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돈사폐수를 이용한 Polyhydroxyalkanoates의 생산
조경숙,박창호,류희욱 이화여자대학교 환경문제연구소 1997 이화환경연구 Vol.1 No.-
돈사폐수를 발효기질로 이용한 생분해성 고분자, polyhydroxyalkanoates(PHA) 의 생산이 가능하였다. 균주로는 질소 혹은 인 등의 비탄소 영양원의 제한 없이도 PHA 의 생산이 가능한 Azotobacter vinelandii UWD 를 이용하였고 돈사폐수배지에서의 PHA 생산특성을 glucose 합성배지에서의 특성과 비교하였다. A. vinelandii UWD 균주는 탄소원으로 glucose와 alkanoates 가 함유된 배지에서 alkanoates를 우선적으로 소비하여 주로 생장을 하고, glucose소비단계에서는 생장보다는 PHA를 생산하는 diauxic growth 를 하였다. 돈사폐수 원액을 배지로 사용할 경우 UWD 균주의 건조균체량과 PHA 함량이 낮았는데(0.91g/L, 37wt%), 이는 돈사폐수 중에 함유되어 있는 acetate, propionate, butyrate 및 valerate등과 같은 높은 농도의 alkanoates가 세포생장을 저해하기 때문이었다. 증류수로 50% 희석한 배지(총 생산 균체 건중량 2.02g/L)를 사용하는 것이 바람직한 것으로 밝혀졌다. 50% 희석돈사폐수 배지에 glucose를 3% 첨가하면 건조균체량은 9.40g/L로 현저하게 증가하였으며, 균체내 PHA 함량은 58.3%, PHA 생산속도는 0.11g/(L·h)이었다. Glucose 합성배지에서 합성된 PHA는 poly-β-hydroxybutyrate(PHB)의 형태이었고, 돈사폐수 배지로부터는 copolymer인 poly-β-hydroxybutyrate-co-β-hydroxyvalerate, P(HB-co-HV)를 2-8mol%합성할 수 있었다. 돈사폐수배지를 이용하여 PHA를 생산하는 동안 배지의 염도를 대략 50% 정도 제거가 가능하였다. Biodegradable polymer, polyhydroxyalkanoates(PHA), could be produced using swine wastewater (SW) as a fermentation substance. Azotobacter vinelandii UWD was able to produce PHA without limiting non-carbon nutrients such as nitrogen and phosphorous. Production characteristics of strain UWD in SW medium were compared with those in glucose synthetic medium. In the synthetic medium containing glucose and alkanoates as carbon sources. A. vinelandii UWD grew diauxically, first by using alkanoates and then by using glucose to produce biomass PHA. Using raw SW medium, cell mass production was small (0.91g/ L) and PHA content was 37wt% because cell growth was inhibited by alkanoates such as acetate, propionate, butyrate, and valerate contained in SW. Use of two-fold diluted SW as fermentation medium was desirable for PHA production. When 3% of glucose was added to the two-fold diluted SW, dry cell mass increased to 9.40g/L, and the PHA content and PHA production rate were 58.3wt% and 0.11g(L·h), respectively, When grown in SW media A. vinelandii UWD formed PHA copolymers containing β-hydroxybutyrate and β-hydroxyvalerate (HV), and HV content in the copolymer was 2-8mol% whereas homopolymer of poly-β-hydroxybutyrate was produced in the glucose synthetic medium. Approximately, 50% of salinity in the medium was reduced after PHA production using SW medium.
Goldman's Algorithm을 이용한 비외상성 흉통 환자의 분석
조석진,유진현,김찬웅,어은경,정구영 대한응급의학회 1999 대한응급의학회지 Vol.10 No.4
Back ground : It is important to evaluate the patient presenting with non-traumatic chest pain in emergency department, and it is also difficult to classify appropriately. The purpose of this study is to predict probability of acute myocardial infarction(AMI) and assess the group for low probability of AMI that is target for observation-unit of chest pain in emergency department. Methods : A prospective, clinical study was performed from March 1998 to August 1998 at the department of emergency medicine, Ewha Womans University Mokdong hospital. We classified high-probability group and low-probability group of AMI according to Goldman's algorithm and then compared with the final diagnosis. Results : The study included 218 patients. 84 patients(39%) was the high-probability group and 134 patients(61%) was the low-probability group of AMI. As compared with final diagnosis, AMI was 47 patients(56%) among the high-probability group and 1 patient(0.7%) among the low-probability group. We observed sensitivity of 98%, specificity of 78% in predicting AMI. Conclusion : The classification according to Goldman's algorithm may be useful predictor of AMI and improve triage for emergency department patients with chest pain. It is also helpful for management of observation-unit of chest pain in emergency department.
