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녹차 및 매실 추출물이 N-Nitrosodimethylamine의 생성에 미치는 영향
최선영,정미자,신정혜,김행자,성낙주 한국식품영양학회 2002 韓國食品營養學會誌 Vol.15 No.1
녹차 및 매실추출물이 아질산염 소거, 전자 공여능 및 NDMA 생성 억제에 미치는 영향을 분석하고, 질산염과 아민이 풍부한 식단(CW)에 인공타액과 위액을 이용한 "simulated digestion" 에서 확인하였다. 녹차와 매실 추출물의 아질산염 소거작용을 pH 1.0, 4.2 및 6.0에서 실험 결과, pH가 낮을수록, 시료 첨가량이 많을수록 효과적이었으며 pH 1.2에서 녹차 추출물 0.5ml 첨가시킬 경우 99.6%의 높은 소거작용을 나타내었고, 매실 추출물 3ml 첨가시 77.2%의 소거작용을 나타내었다. 녹차 및 매실 추출물의 전자공여 작용은 각각 70.6%, 75.1%로 높게 나타났다. 녹차와 매실 추출물의 NDMA 생성억제 효과는 pH 1.2 에서 가장 뛰어났고, 시료량이 증가할수록 억제효과가 높아 3ml 첨가시에 82.1%와 73.2%의 억제효과를 나타내었다. 실험식이에 녹차 및 매실 추출물의 첨가량을 달리하여 인공소화시킬 때 NDMA 생성억제 효과는 시료 첨가량에 비례하여 각각 20ml 첨가시 녹차 추출물은 57.9%, 매실 추출물은 48.4%의 NDMA 생성억제 효과를 나타내었다. The effects of nitrite scavenging, electron donating and N-nitrosodimethylamine (NDMA) formation in vitro and green tea (Camellia sinensis) and Maesil (prunus mume) were studied. The green tea and Maesil extracts were tested for their nitrite-scavenging effect under the different pH conditions such as pH 1.2, 4.2 and 6.0. The effects of nitrite-scavenging in all concentrations were diminished in the alkali condition, whiled its effects in the acidic condition of pH 1.2 were reached of more than 99.0% by adding above 0.5ml of green tea extract. And also, nitrite-scavenging effect by adding 3ml of Maesil extract was about 77.0%. The electron donating ability (EDA) of green tea and Maesil extracts was 70.6%, 75.1%, respectively. The formation of NDMA was very effectiveness which was inhibited 82.1%, 73.2% at reaction mixture of pH 2.5 adding 3ml of above extracts, respectively. The ground CW, TW1 and TW2 (refer to Table 1) diets were incubated with 10ml simulated saliva and 40 ml gastric juice at 37℃ for 2hrs. NDMA formation was inhibited at all levels of green tea and Measil extracts.
Kim Haeng Jun,Park Jong-Chan,Jung Keum Sim,Kim Jiyeong,Jang Ji Sung,Kwon Sunghoon,Byun Min Soo,Yi Dahyun,Byeon Gihwan,Jung Gijung,Kim Yu Kyeong,Lee Dong Young,Han Sun-Ho,Mook-Jung Inhee 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-
Alzheimer’s disease (AD) is the leading cause of dementia, and many studies have focused on finding effective blood biomarkers for the accurate diagnosis of this disease. Predicting cerebral amyloid deposition is considered the key for AD diagnosis because a cerebral amyloid deposition is the hallmark of AD pathogenesis. Previously, blood biomarkers were discovered to predict cerebral amyloid deposition, and further efforts have been made to increase their sensitivity and specificity. In this study, we analyzed blood-test factors (BTFs) that can be commonly measured in medical health check-ups from 149 participants with cognitively normal, 87 patients with mild cognitive impairment, and 64 patients with clinically diagnosed AD dementia with brain amyloid imaging data available. We demonstrated that four factors among regular health check-up blood tests, cortisol, triglyceride/high-density lipoprotein cholesterol ratio, alanine aminotransferase, and free triiodothyronine, showed either a significant difference by or correlation with cerebral amyloid deposition. Furthermore, we made a prediction model for Pittsburgh compound B-positron emission tomography positivity, using BTFs and the previously discovered blood biomarkers, the QPLEX TM Alz plus assay kit biomarker panel, and the area under the curve was significantly increased up to 0.845% with 69.4% sensitivity and 90.6% specificity. These results show that BTFs could be used as co-biomarkers and that a highly advanced prediction model for amyloid plaque deposition could be achieved by the combinational use of diverse biomarkers.
정행선 ( Haeng Sun Jung ),이수현 ( Su Hyon Lee ),류양환 ( Yang Hwan Ryu ),정경미 ( Kyoung Mi Jung ),김채욱 ( Chae Wook Kim ),임경민 ( Kyung Min Lim ),손영숙 ( Young Sook Son ) 한국동물실험대체법학회 2009 동물실험대체법학회지 Vol.3 No.1
Alternative methods to the Draize eye irritation test, such as the BCOP, HET-CAM, ICE, and IRE, are used to evaluate the ocular irritation potential of cosmetic, livelihood articles or industrial chemicals. In order to improve the sensitivity and specificity of alternative eye irritation test, we developed a three-dimensional human corneal model that uses normal human corneal epithelial cells. Our corneal tissue model consists of normal human corneal epithelial cells on cell culture inserts at the air liquid interface, differentiating to form a stratified, squamous epithelium similar to that found in the human cornea, exhibiting in vivo like morphological and histological characteristics. In this study, two laboratories have tested 20 reference chemicals using the same study protocol. The results were compared to in vivo data as well as previously published data obtained in the other three-dimensional corneal model test. Although an overall accuracy of 85% was obtained (sensitivity=100% and specificity=67%), further experiments are required to confirm and validate these preliminary results.
정행선 ( Haeng Sun Jung ),이수현 ( Su Hyon Lee ),류양환 ( Yang Hwan Ryu ),정경미 ( Kyoung Mi Jung ),김채욱 ( Chae Wook Kim ),임경민 ( Kyung Min Lim ),손영숙 ( Young Sook Son ) 한국동물실험대체법학회 2009 동물실험대체법학회지 Vol.3 No.1
Alternative methods to the Draize eye irritation test, such as the BCOP, HET-CAM, ICE, and IRE, are used to evaluate the ocular irritation potential of cosmetic, livelihood articles or industrial chemicals. In order to improve the sensitivity and specificity of alternative eye irritation test, we developed a three-dimensional human corneal model that uses normal human corneal epithelial cells. Our corneal tissue model consists of normal human corneal epithelial cells on cell culture inserts at the air liquid interface, differentiating to form a stratified, squamous epithelium similar to that found in the human cornea, exhibiting in vivo like morphological and histological characteristics. In this study, two laboratories have tested 20 reference chemicals using the same study protocol. The results were compared to in vivo data as well as previously published data obtained in the other three-dimensional corneal model test. Although an overall accuracy of 85% was obtained (sensitivity=100% and specificity=67%), further experiments are required to confirm and validate these preliminary results.