A 14-DAY REPEATED DOSE TOXICITY OF POLYPORUS UMBELLATUS FRIES ADMINISTRAED BY ORAL GAVAGE IN F344 RATS

Hyoung-Yun Han,Ji-Young Kim,Sang-Joon Park,Byung-Sun Min,Jung-Im Huh 한국환경독성학회 2009 한국독성학회 심포지움 및 학술발표회 Vol.2009 No.11

Sublethal pulmonary toxicity screening of silica nanoparticles in rats after direct intratracheal instillation

Han Hyoung-Yun 한국독성학회 2022 Toxicological Research Vol.38 No.4

The present aimed to characterize the toxicity of silica nanoparticles in Sprague Dawley rats and determine the dose levels for a repeated-dose toxicity study. Silica nanoparticles ( SiO2, 20 nm and 50 nm) were administered as a single intratracheal instillation of standardized SiO2 20 nm (low dose, 200 μg/mL; high dose, 400 μg/mL) and 50 nm (low dose, 200 μg/mL; high dose, 400 μg/mL). Each group consisted of five male rats. We documented the mortality rate, clinical signs, body weight, bronchoalveolar lavage fluid analysis, hematological values, serum chemistry values, organ weight, gross findings at necropsy, and histopathological assessments. Rats treated with 200 μg/mL and 400 μg/mL SiO2 50 nm exhibited a decreased mean corpuscular volume, while those treated with 400 μg/mL of SiO2 50 nm showed increases in absolute monocyte and absolute lymphocyte count as well as prothrombin time. In addition, rats treated with 400 μg/mL SiO2 20 nm and 50 nm presented reduced creatinine, alanine aminotransferase, and sodium levels. Therefore, a single intratracheal instillation of SiO2 20 nm and 50 nm elicited no toxicity up to a dose of 400 μg/mL, and the approximate lethal dose of this test substance exceeded 400 μg/mL in male Sprague Dawley rats under the present experimental conditions.

Introductions of Japan laboratory animal technician certification

Hyoung-Yun Han 한국실험동물학회 2017 한국실험동물학회 학술발표대회 논문집 Vol.2017 No.8

Integrated transcriptomic analysis of liver and kidney after 28 days of thioacetamide treatment in rats

Han Hyoung-Yun,Park Se-Myo,Ko Je-Won,Oh Jung-Hwa,Kim Sang Kyum,Kim Tae-Won 한국독성학회 2023 Toxicological Research Vol.39 No.2

Thioacetamide (TAA) was developed as a pesticide; however, it was soon found to cause hepatic and renal toxicity. To evaluate target organ interactions during hepatotoxicity, we compared gene expression profiles in the liver and kidney after TAA treatment. Sprague–Dawley rats were treated daily with oral TAA and then sacrificed, and their tissues were evaluated for acute toxicity (30 and 100 mg/kg bw/day), 7-day (15 and 50 mg/kg bw/day), and 4-week repeated-dose toxicity (10 and 30 mg/kg). After the 4-week repeated toxicity study, total RNA was extracted from the liver and kidneys, and microarray analysis was performed. Differentially expressed genes were selected based on fold change and significance, and gene functions were analyzed using ingenuity pathway analysis. Microarray analysis showed that significantly regulated genes were involved in liver hyperplasia, renal tubule injury, and kidney failure in the TAA-treated group. Commonly regulated genes in the liver or kidney were associated with xenobiotic metabolism, lipid metabolism, and oxidative stress. We revealed changes in the molecular pathways of the target organs in response to TAA and provided information on candidate genes that can indicate TAA-induced toxicity. These results may help elucidate the underlying mechanisms of target organ interactions during TAA-induced hepatotoxicity.