종합병원 입원환자의 알코올중독 유병율과 주치의의 인지도 조사
조석군,김경빈,장환일 大韓神經精神醫學會 1993 신경정신의학 Vol.32 No.6
The authors studied prevalence and physician's detection rate of alcoholism in 478 adult patients admitted at Kyung-Hee Medical Center from June to July in 1992. In this study, the authors used self-report questionnaire consisting of Korean version of DSM-Ⅲ-R, MAST, NAST(I)(Alcoholism Screening Test of Seoul National Mental Hospital(I)), and additional demographic questions. The results were as following : 1) Among 478 patients, prevalence of alcoholism was 26.8%(76 patients) in male and 1.6%(e patients) in female. 2) Among the patients who are identified as alcoholism by the authors, detection rates of alcoholism by the charge physicians were 24.2% in internal medicine and none in other departments. 3) Compared to the alcoholic patients who are not detected by the charge physicians, the alcoholic patients who are detected are more aged patients showing high scored MAST significantly and having liver diseases.
진행위암의 수술전 병기결정에서 문맥기 나선식 CT의 유용성 : Usefulness of Helical CT during the Portal Venous Phase
조준식,신경숙 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.2
The purpose of this study was to evaluate the effect of helical CT during the portal venous phase after oral ingestion of water in the preoperative staging of advanced gastric carcinomas. Of the patients underwent helical CT scanning for preoperative staging of gastric carcinoma, 70 patients with pathologically proved advanced gastric carcinomas were selected in this study. The patients ingested 600-800m1 of water as a oral contrast agent before CT scanning. A total 130150ml of nonionic contrast material was administered at the rate of 4ml/s. We obtained portal venous phase images at 60 s and equilibrium phase images at 3 min after the start of bolus administration of intravenous contrast material. Two-phase CT findings were prospectively evaluated and correlated with histopathologic findings according to TNM classification. The primary tumors were detected on CT scans in 69 (98.5%) of 70 cases. The accuracy of CT for T staging was 74.3% (52 of 70 cases). T staging was understaged in 13 cases (18.6%) and overstaged in five (7.1%). The accuracy for N staging was 67.1% (47 of 70 cases). N staging was understaged in 16 cases (22.9%) and overstaged in seven (10%). Our results suggest that helical CT during the portal venous phase can improve the accuracy of CT in the preoperative staging of advanced gastric carcinomas.
趙誠旭,車敬石 대한치과교정학회 1992 대한치과교정학회지 Vol.22 No.1
To find out the differences between periodontal ligament cells (PDL cells) and gingival fibroblast cells (GFB cells), alkaline phosphatase, a marker enzyme for osteoblast, was used to measure the activities and 45CaCl₂isotope was used to find out cellular and release of 45Ca, a requisite for bone formation, PDL cells and GFB cells from 1 to 5 passages were also measured in alkaline phosphatase activity assay. By the use of above methods, followings were concluded that the PDL cells and the GFB cells have characteristics that are different from each other. In that PDL cells showed large amount of calcium uptake ad large amount of calcium release in initial stage, they seem to possess characteristics which are similar to osteoblast-like cells. 1. The PDL cells, in contrast to the gingival fibroblast, showed exceedingly high alkaline phosphatase activity which was highest at the second passage, decreasing thereon. But gingival fibroblasts cells showed no distinct differences in alkaline phosphatase activity as the passage were elapsed. 2. For both PDL cells and GF cells, the 45Ca uptake was greatest at 2 hours period. The PDL cells showed higher measuring than GFB cells through out the whole time period. 3. Whereas the GFB cells showed slow increase of 45Ca release as time relapsed, the PDL cells showed rapid increase of 45Ca release.
