Synthesis of the Sum and Difference Patterns by a Hybrid Real/Integer-Coded Invasive Weed Optimization

Hua Guo,Chenjiang Guo,Jun Ding 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.4

The optimization of the sum and difference patterns for monopulse antennas by a hybrid real/integer-coded invasive weed optimization (IWO) is introduced in this paper. The whole array aperture is divided into several subarrays. The configuration and weight of each subarray are optimized. In order to reduce the difficulty of designing the feeding networks of the array antenna, the elements of the same subarray stay together. Since only the weight and elements number of each subarray is optimized, the number of the optimized parameters is reduced significantly which will reduce the complexity of the simulation procedure. Several numerical simulations are applied to validate the effectiveness of the proposed approach.

Poster Session 2 : Expression profiling of cellular process in uterine cervical cancer omic approaches and its correlation with array-CGH

( Hua Guo Ding ),( Su Mi Bae ),( Sun Young Kwak ),( Hyun Jin Min ),( Ji Yeun Noh ),( Byoung Don Han ),( Woong Shick Ahn ) 한국생화학분자생물학회 (구 한국생화학회) 2005 생화학분자생물학회 춘계학술발표논문집 Vol.2005 No.-

Poster Session 2 : Oral Presentation ; Molecular Cancer Biology : Expression profiling of cellular process in uterine cervical cancer omic approaches and its correlation with array-CGH

( Hua Guo Ding ),( Su Mi Bae ),( Sun Young Kwak ),( Hyun Jin Min ),( Ji Yeun Noh ),( Byoung Don Han ),( Woong Shick Ahn ) 한국생화학분자생물학회 (구 한국생화학회) 2005 62회 KSBMB Annual Meeting in 2005 Vol.- No.-

Three Dimensional Reconstruction of Bone-Cartilage Transitional Structures Based on Semi-Automatic Registration and Automatic Segmentation of Serial Sections

Hua Guo,Zheng-Wei Xu,Bao-Rong He,Ding-Jun Hao,Wei-Guo Bian 한국조직공학과 재생의학회 2014 조직공학과 재생의학 Vol.11 No.5

A well-defined three-dimensional (3-D) reconstruction of bone-cartilage transitional structures is crucial for the osteochondral restoration. This paper presents an accurate, computationally efficient and semi-automated algorithm for the alignment and segmentation of two-dimensional (2-D) serial to construct the 3-D model of bonecartilage transitional structures. Entire system includes the following five components: (1) image harvest, (2) image registration, (3) image segmentation, (4) 3-D reconstruction and visualization, and (5) evaluation. A computer program was developed in the environment of Matlab for the semi-automatic alignment and automatic segmentation of serial sections. Semi-automatic alignment algorithm based on the position’s cross-correlation of the anatomical characteristic feature points of two sequential sections. A method combining an automatic segmentation and an image threshold processing was applied to capture the regions and structures of interest. SEM micrograph and 3-D model reconstructed directly in digital microscope were used to evaluate the reliability and accuracy of this strategy. The morphology of 3-D model constructed by serial sections is consistent with the results of SEM micrograph and 3-D model of digital microscope.

BAC Chip을 이용한 자궁경부편평상피세포암 유전자의 증폭과 감소

정국화 ( Guo Hua Ding ),배수미 ( Su Mi Bae ),곽선영 ( Sun Young Kwak ),민현진 ( Hyun Jin Min ),이애리 ( Ae Ry Lee ),유희정 ( Hee Jeong Yu ),남궁정 ( Jeong Namkoong ),오은경 ( Eun Kyeong Oh ),신재은 ( Jae Eun Shin ),최지향 ( Ji Hya 대한산부인과학회 2006 Obstetrics & Gynecology Science Vol.49 No.9

