http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Porphyromonas gingivalis exacerbates the progression of fatty liver disease via CD36-PPARγ pathway
( Ji-su Ahn ),( Ji Won Yang ),( Su-jeong Oh ),( Ye Young Shin ),( Min-jung Kang ),( Hae Ryoun Park ),( Yoojin Seo ),( Hyung-sik Kim ) 생화학분자생물학회(구 한국생화학분자생물학회) 2021 BMB Reports Vol.54 No.6
Periodontal diseases have been reported to have a multidirectional association with metabolic disorders. We sought to investigate the correlation between periodontitis and diabetes or fatty liver disease using HFD-fed obese mice inoculated with P. gingivalis. Body weight, alveolar bone loss, serological biochemistry, and glucose level were determined to evaluate the pathophysiology of periodontitis and diabetes. For the evaluation of fatty liver disease, hepatic nonalcoholic steatohepatitis (NASH) was assessed by scoring steatosis, inflammation, hepatocyte ballooning and the crucial signaling pathways involved in liver metabolism were analyzed. The C-reactive protein (CRP) level and NASH score in P. gingivalis-infected obese mice were significantly elevated. Particularly, the extensive lobular inflammation was observed in the liver of obese mice infected with P. gingivalis. Moreover, the expression of metabolic regulatory factors, including peroxisome proliferator-activated receptor γ (Pparγ) and the fatty acid transporter Cd36, was up-regulated in the liver of P. gingivalis-infected obese mice. However, inoculation of P. gingivalis had no significant influence on glucose homeostasis, insulin resistance, and hepatic mTOR/AMPK signaling. In conclusion, our results indicate that P. gingivalis can induce the progression of fatty liver disease in HFD-fed mice through the upregulation of CD36-PPARγ axis. [BMB Reports 2021; 54(6): 323-328]
Ye Ji Ahn,Wan Su Yun,최진실,김우철,Su Hoon Lee,Dong Jun Park,박정은,Jaehong Key,서영준 대한의용생체공학회 2021 Biomedical Engineering Letters (BMEL) Vol.11 No.1
Recently, application of stem cell therapy in regenerative medicine has become an active field of study. Mesenchymal stem cells (MSCs) are known to have a strong ability for homing. MSCs labeled with superparamagnetic iron oxide nanoparticles (SPIONs) exhibit enhanced homing due to magnetic attraction. We have designed a SPION that has a cluster core of iron oxide-based nanoparticles coated with PLGA-Cy5.5. We optimized the nanoparticles for internalization to enable the transport of PCS nanoparticles through endocytosis into MSCs. The migration of magnetized MSCs with SPION by static magnets was seen in vitro. The auditory hair cells do not regenerate once damaged, ototoxic mouse model was generated by administration of kanamycin and furosemide. SPION labeled MSC’s were administered through different injection routes in the ototoxic animal model. As result, the intratympanic administration group with magnet had the highest number of cells in the brain followed by the liver, cochlea, and kidney as compared to those in the control groups. The synthesized PCS (poly clustered superparamagnetic iron oxide) nanoparticles, together with MSCs, by magnetic attraction, could synergistically enhance stem cell delivery.
A case of TBC1D32-related ciliopathy with novel compound heterozygous variants
Ahn, Ji Ye,Kim, Soo Yeon,Lim, Byung Chan,Kim, Ki Joong,Chae, Jong Hee Korean Society of Medical Genetics and Genomics 2021 대한의학유전학회지 Vol.18 No.1
Primary cilium has a signal transduction function that is essential for brain development, and also determines cell polarity and acts as a mediator for important signaling systems, especially the Sonic Hedgehog (SHH) pathway. TBC1D32 is a ciliary protein, implicated in SHH signaling. Biallelic mutations in the TBC1D32 gene causes a kind of ciliopathy, heterogeneous developmental or degenerative disorders that affect multiple organs, including the brain. Here we report a boy who carried compound heterozygous variants in TBC1D32. The patient showed hypotonia, respiratory difficulty, and multiple anomalies at his birth. He was diagnosed with congenital hypopituitarism and treated with T4, hydrocortisone, and growth hormone. Despite the hormonal replacement, the patient needed long-term respiratory support with tracheostomy and nutritional support with a feeding tube. His developmental milestones were severely retarded. Hydrocephalus and strabismus developed and both required surgery, during the outpatient follow-up. Whole-exome sequencing indicated compound heterozygous variants, c.2200C>T (p.Arg734<sup>*</sup>) and c.156-1G>T, in TBC1D32 gene. This is the first Korean case of TBC1D32-related ciliopathy and we reported detailed and sequential clinical features. This case demonstrated the utility of whole-exome sequencing and provided valuable clinical data on ultra-rare disease.
