비소세포 폐암종에서 Cyclin D1, p21 및 p53단백의 발현과 예후와의 관계

양석우,조상호,양우익,정우희,안철민,이두연 대한병리학회 1999 Journal of Pathology and Translational Medicine Vol.33 No.12

갑상선 결절에 동반된 퇴행성 낭종의 재발과 낭액내 VEGF의 관련성

조은호,박희붕,김현만<SUP>1<.SUP>,이관우<SUP>1<.SUP>,정윤석<SUP>1<.SUP>,소의영,Eun Ho Cho,M.D.,Hee Boong Park,K.D.,Hyun Man Kim,M.D.<SUP>1<.SUP>,Kwan Woo Lee,M.D.<SUP>1<.SUP>,Yun Suk Jung,M.D.<SUP>1<.SUP> and Euy Young 대한갑상선-내분비외과학회 2002 The Koreran journal of Endocrine Surgery Vol.2 No.1

Purpose: VEGF, a potent angiogenic factor, increases vascular permeability and induces the fluid accumulation in the peritoneal and pleural cavities. This study is designed to know whether the VEGF concentration are associated with the formation of cystic fluid of thyroid nodules and the results of treatment. Methods: To know whether thyroid follicular cells produce VEGF, we measured the VEGF concentration in the conditioned medium after primary culture of the normal thyroid follicular cells (NT 1.0). Thirty seven patients, who had visited Ajou University Medical Center, were divided into two groups; group A (n=19) is patients whose cystic nodules disappeared completely with 1 or 2 times fine needle aspirations. In Group B (n=18), the cystic nodules recurred rapidly and required aspiration more than 3 times. Clinical records of patients were analyzed and compared with TSH and VEGF levels in cystic fluids. Results: The VEGF concentration in basal conditioned medium were 8.2⁑1.2 ng/ml in NT 1.0 and 8.2⁑0.6 ng/ml in thyroid cancer cell line (FTC-133). NT 1.0 was not affected by TSH stimulation. Mean concentration of TSH of all cystic fluids was 4.36μIU/ml (0.13∼21.7) and there was no difference between 2 groups. Mean concentration of VEGF of all cystic fluids was 140.7 ng/ml (11.1∼688.8). The VEGF concentration (287.9⁑289.6 ng/ml) in group B was significantly higher than that (104.2⁑97.1 ng/ml) in group A (P<0.05). Conclusion: This study suggests that thyroid follicular cells produce and secrete VEGF, and VEGF is related with the accumulation of cystic fluid in degenerative cysts of thyroid. Cysts with high VEGF concentration promote rapid reaccumulation of the cystic fluid and possibly necessitate operation in selected patients. (Korean J Endocrine Surg 2002;2:25- 30)

추황배(Pyrus pyrifolia Nakai cv. Chuhwangbae) 과피로부터 1종의 Sterol과 3종의 배당체 화합물의 단리·동정

이유건(Yu Geon Lee1),조정용(Jeong-Yong Cho),이현주(Hyun Joo Lee),이용현(Yong Hyun Lee),이상현(Sang-Hyun Lee),한태호(Tae-Ho Han),김월수(Wol-Soo Kim),박근형(Keun-Hyung Park),문제학(Jae-Hak Moon) 한국식품과학회 2013 한국식품과학회지 Vol.45 No.5