목적: 자궁경부 편평상피세포암에서 array CGH법을 이용하여 유전자 발현의 양상을 조사함으로써 자궁경부 정상조직과 암 조직에서 유전자 발현의 차이에 대하여 알아보고자 하였다. 연구 방법: 자궁경부 편평상피세포암 조직 15예를 사용하였다. 조직을 파라핀에 고정하고, 10㎛씩 절단하여 헤마톡실린과 에오신염색을 한 다음, 미세절제술을 이용하여 DNA를 추출하였다. 1,440개의 human BACs으로 구성된 BAC array에 Cy5-dCTP와 Cy3-dCTP로 각각 표지된 정상과 자궁경부 편평상피세포암의 DNA를 교잡시켰으며, 정상과 환자군의 변이가 0.25배 이상 변화된 BAC clone에 포함된 유전자를 선별하였다. array CGH법에서 발현의 변화를 보인 유전자는 중합연쇄효소반응을 이용하여 그 유전자의 발현정도를 확인하였다. 결과: 자궁경부 편평상피세포암 70% 이상에서 SKI, FLJ13941, ZC3H3, GSDMDC1, PP3856, EEF1D,SOLH, LOC146325, FLJ36208, PIGQ, RAB40C, CYHR1, KIFC2, FOXH1, PPP1R16A, GPT, LOC113655, RECQL4, LRRC14, LRRC24, MGC70857, KIAA1688, PRKCZ, C1orf86, LOC440554, HDAC9, WDR8, TP73, KIAA0495, ESRRA, HSPC152, PRDX5, FLJ37970, RPS6KA4, TSC2, PKD1, RAB26, TRAF7 등 유전자를 포함하는 BAC clone들의 증폭과 PTPRG, LOC389432, SPATA19, LOC283172, KIAA0087, LOC442614, LOC386610 등 유전자를 포함하는 BAC clone들의 감소를 발견하였으며, 중합연쇄효소반응을 통하여 GSDMDC1, ABCF3, PCOLN3, SOLH, PRKCZ 등 유전자의 증폭과 NR3C2, CSF2, MSH6, MTR, PDE1C, ELF5, TXNDC10, SOD1 등 유전자의 감소를 발견하였다. 결론: 자궁경부암 조직에서 Array CGH를 이용하여 유전자발현의 양상을 조사한 결과, BAC clone내에 존재하는 유전자의 발현변화에 관한 정보를 얻을 수 있었다. 이는 자궁경부암의 암화과정에 대해 이해하는데 도움이 되리라 생각한다. Objective: Cervical cancer has long been linked to the sexually transmitted human papillomavirus (HPV), and the oncoproteins E6 and E7 disrupt the functions of tumour suppressor genes, resulting in genetic alteration. It was shown that loss of heterozygosity at 6p is a common genetic alteration in cervical cancer. However, the molecular genetics of cancer have only recently been understood, and for the development of cervical cancer additional genetic alterations in host cell genes are required. The present study has identified the differential changes of the cervical cancer-associated genetic alterations by a genome-wide array based comparative genomic hybridization (array-CGH). Methods: We analyzed 15 cases of cervical cancer from St. Mary`s hospital of The paraffin-fixed tissue samples were microdissected under microscope and DNA was extracted by the procedures of proteinase K digestion and chloroform extraction. Array-based CGH and genomic PCR were carried out with statistical analyses such as hierarchical clustering and Gene Ontology. The BAC array used in this study consisted of 1,440 human BACs, the space among the clones were approximately 2.08 megabase (Macrogen, Seoul, Korea). Results: All of 15 cases of cervical cancer showed specific gains and losses. The analysis limit of average gains and losses was 53%. A significant positive correlation was found between 1p36.32, 3p14.2, 3q27.1, 7p21.1, 8q24.3 and 11q13.1 changes through the cervical carcinogenesis. The high-level of gain regions, BAC clones encoded GSDMDC1, RECQL4, TP73, ABCF3, ALG3, HDAC9, ESRRA and RPS6KA4 genes. Frequently gained BAC clones encoded genes were PRSS8, FUS, COL18A1, PCOLN3, MAFG and ASPSCR1. The genes encoded by frequently lost BAC clones were PTPRG, GRM7, ZDHHC3, EXOSC7, LRP1B and NR3C2. Also, hierarchical clustering of the expression data readily distinguished genomic alterations in cervical cancer. A subset of cellular processes from each gene was clustered by Gene Ontology database. Conclusion: Using Array-CGH, genomic alterations related to cervical cancer were identified to determine whether induction of chromosomal imbalances occurs prior to carcinogenesis. The high resolution of array-CGH combined with human genome database would give a chance to find out possible target genes present in the gained or lost clones.

