심근경색의 위험인자로서 Thrombomodulin유전자변이의 의의

박현영,김영미,권혁문,지선하,조승연,정남식,심원흠,장양수 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.6

노인의 영적 간호요구

최미혜,김경희,김귀옥,김기숙,김수강,김정신,김춘숙,노흥진,박지연,성혜연,오명선,이선희,이원옥,이윤영,이현수,장명재,차혜경,채정선,홍상희 중앙대학교 의과대학 간호학과 간호과학연구소 2001 중앙간호논문집 Vol.5 No.1

This study was designed to exam the aged's needs for spiritual nursing care. The purpose was to serve as a basis for the development of spiritual nursing practice. The major findings are as follows : 1. The degree of needs for spiritual nursing care as area was that needs of love and relationship mean 22.0, needs of meaning and object mean 28.2, needs of forgiving mean 13.5. Total needs for spiritual nursing care mean 63.7, which was on the upper middle level. The needs of meaning and object was rated highest. 2. Among the general characteristics of the subjects, needs of love and relatiohship wasn't significanlty different. 3. Among the general characteristics of the subjects, needs of meaning and object was significantly different according to two factors : age(F=7.260, p=0.001), religion(F=5.275, p=0.001). Higher needs of meaning and object was possessed by the older than the younger, by the one who have religion than the other. 4. Among the general characteristics of the subjects, four factors made a significantly difference to needs of forgiving : sex(t=-2.851, p=0.006), age(F=8.201, p=0.001), religion(F=6.928, p=0.000), disease(t=2.327, p=0.024). Higher needs of forgiving was possessed by man than woman, by the older than the younger, by the one who have religion than the other, by the one who have disease than the other.

마황윤폐탕의 병용이 Quinolone계 항생제 중 Rufloxacin의 호기성 Gram(+) 세균주에 대한 시험관 내 항균력에 미치는 영향

박미연 ( Park Mi Yeon ),김대준 ( Kim Dae Jun ),김종대 ( Kim Jong Dae ) 대한본초학회 2003 大韓本草學會誌 Vol.18 No.4

N/A Objectives : In order to evaluate the in uitro synergic effect of Mawhngympye-tang which was a traditional poly-herbal formula has been used in the treatment of respiratmy diseases in Korea, with quinolone antibiotics, rufloxacin the minimal inhibitory concentration (MIC), MI& and MIGO of single use of quinolones and concomitant treatment with Mawhangyoonpye-fnng against 9 strains of gram positive bacteria. Methods : Agal plate dilution methods. Results : 1. In the case of aerobic gram positive bacteria, the MIC, MI& and MI& against S@&loconus aweus, Stqphyl- aweus smith, Staphyl- epidermidis, SWwlocoaxrs pyogens, Streptoamus pneumoniue Type I, Type II and Type III was significantly decreased in concomitant treated groups with Mawhrutgyoonpye-tang compared to those of single treated groups of RUFX. Conclusions : It is considered that the in uitro antibacterial activity of RUFX were increased against some strains of gram positive strains, especially, pneumococcus such as Stc&~loaxxus, Streptococcus by concomitant use of Mawwoorapye- tang.

Distribution of Aquaporins (Water Channels) in the Rat Salivary Glands

정지연(Ji-Yeon Jung),한창룡(Chang-Ryoung Han),정연진(Yeon-Jin Jeong),오원만(Won-Mann Oh),김미원(Mi-Won Kim1),김선헌(Sun-Hun Kim),김옥준(Ok-Joon Kim),김현진(Huyn-Jin Kim),고정태(Jeong-Tae Koh),최홍란(Hong-Ran Choi),김원재(Won-Jae Kim) 대한해부학회 2002 Anatomy & Cell Biology Vol.35 No.3

