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Yim, Joung Han,Ahn, Se Hun,Kim, Sung Jin,Lee, Yoo Kyung,Park, Kyu Jin,Lee, Hong Kum Trans Tech Publications, Ltd. 2005 Key Engineering Materials Vol.277 No.-
<P>To find a novel exopolysaccharide, marine bacterial strains were isolated from coastal regions of Korea. Strain 00SS11568 was then selected as it produced a mucous exopolysaccharide during the stationary phase in a batch culture. The isolate was identified as Alteromonas sp. based on its 16S rDNA sequence, morphological, and biochemical properties. The exopolysaccharide, designated as p-11568, exhibited an emulsifying ability. The Emulsification Index (E24) of 0.1% p- 11568 was 77.4% with an emulsified kerosene content, and was higher than those of commercial polysaccharides, such as xanthan gum (26.1%), gellan gum (1.3%), and sodium alginate (2.0%). p- 11568 was found to be composed of glucose and galactose as the main natural sugars in a molar ratio of 1.3:1, along with uronic acid (18.9%, w/w) and sulfate groups (1.2% w/w). The average molecular mass was 4.4 x 105 daltons by gel filtration chromatography. The effects of pH, temperature, inorganic compounds, and C and N sources were tested to obtain the optimal medium composition for the production of p-11568. Under optimal growth conditions with the M-11568 medium, 14.9 g of crude p-11568 per liter was obtained.</P>
Industrial Applications of Biopolymer from Marine Microorganisms
Joung Han Yim 한국당과학회 2009 한국당과학회 학술대회 Vol.2009 No.1
Many marine microorganisms produce biopolymers, and some polymers are economical materials in industry. From their wide diversities in structure and physical properties, we have investigated useful biopolymer. The sulfated exopolysaccharide p-KG03, which is produced by the marine microalga Gyrodinium impudicum strain KG03, exhibited impressive antiviral activity in vitro (EC50 = 26.9 μg/ml) against the encephalomyocarditis virus (EMCV), immunostimulating activity by NK cell activation, and bioflocculant activity for the industrial application. P-21653 (Antarctic marine bacterium Pseudoalteromonas arctica KOPRI 21653) is useful cryoprotective agent of red blood cell and animal cell, and also use for enzyme stabilizer. P-m10356 (marine bacterium Hahella chejuensis strain 96CJ10356) is potential release-control matrix for the oral deliverly system and UV-protective agent. And P-11568 (marine bacterium Alteromonas sp. KCTC10324) is excellent emulsifier. Therefore we introduce some results about diversity of marine biopolymer and its applications.
Joung Han Yim,Hong Kum Lee 한국미생물학회 2004 The journal of microbiology Vol.42 No.4
An exopolysaccharide-producing microalgal dinoflagellate was isolated from a red-tide bloom and designated strain KG03. A bacteria-free culture of strain KG03 was achieved using a modified wash with phototaxis and antibiotic treatment. Combined treatment with neomycin and cephalosporin was the most effective for eliminating the bacteria associated with the microalgae. Strain KG03 was identified as Gyrodinium impudicum by analyzing the ITS regions of the 5.8S rDNA, 18S rDNA, morphological phenotype and fatty acid composition. The exopolysaccharide production and cell growth in a 300-ml photobioreactor were increased 2.7- and 2.4-fold, respectively, compared with that in a flask culture at the first isolation step.