Full-scale testing and modeling of the mechanical behavior of shield TBM tunnel joints

Ding, Wen-Qi,Peng, Yi-Cheng,Yan, Zhi-Guo,Shen, Bi-Wei,Zhu, He-Hua,Wei, Xin-Xin Techno-Press 2013 Structural Engineering and Mechanics, An Int'l Jou Vol.45 No.3

For shield TBM (Tunnel Boring Machine) tunnel lining, the segment joint is the most critical component for determining the mechanical response of the complete lining ring. To investigate the mechanical behavior of the segment joint in a water conveyance tunnel, which is different from the vehicle tunnel because of the external loads and the high internal water pressure during the tunnel's service life, full-scale joint tests were conducted. The main advantage of the joint tests over previous ones was the definiteness of the loads applied to the joints using a unique testing facility and the acquisition of the mechanical behavior of actual joints. Furthermore, based on the test results and the theoretical analysis, a mechanical model of segment joints has been proposed, which consists of all important influencing factors, including the elastic-plastic behavior of concrete, the pre-tightening force of the bolts and the deformations of all joint components, i.e., concrete blocks, bolts and cast iron panels. Finally, the proposed mechanical model of segment joints has been verified by the aforementioned full-scale joint tests.

Preparation and Biocompatibility Study of Contrast-Enhanced Hernia Mesh Material

Ding Xuzhong,Zhu Jiachen,Liu Anning,Guo Qiyang,Cao Qing,Xu Yu,Hua Ye,Yang Yumin,Li Peng 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.4

BACKGROUND: Meshes play a crucial role in hernia repair. However, the displacement of mesh inevitably leads to various associated complications. This process is difficult to be traced by conventional imaging means. The purpose of this study is to create a contrast-enhanced material with high-density property that can be detected by computed tomography (CT). METHODS: The contrast-enhanced monofilament was manufactured from barium sulfate nanoparticles and medical polypropylene (PP/Ba). To characterize the composite, stress tensile tests and scanning electron microscopy (SEM) was performed. Toxicity and biocompatibility of PP/Ba materials was verified by in vitro cellular assays. Meanwhile, the inflammatory response was tested by protein adsorption assay. In addition, an animal model was established to demonstrate the long-term radiographic effect of the composite material in vivo. Subsequent pathological tests confirmed its in vivo compatibility. RESULTS: The SEM revealed that the main component of the monofilament is carbon. In vitro cell experiments demonstrated that novel material does not affect cell activity and proliferation. Protein adsorption assays indicated that the contrast-enhanced material does not cause additional inflammatory responses. In addition, in vivo experiments illustrated that PP/Ba mesh can be detected by CT and has good in vivo compatibility. CONCLUSION: These results highlight the excellent biocompatibility of the contrast-enhanced material, which is suitable for human abdominal wall tissue engineering.

Silicon Mediated Different Roles in Alleviation of Cadmium Toxicity in Two Cypress Varieties

Bin Guo,Wenhao An,Nengfei Ding,Chen Liu,Qinglin Fu,Hua Li,Ningyu Li,Yicheng Lin 한국토양비료학회 2014 한국토양비료학회 학술발표회 초록집 Vol.2014 No.6

Influence of Lateral Pressure on the Mechanical Performance of Bolted Specimens under Biaxial Compression

Shuxue Ding,Peilin Jiang,Jing Qiao,Hua Nan,Lei Xu,Jiaqi Guo,Sijiang Wei 대한토목학회 2024 KSCE Journal of Civil Engineering Vol.28 No.1