침샘은 하루 1.5 l의 침을 분비하며, 일반적으로 침샘에서 수분이동은 능동적 염분 이동에 따른 삼투현상에 의해 일어난다. 따라서 침샘에서 높은 수분투과성으로 인해 수분통로 단백인 aquaporin (AQP)들이 풍부하게 존재할 수 있다. 지금까지침샘에 4가지 형태의 AQP이 존재한다고 알려져 있지만 침샘에서 정확한 위치와 수분 이동에 대한 각 AQP들의 역할은 아직 확실치 않다. 본 연구는 300 g 정도의 흰쥐를 pentobarbital sodium (50 mg/kg, IP) 마취하에서 carbarchol (10 μg/kg)을 복강 내 투여한 후 AQP들의 역할과 분포를 면역조직화학방법으로 조사하였다. AQP1은 침샘의 미세혈관의 내피세포와 샘세포와 샘관 주위에 존재하는 근육상피세포에 존재하였다. AQP4는 침샘관의 마지막 부위인 배출관에 존재하였다. AQP5는 주로 장액세포의 바닥가쪽과 세포사이 분비소관을 포함한 세포꼭대기쪽막 에 존재하였으며 콜린성 분비 자극으로 세포꼭대기쪽막으로 이동되어 밀집되었다. AQP5는 모든 침샘의 사이관과 줄무늬 관에도 존재하였으며 점액세포는 약하게 존재하였다. AQP8은 AQP5처럼 장액세포의 바닥쪽막과 세포사이 분비소관을 포함한 세포꼭대기쪽막에 존재하였으며 콜린성 침분비 자극으로 세포꼭대기쪽막쪽으로 이동되어 밀집되었다. 이상의 실험결과는 AQP5와 AQP8이 장액세포에서 일차 침 형성시 일어나는 수분이동의 주된 통로임을 시사하였다. The salivary glands produce 1.5 l of fluid per day. As in other organs, the general paradigm in the salivary glands is that water movement occurs secondary to osmotic driving forces created by active salt transport. Therefore, high water permeability in salivary glands is expected to need a variety of aquaporin (AQP), a water channel. Although four AQPs have been known to reside in salivary glands, the precise location and roles of AQPs have been not well examined. This study is aimed to investigate the distribution of AQPs in 3 major salivary glands and their changes after cholinergic stimulation using immunohistochemical study in Sprague Dawley rats weighing 300 g under pentobarbital sodium anesthesia. AQP1 was localized in the endothelial cells of all salivary capillary vessels and the myoepithelial cells. AQP4 was demonstrated in the epithelium of the excretory ductal cells of all salivary glands. AQP5 and 8 were abundantly present in the basolateral membrane and apical membranes of the serous acini including intercellular secretory canaliculi, whereas AQP5 was weakly present in mucous acini. In addition, AQP5 was found in the epithelium of the intercalated and striated ducts. Upon stimulation of carbachol (10 μg/kg, I.P). AQP5 and 8 tended to translocate from basolateral membrane to the apical membrane, appearing as clusters of dots. These results suggest that AQP5 and 8 are the candidate molecules responsible for the water movement in salivary acinar cells.

