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Ja Young Seol,Soon Young Choi,Nari Lee 대한암예방학회 2007 Journal of cancer prevention Vol.12 No.4
Leptin, the product of the obesity (ob) gene, is a multifunctional cytokine and a 16-kDa circulating hormone that primarily acts in the hypothalamus. It plays a key role in the regulation of food intake and energy expenditure. In addition, it has direct effects on many cell types on the periphery. Leptin acts through its transmembrane receptors (OB-Rb), which are the product of the leptin receptor (OB-R) gene. There are several alternative spliced isoforms (a-f). Only one of OB-R, which has the longest cytoplasmic domain, has full signaling capabilities and it is expressed in high levels in the hypothalamus. In the present study, we investigate the obligatory function of normal food intake and body weight regulation. In order to develop a cell model to screen the effects of bio-food materials regulating food intake and to research cancer, the leptin receptor (OB-Rb) gene from mouse brain total RNA was introduced into a retroviral vector (pLNCX2) designed for stably over-expressing OB-Rb. An ecotropic retroviral supernatant (pLNCX2-Ob-Rb) was added to 3T3-L1 (adipocyte cells) and HEK293 (human embryonic kidney cells). G418 was used as selection antibiotic for the establishment of cell lines that constitutively expressed the leptin receptor proteins. We assayed expression of the leptin receptor using western-blot and quantitative RT-PCR. (Cancer Prev Res 12, 240-245, 2007) Key Words: Leptin, Leptin receptor (OB-Rb), Stable cell line, Retroviral vector, Infection
Physical Analysis of nahQ tnpA Genes from Pseudomonas fluorescens
Seol, Ja-Young,Chol, Soon-Young,Min, Kyung-Hee The Microbiological Society of Korea 2001 The journal of microbiology Vol.39 No.4
Pseudomonas fluorescens SM11 is a naphthalene-degrading strain whose dissimilatory genes are cho-mosomally encoded. We have cloned the 2.9 kb Sal I fragment harboring genes for the naphthalene-degrading upper pathway. The nucleotide sequences were determined to be nahQ, napA, and partial regions of nahE genes. The nahQ encods a protein of 188 amino acid residues with a deduced molec-ular wight of 20.8kDa. The high homology with other proteins suggests that NAhQ may be an active and useful protein whtich gives as selective advantage to naphthalene degradatin. Transposase(TnpA)encodes a polypetide chain with a molecular mass of 41.8kDa consisting of 376 amino acid residues. The deduced anino acid sequence of tnpA revealed 96% idenitity with putative transposase of P. stutzeri OX1,. It was assumed that transposase plays an important role in the evloution of the catabloic-path way in the regulation of nah expression.
Seol, Ja young,Yoon, Ji Young,Jeong, Hee Sun,Joo, Nami,Choi, Soon Young Korean Society for Food Science of Animal Resource 2016 한국축산식품학회지 Vol.36 No.2
Many researchers revealed that collagen contribute to maintaining the skin’s elasticity and inhibit wrinkling of skin. Korean native cattle (Hanwoo) bone (leg bone, foot and tail) infusion contains the various inorganic materials, collagen and chondroitin sulfate. All of this, a large quantity of collagen is included in Hanwoo infusion. Therefore, this study emphasized on the effects of collagen in the Hanwoo bone infusion. For the first time, Hanwoo bone infusions were directly added to the media of Human Dermal Fibroblast (NHDF-c) to test anti-aging effects. First, it was identified that growth rate of skin fibroblast was increased. Furthermore, the Hanwoo bone infusion increased a 50% of fibroblast collagen synthesis. Also, suppression of skin fibroblast aging was confirmed by treatment Hanwoo bone infusion. In conclusion, this study demonstrates the effects of infusion made from Hanwoo leg bone, foot and tail on anti-aging, wrinkle inhibiting and skin fibroblast elasticity maintaining. Therefore, this study identified that traditional infusion has effects that are good for skin elasticity.
Immunomodulatory activity of sodium silicate containing selenium in LPS-stimulated immune cells
( Ja Seon Yoon ),( Joon Yeop Lee ),( Nak Young Baek ),( Bong Su Kang ),( Ji Yong Yun ),( Dang Yong Kim ),( Ja Kyoung Seol ),( Young Won Yun ),( Sang Yoon Nam ),( Jae Hwang Jeong ),( Jong Soo Kim ),( B 한국예방수의학회(구 한국수의공중보건학회) 2013 예방수의학회지 Vol.37 No.1
Selenium salts are toxic in large amounts, however, trace amounts are necessary for cellular function in many organisms, including all animals. Selenium is a component of the antioxidant enzymes glutathione peroxidase and thioredoxin reductase (which indirectly reduce certain oxidized molecules in animals and some plants), sodium silicate (Na2SiO3) possesses a number of biological activities, especially in improvement of cell growth. However, few studies on the effects of sodium silicate and selenium on an immune system have been reported so far. The objective of this experiment was to investigate the effect of soluble sodium silicate/Se on lipopolysaccharide (LPS)-induced inflammatory response in RAW264.7 cells. The production of nitric oxide (NO) and proinflammatory cytokines was examined in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. The cells were cultured in Dulbecco`s modified Eagle`s medium (DMEM). Concentration of 10 ppm or less of soluble sodium silicate/Se had no cytotoxic effect on RAW264.7 cells. The soluble sodium silicate/Se decreased LPS-induced production of NO but did not affect the levels of TNF-α, IL-2, and IFN-γ in RAW264.7 cells In addition, the sodium silicate/Se induced up regulation of IL-1β level in a does-dependent manner. These results suggest that the soluble sodium silicate/Se has a synergistic effect in down-regulation of inflammatory response in vitro.
Ja Young Seol,Ji Young Yoon,Hee Sun Jeong,Nami Joo,Soon Young Choi 한국축산식품학회 2016 한국축산식품학회지 Vol.36 No.2
Many researchers revealed that collagen contribute to maintaining the skin`s elasticity and inhibit wrinkling of skin. Korean native cattle (Hanwoo) bone (leg bone, foot and tail) infusion contains the various inorganic materials, collagen and chondroitin sulfate. All of this, a large quantity of collagen is included in Hanwoo infusion. Therefore, this study emphasized on the effects of collagen in the Hanwoo bone infusion. For the first time, Hanwoo bone infusions were directly added to the media of Human Dermal Fibroblast (NHDF-c) to test anti-aging effects. First, it was identified that growth rate of skin fibroblast was increased. Furthermore, the Hanwoo bone infusion increased a 50% of fibroblast collagen synthesis. Also, suppression of skin fibroblast aging was confirmed by treatment Hanwoo bone infusion. In conclusion, this study demonstrates the effects of infusion made from Hanwoo leg bone, foot and tail on anti-aging, wrinkle inhibiting and skin fibroblast elasticity maintaining. Therefore, this study identified that traditional infusion has effects that are good for skin elasticity.