PCR을 이용한 국내시장에 유통중인 유전자재조합 콩 및 가공식품의 모니터링

김묘영,김재환,김현중,박선희,우건조,김해영 한국응용생명화학회 2003 Applied Biological Chemistry (Appl Biol Chem) Vol.46 No.4

본 연구에서는 PCR을 이용하여 국내시장에 유통중인 원료콩과 가공식품에 epsps 또는 pat 유전자가 삽입된 유전자재조합 콩(GMS)의 사용여부를 모니터링하였다. 이러한 GMS의 검출을 위해 3쌍의 primer set을 제작하였고, 각각의 primer들은 GMS에 삽입된 유전자와 특이적으로 반응하여 PCR산물을 생성하였다. 2001년 표시제가 시행되기 이전에 생산된 총 가공식품과 이후의 제품에 대해 각각 모니터링을 수행하였으며, 표시제 이전에 생산된 제품의 경우 대부분의 미국산 원료에서 esps가 삽입된 GMS가 검출되었으나, 표시제 이후에는 검출되지 않았다. A method using PCR was developed for the monitoring of genetically modified soybean (GMS) and GMS derived foods utilized in the market. We designed 3 pairs of specific oligonucleotide primers based on epsps and pat inserted in GMS and ferritin gene as internal standards. Template DNAs isolated from soybean and processed foods were used for multiplex PCR with 3 primer sets. PCR, used with specific primer sets for GMS detection, showed the amplified DNA fragments with GMS template DNA. In this study, GMS containing epsps was detected from soy processed foods manufactured before GM food labeling system, however, GMS containing epsps or pat was not detected from soy processed foods manufactured after GM food labeling system.

알루미늄 합금과 고장력 강판 접합을 위한 헬리컬 SPR의 단조공정 설계

김원영, 김동범, 김기호, 김도훈, 이인환, 조해용 충북대학교 산업과학기술연구소 2013 산업과학기술연구 논문집 Vol.27 No.2

A self-piercing rivet(SPR) is a mechanical component for joining dissimilar materials. It is used for joining dissimilar material sheets such as aluminum alloy and steel. The self-piercing rivet directly pierces into sheets without drilling sheets beforehand unlike conventional rivets. However, the self-piercing rivet occurs buckling when piercing high strength steel. Therefore, a helical SPR which can pierce high strength steel sheet was designed. In this study, forging process was designed for manufacturing the helical SPR and simulated by using commercial FEM code DEFORM-3D. In the simulation of forging process for manufacturing rivet, process sequence, formability, forging load were investigated. The suitable forging process could be designed by comparisons of simulated results. Experimental trials have been carried out for same conditions as simulated ones. The simulated results agreed with the experimental ones. So, the analytic approach in this thesis is useful to save cost and time in the forging industry

개원의의 진료의뢰시 기대요인 및 만족도에 관한 연구 : 협력병원체제 여부를 중심으로

김동일,김해준,윤석준,문영배 한국의료QA학회 2002 한국의료질향상학회지 Vol.9 No.2

Background : This research, as a part of improving management process of college hospital in this rapidly changing circumstances, is searching for the actual state of private clinics referring patients to referral center including college hospitals. Methods : This research examined the sense of satisfaction and requesting degree in using referral center and by analyzing the correlation and differences among factors such as primary factors regarded as selecting ones in referring patients to a referral center in college hospital and expecting factors expected to be supported to patients and cooperative hospitals. Results : The main researched results are as follows. First, as for the primary factors, the differences between cooperative and uncooperative hospitals revealed the fact that choosing target hospital in accordance with cooperative relation and convenient process in referring patients are important. Second, satisfaction rates, analyzed from the paired t-test revealed kindness and convenience as the top priorities, while sending patients back again and benefit of supporting such as the use of facilities were revealed as the least satisfactory aspects, despite the fact that they should be fulfilled through actual service. Conclusion : The recognition of the practitioner is examined and analyzed in this research by examining primary factors in selecting college hospitals, expecting factors expected to be supported to patients and satisfaction degree, which are expected to be used as basic materials for the development of referral center of college hospitals.