위에 생긴 이소성 췌장의 진단에 있어 경구 조영제로 물을 사용한 후 조영증강 역동적 CT의 가치
조준식,신경숙 충남대학교 의과대학 지역사회의학연구소 1997 충남의대잡지 Vol.24 No.2
To describe the usual and unusual CT findings of heterotopic pancreatic of the stomach and to evaluate the usefulness of dynamic contrast-enhanced CT with water as an oral contrast agent in diagnosing heterotopic pancreas We retrospectively reviewed CT findings in 10 patients with pathologically proved gastric heterotopic pancreas by operation (n=9) or endoscopic resection (n=1). In three patients, conventional CT with diluted Gastrografin solution as an oral contrast agent was performed. In seven patients, two-phase dynamic CT scans after ingestion of 600-800 ml of water were obtained in the early phase and equilibrium phase. Dynamic CT findings of heterotopic pancreas were correlated with histopathologic findings. All 10 heterotopic pancreatic rests were detected with conventional CT or dynamic CT. In seven cases who underwent dynamic CT after ingestion of water, highly enhanced mucosal layers overlying the lesions were identified and all lesions were well delineated in the submucosal layer In four of these seven cases, heterotopic pancreas showed high enhancement similar to that of adjacent normal pancreatic enhancement in the early phase of dynamic CT. The usual CT findings of gastric heterotopic pancreas were oval-shaped submucosal masses (<3cm) in the gastric antrum. The unusual CT findings were infiltrating heterotopic pancreas into the muscle layer in one, cystic dilatation of heterotopic pancreatic ducts in two, and malignant change with mucin secreting cystadencoarcinoma in one. Gastric heterotopic pancreas usually showed oval-shaped submucosal masses (<3cm) in the gastric antrum with high enhancement similar the that of pancreatic enhancment in the early phase of dynamic CT. Our results suggest that contrast-enhanced dynamic CT after ingestion of water may be helpful in diagnosing gastric heterotopic pancreas.
돈사폐수를 이용한 Polyhydroxyalkanoates의 생산
조경숙,류희욱,박창호 이화여자대학교 생명과학연구소 1996 생명과학연구논문집 Vol.7 No.-
돈사페수를 발효기질로 이용한 생분해성 고분자, polyhydroxyalkanoates(PHA)의 생산이 가능하였다. 균주로는 질소 혹은 인 등의 비탄소 영양원의 제한 없이도 PHA의 생산이 가능한 Azotobacter vinelandii UWD를 이용하였고 돈사폐수배지에서의 PHA 생산특성을 glucose 합성배지에서의 특성과 비교하였다. A. vinelandii uwd균주는 탄소원으로 glucose와 alkanoates가 함유된 배지에서 alkanoates를 우선적으로 소비하여 주로 생장을 하고, glucose 소비단계에서는 생장보다는 PHA를 생산하는 diauxic growth를 하였다. 돈사폐수 원액을 배지로 사용할 경우 UWD 균주의 건조균체량과 PHA함량이 낮았는데(0.91 g/L,37 wt%). 이는 돈사폐수 중에 함유되어 있는 acetate, propionate, butyrate 및 valerate 등과 같은 높은 농도의 alkanoates가 세포생장을 저해하기 때문이었다. 증류수로 50% 희석한 배지(총 생산 균체 건중량 2.02g/L)를 사용하는 것이 바람직한 것으로 밝혀졌다. 50% 희석 돈사폐수 배지에 glucose를 3% 첨가하면 건조균체량은 9.40g/L로 현저하게 증가하였으며, 균체내 PHA 함량은 58.3%, PHA 생산속도는 0.11g/(L·h)이었다. Glucose 합성배지에서 합성된 PHA는 poly-β-hydroxybutyrate(PHB)의 형태이었고, 돈사폐수 배지로부터는 copolymer인 poly-β-hydroxybutyrate-co-β-hydroxyvalerate, P(HB-co-HV)를 2-8mol% 합성할 수 있었다. 돈사폐수배지를 이용하여 PHA를 생산하는 동안 배지의 염도를 대략 50% 정도 제거가 가능하였다. Biodegradable polymer, polyhydroxyalkanoates(PHA), could be produced using swine wastewater (SW)as a fermentation substance. Azotobacter vinelandii UWD was able to produce PHA without limiting non-carbon nutrients such as nitrogen and phosphorous. Production characteristics of strain UWD in SW medium were compared with those in glucose sythetic medium In the synthetic medium containing glucose and alkanoates as carbon sources. A vinelandii UWD grew diauxically, first by using alkanoates and then by using glucose to produce biomass and PHA. Using raw SW medium, cell mass production was small(0.91 g/L)and PHA content was 37 wt% because cell growth was inhibited by alkanoates such as acetate, propionate, butyrate, and valerate contained in SW. Use of two-fold diluted SW as fermentation medium was desirable for PHA production. When 3% of glucose was added to the two-fold diluted SW, dry cell mass increased to 9.40 g/L, and the PHA content and PHA production rate were 58.3 wt% and 0.11g/(L·h), respectively. When grown in SW media A. vinelandii UWD formed PHA copolymers containing β-hydroxybutyrate and β-hydroxyvalerate(HV), and HV content in the copolymer was 2-8mol% whereas homopolymer of poly-β-hydroxybutyrate was produced in the glucose synthetic medium. Approximately 50% of salinity in the medium was reduced after PHA production using SW medium.
