서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1997)

김재홍,문득곤,김정수,김용준,임동진,박상훈,김희성,이민수,송기훈,김갑형,김형석,성소영,이인섭,김석우,황지환,조창근,김경문,부태성 대한화학요법학회 2000 대한화학요법학회지 Vol.18 No.3

Background : In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. Objective & Methods : For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results : In 1997. 99 strains of N. gonorrhoeae were isolated, among which 45(45.5%) were PPNG. Conclusion : The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 45.5% in 1997.

Effects of Chungkookjang Extract on Growth Hormone Secretion from GH3 Mouse Pituitary Cell and Growth Hormone Receptor Signaling Pathway

Sun Il Choi(최선일),Ji Eun Kim(김지은),In Sik Hwang(황인식),Hye Ryun Lee(이혜련),Young Ju Lee(이영주),Hong Joo Son(손홍주),Dong Seob Kim(김동섭),Kyu Min Park(박규민),Dae Youn Hwang(황대연) 한국생명과학회 2012 생명과학회지 Vol.22 No.9

뇌하수체 전엽에서 성장호르몬의 생산과 분비는 세포의 분열과 분화 그리고 이동을 조절하는 몇 가지 천연물질에 의해 유도된다. 따라서 발효과정을 통해 제조된 청국장이 성장호르몬의 대사에 미치는 영향을 평가하기 위하여 성장호르몬 분비능과 반응성을 뇌하수체 세포와 성장호르몬 표적세포에서 관찰하였다. 6가지 종류의 콩 품종으로 제조된 청국장 추출물 중에서, 대원, 대풍, 태광의 3종류 청국장 추출물은 5 mg/ml 농도에서 GH3 세포로부터 성장호르몬의 분비를 촉진하였다. 비록 세포 생존능은 이러한 추출물에 의해 유의적인 변화가 유도되지 않았으나, 성장호르몬의 분비량은 청국장 추출물의 농도에 의존적으로 증가하였다. 또한 성장호르몬의 표적 기관으로부터 유래된 MG63과 HepG2 세포는 GH3로부터 수집된 조건적 배양액에 의해 유의적으로 활성화되었다. 또한 이러한 세포에서 STAT5 발현은 대원 청국장 추출물을 처리 후, 15분 혹은 30분부터 세포질에서 유의적으로 증가하였으며, p-STAT5는 핵에서 30분 혹은 60분부터 증가하였다. 따라서 이러한 결과는 3가지 종류의 청국장 추출물은 성장호르몬의 분비를 촉진시키며, 청국장의 조건적 배양액은 성장호르몬 표적세포에서 신호전달을 유도함을 제시하고 있다. The production and secretion of growth hormone (GH) in the anterior pituitary gland can be induced by several natural products to control cell proliferation, differentiation, and migration. To investigate whether Chungkookjang (CKJ) produced by the fermentation process affects GH-related metabolism, the secretion and the response of GH were observed in pituitary cells and GH target cells. Among six CKJs manufactured by different strains of glycine max, only three CKJs, including Daewon (DW), Daepung (DP), and Taegwang (TG), induced GH secretion from GH3 cells at 5.0 mg/ml concentration. There were no significant changes detected in the viability of any of the cells treated with these CKJs. In addition, the increase in GH secretion from the GH3 cells was dependent on the concentration of the three types of CKJs. The proliferation of cell lines, including MG63 and HepG2 cells, that originated from those derived from the GH target organs was significantly activated by treatment with the GH-containing conditional medium (GCM) harvested from the three CKJ-treated GH3 cells, although their induction rate was different from each other. In these cells, p-STAT5 was maximally translocated into the nucleus of MG63 cells 30 min after DW treatment, while it was translocated in HepG2 cells at 60 min. These results suggest that these three types of CKJ could enhance the secretion of GH, as well as the GCM-derived response, in the two target organs.

항정자항체 평가에 있어서 정장액 interleukin-6 측정의 유용성

김민의,박영호,송윤섭 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.2

Breakdown of the blood-testis barrier by infection, trauma, or operation allows sensitization of spermatozoal antigens. Sperm antibodies may be a relative cause of infertility in infertile males. Multiple assay techniques have been developed to detect antisperm antibody. Older tests have been supplanted by the newer immunoglobulin specific assays. Immunobead test was used widely. Interleukin(IL)-6 is necessary for formation of antibody. So, this study was performed to investigate the value of interleukin-6 in the detection of immunologic cause of infertility. The antisperm antibody titers and interleukin-6 was measured in 10 healthy males and 30 patients with chronic prostatitis using immunobead methods and enzyme immunoassay kit. Titers of direct and indirect IgG, IgA and IgM antisperm antibody(% binding) was 0/0.7±0.7, 0/0, 0/0.1±0.1 and 0/1.1±0.5, 0/0.1±0.06, 0/1.1±0.6 in control group and patients with chronic prostatitis. Titers of direct and indirect IgG, IgA and IgM antisperm antibody was 1.8±1.7,0, 0.3±0.3 and 0.3±0.9, 0, 2.8±1.1 in patients with chronic prostatitis whom the antisperm antibody was found in. There was significant difference in indirect IgG and IgM between control group and patients with chronic prostatitis whom the antisperm antibody was found in. The value of seminal IL-6(pg/ml) was 3.2±0.9 and 9.6±2.3 in control group and patients with chronic prostatitis. There was significant difference. The value of seminal IL-6 was 7.0±2.7 and 13.4±5.1 in patients without chronic prostatitis and patients with chronic prostatitis. There was no significant difference. It is suggested that the examination of seminal IL-6 alone can be useful to evaluate the immunologic state of seminal tract but can not take the place of Immunobead test that detect the antisperm antibody formation.