구순구개열 환아에서의 치조골이식

조해성,박재홍,김광철,최성철,이긍호,최영철 대한소아치과학회 2007 大韓小兒齒科學會誌 Vol.34 No.1

치조파열 및 구개열과 같은 선천성 기형은 이환된 환자에게 기능적, 심미적으로 많은 문제점을 야기하므로 정상으로 회복시켜 주는 것은 중요한 문제이다. 장기간 방치될 경우 영양장애, 구강위생 불량, 호흡기 간염, 언어 장애, 악안면 변형, 그리고 정신적인 문제 등이 복합적으로 발생할 수 있다. 따라서 구순구개열 환자의 치료는 여러 전문 치료 분야의 복합적인 접근이 필요하다. 골이식은 구순구개열 환자에 있어 중요한 치과치료 단계이다. 치아의 맹출과 치열의 안정화를 위해 치열궁은 골격손이 없이 완전해져야 한다. 또한 치열궁의 파열이 있는 부위로는 정상적인 교정적 치아이동이 곤란하다. 따라서 구순구개열 환자에 있어 골이식은 광범위하게 적용되고 있는 외과적 술식이다. 치조골을 이식함으로써 치조열은 안정화되고, 견치 또는 절치가 이식부위로 이동할 수 있게 된다. 그리고 골 이식 후, 교정을 통해 치조열 부위의 공간을 폐쇄함으로써 보철 치료 없이 치열을 재형성할 수 있다. 골이식술에는 다양한 이식재료가 사용되었다. 자가골을 이식할 경우 장골이 가장 선호되며, 그 밖에 경골, 늑골, 두개골, 하악골을 이용하기도 한다. 그리고 골이식은 골이식 시기에 따라 일차골이식, 조기 이차골이식, 이차골이식, 만기 이차골이식으로 구분할 수 있다. 이차골이식은 혼합치열기 말경에 시행되는 것을 말하며 가장 좋은 것으로 받아들여지고 있다. 영구 견치가 맹출되기 전에 이차골이식을 시행하면 인접치아의 맹출과 보존에 도움이 된다. 본 증례에서는 치조구개파열 환자의 장골에서 골을 채취하여 골 이식을 시행한 후, 골이 채워진 이식 부위에 인접 치아가 성공적으로 이동하거나 치축이 개선되고 교정치료를 통해 치열궁 배열의 만족스러운 결과를 얻었기에 이를 보고하는 바이다 Cleft lip and palate are congenital craniofacial malformation. Reconstruction of dental arch in patient with alveolo-palatal clefts is very important, because they have many problems in functions and esthetics. Malnutrition, poor oral hygiene, respiratory infections, speech malfunctions, maxillofacial deformity, and psychological problems may be occured without proper treatment during the long period of management of the cleft lip and palate. So the treatment should be managed with a multidisciplinary approach. Bone grafting is a consequential step in the dental rehabilitation of the cleft lip and palate patient. A complete alveolar arch should be achieved of the teeth to erupt in and to form a stable dentition. And the presence of the cleft complicate the orthodontic treatment. Therefore bone grafting in patients with cleft lip and palate is a widely adopted surgical procedure. Grafted bone stabilizes the alveolar process and allows the canine or incisor to move into the graft site. After the bone grafting, orthodontic closure of the maxillary arch has become a common practice for achieving dental reconstruction without any prosthodontic treatment. Various grafting materials have been used in alveolar clefts. Iliac bone is most widely fovoured, but tibia, rib, cranial bone, mandible have also been used. And according to its time of occurrence, the bone graft may be divided into primary, early secondary, secondary, late secondary. Bone grafting is called secondary when performed later, at the end of the mixed dentition. It is the most accepted procedure and has become part of treatment of protocol. A secondary bone graft is performed preferably before the eruption of the permanent canine in order to provide adequate periodontal support for the eruption and preservation of the teeth adjacent to the cleft. In this report, we report here on a patient with unilateral cleft lip and palate, who underwent iliac bone graft. The cleft was fully obliterated by grafted bone in the region of the alveolar process. The presence of bone permitted physiologic tooth movement and the orthodontic movement of adjacent tooth into the former cleft area. Satisfactory arch alignment could be achieved in by subsequent orthodontic treatment.

새고막을 이용한 분말조미료 가공

오영열,강동수,김해섭,김경은,최옥수,배태진 여수대학교 산업기술지역개발연구소 2000 産業基術硏究所 論文集 Vol.9 No.-

Conditions for processing dried condiments with bloody clam were investigated. The enzymatic hydrolysis for 1 hour was more profitable than hydrothermal extraction to develop flavoring matters from bloody clam. As a result of omission tests, nucleotides were predominated in the taste compounds of bloody clam hydrolysates rather than free amino acids, and the contribution of nucleotides and free amino acids to the taste of bloody clam hydrolysates was remarkable. The major flavoring components of bloody clam hydrolysates were free amino acids and oligopeptides below 500 dalton. When bloody clam hydrolysates were separated with membrane(molecular weight cutoff 500 dalton) for recovering flavor, recovering yields of amino type nitrogen were 92.4%. Proximate compositions of dried condiments prepared with protease hydrolyzed bloody clam were moisture content 3.6%, total nitrogen were 71.2% amino nitrogen were 47.8%. And drying yield, solubility and absorption rates at Aw 0.88 were 21.4%. 86.3% and 6.6%, respectively.

