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In this study, we aimed to investigate the antioxidant and immunopotentiating effects of leaves of cherry elaeagnus (Elaeagnus multiflora, Em). The leaf, stem, and root of Em have been used in Kampo medicine. There are data on the therapeutic effects of Em fruit, but no information on its leaves. We thus investigated the antioxidant and immunopotentiating effects of Em leaf extract. Balb/c mouse spleen cells were treated with concanavalin A and hot aqueous Em leaf extract (50, 100, and 200 μg/mL), and the effects on spleen cell proliferation and the secretion of interleukin (IL)-2, IL-4, and IL-10 were evaluated. A concentration-dependent increase in the secretion of IL-2 and IL-4 cytokines was observed, with concentrations of 1.86 pg/mL of IL-2 and 37.63 pg/mL of IL-4 when cells were treated with 200 ㎍/mL of the extract. Natural killer (NK) cell activity was determined based on a co-culture of spleen cells and Yac-1 cells. NK activity gradually increased in a concentration-dependent manne. Further, 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity was 58.5% and 2,2ʹ-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity was 78.2% with an extract concentration of 200 ㎍/mL. The high antioxidant activity of the hot water extract of Em leaves suggests their potential therapeutic value and warrants further studies.
김미리 ( Mi Ri Kim ), 차미란 ( Mi Ran Cha ), 최연희 ( Yeon Hee Choi ), 최춘환 ( Chun Whan Choi ), 최상운 ( Sang Un Choi ), 김영섭 ( Young Sup Kim ), 김영균 ( Young Kyoon Kim ), 김영호 ( Young Ho Kim ), 유시용 ( Shi Yong Ryu ) 한국생약학회 2010 생약학회지 Vol.41 No.2
Extensive phytochemical investigation of the methanol extract from the whole plant of Youngia japonica (Asteraceae) led us to the isolation of a new guaiane-type sesquiterpene (1), together with three related guaianolides, youngiajaponicoside A (2), crepiside H (3) and crepeside E (4). The chemical structure of 1 was elucidated by the aid of spectroscopic analyses including 2D-NMR experiments (COSY, HMBC, HMQC and ROESY). The isolated components (1-4) were evaluated for the inhibitory effect on the proliferation of four cultured human tumor cell lines such as A549, SK-OV-3, SK-MEL-2 and HCT-15, in vitro.
이형숙(Lee, Hyeong Suk), 박해나(Park, Haena), 김건희(Kim, Keun Hee), 김미리(Kim, Mi Ri), 김민지(Kim, Mingee), 김빛나(Kim, Bit Na), 김은경(Kim, Eun Kyung), 김은영(Kim, Eun Yong), 김진희(Kim, Jin Hee), 성혜진(Seong, Hye Jin), 송미선(Song, Misun) 한국보건간호학회 2015 韓國保健看護學會誌 Vol.29 No.3
Purpose: This study was conducted to describe the knowledge and attitudes of nursing and non-nursing college students toward HIV/AIDS and Hepatitis B. Methods: A descriptive design was used for this study. The subjects were 123 nursing college students and 128 non-nursing college students. Self-administered questionnaires were used for data collection. Descriptive statistics, χ²-test, t-test, ANOVA, and Pearson correlation coefficient were used for data analysis. Results: Nursing students showed a greater understanding and more favorable attitudes towards HIV/AIDS and hepatitis B than non-nursing college students. Most non-nursing college students learned about HIV/AIDS and hepatitis B from Mass Media. In contrast, many nursing college students learned about HIV/AIDS and hepatitis B from technical books and lecture. Students who learned from technical books and lecture showed a higher score for knowledge and more favorable attitudes regarding HIV/AIDS and hepatitis B. Conclusion: The findings of this study demonstrated that educational opportunities in school are crucial to enhancing knowledge and attitudes toward HIV/AIDS and Hepatitis B. A structured education program using strategies to improve attitudes is necessary for college students.
Stimuli-triggered Formation of Polymersomes from W/O/W Multiple Double Emulsion Droplets Containing Poly(styrene)-<i>block</i>-poly(<i>N</i>-isopropylacrylamide-<i>co</i>-spironaphthoxazine methacryloyl)
<P>We report stimuli-triggered fabrication of polymersomes from water-in-oil-in-water (W/O/W) multiple double emulsion droplets and the dual-stimuli (temperature and UV) responsive behavior of corresponding polymersomes. The polymersome comprises Tween20, cholesterol; and poly(styrene)block-poly(N-isopropylacrylamide-co-spironaphthoxazine methacryloyl), i.e., PS-b-P(NIPAAm-co-SPO), synthesized by stepwise reversible addition fragmentation chain transfer (RAFT) polymerization. Amphiphilic PS-b-P(NIPAAm-co-SPO) copolymer forms micelles in water above the critical micelle concentration (CMC) of 0.7 g/L at 23 degrees C. The micelles show a temperature-driven aggregation among the micelles above 30.6 degrees C, confirmed by a decrease in UV-vis transmittance. The micelles also show a color change without colloidal instability under 365 urn UV at room temperature. PS-b-P(NIPAAm-co-SPO) plays not only a role of the polymeric surfactant in the preparation of W/O/W multiple double emulsions but also an important role in the stimuli-triggered transformation from multi- to single-core double emulsion droplets under heat and UV light irradiation. It was found that the morphological transformation of W/O/W multiple double. emulsions by UV irradiation was Much faster than temperature change. Dual-responsive polymersomes were simply prepared after solvent removal and they exhibit stable and reversible size and color variations under temperature and UV visible changes, respectively.</P>