β-mercaptoethanol, hyaluronic acid, EGF가 돼지난포란의 체외수정과 체외발생에 미치는 영향에 관한 연구

김용섭,김민수,김상근 충남대학교 수의과대학 동물의과학연구소 1998 動物醫科學硏究誌 Vol.6 No.-

The study was carried out to evaluate the effects of β-mercaptoethanol(β-ME), hyaluronic acid(HA) and epidemal growth factor(EGF) supplemented to the TCM-199 culture media on in vitro fertilization and in vitro developmental rates of porcine oocytes. The porcine follicular oocytes and embryos were cultured in TCM-199 media containing hormones, β-ME(10, 50, 100 μM), HA (0.5, 1.0, 1.5 ㎎/㎖), EGF(5,8,10%) and PSA(10%) for 40-48 hrs and 8-9 days in a incubator with 5% CO_2 in air at 38.5℃. In vitro developmental rates were defined as development rate on in vitro culture or FDA-test. The results are summarized as follows ; 1. The in vitro feertilization and in vitro developmental rates of porcine oocytes cultured in TCM-199 media supplemented with 10, 50, 100 μM β-ME were 85/113(75.22%), 89/111(80.18%), 92/108(85.19%) and 28/113(24.78%), 36/111(32.43%), 30/108(27.78%), respectively. 2. The in vitro feertilization and in vitro developmental rates of porcine oocytes cultured in TCM-199 media supplemented with 0.5, 1.0, 1.5 ㎎/㎖ HA were 80/103(77.67%), 84/100(84.00%), 82/105(78.09%) and 27/103(26.21%), 35/100(35.00%), 30/105(28.57%), respectively. 3. The in vitro feertilization and in vitro developmental rates of porcine oocytes cultured in TCM-199 media supplemented with 5, 8, 10% EGF were 79/101(78.22%), 86/105(81.90%), 85/103 (82.52%) and 23/101(22.77%), 30/105(28.57%), 32/103(31.07%), respectively. 4. The in vitro feertilization and in vitro developmental rates of porcine oocytes cultured in TCM-199 media supplemented with 10% PSA and 50 u β-ME, 1.0 ㎎/㎖ HA, 8% EGF were 73/85(85.88), 70/80(87.50%), 71/82(86.59%) and 29/85(34.12%), 30/80(37.50%), 25/82(30.49%), respectively. The in vitro fertilization and developmental rates were significantly higher than those of non-supplemented PSA and β-ME, HA, EGF.

Genestone 190 체외충격파쇄석기를 이용한 요로결석 치료의 초기 경험

김민의,송윤섭,박영호,구자현 순천향의학연구소 1998 Journal of Soonchunhyang Medical Science Vol.4 No.2

Shock wave lithotripsy monotherapy was performed in 180 patients with urinary calculi using Genestone 190 lithotriptor between January 1996 and April 1998. Age range of the patients was 17 to 71 years (mean of 44.7 years)and male to female ratio was 2.3:1. There were 54 cases(30%) with renal calculi and 126 cases (70%) with ureteral calculi. NO regional or general anesthesia was required, but parenteral analgesics were required in some patients. The spark voltage was from 16 to 22Kv and average numbers were 2.04 sessions and the mean number of shock per session was 2230±350. The success rates of urinary calculi below 5㎜, 6-10㎜, 11-15㎜, 16-20㎜ and over 21㎜ were 95%, 94%, 91%,75% and 50%, respectively. The success rates of renal, midureter and lower ureter calculi were 85%, 98% and 73%, respectively. There were no significant complications. We think that SWL monotherapy using Genestone 190 lithotriptor is effective and safe procedure for calculi of kidney and upper ureter and calculi below 15㎜.