A 14-Day Repeated Dose Toxicity of Polygalae Radix Administered by Oral Gavage in F344 Rats

Hyoung-Yun Han,SooNam Kim,Young-Su Yang,Mi-Jin Yang,Su-Cheol Han,Shin-Woo Cha,Byung-Sun Min 한국실험동물학회 2014 한국실험동물학회 학술발표대회 논문집 Vol.2014 No.8

Assessing the safety of an Ephedrae Herba aqueous extract in rats: A repeat dose toxicity study

Han, Hyoung-Yun,Huh, Jung-Im,Han, So-Ri,Kang, Myung-Gyun,Yoon, Seokjoo,Han, Ji-Seok,Lee, Byoung-Seok,Kim, Jeong Ah,Min, Byung-Sun Elsevier 2018 Regulatory toxicology and pharmacology Vol.94 No.-

<P><B>Abstract</B></P> <P>Ephedrae Herba (EH) has been used in Asian traditional herbal medicine to cure bronchial asthma, cold, flu, chills, fever, headache, nasal congestion, and cough. In this study, we evaluated the subchronic toxicity of an Ephedrae Herba aqueous extract (EHAE) in male and female F344 rats. The EHAE was administered orally daily at doses of 0, 125, 250, 500, and 1000 mg/kg bw/day for 13 weeks. Toxicological assessment was performed to determine mortality, clinical signs, and changes in body weight, food consumption, ophthalmological, urinary, hematological, and serum biochemical parameters, macroscopic and microscopic evaluations, and organ weights. We found that oral administration of EHAE to F344 rats for 13 weeks resulted in histopathological changes in the kidneys and salivary glands. In the kidneys, increased incidence and severity of tubular basophilia were observed in females administered 1000 mg/kg bw/day of the extract. In the salivary glands, acinar cell hypertrophy was observed in males administered 500 mg/kg bw/day and in both sexes administered 1000 mg/kg bw/day of the extract. All test article-treated groups of males and females administered ≥250 mg/kg bw/day showed increased absolute and relative salivary gland weights. Therefore, the NOAEL (No Observed Adverse Effect Level) was determined as 125 mg/kg bw/day for both sexes of rats under the present experimental conditions.</P> <P><B>Highlights</B></P> <P> <UL> <LI> There is little data in the literature on the toxic effects of Ephedrae Herba. </LI> <LI> Three males and two females in the highest dose group (1000 mg/kg bw/day) were found dead at weeks 1 and 9. </LI> <LI> In macroscopic observation, test article-related changes were observed in the kidneys and salivary glands. </LI> <LI> The no-observed-adverse-effect level was determined as 125 mg/kg bw bw/day for both sexes. </LI> </UL> </P>

Associated factors for the development of postpartum depression at 1 month postpartum

( Ji Hyoung Yook ),( Gye Hyeong Ahn ),( Yun Young Kim ),( You Jung Han ),( Si Won Lee ),( Min Hyoung Kim ),( Jin Hoon Chung ),( June Seek Choi ),( Hyun Kyung Ahn ),( Jung Yeol Han ),( Moon Young Kim ) 대한산부인과학회 2011 대한산부인과학회 학술대회 Vol.97 No.-

열차하중을 고려한 장대레일 궤도 해석

한상윤(Sang-Yun Han),강영종(Young-Jong Kang),한택희(Teak-Hee Han),임남형(Nam-Hyoung Lim),김정훈(Jung-Hun Kim) 한국철도학회 2011 한국철도학회 학술발표대회논문집 Vol.2011 No.10

At high rail temperature above the neutral temperature high compressive axial stresses will occur in the rails. High thermal axial force and vehicle loads cause the track to shift in a lateral direction and the formation of track geometry imperfections (track irregularity). When the thermal stress level and track irregularity with vehicle load reach a critical value the track loses stability. In many studies the stability of CWR tracks is analyzed. However these studies are only considered in temperature load. The main objective of this investigation was to estimate a new comprehensive realistic the stability of CWR tracks considering wheel load. The ballast resistance is changed by wheel load. When the wheel load is applied rails and ties are moved upward or downward. In this case the friction between ties and ballasts is decreased or increased. In this study the change of the ballast resistance of each tie was applied to the nonlinear analysis of CWR tracks.

