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이원규,Hye-Jung Kim,Hye-Ki Min,강운범,이철주,이상원,김익영,이승택,권오승,Yeon Gyu Yu5* 대한화학회 2007 Bulletin of the Korean Chemical Society Vol.28 No.9
Proteomic analyses of synaptic vesicle fraction from rat brain have been performed for the better understanding of vesicle regulation and signal transmission. Two different approaches were applied to identify proteins in synaptic vesicle fraction. First, the isolated synaptic vesicle proteins were treated with trypsin, and the resulting peptides were analyzed using a high-pressure capillary reversed phase liquid chromatography/tandem mass spectrometry (cRPLC/MS/MS). Alternatively, proteins were separated by two-dimensional gel electrophoresis (2DE) and identified by matrix-assisted laser desorption ionization mass spectrometry (MALDI-TOF/MS). Total 18 and 52 proteins were identified from cRPLC/MS-MS and 2DE-MALDI-TOF-MS analysis, respectively. Among them only 2 proteins were identified by both methods. Of the proteins identified, 70% were soluble proteins and 30% were membrane proteins. They were categorized by their functions in vesicle trafficking and biogenesis, energy metabolism, signal transduction, transport and unknown functions. Among them, 27 proteins were not previously reported as synaptic proteins. The cellular functions of unknown proteins were estimated from the analysis of domain structure, expression profile and predicted interaction partners.
정신분열병과 Neurotensin 수용체 유전자 다형성의 연합 연구
이유상,김형배,한진희,채영규,이정식,이혜순,주연호,김형섭,최인근,양병환 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.6
연구목적: Neurotensin(NT)은 NT수용체와 결합하여 그 효과를 나타내는 neuromodulator 혹은 neurotransmitter로서 대뇌에서 도파민의 분비를 조절하는데 중요한 역할을 한다. 근래의 연구에 의하면 NT와 그 수용체는 대뇌에서 항정신병 약물의 효과를 매개하는 것으로 생각되고 있으며 약물치료를 받지 않은 정신분열병 환자의 뇌척수액에서 NT의 양이 적으로 보고되고 있어 이들은 정신분열병과 깊은 관련을 가지고 있을 것으로 추정된다. 최근 NT수용체의 유전자의 3`인접영역에서 정보가치가 높은 4 염기반복 다형성이 발견되어 이를 유전 표지자로 하여 정신분열병과의 연합을 알아보았다. 방 법: 서로 혈연관계에 있지 않은 정신분열병 환자 120명(남자 91명, 여자 29명)과 정상 대조군 106명(남자 84명, 여자 22명)을 대상으로 하였다. PANSS를 사용하여 양성 및 음성을 알아보았다. 말초혈액에서 DNA를 분리한 후에 중합효소연쇄반응을 사용하여 3`인접영역에 있는 4 염기 반복 다형성을 증폭하였고 silver staining한 후에 유전자형을 관찰하였다. chi-square 검증과 Bonferroni`s correction을 사용하여 환자군과 정상 대조군간의 대립유전자 빈도의 차이를 알아보았다. 또한 양성 및 음성 환자군으로 나누어 차이를 알아보았다. 결 과: 총 23개의 대립유전자가 관찰되었으며, 399bp의 대립유전자(A10)의 빈도가 환자군보다 정상대조군에서 통계적으로 유의하게 높았다(χ²=16.49, df=1, p<0.001). 음성 정신분열병 환자군과 정상대조군 사이의 비교에서는 정상대조군의 A10의 빈도가 환자군보다 유의하게 높았다(χ²=21.33, df=1, p<0.001). 성별 비교에서 남자 정신분열병 환자군은 대조군에 비하여 A10의 분포가 유의하게 적었다. (χ²=13.71, df=1, p<0.001) 결 론: NT 수용체 유전자와 정신분열병사이에 음성연합이 관찰되었다. NT 수용체 유전자가 일부 정신분열병의 발병과정에서 확실하지는 않지만 어떤 종류의 보호기능을 할 수도 있다는 것을 암시한다. Objectives: Neurotensin(NT), of which functions are evoked by its interaction with neurotensin receptors(NTR), coexists with mesolimbic dopamine and regulates endogenous dopamine release. Recent studies have shown that NT with NTR exerts neuroleptic-like activity within the central nervous system and may play an important role in the pathogenesis and in the treatment of schizophrenia. We have examined the gentic association between schizophrenia and tetranucleotide repeat polymorphism in the 3-flanking region of the NTR gene to investigate the possible contribution of the NTR gene to the schizophrenia susceptibility. Methods: Among 23 alleles identified, the subjects were 120 patients(male 91, female 29)with schizophrenia and 106 normal healthy controls(male 84, female 22). They were unrelated native