만성전립선염 환자에 있어서 항정자항체의 존재와 정자의 운동성 감소

송윤섭,김민의,박영호 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.1

Chronic prostatitis may be related to the decrease of semen quality affecting fertility due to the existence of antisperm antibody. So we evaluated the characteristics of antisperm antibody in the control group and patients with chronic prostatitis. Statistical significance was noted in average path velocity(VAP), straight line velocity(VSL), curvilinear velocity(VCL), beat frequency(BCF), elongation of sperm motility parameters between the control group and patients with chronic prostatitis. Detection of antisperm antobody with direct and indirect immunobead test were 0%(0/11), 9.1%(1/11) in the control group and 6.3%(1/16), 37.5%(6/16) in the patients with chronic prostatitis. Titers of antisperm antibodies according to isotype (% binding, control group/ patients with chronic prostatitis) were 0/0.63±2.5(IgG), 0/0(IgA), 0.13±0.5/0(IgM) with direct immunobead test and 0.18±0.60/1.19±1.80(IgG), 0/0.06±0.25(IgG), 0/1.25±2.32(IgM) with indirect immunobead test. Statistical significance was noted in VAP, VCL, BCF between chronic prostatitis patients with and without antisperm antibody. The correlation between IgG and VAP, IgG and VCL existed. In conclusion, decreased sperm motility and the antispermantibody were found in chronic prostatitis. The antisperm antibody showed sharp contrast between the control group and the patients by indirect immunobead test, rather than by direct immunobead test. The existence of antisperm antibody correlated with sperm motility in chronic prostatitis.

국내 발전소별 바텀애시를 잔골재 대체재로 사용한 모르터의 특성에 대한 실험적 연구

송민섭,장재봉,조봉석,김재환,김용로,김무한 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1(구조계)

With the advance in industrial development and modern living comfort, the demand for electricity throughout the country is ever increasing. Also the annual consumption of coal by coal-fired power plants is increasing. The amount of ash produced in 2000 is estimated to be about 4.5 million tons. The disposal of this huge amount of ash introduces serious environmental problems. Fly ash among the ash produced is used in building industries as a substitute to cement in concrete and the concrete thus produced is said to be lighter and stronger. But bottom ash among the ash produced is not used because of its poor properties. But encouraging the use of bottom ash as a construction material is a sensible method of utilization as it avoids the problems and costs associated with disposal and provides an alternative aggregate source. This study is aimed at using bottom ash as an alternative fine aggregate source to provide a solution to disposal and insufficient fine aggregate for the production of concrete. So properties of bottom ash produced in power plants were estimated due to the difference of bottom ash produced in each plant. And mortar strength was estimated as basic data to use bottom ash in building industries.

왜소음경증과 남성 성기능에 관한 연구

송윤섭,김민의,이남규,박영호,전윤수,김응구 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.2

Micropenis is defined as a normally formed penis with a size greater than 2 standard deviations below the mean. Twenty three patients with micropenis were managed at Soonchunhyang University Hospital from January 1988 to December 1995 We interviewed and examined them with micropenis to investigate the cause of micropenis, concomitant diseases, the efficacy of treatment and their sexual activity in postpubertal men. The patients were divided into 2 groups: 16 prepubertal and 7 postpubertal men. 9 patients had cryptorchidism, 3 of them were bilateral and 5 patients had hypospadias. Etiology was determined by hormonal assay including HCG stimulation test ; 8 as hypogonadotrophic hypogonadism, 4 as primary testicular dysfunction and 5 as idiopathic. However etiology was undetermined in 6 patients. All patients had endocrine therapy using HCG or depot testosterone. All except 4 cases responded satisfactorily. All postpubertal patients(group 2) were below the 10th percentile. Puberty was delayed in 3 patients in the older group. All patients were heterosexual and they have had erections and orgasms. Five patients have had ejaculations, 3 of them were sexually active and they have experienced vaginal penetration, Nevertheless, their sexual partners have had complaints and the patients themselves have felt ashamed and dissatisfied. Fortunately, they were mentally healthy. None of these patients were married or had a child.

발기부전 검사에서 천연색 복합초음파촬영술과 야간음경발기검사의 일치도

송윤섭,구자현,김민의,이혜경,김두상,박영호,이남규 순천향의학연구소 1999 Journal of Soonchunhyang Medical Science Vol.5 No.1

We intended to know that the result of color duplex ultrasonography corresponded to that nocturnal penile tumescence rigidity in the diagnosis of erctile dysfunction. We performed both color duplex ultrasonography and nocturnal penile tumescence rigidity in 15 erectile dysfunction men. Colr duplex ultrasonography was done with a 7 MHz. color Doppler unit after intracorporeal pharmacological injection and measured maximal arterail diameter, peak systolic velocity, end-diastolic velocity and the results were compared with rigidity and tumescence in nocturnal penile tumescence rigidity using RigiScan. The positive predictive value, negative predictive value, sensitivity, specificity, overall accuracy of color duplex ultrasonography was reasonable when the result of color duplex ultrasonography was compared with the that of nocturnal penile tumescence rigidity. According to this results, color duplex ultrasonography could not only predict but also correspond to nocturnal penile tumescence. Therefore, we suggest that color duplex ultrasonography can relpace nocturnal penile tumescence rigidity if psychogenic and neurogenic erectile dysfunction are distinguished by history and neurologic examination.