심근경색 모델 흰쥐에서 스트레스가 B-type Natriuretic Peptide 발현 및 심박변이율 변화에 미치는 영향

이삼윤(Sam Youn Lee),이미경(Mi Kyoung Lee),김남호(Nam-ho Kim),주민철(Min Cheol Joo),조항정(Hyang Jeong Jo),강지숙(Ji Sook Kang),김병숙(Byung Sook Kim),윤선식(Sun Sik Yoon),최을식(Eul Sig Choi),이문영(Moon Young Lee) 대한스트레스학회 2010 스트레스硏究 Vol.18 No.3

심박변이율의 감소는 심근 경색 환자에서 예후의 악화와 관련되어 있다. 심박변이율 중 very low frequency가 심부전환자의 예후를 판단할 수 있는 독립적 인자로서 사용이 가능하다고 보고된 바 있으며, 심실의 압력 증가에 반응해서 생성되는 B-type natriuretic peptide가 심근 경색의 예후 인자로서 사용할 수 있음 역시 보고된 바 있다. 본 연구에서는 심근경색 모델을 제작하여 일정의 회복 기간을 거친 다음 다시 일정 기간의 스트레스를 겪게 한 후 심박변이율의 변화 및 심실 조직에서의 BNP 발현 정도를 비교하여 스트레스에 의한 영향을 관찰하고자 하였다. Sprague-Dawley계 수컷 흰쥐 15마리를 대상으로 하였다. 실험동물은 인위적 스트레스 및 수술을 받지 않은 대조군(CON, n=5), 심근경색 수술을 받은 후 restraint stress를 받지 않은 군(MI+No Stress, n=5), 심근경색 수술을 받은 후 1주일 동안 restraint stress를 받은 군(MI+Stress, n=5)으로 분류하였다. 심근경색 모델 제작 직후 15분 이상 심전도의 변화를 기록하였으며, 2개월 정도 후 심근경색 수술을 받은 동물을 두 군으로 나누어 그 중 한 군에는 1주일 동안 restraint stress를 가한 후 restraint stress를 가하지 않은 동물과 심박변이율을 비교 분석하였다. 심실 조직은 좌심실 전벽 부위의 위축을 관찰할 수 있었고, 대식세포에 의하여 응고, 괴사된 심근세포의 탐식과 혈관이 풍부한 육아조직 및 섬유 변화를 볼 수 있었다. 심박변이율은 심근경색 수술을 받은 직후 심박수는 유의한 증가를 보였고, standard deviation of the normal to normal intervals (SDNN), very low frequency (VLF) 및 low frequency (LF)의 유의한 감소를 보였다. 심근경색 수술을 받았던 동물에서 두 달 정도의 회복 기간을 거친 후 스트레스를 받지 않은 군은 심박수 및 기타 심박변이율 분석에서 SDNN 값을 제외하고는 정상군과 유의한 차이를 관찰할 수 없었던 반면 1주일 동안 스트레스를 받은 군에서는 심박수가 다시 유의하게 증가했을 뿐만 아니라 SDNN, VLF 및 LF 역시 정상군과 유의한 차이를 보였다. 이상의 결과를 종합하면 심근경색 동물에 대해 1주일 동안 스트레스를 가한 결과 심박변이율이 감소하고 심실에서의 BNP 발현은 더욱 증가하여 악화함을 보여주고 있다. Diminished heart rate variability (HRV) is associated with less favorable prognosis after myocardial infarction (MI). It has been reported that very low frequency (VLF) power in HRV analysis is an independent risk predictor in patients with congestive heart failure and B-type natriuretic peptide (BNP) can be used as a prognostic factor of MI. The purpose of this study was to investigate whether stress has an effect to the changes of BNP expression and/or heart rate variability in MI model in rats. Male Sprague-Dawley rats were divided into 3 groups: control group (CON), MI group (MI+No Stress), and MI followed by stress group (MI+Stress). MI+Stress group rats were raised for a two month recovery period after the operation, followed by being exposed to restraint stress for 2 hours per day for 1 week. Electrocardiogram was recorded after the operation and the last day after 1 week of stress. The frequency components of HRV were calculated in the frequency domain such as VLF, low frequency (LF), high frequency (HF) and so on. In HRV analysis, standard deviation of the normal to normal intervals (SDNN) was significantly reduced in both groups compared to the control group. VLF and LF also were significantly reduced in MI+Stress group compared to the control groups. In addition, BNP expression in western blotting was shown the strongest bands in MI+ Stress group among experimental groups. These results suggest that BNP and HRV were aggravated by stress in MI rat model. (Korean J Str Res 2010;18:275∼285)