Korean. PANSS was used to determine positive or negative subgroup in the schizophrenic patients. Using polymerase chain reaction and polyacrylamide gel electrophoresis, tetranucleotide repeat polymorphism(CCTT and CTT) in the 3`-flanking region of NTR gene was observed. For a comparison of NTR gene`s allelic frequencies between patients with schizophrenia and normal healthy controls, chi-square test and Bonferroni`s correction was performed. Results: The frequency of A10 allele(base pair size=399)was significantly higher in normal healthy controls than schizophrenia(χ²=16.4902, df=1, p<.000). In the comparison between schizophrenic patients with negative symptoms and normal controls, the frequency of A10 allele was significantly higher in normal healthy control subjects than patients with schizophrenia(χ²=21.33, df=1, p<0.001). In the case of male, the frequency of A10 allele of schizophrenia was significantly higher than normal controls(χ²=13.71, df=1, p<0.001). Conclusions: NTR gene was negatively associated with schizophrenia. NTR gene`s tetranucleotide repeat polymorphism may provide some protective function against schizophrenia.
연산 증폭기 (TL494) 를 이용한 DC/DC 승압 컨버터 제어 시스템 설계
정구종,이혜연,문경주,정유석,이준영,손영익 明知大學校 産業技術硏究所 2009 産業技術硏究所論文集 Vol.28 No.-
A simple control system using an op Amp (TL494) is presented for a DC/DC boost converter. The DC/DC boost converters have many industrial applications including renewable energy sources and hybrid automobiles. The problem of regulating the output voltage in the presence of load variation has been a research subject of interest for many years. After computer simulations with SimPower system of Matlab Simulink, we have developed the converter system in university laboratory according to the design specifications. Since the chip TL494 has both op Amp and PWM comparator even beginners can easily develop the control system. Experimental results show that the proposed system has some robustness with respect to the load variation.
Yu, Yeon-Sil,Bae, Se-Yeon,Kim, Hye-Min,Kim, Ye-Jin,Chu, Nag-Bum,Chu, Nag-Kyun,Kang, Jae-Seung,Lee, Wang-Jae The Korean Association of Immunobiologists 2011 Immune Network Vol.11 No.4
It is already known that high concentration of vitamin C induces apoptosis on tumor cells. However, there is no report regarding the function of vitamin C on the modulation of immune susceptibility of cancer. Therefore, we investigated whether vitamin C can modulate immune susceptibility of tumor cells, especially on the induction of Fas-mediated apoptosis. First, the optimal concentration of vitamin C, which cannot induce damages on tumor cells for 36 hrs. We found that 2 mM of vitamin C did not show harmful effect. In addition, the optimal concentration of agonistic anti-Fas Abs for 18 hrs was examined. As a result, 400 ng/ml of agonistic anti-Fas Abs did not induce apoptosis on tumor cells. Next, we tried to find the effect of 2 mM of vitamin C on the modulation of the susceptibility to agonistic anti-Fas Abs. When tumor cells were cultured with 400 ng/ml of agonistic anti-Fas Abs for 18 hrs, after pre-treatment with 2 mM of vitamin C for 24 hrs, viability of cells was decreased. Interestingly, we found that the expression of Fas (CD95) and MHC class I was increased by the treatment of vitamin C. Taken together, vitamin C increases the susceptibility of tumor cells to anti-Fas Abs and the expression of Fas (CD95) and MHC class I on tumor cells.