남성 근로자의 고혈압 유무에 따른 열량 영양소 및 지질 섭취 상태 비교

김연정,김수근,백윤미,김미경 대한산업의학회 2006 대한직업환경의학회지 Vol.18 No.4

목적: 본 연구에서는 정상 근로자와 고혈압인 근로자간의 식이섭취 실태의 차이를 파악하여 효과적인 사업장 고혈압 관리 방안에 대한 기초 자료를 얻고자 하였다. 방법: 연구 대상은 임의 추출한 발전소 근무자 427명을 대상으로 하였으며, 신장, 체중과 혈압을 측정하고, 생활습관 및 식품섭취빈도조사를 실시하여 자료가 완전한 338명을 분석하였다. 대상자를 정상, 경계 고혈압과 고혈압군으로 분류하여 각 군 간 연령, 신장, 체중, 체질량지수, 혈압 등 인체계측 결과와 흡연, 음주, 운동 등 생활습관 및 열량 영양소 섭취 상태, 지질 성취상태의 결과를 비교하였다. 결과: 연령과 신장은 세 군 간 차이가 없었으나 체중과 체질량지수는 고혈압 군과 다른 두 군 간에 유의한 차이를 보였다(p<0.05). 세 군 모두 흡연율은 40.0% 미만으로 차이가 없었으나 흡연량은 경계 고혈압과 고혈압군이 정상군보다 유의하게 더 많았다(p<0.05). 음주 습관에서 음주 빈도와 음주량은 경계 고혈압군이 다른 두 군보다 더 높았으며, 음주량만 통계적으로 유의한 차이가 있었다(p<0.05). 운동 빈도는 주3회 이상 하는 경우가 정상군에서 높았으나 세 군 간에 차이가 없었다. 총 열량 섭취량은 세 군 간 차이가 없었으나, 탄수화물, 단백질, 지방 섭취량과 3대 영양소의 에너지 섭취 비율은 세 군 간에 유의한 차이가 있었으며, 고혈압 군이 다른 군 보다 탄수화물의 섭취는 많고, 지방 섭취는 적었다(p<0.05). 정상군에 비해 경계 고혈압과 고혈압군이 콜레스테롤 섭취량이 상대적으로 적었으나, 유의한 차이가 없었다. P / M /S비도 세 군 간에 유의한 차이가 없었다. 경계 고혈압군은 총 열량, 단백질, 지방 과잉 섭취자의 비율이 높았고, 고혈압군은 탄수화물, 지방 과잉섭취자의 비율이 높았으나 통계적으로 유의하지는 않았다. 결론: 본 연구에서 정상 근로자와 고혈압 환자 간에 탄수화물 섭취에 유의한 차이가 있었으며, 경계 고혈압과 고혈압군에서 열량 영양소 섭취와 지방산 섭취가 상대적으로 불균형하였다. 이러한 연구결과에 따라 고혈압뿐만 아니라 경계 고혈압단계에 있는 근로자를 조기에 발견하여 식습관을 포함한 생활습관을 개선하도록 관리할 필요가 있다. Objectives: The purpose of this study was to investigate the difference in nutrient intake between normotensive workers and hypertensive workers to obtain basic data for developing a program of effective hypertension care at work. Methods: The subjects were 338 male workers. We measured physical data by medical examination and collected lifestyle data by questionnaire survey. Dietary intakes were collected using a food frequency questionnaire. We classified the subjects into normal, prehypertension, and hypertension groups, and compared the results of age, body mass index (BMI), blood pressure (BP), lifestyles and energy nutrients and lipids intake. Results: There were significant differences in both weight and BMI between the hypertension group and the other two groups (p<0.05). The smoking levels of the prehypertension group and hypertension group were significantly higher than that of the normal group (p<0.05). The three groups also showed significant differences in drinking amount (p<0.05). There were significant differences in carbohydrate and protein intakes and the ratio of carbohydrate, protein, and fat intake (p<0.05). Conclusion: In this study, there was a significant difference in carbohydrate intake between normotensive and hypertensive workers, and the intake of energy nutrients and fatty acids in the prehypertension group and hypertension groups was unbalanced. Therefore, early diagnosis of workers with hypertension and prehypertension is necessary to improve lifestyles particularly food habit.

Identification of a sensitive urinary biomarker, selenium-binding protein 1, for early detection of acute kidney injury

Kim, Kyeong Seok,Yang, Hun Yong,Song, Hosup,Kang, Ye Rim,Kwon, JiHoon,An, JiHye,Son, Ji Yeon,Kwack, Seung Jun,Kim, Young-Mi,Bae, Ok-Nam,Ahn, Mee-Young,Lee, Jaewon,Yoon, Sungpil,Lee, Byung μ,Kim, Hyung TAYLOR & FRANCIS 2017 Journal of Toxicology and Environmental Health Vol.80 No.9

