직장 융모성 선종의 병리학적 검토

임경욱,신영도,이석환,이기형 대한대장항문학회 1997 Annals of Coloproctolgy Vol.13 No.2

Association of 20 potential ATP2B1‐interacting genes with blood pressure in Koreans

Kyung‐Won Hong,Hyun‐Seok Jin,Ji‐Eun Lim,Bermseok Oh 한국유전학회 2011 Genes & Genomics Vol.33 No.3

Plasma membrane calcium‐transporting ATPase 1 (ATP2B1)is associated significantly with blood pressure in Caucasians and Asians. ATP2B1 regulates calcium homeostasis and belongs to the P‐type calcium pump family; several studies have identified diverse proteins that bind to ATP2B1. We hypothesized that ATP2B1 regulates blood pressure through ATP2B1‐interacting genes. To this end, 20 potential ATP2B1‐interacting genes were selected, 197 SNPs of which were analyzed for their association of systolic and diastolic blood pressure. These 20 genes were categorized into 2 groups: ATP2B1‐binding genes and ATP2B1‐cleaving calpain family members. Three ATP2B1‐binding genes (CALM1, NOS1, and PDLIM1)were associated with blood pressure, and a SNP in CALM1(rs2401887) generated the strongest association signal (beta=‐3.60±0.92, p=8.9×10^(‐5) for systolic blood pressure and beta=‐1.40±0.62, p=0.02 for diastolic blood pressure). Of the calpain family members, 3 genes (CAPN6, CAPN9, and CAPN10)were associated with blood pressure, and the CAPN10 SNP rs4676348 yielded the strongest association signal (beta=‐0.88±0.27, p=0.001 for systolic blood pressure and beta=‐0.58±0.18, p=0.015 for diastolic blood pressure). Further, the interaction of CALM1 to ATP2B1 was examined using the blood pressure of individuals who carried both variants of CALM1and ATP2B1 genes. Similarly the interaction of CAPN10 to ATP2B1 was also examined. The CALM1 variant (rs2401887)and CAPN10 variants (rs4676348) appear to decrease blood pressure further in addition to the decrease by the variant (rs17249754) of ATP2B1, which suggests that ATP2B1 might regulate blood pressure through the ATP2B1‐interacting genes CALM1 and CAPN10.

熱環境의 理論的 背景에 관한 硏究

林晩澤,朴京甲,鄭圭福,鄭碩熙 조선대학교 국토개발연구소 1993 국토개발연구 Vol.13 No.1

The design ranges of human-environmental system can be divided into seven kinds, water, thermal, air, light, acoustic, color, psychology and environment. This study aims at clarifying the theoretical background of thermal environment is what point of view is historically. Thermal environment developed based on the thermal comfort index, standard and law establishment etc. To constitute the amenity of thermal environment and to control it, it needs to prediction and adaptability.

Genetic algorithm을 이용한 supply chain network에서의 최적생산 분배에 관한 연구

임석진,정석재,김경섭,박면웅 한국경영과학회 2003 한국경영과학회 학술대회논문집 Vol.- No.1(1)

Recently, a multi facility, multi product and multi period industrial problem has been widely investigated in Supply Chain Management (SCM). One of the key issues in the current SCM research area involves reducing both production and distribution costs. The purpose of this study is to determine the optimum quantity of production and transportation with minimum cost in the supply chain network. We have presented a mathematical model that deals with real world factors and constraints. Considering the complexity of solving such model, we have applied the genetic algorithm approach for solving this model computational experiments using a commercial genetic algorithm based optimizer. The results show that the real size problems we encountered can be solved in reasonable time.