Extracting and Clustering of Story Events from a Story Corpus
( Hye-yeon Yu ),( Yun-gyung Cheong ),( Byung-chull Bae ) 한국인터넷정보학회 2021 KSII Transactions on Internet and Information Syst Vol.15 No.10
This article describes how events that make up text stories can be represented and extracted. We also address the results from our simple experiment on extracting and clustering events in terms of emotions, under the assumption that different emotional events can be associated with the classified clusters. Each emotion cluster is based on Plutchik’s eight basic emotion model, and the attributes of the NLTK-VADER are used for the classification criterion. While comparisons of the results with human raters show less accuracy for certain emotion types, emotion types such as joy and sadness show relatively high accuracy. The evaluation results with NRC Word Emotion Association Lexicon (aka EmoLex) show high accuracy values (more than 90% accuracy in anger, disgust, fear, and surprise), though precision and recall values are relatively low.
Temporal Search Algorithm for Multiple-Pedestrian Tracking
( Hye-yeon Yu ),( Young-nam Kim ),( Moon-hyun Kim ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.5
In this paper, we provide a trajectory-generation algorithm that can identify pedestrians in real time. Typically, the contours for the extraction of pedestrians from the foreground of images are not clear due to factors including brightness and shade; furthermore, pedestrians move in different directions and interact with each other. These issues mean that the identification of pedestrians and the generation of trajectories are somewhat difficult. We propose a new method for trajectory generation regarding multiple pedestrians. The first stage of the method distinguishes between those pedestrian-blob situations that need to be merged and those that require splitting, followed by the use of trained decision trees to separate the pedestrians. The second stage generates the trajectories of each pedestrian by using the point-correspondence method; however, we introduce a new point-correspondence algorithm for which the A* search method has been modified. By using fuzzy membership functions, a heuristic evaluation of the correspondence between the blobs was also conducted. The proposed method was implemented and tested with the PETS 2009 dataset to show an effective multiple-pedestrian-tracking capability in a pedestrian-interaction environment.
cDNA Library Construction of Aphis gossypii Using Gateway Cloning System
Hye-Ri Kwon,Jung-Kyu Kim,Na-Yeon Ko,Yu-Bin Jung,Chan-yeong Kang,Tae-Hee Ryu,Mi-Ja Seo,Hyoun-Sub Lim,Yong-Man Yu,Young-Nam Youn 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.10
Aphis gossypii was widely distributed throughout the tropical, subtropical and temperate zone. The chemical control of A. gossypii is becoming problem because it was rapidly appeared resistance expression to chemicals. We will attempt to resolve the this problem using RNAi technique. Besides, RNAi technology can be helpful to study the target genes of A. gossypii. In this study we produce cDNA library construction using gateway cloning system for selecting target gene in order to control of A. gossypii using RNAi. As a result, the 100-400bp of insert size, which is appropriate for RNAi was confirmed. Most of insert gene is associated with A. gossypii, after that insert sequence was compared with DNA databases and EST databases using NCBI blast search. Consequentially, A. gossypii of cDNA library with the titer of 3.15x105 clones were completed. And we will perform the LR recombination to transfer cDNA library into TRV2 (tobacco rattle virus) vector with att site. Then, after performing transformation using Agrobacterium tumefaciens (GV 2260), we inoculated to cucumber with A. tumefaciens. An insecticidal effect or a repellent activity against A. gossypii by changing behavior in transgenic cucumber plants were conformed. Also, the selecting target gene in order to control A. gossypii using RNAi may be provided.