임상 ; 한국 마더세이프 전문 상담 센터의 수유부 약물 상담 내용 분석; 5년간의 경험

육지형 ( Ji Hyoung Yook ),안현경 ( Hyun Kyung Ahn ),한정열 ( Jung Yeol Han ),한유정 ( You Jung Han ),김윤영 ( Yun Young Kim ),안계형 ( Gye Hyeong Ahn ),이시원 ( Si Won Lee ),김민형 ( Min Hyoung Kim ),정진훈 ( Jin Hoon Chung ),류 대한주산의학회 2011 Perinatology Vol.22 No.2

목적: 수유 중 약물 노출에 관한 정보는 상당히 부족한 실정이기에 한국 마더세이프 전문 상담 센터는 임산부뿐만 아니라 수유부에게도 약물에 대한 정보 제공 및 상담을 위해 전화 상담 센터를 열었다. 우리는 약물에 노출된 수유부 및 수유아에 대한 5년간의 상담 내용을 검토해 보았다. 방법: 2005년 1월부터 2010년 4월까지 전화 상담 센터를 통해 수유 중 약물 노출에 관해 상담한 수유부를 대상으로 하였고 전화를 통해 추적 관찰을 하였다. 질문 내용으로는 수유부 및 수유아의 인구학적 특성 외에도 약물 노출 후 발생한 증상 및 전반적인 수유에 관한 질문이었다. 결과: 모든 질의에 응답한 총 278 쌍의 수유모 및 수유아에 대해 수유 중 약물 노출에 대한 평가가 이루어졌으며, 이들중 대부분은 약물 노출에도 불구하고 수유 중 심각한 부작용은 없었다고 응답했다. 부작용은 단 3명(1.1%)의 수유아에게만 나타났으며, 20명(7.2%)의 수유부가 젖량 감소를 호소하였다. 상담 후 232명(83.5%)의 수유부는 수유를 지속하였지만, 20명(7.2%)은 일시적으로 수유 중단 후 재개하였고 26명(9.3%)은 영구적으로 수유를 중단하였다. 결론: 수유 중 약물은 수유부 및 영아에게 심각한 부작용을 일으키지는 않지만 많은 약물에 있어 안전성에 대한 정보가 확립되지 않았기에 임상의들은 치료적 이점과 수유아에 대한 위험성에 대해 갈등하게 된다. 이에 우리는 본 상담 내용의 분석을 통해 수유모 뿐만 아니라 임상의에게도 수유 중 약물에 대한 명확한 지식을 제공하고 올바른 수유 문화가 정착되기를 기대한다. Purpose: There is a dearth of information on maternal drug exposure during lactation. The Korean Mothersafe Professional Counseling Center launched helpline to provide information and clinical consultation service on drug safety during lactation as well as in pregnancy. Here, we reviewed our 5 years` experience of counseling with drug exposed breastfeeding mothers. Methods: The questionnaires were given to drug exposed breastfeeding mothers from January 2005 to April 2010 who contacted our helpline and follow-up survey data was collected by phone call. The questionnaires included lists of symptoms that exposed mothers experienced and that was observed in their infants, as well as demographic questions and questions about lactation. Results: A total of 278 mothers completed the survey and lactational exposure was estimated. Majority of them reported that their infants and themselves never experienced serious side effects of drugs during lactation. Only 3 (1.1%) babies reported side effects and 20 (7.2%) mothers reported decreased production of breast milk. Two hundred thirty two (83.5%) mothers continued breastfeeding after counseling. Lactation was stopped temporarily in 20 (7.2%) mothers and permanently in 26 (9.3%) mothers. Conclusion: Most of the drugs exposed during lactation did not cause serious side effects to infants and mothers. As many drugs have inadequate data to assure safety, the clinician is left with a dilemma as to where the balance of risks and benefits lie with respect to the mother and her baby. The author expect that analyses of these counseling will contribute to provide practical answers to clinicians as well as exposed mothers and to establish correct breastfeeding practice.