본 연구에서는 배의 유용성 증명을 위한 일환으로 배의 화학성분을 분자수준에서 밝히고자 하였다. 이에 배 과피 MeOH 추출물을 용매분획하여 얻은 EtOAc-산성 분획과 EtOAc-중성분획을 대상으로 Sephadex LH-20, silica gel, 그리고 ODS colmn chromatography와 HPLC를 이용하여 정제 및 단리하였다. 그 결과, EtOAc-산성 분획과 EtOAc-중성 분획으로부터 각각 2종씩의 화합물을 단리하였다. 단리된 화합물 1-4는 1H- 및 13C-NMR 분석을 통하여 각각 (S)-(+)-2-cis-abscisic acid O-β-D-glucopyranosyl ester (화합물 1), 1-[4-O-β-D-glucopyranosyl]phenyl ethanone(piceoside, 화합물 2), β-sitosterol (화합물 3), 그리고 β-sitosteryl 3-O-β-D-glucopyranoside (화합물 4)로 동정되었다. 단리된 3종의 배당체 화합물(화합물 1, 2, 4)들은 본 연구에 의해 배로부터 처음 동정되었으며, 화합물 3은 추황배로부터 처음 동정되었다. 본 연구결과가 배 함유 성분연구는 물론 배의 기능성 해명 연구에도 추후 중요한 기초자료로 활용되길 기대한다. We isolated and identified antioxidants from acidic and neutral ethyl acetate fractions of the peel of pear (Pyrus pyrifolia N. cv. Chuhwangbae). We isolated 4 compounds from the methanol extract, by using 3 different types of column chromatography (Sephadex LH-20, silica gel, and octadecylsilane) and preparative HPLC. We identified the isolated compounds as (S)-(+)-2-cis-abscisic acid O-β-D-glucopyranosyl ester (compound 1), 1-[4-O-β-D-glucopyranosyl]phenyl ethanone (picroside, compound 2), β-sitosterol (compound 3), and β-sitosteryl 3-O-β-D-glucopyranoside (compound 4) by nuclear magnetic resonance analysis. We are the first to report the identification of compounds 1, 2, and 4 from pear.

Cellular and Histological Differences between ACL Bundles of Goat Knee

( Ho Lee1 ),( Tomoyuki Matsumoto ),( Masahiro Nozaki ),( Sheila J M Ingham ),( Freddie H Fu ) 한국조직공학·재생의학회 2008 조직공학과 재생의학 Vol.5 No.4

We aimed to investigate whether there exist the differences in the cellular properties and histological structures between AM and PL bundle of goat ACL. ACL tissues obtained from goat knees were divided to two experiments: tissue explant culture and histological assessment. AM bundle was divided into 3(proximal, middle, and distal) and PL into 2(proximal, distal) zones according to the length for AM and PL bundle. In the tissue explant culture, ACL cells demonstrated 2 different cell types which consist of spindle shaped fibroblasts and ovoid shaped fibrochondrocytes and three different cellular outgrowth patterns. The percentage of fibrochondrocytic pattern of AM bundle was significantly higher than that of PL bundle. In the section divided analysis of AM, the rate of fibrochondrocytic pattern in the mid portion was significantly lower than that in the proximal and distal portion. In the histological evaluation, we identified 3 distinguishable bundles divided by the septum, AM, PL, and intermediate( IM), and two different types of cells, fibroblasts and fibrochondrocytes, in all portions of AM and PL. The number of fibrochondrocytes stained by toluidine blue in AM bundle was larger than that in PL bundle. There was no difference in the cell density between AM and PL bundle of ACL, whereas the crimp length was longer in AM bundle compared to PL bundle. Our finding demonstrated that the goat ACL had the differences in cellular morphology, outgrowth pattern, and histological structure between AM and PL bundle of ACL.

Incidental Left Ventricular Thrombus Visualized by Cardiac Magnetic Resonance Imaging with a Normal Left Ventricular Systolic Function

Sang Mi Ro1,Sung Ho Her,Sol Mi Huo,Kuhn Park,Jong Bum Kwon,Dong Jae Lee,Hyun Jin Noh 조선대학교 의학연구원 2015 The Medical Journal of Chosun University Vol.40 No.3

Left ventricular (LV) thrombi may be caused by various conditions, particularly myocardial infarction. In most cases, LV thrombus occurs in patients with a significantly reduced ejection fraction. A LV thrombus is extremely rare in patients with normal LV function. We report a case of LV thrombus initially detected on transthoracic echocardiography and confirmed using cardiac magnetic resonance imaging in a patient with normal LV wall motion. We highlight the rarity of this condition and the usefulness of cardiac magnetic resonance imaging in the diagnosis of LV thrombus.