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

임상간호사의 사회심리적 건강과 건강지각, 건강관련 행위

김정희,현미열,김영순,김정실,남상옥,송만숙,이라주,이정화 한국간호과학회 간호행정학회 2010 간호행정학회지 Vol.16 No.1

Purpose: The purpose of this study was to investigate the relationships among psycho-social well-being, and health perception and behavior in clinical nurses. Methods: The data were collected from 362 nurses employed at five general hospitals. A self-reported questionnaire was used to assess demographics and job characteristics, health perception and behavior, depression, and psycho-social stress. The data were analysed using Cronbach's alpha, descriptive statistics, t-test, and ANOVA. Results: While nurses perceived themselves to be in good health, represented by a high mean for health perceptions, actual health behavior was poor as the mean for health behavior was low. Forty two percents of the subjects were categorized as having major depression and high-stress levels. There were statistically significant differences in psycho-social well-being according to age, marital status, years of employment, and monthly salary. Also, there were significant differences in psycho-social well-being according to health perception, regular meals and exercise, sufficient sleep, job related injury and accident, and absences for ill. Conclusion: In order to prevent depression and improve psycho-social well-being among clinical nurses, organizational support and health management needs to be focused on high-risk nurses. Furthermore, it is essential to consider the health perception and behavior of clinical nurses.

거제지역 당뇨병 및 고혈압 환자의 복합질병 위험요인

박필숙,정기만,김종현,백지현,박미연 한국식생활문화학회 2003 韓國食生活文化學會誌 Vol.18 No.2

The purpose of this study is to compare the correlation of clinical characteristics and patterns of disease. Subjects of the study were the adults(207) living in Geoje City, the diabetes mellitus and the hypertension patients(166) and the normal people(41). In the diabetes mellitus group and the hypertension group, blood pressure, blood glucose, total cholestero LDL-cholesterol and atherogenic index(AI) were significantly high. As the obesity index was getting higher, the blood pressure of the diabetes mellitus group was high, and the HDL-cholesterol of the hypertension group was low, but AI of it was significantly high. The AI was significantly high as serum lipid index were getting higher in both groups. The rate of the prevalence was very high in the diabetes mellitus group(74.3%) and the hypertension group(73.7%). The pattern in the diabetes mellitus group was in order of the hypertension, the hyperlipidemia, and the obesity but, in the hypertension group was the hyperlipidemia, and the obesity. The obesity index and serum lipid index of complex patient group were higher than single patient group.