최적형상의 고속용 엔드밀 설계를 위한 프로그램 개발

고성림,한창규,서천석,김경배 한국공작기계학회 2003 한국공작기계학회 춘계학술대회논문집 Vol.2003 No.-

The tool geometry parameters and cutting process have complex relationships. Until now, various cutting test were needed to acquire optimal design of end mill for the purpose of high speed machining, due to the insufficient knowledge about cutting process in high speed machining. Using various tools with differentgeometry, relationships between tool geometry parameter (rake angle, clearance angle, length of cutter) and cutting process (cutting force, surface accuracy, surface roughness) have been studied. Acquired data can be used to design optimal tool for high speed machining

골격근 근형질세망의 ATPase활성도에 대한 Vanillylnonanamide의 영향

박경섭,홍장희,류영수,성지연,허강민,임종호,이재흔,석정호 충남대학교 의과대학 지역사회의학연구소 1999 충남의대잡지 Vol.26 No.2

To investigate the effect of vanillylnonanamide(VN) on the ATPase activity of the sarcoplasmic reticulum(SR) of the skeletal muscle, we prepared the SR vesicles from the back muscle of the rabbit, and measured ATPase activity. The results as follows: Ca-ATPase activity was about 50% in the total ATPase activity of skeletal muscular SR. In the reaction mixture with calcium, 100μM VN increased ATPase activity to 20%, and 100 nM and 10 μM thapsigargin(THP) inhibited the ATPase activity to 50% and 60%, respectively. And 100 μM VN plus 100 nM or 10 μM THP more inhibited ATPase activity than THP alone did it. However, without calcium, 100μM VN did not affect ATPase activity, and 10 μM THP inhibited it to 41%, but VN plus THP inhibited it to 31%. The above results show that VN in the reaction mixture with or without calcium has the different action to ATPase activity when it is used alone or together with Ca-ATPase inhibitor THP. This suggests that VN might increase or decrease the skeletal SR Ca-ATPase activity through affecting the lipid membrane around the Ca-ATPase.

상부 조기 위암 환자의 복강경하 상부 위절제술 3례 경험

조규석,김형철,박경규,이문수,송옥평,임철완,신웅진,주종우,유기원 순천향의학연구소;Soonchunhyang Medical Research Institute 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

Background : The reported incidence of early gastric cancer located in the upper portion of the stomach has been increasing with the recent advances in its diagnosis and screening. Recently, we have successfully performed laparoscopic assisted proximal gastrectomy and gastric tube reconstruction without pyloroplasty on three patients with early gastric carcinoma localized to the upper third of the stomach. We describe our modification of this procedure in this report. Method : After creating an surgical pneumoperitoneum, the stomach was mobilized using laparoscopic coagulating shears. Upper half of the greater curvature and three-quarters of the lesser curvature were then dissected along with regoinal D2 lymphadenectomy. This was followed by a 5 cm, longitudinal mini-laparotomy in the upper abdomen, and the construction of the exteriorized stomach with a gastric tube measuring 20 cm long and 4 cm wide. Reconstruction with an esophagogastrostomy was performed using a circular stapler. Result : No post-operative morbidity or mortality was observed in this small series of patients. The average operative time was 250 minutes (range 220-300 minutes), and the average blood loss was 150 ml (range 90-180 ml). The mean number of lymph nodes harvested during these laparoscopic proximal gastrectomies was 24 nodes (range 22-25 lymph nodes). The average postoperative hospital stays was 7.5 days (range 7-8 days). Conclusion : Our technique of laparoscopic assisted proximal gastrectomy and gastric tube reconstruction without phloroplasty offers a minimally invasive technique with the potential of impoving the post-operative quality of life patients with an early-stage proximal gastric cancer.

RAW264.7 세포에서 interferon-r 및 LPS에 의해 유도되는 NO생성에 미치는 TALT-35의 영향

박종일,박경석,김종석,박지훈,윤은진,송경섭,서강식,김훈,윤완희,박승길,임규,황병두 충남대학교 생물공학연구소 2006 생물공학연구지 Vol.12 No.-

