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Illudins C_2 and C_3, New Illudin C Derivatives from Coprinus atramentarius ASI20013
LEE, IN-KY0UNG,JEONG, CHEOL-YOUN,CHO, SOO-MUK,YUN, BONG-SIK,KIM, YANG-SUP,YU, SEUNG-HUN,KOSHINO, HIROYUKI,YOO, ICK-DONG 충남대학교 생물공학연구소 1997 생물공학연구지 Vol.5 No.-
Ganoderma lucidum 균주는 인공 배지 상에서 광에 의하여 자실체 원기가 형성되었으며, 광질이 자실체 원기 형성에 미치는 영향을 조사한 결과, 자실체 원기 형성능을 가지고 있는 5균주중 3균주는 공시한 모든 형광등의 아래에서 즉, BLB, 순청색, 순녹색, 순황색, 순적색 형광등의 아래에서 자실체 원기가 형성되었고, 2균주는 BLB 형광등을 제외한 가시광선 영역에서 형성되었다. 그러나 암상태에서는 공시한 모든 균주가 자실체를 형성하지 않았다. 자실체 원기는 광도 0.05에서 10.0 μmol m^-2 s^-1의 범위에서 형성되었고, 광도가 0.5 μmol m^-2 s^-1 이상에서는 자실체 원기의 수가 감소하였다. 주기적인 광조사가 자실체 원기의 형성에 미치는 영향을 조사한 결과, G1-009 균주는 자실체 원기의 수와 무게가 연속광을 조사한 경우보다 증가하였다. 자실체 원기를 형성하기 위해서는 최소 4일간의 광조사가 필요하였다. 단색광의 조사가 자실체 원기의 형성에 미치는 영향을 조사한 결과 G1-003 균주는 400에서 800 nm의 모든 단색광 처리구에서 자실체 원기가 형성되었고, 그 외의 4균주는 400에서 500 nm, 그리고 700에서 750 nm 범위에서 형성되었다. The objective of this research was to determine the effect of light quality on formation of fruit body primordia (FBPs) of Ganoderma lucidum. To achieve this 5 isolates of the fungus that develops fruit body primordia on nutrient agar media were incubated with or without continuous irradiation. The fluorescent lamps used different colors such as black light blue (BLB), pure blue (P-B), pure green (P-G), pure yellow (P-Y) and pure red (P-R). Effect of periodic light and dark exposures on FBP formation of isolate G1-009 was investigated. The FBP formation in G. lucidum isolates was also tested under nomochromatic light produced by the combination of interference filters and colored glass filters. Three isolates produced FBPs under all kinds of fluorescent lamps, whereas two induced FBPs only under visible light except for BLB fluorescent lamp. However, these isolated did not form FBPs in the dark. The FBP was formed at light intensity from 0.05 to 10.0 μmol m^-2 s^-1, and begun to reduce its number as light intensity increase over 0.5 μmol m^-2 s^-1. When the isolate was incubated under periodic light and dark exposures, the number and weight of FBP increased as compared with those under continuous light. Initiation of FBP requires at least 4 days of light illumination. Although isolate G1-003 produced FBPs in a wide range of 400 to 800 nm, other four isolates had two effective regions 400 to 500 nm and 700 to 750 nm in FBP formation.