성별에 따른 대학생의 내면화된 수치심과 대인관계 문제가 우울증상에 미치는 영향

최재정,김미연,박민지,유미숙 숙명여자대학교 아동연구소 2015 兒童硏究 Vol.27 No.1

본 연구는 대학생의 내면화된 수치심과 대인관계가 우울증상에 미치는 영향력을 규명하고자 한 연구이다. 연구 대상은 서울, 경기 지역의 대학생 404명을 대상으로 자기보고식 설문조사를 실시하였다. 본 연구에 사용된 측정도구는 대학생의 내면화된 수치심, 대인관계, 우울증상의 척도였다. 분석방법은 SPSS 19.0 통계프로그램을 이용하여 빈도와 백분율을 산출하였고, 내적합치도 계수(Cronbach a)를 구하였다. 본 연구문제를 여자 검증하기 위해 중다회귀분석과 단계적 회귀분석을 실시하였고, 밝혀진 결과를 요약하면 다음과 같다. 첫째, 여자 대학생은 사회적 회피와 헌신성이 우울증상과 관계가 있는 것으로 나타났다. 둘째, 남자 대학생은 실수 불안과 지배성, 비주장성이 우울증상에 영향을 미치는 것으로 나타났다. 셋째, 여학생과 남학생의 결과 차이에서 모두 우울 증상에 유의미한 영향을 미치는 것으로 나타났고, 수치심의 하위요인보다 대인관계문제의 하위요인들이 우울 증상에 보다 많은 영향을 미친다. 수치심보다 대인관계 문제가 우울 증상에 보다 큰 영향을 미친다는 결과는 남녀 대학생 모두에게 공통적으로 나타났다.

크릴분말이 콜레스테롤 및 카드뮴 식이 흰쥐의 간 및 심장 지질에 미치는 영향

조영숙,박석규,박정로,손미예,문주석,곽수동 순천대학교 기초과학연구소 1992 基礎科學硏究誌 Vol.3 No.-

In order to investigate effects of krill on lipid components of liver and heart in cholesterol and cadmium-fed rats, male Sprague-Dawley rats were raised for 4 weeks with 8 experimental diets. Krill used in this experiment contained 65.4%(w/w) of protein and 16.7% of lipid. Krill contained 35% of polyunsaturated fatty acids and more unsaturated fatty acids than saturated fatty acids by 2 times. Amino acid content of krill was high in order of Glu, Asp, Tyr and Lys. Rats fed krill diet showed more food intake and weight gain compared with control group. However, food efficiency ratio and weight of liver and heart were not significantly different. The ingestion of cadmium resulted in a severe restriction in growth rate with normal or mild reduction of liver and heart weight. Cadmium also caused a significant decrease in food efficiency ratio. The contents of total and free cholesterol of liver and heart in rats fed krill diets were similar or slightly higher than those fed control diet. Dietary krill also showed no significant difference in liver and heart cholesterol levels in rats with cadmium ingestion. The contents of phospholipid and triglyceride in liver and heart of krill group were slightly higher than those of control group. Supplement of krill reduced triglyceride content of liver in rats ingested with cadmium without any effect on the level of phospholipid and heart lipids. The concentration of cadmium in plasma significantly increased with dietary krill. However, the krill supplement did not influence the concentration of cadmium in liver or heart. A significant accumulation of lipid in liver tissue was observed in all dietary groups but standard group. However, no difference in degree of lipid accumulation was found among the dietary groups. Necrosis and hemolysis of liver in all dietary groups were not shown.

노인복지정책의 문제점과 개선방안에 관한 연구

손경숙,정미녀 三陟大學校 2004 論文集 Vol.37 No.-

In the population structure of our society due to the development of medical technology: which liberated many from various diseases, and diversified modem society, the average life span has extended greatly. and this has drawn much attention to social welfare for the elders which became an important matter. Many geriatric problems have floated due to change of family structures and values and changes of life style which is resulted from industrialization and urbanization in the modem days. This has created the lowering of social status for older generation which has resulted as a serious social problem. However: countermeasures taken from the government is very mere and still in the beginning level. In this sort of situation, taking a good look at all sorts of older generation isn\'t a somebody else\ story. This would have a direct effect on you, and would like to present an alternative plan for this problem. The effort of trying to make a better solution to this problem! would result in much more effective and prosperous perspective on the area of geriatric social welfare policies.