民族主義的 側面에서 본 韓國文學 : 日帝治下의 文學을 中心으로 Especially during the Japanese Rule of Korea

梁重海,金時泰,金永和 제주대학교 1973 논문집 Vol.5 No.-

How national consciousness is reflected in Korean literature during the Japanese rule of Korea can be a matter of our concern, partly because national consciousness and nationalism embedded in literary works were one of our issues of that period, and partly because it is significant to fathom the historical import of such literature and explain its present implications. In this article, main Colonial literary works with deep layers of national consciousness and nationalism in them are looked into in three separate genres:(1) poetry, (2) fiction, (3) criticism. In poetry, national self-esteem and resistance to Japanese imperialism were urged suggestively and metaphorically in the form of expressions peculiar to poetry. In fiction, the first phase of the resistance characterized by negative enlightenment eventually grew into the phase of positive, though suggestive, manifestation and description of the spirit of resistance. In literary criticism, first, most critics analyzed the discord and friction between nationalism and socialism by inquiring into the process through which these two ideologies were introduced into literature and by surveying the scope of literary theories based on the two ideas. Secondly, they tried to find the historical import and the sphere of nationalistic literary theories by analyzing the reflection of such theories in Korean society described in literary works, and by analyzing the contribution of such theories to the formation of spiritual structure of Korean literature. Such literature tells us that literature can not be separated from realities of the times and that it abstracts and reorganizes the notions of the age. In that respect, we can assert that the Koreans under the Japanese rule criticized, denied, and resisted the Colonial age instead of accepting it tolerantly.

사람 Ferritin 유전자를 이용한 담배의 형질전환

이주안,김형석,임채완,김해영,박영두 경희대학교 생명자원과학연구원 2002 硏究論文集 Vol.22 No.-

사람 H-chain ferritin 유전자를 Agrobcterium-mediated transformation 방법으로 담배(Nicotiana tabaccum L)에 전이시켰다. 이를 위하여 사람 H-chain ferritin 유전자를 C_(S)VMV promotor와 NOS terminator를 운반하는 plant binary vector, pILTAB 357에 도입하였다. pILTAB 357를 포함하는 Agrobcterium tumefaciens LBA4404로 담배 잎 절편체를 감염시킨 후 형질전환 신초는 MS배지에 1 5 ㎎/L BA, 50 ㎎/L kanamycin 그리고 200 ㎎/L cefotaxim이 첨가된 1차 선발배지에서 유도하였다. 유도된 신초는 MS배지에 100 ㎎/L kanamycin과 200 ㎎/L cefotaxim이 포함된 2차 선발배지에서 뿌리를 유기하였다. 뿌리가 형성된 재분화 개체는 온실에서 재배하였으며 자가수분하여 종자를 수확하였다. 사람 H-chain ferritin유전자의 담배 genome내로의 전이여부를 H-chain ferritin 유전자내의 primer를 이용하여 PCR 방법으로 확인한 바 형질전환 개체에서 예상했던 560bp 단편을 볼 수 있었으나 형질전환을 시키지 않은 식물체에서는 PCR 산물을 확인할 수 없었다. Kanamycin 저항성 분석은 전이유전자의 후대로의 안정적인 유전을 보여 주었으며 저항성 종자들은 homozygous개체를 확보하기 위해 온실에서 재배 중에 있다. A human H-chain ferritin gene was introduced into tobacco (Nicotiana tabaccum L.) plants by Agrobcterium-mediated transformation. To introduce this gene, human ferritin gene was cloned into plant binary vector, pILTAB 357, horboring CsVMV promotor and nopalin synthase terminator (3' NOS). After infection with Agrobacterium tumefaciens LBA4404 containing pILTAB 357, transformed shoots were induced from first selection media (MS media + 1.5 ㎎/L BA + 50 ㎎/L kanamycin + 200 ㎎/L^(1) cefotaxim). Induced shoots were rooted in second selection media (MS media + 100 ㎎/L kanamycin + 200 ㎎/L cefotaxim). Rooted shoots were cultivated, selfed, and seeds were harvested in the green house. To confirm the transfer of the human H-chain ferritin gene in the genome of tobacco plants, PCR was conducted using specific primers of the H-chain femritin gene. A PCR fragment of 560 bp was obtained from each transformed plants, which is the predicted size of the H-cham ferritin gene but not from nontransformed plants. Kanamycin resistance assay showed that transgenes were stably inherited to next generation in all lines. These resistance seeds from each line were cultivated in the green house to obtain homozygous lines for further study.