청가시덩굴(Smilax sieboldii Miq.)의 잎 및 지하부의 성분과 항고지혈작용에 관한 연구
조경열,우미희,오인숙 대구효성가톨릭대학교 응용과학연구소 1991 응용과학연구논문집 Vol.1 No.-
Nutritional value of the leaf and antihyperlipidemic effect of the subterranean part of Smilax siebolidd Miq.(Liliaceae) were investigated in this experiment. 8 minerals, 17 amino acids and 5 fatty acids were quantitatively analyzed in the leaf. In comparison with other vegetables, the content of amino acids was about twice as much as those and a lot of essential amino acids were found. The content of fatty acids was similar with that of other vegetables. In addition, various trace elements such as Mg, Zn and Mn were contained. The chloroform extract of the subterranean part of this plant was effective to decrease total cholesterol and triglyceride level and decrease HDL-cholesterol level in plasma of mouse.
부식된 하수관에서 분리된 곰팡이 OMSOf1의 황화수소 제거 특성
조경숙,박상진 이화여자대학교 환경문제연구소 1997 이화환경연구 Vol.1 No.-
심하게 부식된 콘크리트 하수관으로부터 황산화 곰팡이, OMSOf1을 분리하였다. 분리한 곰팡이의 성장 특성, 포자 형성 방법 및 포자의 크기와 모양을 조사한 결과, Sporormia속에 속하는 것으로 사료되었다. 곰팡이 OMSOf1은 균체내의 황화물 산화효소계에 의해 H₂S와 같은 황화물을 thiosulfate로 산화하였다. 고흡수성 수지(sodium polyacrylic acid) 와 polyurethane을 혼합하여 만든 담체에 biofilter에 28ppm의 H₂S를 50ml/min의 유속으로 통기시킨 결과, 약 15일 동안은 외부로부터의 수분을 공급하지 않는 조건하에서도 거의 100%의 H₂S 제거효율이 저하되었을 때에는 담채에 수분을 공급해 줌으로써 황화수소의 제거효율이 회복되는 것으로 밝혀졌다. A sulfide-oxidizing fungus was isolated from corroded concrete sewer pipes. From the physiological and morphological properties such as growth characteristics on diverse media, reproduction pattern, and size and shape of spores, the isolated fungus was considered as Sporormia sp. The fungus OMSOf1 could oxidize hydrogen sulfide(H₂S)to thiosulfate due to intracellular enzyme systems. The fungus was immobilized in polyurethane carriers which contained super absorbent polymer(sodium polyacrylic acid) permeated with mineral medium, and polyurethane. When 28ppm of H₂S was supplied to the biofilter packed with the carriers at 50ml/min, more than 95% removal ratio of H₂S was obtained for 15 days without supply of water. The reduction of H₂S removal ratio by drying of the carriers could be recovered by supply of water.
曺東震,韓俊澤,劉憲一,朴龍鎭,金錫胤,慶在浩 弘益大學校 1974 弘大論叢 Vol.6 No.-
The matrix of ductile cast iron are welded by pre-heating it at 300℃ in the room temperature. Through the mechanical, non-desbructive testing and analysis of the composition. the possibility if arc welding of ductile cast iron are studied as follows; 1. L electrode is the best among them in weldability, impact value and machinability. The next is LB electrode followed by S electrode. C electrode is last. However, the tensile strength showed in the following order: LB, S, L, C. 2. LB, S, L, C, all are available for the welding of cast iron. 3. The above experiment showed that L and LB are good in general. In addition LB is low in price, we therefore prospect that it will be widely used as the electrode for cast iron.