갑상선암 수술 중 발생하는 의도하지 않은 부갑상선 절제의 임상적 의의

김영균,김정구,이동호,이혜경<SUP>1<.SUP>,유영경,안창준,Young Gyun Kim,M.D.,Jeong Gu Kim,M.D.,Dong Ho Lee,M.D.,Hae Gyung Lee,M.D.<SUP>1<.SUP>,Young Kyoung Yoo,M.D. and Chang Joon Ahn,M.D. 대한갑상선-내분비외과학회 2006 The Koreran journal of Endocrine Surgery Vol.6 No.1

Purpose: Many surgeons intend to preserve all of the parathyroids during a thyroid operation; however, they have had the unpleasant experience of achieving unintentional parathyroidectomy. We studied the risk factors for unin-tentional parathyroidectomy and whether these unintentional parathyroidectomies caused symptomatic hypocalcemia. Methods: We conducted a retrospective review of the medical records and pathologic reports of 95 papillary carcinoma patients who underwent operations between January 1994 and December 2003. We statistically analyzed the correlation between unintentional parathyroidectomy and the risk factors such as tumor size, capsular invasion, including extra-thyroidal extension, and the operation method. Results: Of the total 95 procedures, 14 (14.7%) produced patho-logy reports stating that incidental parathyroid tissue was identified within the thyroidectomy specimen. The majority of the 14 reported cases contained only a single focus of inci-dental parathyroid tissue; however, one of these thyroidectomy specimens contained two foci of parathyroid tissue. The factors such as tumor size, capsular invasion and extensive surgery were not correlated with the prevalence of unintentional parathyroidectomy. There was no association of unintentional parathyroidectomy with postoperative hypocalcemia (P=.449).Conclusion: Unintentional parathyroidectomy is not associated with symptomatic postoperative hypocalcemia. The factors such as tumor size, capsular invasion or extensive surgery are not risk factors for unintentional parathyroidectomy. (Korean J Endocrine Surg 2006;6:17-21)

人蔘사포닌의 抗酸化作用에 대한 脂溶性 비타민의 添加效果

이향숙(Hyang Sook Lee),우순임(Soon 1m Woo),최진호(Jin Ho Choi) 한국식품영양과학회 1983 한국식품영양과학회지 Vol.12 No.2

1. DPPH에 대한 전자공여능은 vitamin A와 D가 인삼사포닌 단독보다 높아 지용성 비타민 첨가에 의한 상승효과가 인정되었다.<br/> 2. 과산화지질 생성초기의 유도기간은 vitamin A, D를 첨가했을 때 대조군이나 인삼 사포닌보다 효과적으로 유도기간을 연장시킴을 알 수 있었다.<br/> 3. in vitro에서 과산화지질 생성억제작용을 TBA value로 조사한 결과 vitamin E>A>D의 순으로 지용성 비타민의 첨가효과가 인정되었다.<br/> 4. in vivo실험에서 인삼 사포닌 및 지용성 비타민 첨가 투여에 의한 rat의 체중 및 간장, 신장의 장기중량 변화는 지용성 비타민의 첨가가 체중증가에 효과적임을 알 수 있었고, 간장은 비타민 첨가군에서 다소 중량증가가 인정되었으나 신장의 중량변화에는 큰 유의성을 발견 할 수 없었다.<br/> 5. in vivo실험에서 인삼 사포닌 및 지용성 비타민 첨가 투여에 의한 rat의 혈액에서의 과산화지질 생성억제작용은 vitamin E>D>A의 순으로 나타났고, 간장에서는 vitamin A와 E첨가군에서 효과적임이 인정되었다. This study was to demonstrate the additional effect of fat-soluble vitamins on inhibitory action of Korean ginseng (Panax ginseng C. A. Meyer) saponin to lipoperoxide formation in vitro and in vivo.<br/> The ginseng saponin and vitamins were added to the substrate of linoleic acid and incubated on a shaking water-bath at 60℃, and the inhibitory action on lipoperoxide formation was examined by measuring the TBA value (532㎚), POV (500㎚) and EDA (electorn donating ability to a, a-diphenyl-β-picrylhydrazyl a 525㎚) for in vitro.<br/> The ginseng saponin and vitamins were administered to Sprague-Dawley rats (♂, 100~120g) orally, and the inhibitory effect on lipoperoxide formation was examined by measuring the TBA value in vivo.<br/> EDA to DPPH of ginseng saponin added with vitamin A and D were higher than that of ginseng saponin only. Ginseng saponin added with vitamin A and D inhibited strongly lipoperoxide formation of initial step by extension of induction period in vitro.<br/> Fat-soluble vitamins such as vitamin E and A were approved the additional effect on inhibitory action of lipoperoxide formation in vitro. The additional effect of fat-soluble vitamins to ginseng saponin for inhibitory action of lipoperoxide formation was effective vitamin E and D for blood, vitamin A and E for liver in vivo.