나혜연 ( Hye Yeon Na ),송영화 ( Young Hwa Song ),김병주 ( Byoung Ju Kim ),유진호 ( Jin Ho Yu ),홍수종 ( Soo Jong Hong ),이소연 ( So Yeon Lee ) 대한소아알레르기호흡기학회(구 대한소아알레르기 및 호흡기학회) 2009 소아알레르기 및 호흡기학회지 Vol.19 No.2
목적: 아토피피부염이 지속될 가능성이 높은 2세 이하의 중증 아토피피부염 환자에서 비아토피성 아토피피부염이 차지하는 정도와 IgE, 호산구, 식품, 흡입 항원에 대한 감작과 아토피피부염의 중증도와의 연관성을 보고자 하였다. 방법: 한강성심병원과 서울아산병원을 방문한 2세 미만의 중증 아토피피부염 환자 215명 (남아123명, 여아 92명)을 대상으로 SCORAD를 측정하고 혈액검사로 총 호산구 수, 호산구 분율, 혈청 eosinophil cationic protein (ECP), 혈청 총 IgE, 항원 특이 IgE 12종(난백, 우유, 콩, 땅콩, 밀가루, 생선, D. pteronyssinus (D.p), D. farinae (D.f), 개, 고양이, 바퀴벌레, alternaria)을 검사하였다. 아토피성 아토피피부염은 혈청 총 IgE가 200 IU/mL 이상이거나 항원 특이 IgE에 양성인 경우로 정의하였다. 결과: 전체 환자의 평균 SCORAD 값은 69.0이었다. 아토피성 아토피피부염은 161명(75%)으로 비아토피성 아토피피부염 환자보다 많았으며 이 두 환자군간의 SCORAD 점수, 총 호산구 수, 혈청총 IgE, 호산구 분율, 혈청 ECP는 아토피성 아토피피부염에서 높았다. 아토피성 아토피피부염 환자군에서 식품에 대한 혈청 특이 IgE 감작은 난백이 76.4%로 가장 많았고 우유, 밀가루, D.f, D.p의 감작률은 각각 58.4%, 40.4%, 49.1%, 46.6%이었다. 감작된 식품 항원의 개수가 많을수록 SCORAD 점수도 높은 것으로 나타났으나(r=0.241, P=0.037), 감작된 흡입 항원의 개수와는 연관성을 보이지 않았다. 결론: 2세 미만의 중증 아토피피부염 환자에서 아토피성 아토피피부염 환자가 비아토피성 아토피피부염 환자보다 차지하는 비율과 중증도가 높고, 흡입 항원에 대한 감작보다는 식품 항원에 대한 감작 개수와 정도가 중증도에 영향을 준다. 따라서 2세 이하의 아토피피부염에서 식품 항원에 대한 감작 정도를 파악하는 것은 환자의 중증도를 예측하는데 도움이 될 수 있을 것이며 흡입 항원에 대한감작이 있는 경우는 향후 추적 관찰을 통해 호흡기알레르기 질환 발생 여부를 보는 것이 필요할 것으로 생각된다. Purpose: Atopic dermatitis (AD) is the allergic disease which appears during infancy. This study aimed to find associations between clinical parameters, allergen sensitization and severity of AD in chidren under 2 years. Methods: Two hundred fifteen patients (123 boys, 92 girls) under 2 years with severe AD who visited the department of pediatrics of Hangang Sacred Heart Hospital and Asan Medical Center were enrolled. We checked the severity scoring of atopic dermatitis (SCORAD), total eosinophil count, eosinophil ratio, eosionphil cationic protein (ECP), total IgE and specific IgE to 12 allergens [egg white, milk, soybean, peanut, wheat, fish, Dermatophagoides pteronyssinus (D.p), Dermatophagoides farinae (D.f), dog, cat, cockroach, alternaria]. Atopic AD was defined as serum total IgE 200 IU/mL or specific IgE positive. Results: The mean of SCORAD was 69.0. Atopic AD was 161 (75%). There were no differences in age between atopic and non-atopic AD. However, SCORAD, IgE, eosinophil, and ECP were the higher in atopic AD. Sensitization to food allergen was the highest in egg white (76.4%), followed by milk (58.4%) and aeroallergens such as D.f (49.1%) and D.p (46.6%). The more the number of sensitized food allergens, the higher the SCORAD appeared. However, it did not show any relationships with the number of sensitized aeroallergens. Conclusion: The percentage and severity were higher in atopic AD than non-atopic AD. The number of sensitized to food allergen affect the severity. Therefore, understanding the sensitization to food allergens may be more helpful in predicting severity than sensitization to aeroallergen. In cases of positive reaction to aeroallergens, it is necessary to observe the development of respiratory allergic disease. [Pediatr Allergy Respir Dis(Korea) 2009;19:146-154]