<P>Acute kidney injury (AKI) is associated with increased mortality rate in patients but clinically available biomarkers for disease detection are currently not available. Recently, a new biomarker, selenium-binding protein 1 (SBP1), was identified for detection of nephrotoxicity using proteomic analysis. The aim of this study was to assess the sensitivity of urinary SBP1 levels as an early detection of AKI using animal models such as cisplatin or ischemia/reperfusion (I/R). Sprague-Dawley rats were injected with cisplatin (6 mg/kg, once i.p.) and sacrificed at 1, 3, or 5 days after treatment. Ischemia was achieved by bilaterally occluding both kidneys with a microvascular clamp for 45 min and verified visually by a change in tissue color. After post-reperfusion, urine samples were collected at 9, 24, and 48 hr intervals. Urinary excretion of protein-based biomarkers was measured by Western blot analysis. In cisplatin-treated rats, mild histopathologic alterations were noted at day 1 which became severe at day 3. Blood urea nitrogen (BUN) and serum creatinine (SCr) levels were significantly increased at day 3. Levels of urinary excretion of SBP1, neutrophil gelatinase-associated lipocalin (NGAL), and a tissue inhibitor of metalloproteinase-1 (TIMP-1) were markedly elevated at day 3 and 5 following drug treatment. In the vehicle-treated I/R group, serum levels of BUN and SCr and AST activity were significantly increased compared to sham. Urinary excretion of SBP1 and NGAL rose markedly following I/R. The urinary levels of SBP1, NGAL, TIMP-1, and KIM-1 proteins excreted by AKI patients and normal subjects were compared. Among these proteins, a marked rise in SBP1 was observed in urine of patients with AKI compared to normal subjects. Based upon receiver-operator curves (ROC), SBP1 displayed a higher area under the curve (AUC) scores than levels of SCr, BUN, total protein, and glucose. In particular, SBP1 protein was readily detected in small amounts of urine without purification. Data thus indicate that urinary excretion of SBP1 may be useful as a reliable biomarker for early diagnosis of AKI in patients.</P>

신규간호사와 프리셉터가 인지하는 프리셉터의 교수효율성 비교

김지양,김영선,김춘실,박현숙,신미영,윤연숙,조유숙,박미미,유문숙 한국간호과학회 간호행정학회 2009 간호행정학회지 Vol.15 No.2

Objective: The purpose of this study was to compare preceptors and new graduate nurses on their perception of preceptor teaching effectiveness. Methods: The participants were 90 new nurses and 90 preceptors who worked in A medical center. The data were collected from July 1 to September 30, 2008. A cross-sectional descriptive survey was done using a structured questionnaire. Results: New nurses' perception (4.07±.44) of the preceptor teaching effectiveness was significantly higher than perception of the preceptors (3.57±.37). Fifty five percent of new graduate nurses reported a lack of coherence in the preceptor's practice guidelines. For 5 causal factors classified on the teaching effectiveness, the factor of 'Professional knowledge and ability' showed the highest score, but 'Interpersonal and communication skill' got lowest score for both group. Preceptors responded that they did not have enough time to teach well because of their heavy workloads. Conclusions: These results suggest that the preceptors need appropriate compensation and education opportunities, and new graduate nurses need consistent education by the teaching professionals. Therefore, it is important to give preceptors full charge of the preceptorship. Also, it will be necessary to develop education programs to enhance interpersonal and communication skill for preceptors and new nurses.