( Ky Young Cho ),( Byung Ho Choe ),( Hye Sook Park ),( Jeong Wan Seo ),( Jae Sung Ko ),( Jae Young Kim ),( Yong Joo Kim ),( Eell Ryoo ),( Sun Hwan Bae ),( Jea Geon Sim ),( Hye Ran Yang ),( Ji Ah Jung 대한소화기학회 2007 SIDDS Vol.9 No.-
Background/Aims: The identification of specific behaviors conducive to overeating or inactivity is the comerstone of obesity management. The aim of this study is to assess the detailed lifestyle associated with obesity in children and adolescents by questionnaires. Methods: A total of 352 children and adolescents aged 6 to 16 years were included from 7 hospitals between May 2006 and January 2007. They completed parent and self reported questionnaires developed by the Committee on Nutrition of the Korean Pediatric Society. We divided them into normal, overweight and obese group. Data were analyzed by SAS (P<0.05). Results: The type of feeding and weight at birth, past medical history, the kind of food and exercise did not differ significantly between the normal, overweight and obese group (P<0.05). In both children and adolescents, the proportion of sedentary activity (>9 hr/day: 10.17% vs 26.23% vs 27.54%) and inappropriate dietary behaviors (strong appetite, high eating speed, eating without hunger, late meal time, etc) were significantly higher in overweight and obese group than in normal group (P<0.05). In adolescents, overweight and obese group had a positive correlation with television viewing (>2 hr/day: 5.88% vs 20% vs 25.49%) and with computer time (>1 hr/day: 25.72% vs 56.67% vs 46.61%) (P<0.05). Conclusions: Obesity management should include modification of dietary behaviors and sedentary activity. Consultation using questionnaires will be efficient in pediatric office.
Association of gut microbiota with obesity in children and adolescents
Cho Ky Young 대한소아청소년과학회 2023 Clinical and Experimental Pediatrics (CEP) Vol.66 No.4
Pediatric obesity is among the most serious global health problems whose prevalence has increased over the past decade. Pediatric obesity increases concomitant health problems, including type 2 diabetes mellitus, hypertension, dyslipidemia, fatty liver disease, and psychological problems, which often progress into adulthood. The gut microbiota is a new factor in the development of obesity, which is affected by renowned risk factors such as diet, lifestyle, and socioeconomic status. This review aimed to describe the association between the gut microbiota and childhood obesity. According to advances in gene sequencing technologies, many findings of experimental animal and human studies of adults and children demonstrated that compositional and functional changes in the gut microbiota (dysbiosis) are associated with the development of obesity. Many studies have reported that an increased Firmicutes/Bacteroidetes (F/B) ratio is a biomarker of obesity susceptibility; however, with the rapid accumulation of data, meta-analyses of human gut microbiota and obesity showed no clear association between F/B ratio and obesity status. The contribution of the microbiota to obesity has been considered using multifactorial approaches, such as supplying additional calories to the host, modulating blood lipopolysaccharide levels, favoring fat storage, and affecting satiety. Probiotics are proposed to manipulate the gut microbiota population to improve obesity; however, their clinical application remains limited because trials have shown different results. Further studies are required to better understand the mechanisms underlying the observed association between the gut microbiota and pediatric obesity.
( Ky Young Cho ),( Tae Young Khil ),( Hye Mi Ahn ),( Sun Wha Lee ),( Jeong Wan Seo ) 대한소화기학회 2007 SIDDS Vol.9 No.-
Background/Aims: The intestinal pseudo-obstruction (IPO) is a rare and poorly understood manifestation of systemic lupus erythematosus (SLE), especially in children. It is characterized by clinical features of intestinal obstruction without identifiable obstructive lesion. Methods: We report a 13-year-old girl whose first symptom of SLE was IPO. The patient presented with a 3-day history of nausea, billous vomiting, abdominal distention and no stool passing. Results: Simple abdomen revealed mind dilatation with partial air-fluid level in the small intestine. Abdominal CT and methylcellulose small bowel studies showed massive ascites, engorgement of small mesenteric vessels, pleural effusion and diffuse bowel wall thickening of the gastric antrum, duodenum and jejunum. Delayed passage of contrast for 15 days after abdominal CT was suggestive of the decreased bowel motility. Laboratory finding were positive in ANA, anti-double stranded DNA, anti-Sm and lymphopenia. After a 10-day treatment with a high dose corticosteroid, the symptom was improved. Conclusions: IPO associated with SLE should be considered as a differential diagnosis for patients presenting the symptoms of intestinal obstruction. Early recognition of IPO in SLE and appropriate therapy are important for preventing complications and unnecessary surgery. Our case can raise awareness among pediatricians that although rare, IPO can be the presenting symptom of SLE in children.