Expression analysis of the fpr (ferredoxin-NADP^(+) reductase) gene in Pseudomonas putida KT2440

Lee, Yun-Ho,Samuel Pe?a-Llopis ^(b,1),Kang, Yoon-Suk,Shin, Hyeon-Dong,Bruce Demple,Eugene L. Madsen,Jeon, Che-Ok,Park, Woo-Jun Plant molecular biology and biotechnology research 2005 Plant molecular biology and biotechnology research Vol.2005 No.

The ferredoxin-NADP^(+) reductase (fpr) participates in cellular defense against oxidative damage. The fpr expression in Pseudomonas putida KT2440 is induced by oxidative and osmotic stresses. FinR, a LysR-type transcriptional factor near the fpr gene in the P. putida KT2440 genome, is required for induction of the fpr under both conditions. We have shown that the fpr and finR gene products can counteract the effects of oxidative and osmotic stresses. Interestingly, FinR-independent expression occurs either during a long period of incubation with paraquat or with high concentrations of oxidative stress agent. This result indicates that there may be additional regulators present in the P. putida KT2440 genome. In contrast to in vivo expression kinetics of fpr from the plant pethogen, Pseudomonas syringae, the fpr gene from P. putida KT2440 exhibited unusually prolonged expression after oxidative stress. Transcriptional fusion and Northern blot analysis studies indicated that the FinR is negatively autoregulated. Expression of the fpr promoter was higher in minimal media than in rich media during exponential phase growth. Consistent with this result. the fpr and finR mutants had a long lag phase in minimal media in contrast to wild-type growth characteristics. Antioxidants such as ascorbate could increase the growth rate of all tested strains in minimal media. This result confirmed that P. putida KT2440 experienced more oxidative stress during exponential growth in minimal media than in rich media. Endogenous promoter activity of the fpr gene is much higher during exponential growth than during stationary growth. These findings demonstrate new relationships between fpr, finR, and the physiology of oxidative stress in P. putida KT2440. ⓒ 2005 Elsevier Inc. All rights reserved.

Sarcomatoid Carcinoma Arising from Mature Cystic Teratoma

Ho-Chang Lee1, Seung-Myoung Son, Yong-Moon Lee, Ji Hae Koo, Song-Yi Choi, Ok-Jun Lee, Eun-Hwan Jeong 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.2

Malignant transformation of mature cystic teratoma (MCT) is rare. Sarcomatoid carcinoma is a neoplasm comprising malignant mesenchymal cells and a conventional carcinomatous area. Here, we report on a case of sarcomatoid carcinoma arising from an MCT in the left ovary of a 45-year-old female. A unilocular cyst consistent with MCT was observed; however, a nodule within the cyst was confirmed from the resected ovary. Microscopically, the nodule showed both squamous cell carcinoma and pleomorphic sarcomatous components admixing with each other. Lining epithelial cells at the periphery of the main tumor showed squamous metaplasia. When a sarcomatous component is observed in the ovary tumor, it is important to find a squamous cell component, either benign or malignant.