TALP-35 purified from human term placenta is known to increase microtubule polymerization and stabilize the polymerized microtubule. To examine the effect of TALP-35 on immune system this study was performed. MTT assay was performed to investigate the effect of TALP-35 on the proliferation of RAW264.7 cells. TALP-35 dose dependently suppress the proliferation of RAW264.7 cells at high concentration (above 1 μM) in unstimulated cells, in case of 10 μM TALP-35 treated cells the suppression was 25% but in stimulated cells it was only 15%. Cosedimentation assay was carried out to investigate whether TALP-35 can bind to tubulin of RAW264.7, monocyte/macrophage lineage of mouse, and polymerize it. TALP-35 polymerize the tubulin of RAW264.7 cells and sedimented in dose-dependent manner. To investigate the effect of TALP-35 on the expression of iNOS protein western blotting was performed. The expression level of iNOS was decreased dose dependently in high concentration of TALP-35 treatment. To examine the activity of iNOS, secreted NO was determined by method based on Griess reaction. Interferon-γ and LPS-stimulated production of NO from RAW264.7 cells was decreased dose dependently above 0.1 μM concentration of TALP-35 and 50% is decreased at 10μM of it. This study shows TALP-35 can control cytokine induced-iNOS expression therefore it might control inflammatory diseases.

악안면 골결손부의 이종골이식을 이용한 수복에 관한 연구

김경원,조용석,이경호,임현범 충북대학교 의과대학 충북대학교 의학연구소 1998 忠北醫大學術誌 Vol.8 No.2

연구목적 : 구강 및 악안면영역의 소수술시 발생할 수 있는 골결손부에 대하여 이종골을 이용한 골이식을 시행하고 치료결과 및 예후를 평가하고자 하였다. 재료 및 방법 : 1996년 1월부터 1996년 12월까지 내원한 환자중 골결손부에 대힌 골이식이 필요하였던 환자중 이식 후 추적 관찰이 가능하였던 36명에 대하여 의무 기록과 방사선 사진을 이용하여 분석하였다 결과 : 대상 환자중 10대와 20대(72.7%)가 가장 많았으며, 골이식이 필요하였던 질병으로는 낭종, 양성 종양, 매복치, 치근단 질환 등이었으며, 이식후 평균 7개월의 추적 관찰을 시행한 결과 36례중 6례(약17%)에서 술후 경미한 정도의 감염 소견이 있었으며 그 외는 특별한 합병증이 발견되지 않았다. 결론 : 구강 및 악안면영역의 비교적 작은 골결손부에 대하여 이종골이식을 선택적으로 시행할 수 있을 것으로 생각된다. Purpose : We evaluated the result and prognosis of the heterogenic bone graft for restoration of the bone defect in oral and maxillofacial region. Materials and Methods : We collected and analysed the patents who received the heteogenic bone graft from January 1996 to December 1996 in Chungbuk National University Hospital. Results : Prevalent age groups of heterogenic bone graft were 2nd and 3rd decades(72.2%), and cysts and benign tumors, impacted teeth, apical lesions were causative diseases of bone defects. The average period of follow-up was 7 months after operation. Major complications were postoperative infections(17%), but they were not severe. Conclusion : We recommend a heterogenic bone graft for restoration of the relatively small bone defect in oral and maxillofacial region selectively.

오렌지로부터 아플라톡신B₁의 정량분석에 관한 연구

장향동,백광균,구경림,옥선미,최진석,고은주,류성렬 한국공업화학회 2004 응용화학 Vol.8 No.2

Aflatoxin B₁ was known to have the strongest poisonous character among Aflatoxin compounds. To search the production of Aflatoxin B1 according to the change of temperature, relative humidity and incubation days, orange incubated with Aspergillus parasiticus KCCM 35078 was incubated at 20℃, 25℃, 30℃ at relative humidity 60%, 70%, 80% and for incubation time 3, 4, 5, 6, 7 days.. Production of Aflatoxin B₁ shows that the highest production appeared at 25℃ and its relative humidity 70%. The result that was quantitatively analyzed by using HPLC was 77.41ppm, and by using Fluoroscence detector was 87.87ppm. - Production of Aflatoxin B₁ shows that the lowest production appreared at 20℃ and its relative humidity 80%(HPLC), 60%(Fluoroscence detector). The result that was quantitatively analyzed by using HPLC was 4.47ppm, and by using Fluoroscence detector was 4.17ppm. The structure of Aflatoxin B₁ was identify with authentic sample by using instrumental analysis methods of GC/MS, ¹H-NMR, FT-IR respectively.