단클론항체를 이용한 타액 내 Streptococcus mutans 수준의 측정

김추성,김재곤,양연미,백병주,이경열,김미아,임수민 大韓小兒齒科學會 2010 大韓小兒齒科學會誌 Vol.37 No.2

Streptococcus mutans는 구강 내에 상존하는 치아우식증의 주요 원인균으로서 치면의 피막에 부착 후 glucan을 형성하여 세균의 군락을 이루며, 외부로부터 공급된 자당대사를 통하여 유기산을 생성함으로써 법랑질을 탈회시킨다. 치아우식 활성도의 평가를 위한 단클론항체를 이용한 방법은 진료실에서 빠른 시간 내에 간편하게 타액에 존재하는 Streptococcus mutans의 정량분석이 가능한 방법이다. 이 연구는 3세에서 6세 사이의 어린이 15명을 대상으로 자극성 타액을 채취하여 시판 중인 단클론항체를 이용한 Salivacheck Mutans, strip을 이용한 Dentocult-SM 그리고 MSB배지 배양법으로서 타액 내 Streptococcus mutans를 측정한 후 그 값을 우식경험치아수와 비교하여 상관관계를 알아보았다. Saliva-check Mutans를 이용한 방법은 Dentocult-SM과 MSB배지법과 통계학적으로 유의한 상관관계를 보였으나 (p<0.05), MSB배지법은 어린이의 우식경험치아수와 통계학적으로 유의한 결과를 나타내지 않았다 (p=0.34). Streptococcus mutans, one of the major causal agents of dental caries, is component of the dental plaque. It produces various organic acids such as lactic acid which is the end-product of glycolysis, and this leads to dental caries. A new system using species-specific monoclonal antibodies was developed to detect Streptococcus mutans in saliva. The system quickly detects salivary Streptococcus mutans in 30min and classifies the result into two levels. The purpose of this study was to investigate correlation between monoclonal antibody-based detecting system and selective medium-based detecting methods. Children's deft indices were also compared with Streptococcus mutans counts in MSB agar plate. Subjects consisted of 15 children in the age of 3 to 6 years. They were assigned to three groups: Group Ⅰ (deft index = 3), Group Ⅱ (deft index ≤ 3), Group Ⅲ (deft index ≥ 4). The results are as follows : 1. The rate of children with positive response was 13.3% and with negative response was 86.7% in the result of Saliva-check Mutans test kit. 2. There was a positive correlation between monoclonal antibody-based detecting system and selective medium-based detecting methods (p<0.05). 3. Streptococcus mutans counts in MSB agar plate were irrelevant to deft of children(p=0.34).

역행성 심정지 도관 삽입을 위한 술 중 경식도심초음파의 유용성

유미숙;최원주;김정원;박장수;김경태;김지연;어상일 인제대학교 2011 仁濟醫學 Vol.32 No.-

Background: Retrograde cardioplegia catheter (RCC) insertion is one of methods for administration of cardioplegics during cardiopulmonary bypass (CPB). However its success rate depends on only surgeon's experience. We thought the transesophageal echocardiography (TEE) can guide the R CC insertion, because TEE is commonly used for monitoring status of heart during cardiac surgery. This study was designed to evaluate the RC C insertion time by TEE guidance versus conventional method without T EE guidance. Methods: Sixty patients who were scheduled to undergo elective coronary artery bypass graft (CABG) surgery under general anesthesia were divided into groups as follows: group T [RCC insertion into coronary sinus (CS) by TEE guidance, n=30] and group C [RCC insertion into CS by conventional method, n=30]. We recorded the time of insertion of RCC in to CS in two groups. RCC insertion was done by TEE guidance in case that time was over two minutes by conventional methods in group C. Results: The overall time of RCC insertion into CS was 63.8 ± 23.6 seconds in the Group T and 102.3 ±55.7 seconds in the Group C, respectively (p=0.001). There were no complications during RCC into CS by T EE guidance. Conclusions: TEE guidance for RCC insertion into CS is shorten the time of insertion.

비전문가를 위한 실내 공간 음향설계 도구 개발에 관한 연구

김미연(Mi-Yeon Kim1),이윤길(Yun-Gil Lee) 호서대학교 공업기술연구소 2011 공업기술연구 논문집 Vol.30 No.1

본 연구는 건축 공간의 실내음향 설계에 있어 보다 효율적이고 비전문가도 쉽게 활용할 수 있는 실내 음향 공간 모델링 및 음향분석 시뮬레이션 시스템을 개발하고 이를 활용한 새로운 음향 분석 절차의 제안 하는 것을 목표로 하고 있다. 이러한 시스템의 개발은 기존의 선형적인 실내 음향 공간 디자인의 문제점을 극복하고 건축음향 공간의 최적화를 위한 나선형 건축음향 모델링 및 분석 체계를 이루는 기반이 된다. Recently, the social desire in Cultural liiew is increased according to the wide-spread or Well-being Culture . In this manner, the necessity of good acoustic design of enclosed spaces is raised. In order to satisfy the citizen with cultural desire, the government has planed to create the recreation spaces such as auditoriums and theatres. Those spaces where sound quality is usually low are reproduced because of an inadequate acoustic design. Therefore, the development of a powerful acoustic design tool for public spaces is needed Nowadays, most of the existing acoustic simulation tools are always expensive and hard to use. As the result, our development in acoustic simulation tools aims on creating a local and user-friendly tool ^lich everyone can use. In tins manner, we suggest new acoustic design process using a expert design system using material styles and the space model library fra* the nonprofessional designer.