최신판례분석 : 학교생활기록부 작성에 관한 사무의 법적 성질 - 대법원 2014. 2. 27. 선고 2012추183 판결 -

이호용1 ( Lee Ho Yong ) 법조협회 2017 法曹 Vol.66 No.1

2012년 학교폭력 가해 학생에 대한 조치사항을 학교생활기록부에 기재하라는 교육부의 조치는 5년이 지난 지금까지도 여전히 논쟁의 대상이 되고 있다. 그 후 4번의 법률개정안이 발의 되었으며, 조금이라도 불이익한 사항을 기재하지 않으려는 가해 학생측의 학교폭력자치위원회의 처분에 대한 재심청구 건은 1,000건에 육박하고, 학교폭력대책자치위원회 처분 관련 소송은 연간 100건을 넘어서고 있다(2015년 기준). 이 정책과 법제에 대한 재검토의 목소리가 더욱 높아졌다. 학교폭력 가해 학생에 대한 조치사항을 학교생활기록부에 기재하라는 교육부의 시정명령 등과 이에 불복한 경기도교육감의 취소소송을 다룬 이 판례에서는 학교생활기록의 작성에 관한 교육감의 지도ㆍ감독 사무는 국민 전체의 이익을 위하여 통일적으로 처리되어야 하는 사무로서 교육감에게 위임된 국가사무에 해당한다고 하면서 지방자치법 제169조 제2항은 자치사무에 관한 명령이나 처분의 취소 또는 정지에 대해서만 소를 제기할 수 있으므로 학교폭력 가해학생 조치사항을 학교생활기록부에 기재하도록 하기 위한 교육부장관의 시정명령이나 직권 취소처분을 취소해 달라는 소송은 허용되지 않는다고 하여 소를 각하하였다. 따라서 쟁점은 학교생활기록의 작성에 관한 교육감의 지도ㆍ감독 사무가 국가로부터 위임된 사무인가 아니면 자치사무인가에 있다. 판례는 학교생활기록은 시ㆍ도를 달리하여 또는 국립학교와 공립ㆍ사립학교를 달리하여 전출하는 경우에 통일적 관리가 필요하고 학교생활기록을 상급학교 입학전형에 반영될뿐만 아니라 교육부가 지도ㆍ감독하는 대학교의 입학전형자료로 활용되므로 국민전체의 이익을 위하여 통일적으로 처리되어야 할 사무이고 따라서 이에 대한 지도ㆍ감독사무도 통일적으로 처리되어야 할 사무이므로 국가사무라고 결론 짓고 있다. 이 판례의 문제점은 지방자치단체 사무를 구분함에 있어서는 그 사무의 성질이 아니라 그 사무가 활용되는 상황을 기준으로 하였다는 점이다. 물론 사무의 성질이 지방자치단체의 사무를 구분하는 획일적이고 절대적인 기준은 아니나, 사회가 처한 상황에 따라 사무구분을 달리할 때에는 그에 따른 합리적 이유로서 설득될 수 있어야 한다. 또한 이 논문에서는 판례에서는 다루지 아니한 쟁점으로서 학교폭력 가해학생에 대한 조치사항을 학교생활기록부에 기재하도록 한 근거를 법령이 아닌 교육부 훈령인 「학교생활기록 작성 및 관리지침」에 두고 있는 것의 법적 정당성과 학교 폭력의 근절이라는 행정목적을 달성하기 위한 수단으로서 학교 폭력 가해 사실을 학교생활기록부에 의무적으로 기록하도록 하는 것의 비례원칙에 위반 여부에 대해서도 함께 검토하였다. The Ministry of Education`s action in 2012 to include measures on school violence students in school life records is still controversial five years later(2017). Thereafter, four legal amendments were proposed, the number of appeals for the dismissal of the School Violence Authority Committee is close to 1,000, and the number of lawsuits related to the dismissal of the School Violence Autonomy Committee exceeds 100 cases per year(as of 2015). The voice of the review of the policy and legislation has become even more vocal. In this case, it was decided as follows. First, the supervision of the superintendent of the school record is equivalent to the national affairs entrusted to the superintendent, which should be handled uniformly for the benefit of the whole people. Second, Article 169 Paragraph 2 of the Local Autonomy Law can only be filed for cancellation or suspension of order or disposition of self-government affairs. Therefore, No lawsuit is required to cancel the order of the Minister of Education or the order for cancellation of the delegation. Therefore, this lawsuit is dismissed. The issue of this case is whether the superintendent`s supervision of school life records is delegated from the state or self-governing. The criterion that determines whether it is a national affairs or an autonomous affairs is in the nature of office work, But this case was based on the situation in which that affair was utilized, not the nature of office work. This is the problem of this case. Only the nature of office work is not a decision criterion, if want to follow exceptions that are not principles, you should give a reasonable reason, but this case did not. And in this paper, it is contrary to statutory reservation principle to prescribe violation of school violence based on the “Guidelines for the preparation and management of school life records(Ministry of Education Directive)” it also contradicts the proportional principle.