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Lim, Heon Man 충남대학교 기초과학연구소 1995 연구논문집 Vol.15 No.-
The Hin recombinase exists as a homodimer in solution and binds to its recombination site (hix) as a dimer (Glasgow, A. C., Bruist, M. F., and Simon M. I. (1989) J. Biol. Chem. 264, 10072-10082). Previous mutational and structural studies of related proteins suggested the location of a putative dimerization domain. In order to probe the function of this region of the protein, cysteine residuces were introduced at each of the seven positions comprising the domain. Proteins containing cysteine substitutions at positions 101 and 104 were able to form disulfide bonds spontaneously in crude extracts. M101C showed wild type inversion activity only if the cysteine residue was not engaged in a disulfide bond. This mutant, which is cross-linked by a disulfide bond at the dimeric interface, was found to be defective in the DNA cleavage step during inversion. H107C displayed the wild type DNA cleavage activity, even though its DNA binding activity was not detected by three different binding assays. This suggests that it maintains specificity for DNA binding but dissociates rapidly after binding. The remaining inversion-defective mutants fell into two groups. One group lost its capacity to specifically bind to DNA (G102C, F105C, and F106C), and the other group (R103C and F104C), while able to bind to DNA, was defective in subsequent steps of the inversion reaction. Characteristics of these mutants led me to postulate that movement of subunits at the dimerization interface is critical during DNA binding and during formation of the invertasome, which is the recombination structure. Furthermore, the relative position of subunits within the dimer may be important for maintaining stable DNA binding.
병역정책의 형평성 분석모형의 정립과 그 적용에 관한 연구
임헌만(Lim Heon-Man) 서울행정학회 2003 한국사회와 행정연구 Vol.14 No.1
The equity of the public policy has recently emerged an important issue in the field of policies. The problem in the equity of the public policy is that the public policy target person(PPTP) don't accept straightly the equity of the public policy even if the public policy is absolutely equitable. The PPTP evaluates the equity of the public policy by scrutinizing his inputs-distributions ratio(distribution/input) and other person's one. This study is interested in the equity of the military system of R.O.K.. So this study applies the equity theory to this system. In conclusion, the common military service obligor in the present military service system is likely to have a perception of nonfavourable equity by comparison with others because there are many special cases in the present military service system. Therefore, the Office of Manpower Administration has to listen to the protest related to the equity of the military service, reappraise the present military service system, and make efforts to secure compliance with this system.
Multicriteria Optimization of Spindle Units
Lim Sang-Heon,Lee Choon-Man,Zverev Igor Aexeevich Korean Society for Precision Engineering 2006 International Journal of Precision Engineering and Vol.7 No.4
The quality of precision spindle units (S/Us) running on rolling bearings depends strongly on their structural parameters, such as the configuration and geometry of the S/U elements and bearing preloads. When S/Us are designed, their parameters should be optimized to improve the performance characteristics. However, it is practically impossible to state perfectly a general criterion function for S/U quality. Therefore, we propose to use a multicriteria optimization based on the parameter space investigation (PSI) method We demonstrate the efficiency of the proposed method using the optimization results of high-speed S/Us.
Lim Sang-Heon,Lee Choon-Man,Chung Won Jee Korean Society for Precision Engineering 2006 International Journal of Precision Engineering and Vol.7 No.1
Cutting by a high speed laser cutting machine is one of most effective technologies to improve productivity. This paper has presented the cutting characteristics and optimal cutting conditions in a high speed feeding type laser cutting machine by using Tacuchi method in the design of experiment. An L9(34) orthogonal array is adopted to study the effect of adjustment parameters. The adjustment parameters consist of cutting speed, laser power, laser output duty and assistant gas pressure. The surface roughness of sheet metal is regarded as a quality feature. Analysis of variance is performed in order to evaluate the effect of adjustment parameters on the quality feature of laser cutting process.
지방자치제하의 정치와 행정간 관계에 관한 연구 : 군집분석을 통한 정치 - 행정관계의 유형화와 유형별 특성 분석
임헌만 한국정책학회 1999 韓國政策學會報 Vol.8 No.3
본 연구는 정치와 행정간 관계에 관한 행정학계의 오랜 주제를 우리 나라 지방자치현장에서 실증주의적 방법론에 입각하여 분석하였다. 본 연구는 제5대 지방의회 기간 동안(1995.7∼1998.6) 전국 230개 기초자치단체의 지방의회와 집행기관의 실제 역할수행에 관한 방대한 경험적 자료를 이용하여 정치-행정관계를 5개로 유형화(평균형, 지방의회우위형, 상호침투형, 상호자제형, 집행기관우위형)하였고, 이들 유형과 관련요인과의 관계에 대한 통계적 검증을 통해 각 유형별 특성을 밝히고자 하였다. 본 연구의 핵심적 분석결과와 그 시사점은 첫째, 재정자립도가 큰 자치단체들에서 지방의회우위형 및 상호침투형의 정치-행정관계가 많이 나타났는 바, 이것은 지방의회(또는 지방의원)의 역할제고를 위해 합리적인 재원배분을 통한 지방재정자립도의 제고가 필요함을 시사해준다. 둘째, 야당단체장하에서 지방의회우위형이 그리고 여당단체장 및 무소속단체장하에서 집행기관우위형의 정치-행정관계가 많이 나타났는 바, 이것은 기초의회의원들의 선거시 정당표방을 허용하지 않는다 하더라도 정당이 지방자치과정에 영향을 미치고 있음을 말해주는 것으로 해석되며, 기초자치 수준에서의 정당참여에 대한 재검토 필요성을 제기한다. 셋째, 지방의원들의 학력과 재선의원비율이 높은 자치단체들에서 지방의회우위형의 정치-행정관계가 많이 나타났는 바, 이것은 지역내 우수한 인사들의 정치참여를 유도하는 정책적 노력이 필요하다고 판단된다.