superficial gastritis was 64.7%, erosive gastritis 9.0%, atrophic gastritis 6.4% and metaplastic gastritis 1.6%. Atrophic and metaplastic gastritis were more frequent in the older age group. 3) The prevalence of peptic ulcer was 13.9% and duodenal ulcers (8.1%) was more common than gastric ulcers (5.8%, p$lt;0.05) with a male to female ratio of 3.7: 1, and 2.1: 1 respectively. 4) Of the 10 cases with stomach cancer (0.11%), 5 cases (0.055%) were found to be advanced stomach cancer and the other 5 cases (0.055%) were early gastric cancer. The macroscopic type of early gastric cancer was type IIc in 4 cases and type IIb in another case, and 4 cases were limited in mucosa but another was in submucosa. The size of 4 cases were below 1 cm and there was no lymph node metastasis. Conclusion: Medically screened subjects were found to have many abnormalities, and therefore, it is recommanded that regular check ups using an endoscopy would be needed for early detection of early gastric cancer regardless of clinical symptoms.