The influence of lateral pressure (2) on the stability of the surrounding rock of roadway increases after excavation. The rock bolt is usually used to improve the stability of the surrounding rock. Therefore, it is important to understand the biaxial compressive behaviors of the bolted samples influenced by lateral pressure. In this study, biaxial compression experiments are carried out to investigate the biaxial compressive properties of bolted rock samples influenced by lateral pressure. The results show that bolting can improve the peak strength, inhibit the post-peak strength attenuation and reduce the pre-peak brittleness of the bolted rock mass. With the increase of lateral pressure, the strength and elastic modulus of the bolted samples increases approximately parabolic and linearly, respectively. The failure modes of the bolted samples influenced by lateral pressure can be divided into splitting failure and compression-shear failure. A reinforcement mechanism of bolt is observed, namely axial action, axial and transverse action at the pre- and post-peak stages of the bolted samples. Based on a modified true triaxial Mohr-Coulomb strength criterion and this bolt reinforcement mechanism, a new method for calculating the equivalent cohesion and equivalent internal friction angle of the bolted specimens under biaxial compression is proposed. The research results of this paper have certain reference value for the stability control of roadway

Protein Expression Profile using Two-Dimensional Gel Analysis in Squamous Cervical Cancer Patients

배수미,민현진,Guo Hua Ding,곽선영,조영래,남계현,박충학,김용완,김종국,한병돈,이영주,김도강,안웅식 대한암학회 2006 Cancer Research and Treatment Vol.38 No.2

Purpose: Screening in cervical cancer is now progressingto discover candidate genes and proteins that mayserve as biological markers and that play a role in tumorprogression. We examined the protein expression patternsof the squamous cell carcinoma (SCC) tissues fromKorean women with using two- dimensional polyacrylamidegel electrophoresis (2-DE) and matrix assistedlaser desorption/ionization-time of flight (MALDI- TOF)mass spectrometer.Materials and Methods: Normal cervix and SCC tissueswere solubilized and 2-DE was performed using pH 3~10linear IPG strips of 17 cm length. The protein expressionwas evaluated using PDQuest 2-D softwareTM. The differentiallyexpressed protein spots were identified with aMALDI-TOF mass spectrometer, and the peptide massspectra identifications were performed using the Mascotprogram and by searching the Swiss-prot or NCBInrdatabases.Results: A total of 35 proteins were detected in SCC.17 proteins were up-regulated and 18 proteins weredownregulated.Among the proteins that were identified, 12proteins (pigment epithelium derived factor, annexin A2and A5, keratin 19 and 20, heat shock protein 27, smoothmuscle protein 22 alpha, α-enolase, squamous cell carcinomaantigen 1 and 2, glutathione S-transferase andapolipoprotein a1) were protein previously known to beinvolved in tumor, and 21 proteins were newly identifiedin this study.Conclusion: 2-DE offers the total protein expressionprofiles of SCC tissues; further characterization of thesedifferentially expressed proteins will give a chance toidentify the badly needed tumor-specific diagnostic markersfor SCC. (Cancer Res Treat. 2006;38:99-107) Purpose: Screening in cervical cancer is now progressing to discover candidate genes and proteins that may serve as biological markers and that play a role in tumor progression. We examined the protein expression patterns of the squamous cell carcinoma (SCC) tissues from Korean women with using two- dimensional polyacrylamide gel electrophoresis (2-DE) and matrix assisted laser desorption/ionization-time of flight (MALDI- TOF) mass spectrometer.Materials and Methods: Normal cervix and SCC tissues were solubilized and 2-DE was performed using pH 3~10 linear IPG strips of 17 cm length. The protein expression was evaluated using PDQuest 2-D softwareTM. The differentially expressed protein spots were identified with a MALDI-TOF mass spectrometer, and the peptide mass spectra identifications were performed using the Mascot program and by searching the Swiss-prot or NCBInr databases.Results: A total of 35 proteins were detected in SCC.17 proteins were up-regulated and 18 proteins weredownregulated.Among the proteins that were identified, 12 proteins (pigment epithelium derived factor, annexin A2 and A5, keratin 19 and 20, heat shock protein 27, smooth muscle protein 22 alpha, α-enolase, squamous cell carcinoma antigen 1 and 2, glutathione S-transferase and apolipoprotein a1) were protein previously known to be involved in tumor, and 21 proteins were newly identified in this study.Conclusion: 2-DE offers the total protein expression profiles of SCC tissues; further characterization of these differentially expressed proteins will give a chance to identify the badly needed tumor-specific diagnostic markers for SCC. (Cancer Res Treat. 2006;38:99-107)