Ji Ye Ahn,Il Yong Kim,Sae Jin Oh,Hye Sook Hwang,Sun Shin Yi,Yo Na Kim,Jae Hoon Shin,Yeo Sung Yoon,Je Kyung Seong 한국실험동물학회 2014 Laboratory Animal Research Vol.30 No.2
Pig pancreas may be a therapeutic resource for human diabetic patients. However, this potential is hindered by a lack of knowledge of the molecular events of pig pancreas development. In this study, the embryonic day 60, neonate and 6-month protein profiles of pig pancreas were ascertained at using twodimensional gel electrophoresis and matrix assisted laser desorption/ionization-time of flight mass spectrometry. Twenty four proteins were differentially expressed during pig pancreas development. Among them, 12 spots increased and 7 spots decreased according to development. The expression of 5 protein were highest at birth. Expression of digestive enzymes including trypsin, pancreatic triacylglycerol lipase and pancreatic alpha-amylase was elevated in adults, whereas chymotrypsins were highly expressed in neonates. Proteins that were abundantly expressed during gestation were alpha-1-antitrypsin, alphafetoprotein and transferrins. Taken together, we found out that several proteins were significantly up- or down- regulated from pig pancreas based on developmental stage. This study will provide basis for understanding development of pig pancreas.
Variable Phenotypes of ZC4H2-Associated Rare Disease in Six Patients
Ji Ye Ahn,Soo Yeon Kim,Byung Chan Lim,Ki Joong Kim,Jong-Hee Chae 대한소아신경학회 2022 대한소아신경학회지 Vol.30 No.3
Purpose: Wieacker-Wolff syndrome is a rare disease caused by X-linked zinc finger C4H2-type containing (ZC4H2) mutations. It is characterized by arthrogryposis multiplex congenita (AMC) and intellectual disability (ID), including impairment of central and peripheral synaptic plasticity. Currently, it is named “ZC4H2-associated rare disease” (ZARD) due to various clinical features other than AMC and ID. Here, we report six cases of ZARD, and describe their variable clinical phenotypes. Methods: We analyzed the detailed clinical features and genotypes of six patients diagnosed by whole-exome sequencing or a chromosomal microarray. Results: In the four male patients, hemizygous mutations were found (c. 245A>C in two patients, c. 610C>A in one patient, and c.637C>T in one patient), and all variants were identified by Sanger sequencing. In the female patients, a 1.16-Mb deletion in Xq11.2, including ZC4H2, was identified by chromosomal microarray. All patients had heterogeneous phenotypes with variable severities. Motor delay was observed in all patients, four of whom could not walk independently. Other neurological features included ID, spasticity, and seizures. The craniofacial features included microcephaly, low-set ears, strabismus, ptosis, ocular motor apraxia, a U-shaped upper lip vermilion, short neck, and microretrognathia. The most common musculoskeletal symptoms were multiple arthrogryposis: metacarpophalangeal joint contracture, clubfoot, distal muscle weakness, Achilles tendon contracture, knee flexion contracture, camptodactyly, elbow flexion contracture, and hip subluxation. Conclusion: The ZARD phenotypes were prominent in male patients, and female patients with loss of function showed more severe symptoms. Further research is needed to clarify phenotypic variability in this rare disorder.
( Ye Ji Han ),( Eun Mi Chun ),( Chul Min Ahn ),( Jae Yeol Kim ),( Kwang Ha Yoo ),( Sang Haak Lee ),( Yu Il Kim ),( Ju Heon Park ),( Young Sik Park ),( Ji Ye Jung ) 대한결핵 및 호흡기학회 2016 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.121 No.-
Background: Smoking is strongly associated with severe illness causing burden to society`s health care system. As a representative of health institution, general hospital is responsible for promoting safe and healthy environment. The aim of study is to investigate smoking status, awareness and attitudes toward smoking cessation among hospital workers. Methods: This study was conducted on 949 employees working at tertiary hospital including healthcare providers and non-practitioner from April to May 2014. Results: 949 employees answered the questionnaire, and 14.6% of respondents had experience of smoking. (8.4% were current smokers.) In smoking group, majority were male (98.7%) and proportion of non-health care provider group (94.2%) was much higher with higher level of stress and low level of satisfaction toward their career. Those showed high frequency of attempt to stop smoking (83.5% multi-attempt), while the use of anti-smoking education (31.6%) and medical treatment was low (24.8%). Compared to non smokers, smokers presented more negative view on effectiveness of education and designation of smoke-free area in hospital. In multiple regression analysis, odd to respond positive to entire hospital being smoke-free following adjustment of gender, age, and working department was 3.82 for ex-smokers and 7.04 for non-smokers. Conclusions: Our study suggests smoking group among hospital employee with insufficient information have unfavorable attitudes toward smoking cessation, and this suggests need for further intervention to promote totally smoke-free hospital.