http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Jayasinghe, J.D.H.E.,Elvitigala, D.A.S.,Whang, I.,Nam, B.H.,Lee, J. Academic Press 2015 Fish & shellfish immunology Vol.45 No.2
Haptoglobin (Hp) and serum amyloid A (SAA) are two vital proteins involved in inflammatory reactions and are classified as acute-phase proteins. They are released from hepatocytes under inflammatory conditions to protect healthy cells from being damaged by pathogens or from self-destructive mechanisms. In this study, a previously constructed black rockfish (Sebastes schlegeli) cDNA library was used to identify the full-length cDNA sequences of Hp and SAA homologs (RfHp and RfSAA, respectively) and characterize them at the molecular level. As expected, in silico analysis of these homologs showed the typical domain architectures of their known counterparts. Open reading frames of RfHp and RfSAA consisted of 942-bp and 313-bp DNA sequences, respectively. The derived polypeptide sequence of RfHp was composed of 313 amino acids (aa) with a predicted molecular weight of 34 kD, whereas RfSAA had a 121-amino acid sequence with a molecular weight of 13 kD. Phylogenetic analysis as well as pairwise sequence alignment results showed that RfHp was more closely related to Oreochromis mossambicus from an evolutionary perspective while RfSAA was closely related to the Epinephelus coioides ortholog. Although both genes were expressed ubiquitously in the tissues analyzed, they were particularly expressed in liver tissue, suggesting their origin in hepatocytes. Quantitative real-time PCR analysis indicated that both RfHp and RfSAA were significantly up-regulated by both bacterial and viral stimulation in liver tissue, affirming their putative importance in the acute phase of first-line host immune defenses.
Vulnerability of roofing components to wind loads
Jayasinghe, N.C.,Ginger, J.D. Techno-Press 2011 Wind and Structures, An International Journal (WAS Vol.14 No.4
The vulnerability of roofing components of contemporary houses built in cyclonic regions of Australia is assessed for increasing wind speeds. The wind loads and the component strengths are treated as random variables with their probability distributions derived from available data, testing, structural analysis and experience. Design details including types of structural components of houses are obtained from surveying houses and analyzing engineering drawings. Wind load statistics on different areas of the roof are obtained by wind tunnel model studies and compared with Australian/New Zealand Standard, AS/NZS 1170.2. Reliability methods are used for calculating the vulnerability of roofing components independently over the roof. Cladding and batten fixings near the windward gable edge are found to experience larger negative pressures than prescribed in AS/NZS 1170.2, and are most vulnerable to failure.