전분 종류를 달리한 무화과편의 품질 특성

김병숙,정미란,이영은 한국조리과학회 2003 한국식품조리과학회지 Vol.19 No.6

The effects of various kinds of starch (mungbean, potato, corn, and a blend of potato and corn starch) on the quality characteristics of muhwakwa-pyun were studied according to the storage duration. The sweetness, pH and total acidity of muhwakwa extract were analyzed, as were the Visco/Amylograph viscosity profile and DSC thermodynamic characteristics of starches. For quality characteristics, color difference, instrumental texture characteristics and sensory characteristics were compared. The sweetness of muhwakwa extract was satisfactory but the total acidity was low for Kwa-pyun manufacture. Therefore, its pH was adjusted to 3.4 by adding 10% citric acid with an amount of 1% of total liquid. From the Visco/Amylograph viscosity profile and DSC thermodynamic characteristics of starches, a blend of potato and corn starch was found to be easy to cook and form a gel but a 1ittle more resistant than the other starches in terms of retrogradation. Lightness (L) and yellowness (b) of Kwa-pyun increased while its redness (a) decreased by the storage. The color became whiter and the clarity decrease regardness of the kinds of starch, which became obvious after 3 days of storage. The hardness, springiness, adhesiveness and cohesiveness tended to decrease with storage, which was weak in a blend starch. The overall acceptability of Kwa-pyun made with a blend of potato and corn starch was evaluated as being the best among the samples tested. Therefore, for traditional kwa-pyun manufacture mungbean starch might be replaced with a blend of potato and corn starch which is less expensive and easily available in the market nowadays.

麻黃의 사람 비점막 섬유아세포 monocyte chemotactic protein 중 MCP - 1 , MCP - 2 , 및 MCP - 3 분비에 대한 효과

김현미,이향숙,김정선,조정제,유영천,안현종,최훈,임강현 대한본초학회 2002 大韓本草學會誌 Vol.17 No.1

Recent reports have proposed that Ephedrae Herba may modulate the immune response on allergy or asthma. Human nasal mucosal fibroblasts are a rich source cytokines, inflammatory mediators, and chemokines. Chemokines are important for the recruitment of leukocytes to sites of infection, which is essential in host defense. Objectives : The objective of this study was to investigate the effect of Ephedrae Herba on the release of the chemokines such as monocyte chemotactic protein(MCP)-1, MCP-2, and MCP-3 in human nasal mucosal fibroblasts after stimulation with cytokines like tumor necrosis factor -α(TNF-α), interferon-γ(IFN-γ), and interleukin -1β(IL-1β).

장애인 복지에 대한 기업의 참여

손경숙,장미녀 三陟大學校 産業科學技術硏究所 2003 産業科學技術硏究論文集 Vol.8 No.2

Such as Industrial accident by industrialization has created many kinds of problems, car accidents. chug abuse, environmental pollution, and hard to treat new disease that are constantly occurring. these can make the disable population is increasing gradually, so as this happens me need to find alternatives to meet their needs. Also, disabled people's unification and equality of opportunity are requiring more attention because the social environmental change has become more complex due to various and changes. There needs to be a realization about disabled people. This study wishes to find a troubleshooting plan, by active participation of enterprises as well as national policy about welfare to solve many kinds of problems. The study plans to look into the present situation and actual condition about their welfare in order to present the directions that society can be help to them through the correct recognition and their actuality about them. The whole nation, including the government and enterprises, should be interested in problem about disabled people. They should try to establish and improve the welfare policy about them. Moreover. they should focus on the dignity and value of human and must investigate their essence. It is necessary to study for the true definition, many conditions, self-actualization about disabled people's welfare and the direction of welfare policy of disabled people. Social integration of disabled people should be solidified in the aspect that disabled people have the right and dignity about government as a members of society. Home. society. enterprises and governments must solve by common efforts the problems of disabled people. They have to realize a previous development and welfare about disabled people and propel the welfare service for disabled people synthetically and systematically in order not to experience social disadvantages due to a mental and physical disorder.