극소저체중출생아에서 출생 후 덱사메타손 투여시기에 따른 기관지폐이형성증의 양상

이해연 ( Hae Yun Lee ),이형진 ( Hyoung Jin Lee ),고지원 ( Ji Won Koh ),송인규 ( In Gu Song ),김세연 ( Sae Yun Kim ),정영화 ( Young Hwa Jung ),신승한 ( Seung Han Shin ),최창원 ( Chang Won Choi ),김이경 ( Ee Kyung Kim ),김한석 ( Han 대한주산의학회 2015 Perinatology Vol.26 No.4

목적: 기관지폐이형성증의 예방과 치료를 위해서 사용하는 부신피질 스테로이드의 효과에 대해서는 잘 알려져 있지만 부작용에 대한 우려로 부신피질 스테로이드 투여가 지연되어 충분한 효과를 얻지 못하는 경우가 많다. 본 연구에서는 극소저체중출생아에서 기관지폐이형성증 예방 및 치료목적으로 부신피질 스테로이드를 투여한 경우, 투여시기에 따른 임상적 예후를 비교하여 적절한 투여시기를 알아보고자 하였다. 방법: 2008년 1월부터 2014년 9월까지 서울대학교병원과 분당서울대병원에 입원한 극소저체중출생아 중 부신피질 스테로이드를 투여 받은 미숙아 56명을 후향적으로 분석하였다. 대상 환아들을 출생 후 4주 이전과 4주 이후에 부신피질 스테로이드를 투여한 경우로 나누어서 두 군간에 호흡기계 예후에 대해서 조사하였다. 결과: 부신피질 스테로이드를 생후 4주 이전에 투여한 조기 투여군은 30명, 생후 4주 이후에 투여한 후기 투여군은 26명이였다. 두 군간 재태 주수, 출생체중 등 임상적인 특성에는 차이가 없었다. 조기 투여군에서는 후기 투여군과 비교하여 스테로이드 투여 직전의 호흡중증도 지수와 산소요구량이 높았으나, 출생에서부터 발관이 이루어지는 기간은 짧았다(32.0일 vs 63.5일, P=0.001). 또한 후기 투여 군에서는 중증의 기관지폐이형성증이 발생 빈도가 높았으며, 교정연령 12개월에 뇌성마비가 진단된 경우는 두 군에서 차이가 없었다. 여러 관련된 인자들을 보정하였을 때 중증의 기관지폐이형성증은 출생 4주 이후에 부신피질 스테로이드를 투여하는 것과 의미 있는 연관성을 보였다(adjusted OR 17.14 [1.29-227.52], P=0.031). 결론: 고위험군 환아에서 신경발달학적 부작용 없이 기계환기를 최소화 하고, 중증의 기관지폐이형성증 발생을 막기 위한 부신피질 스테로이드 사용은 그 안전성과 이득을 고려하여 생후 1주에서 4주 사이에 신중하게 투여 하는 것이 필요하다. Purpose: Corticosteroids has been used for treatment and prophylaxis of bronchopulmonary dysplasia (BPD) in preterm infants. However, administration of corticosteroids could be delayed due to its potential harmful effects on neurodevelopment. The aim of this study was to evaluate the adequate dexamethasone administration timing in very low birth weight infants. Methods: Medical records of 56 VLBW infants who were admitted to neonatal intensive care unit of Seoul National University Children’s Hospital and Seoul National University Bundang Hospital between January 2008 and September 2014 were collected retrospectively. Study population were divided into early administration group (dexamethasone administration before 4 weeks of postnatal days) and late administrationgroup (after 4 weeks) and respiratory morbidities were compared between groups. Results: There were no significant differences in clinical characteristics between early administration group (n=30) and late administration group (n=26). Respiratory severity score and oxygen needs at 7 days after birth and before administering dexamethasone were comparable. Extubation was done earlier postnatal days in early administration group. Incidence of severe BPD was higher in the late administration group. There was no significant difference in diagnosed with cerebral palsy (CP) at 12 months of corrected age. When adjusting for multiple risk factors, administration of dexamethasone 4 weeks after birth and severe of BPD showed a significant association (adjusted OR 17.14 [1.29-227.52], P=0.031). Conclusion: Administration of dexamethasone in order to minimize ventilator care and to reduce severe BPD might be done between 1 week and 4 weeks after birth in very low birth weight infants.