UR에 대응한 지역농업의 발전방안 : 시설원예농업을 중심으로 With an Emphasis on the Greenhouse Horticulture Farming

배흥규,김진수,이영기,정순재,정해진,이상학 東亞大學校 1995 東亞論叢 Vol.32 No.-

The production of greenhouse horticultural products in the east region of Kyungnam province is now increasing, though the productions of other argicultural sectors have been rapidly decreasing. However, as the agricultural market lies under the big pressure of the WTO system having a goal of free trade of all commodities, greenhouse horticultural farming in the region also meets with many new problems. To identify problems and prepare development strategies of the greenhouse horticultural farming for the easten Kyungnam region, 10 villages in Kimhae, Yangsan and Miryang areas were selected as sample areas and 204 greenhouse vegetable or floriculture farms were surveyed using prepared questionaires during March-April 1995. From the analysis of the regional survey results, the following proposals are suggested for the development of regional horticultural farming : 1) There were still old-fashioned greenhouse facilities such as tunnel-type vynil house, no heating system and man-power watering. Greenhouse facilities should be modernized and automated in order to overcome the severe labour shortage problem constraining the increase of the greenhouse farming size and the enhance the degree of the commodity value by uniform quality control and year-round production. The money for the modernization and automation of greehouse facilities ned to be supported by the central government, because farmers lack the ability to fund the investment. 2) Not many farmers visited agricultural extension officers to obtain cultivation techniques of greehouse horticulture farming, because extension officers lack the manpower to help solving the greehouse farming problems. Agricultural system should be adjusted to the changing agricultural production structure. Extension manpower specialized in greehouse horticultural farming need be increased and located in the regional extension offices. 3) The major reason of farmer's pessimistic prospect for the future of greegouse horticulture was the possibility of overproduction of some horticultural products. To avoid the overproduction, new entrants to the greenhouse horticultural farming of products already culticated extensively should be limited and the government support must be given to the existing greenhouse farms and major production areas. 4) Local goverment, associated agencies, and farmers should work hard cooperatively to increase the export of horticultural products especially to Japan, the largest importer of agricultural products in the world. Local governments and associated agencies can provide informations related to the export market and provide the fund for the facilities of standardization of export products. Farmers must observe the export contracts, even though the domestic price exceeds the export price. 5) Collective marketing activities of greenhouse products were found to be mainly limited to transportation and handling of sales revenue. Central and local government should support the formation and collective activities of cooperatives of specilaized producers to enhance the bargaining power of farmers. 6) The role of local government should be increased. They should actively participate in the orientation of regional greenhouse farming and play an important role in the marketing of regional greenhouse products.

Ⅲ-Ⅴ족 반도체의 연구 : GaAs의 합성, 단결성 및 특성 Crystal Growth and Characterization of GaAs

이형재,김영기,신영진,박해용,정양준,최춘태,김병호,김진성 全北大學校 基礎科學硏究所 1982 基礎科學 Vol.5 No.1

Temperature of 618 and 1250℃ at As and Ga sities respectively in the horizontal Bridgman furnace and growht speed of 0.7cm/h were found to be optimal for the production of single crystalline GaAs. Self-seeded and unintentionally doped GaAs crystals showd n-type characteristics with Hall mobillity of ∼0.75㎡/V-sec and carrier density of ∼10^19 m^-3 when the mesurements were made right after the growth. About three month later it was found that the same samples were converted to p-type GaAs. The analysis on the p-type sample showed that impurity scattering is dominant at around room temperature and thus the measured Hall mobility was 0.0065㎡/V-sec at 300K. 7.4±0.6 eV was obtained for acoustic deformation potential in the heavy hole band by fitting the theoretical conductivity and Hall coefficient to the corresponding experimental data. The value is very much higher than the theoretically estimated value, 3.6eV. The carrier density variation with temperature obtained from the present analysis gave 0.38 and 0.17 eV respectively for the activation energies of donor and acceptor.

數學科 問題解決 學習의 指導에 關한 硏究

林在圭,金俊茂,金昭延,李海榮 경북대학교 과학교육연구소 1989 科學敎育硏究誌 Vol.13 No.-

The purpose of this article is to establish some principles in helping students study in order to enhance the ability of solving problems reasonably by using fundamental mathematical skills, and to bring forth the improvement of mathematics education by using the above mentioned principles. In this work we investigate the following subjects ; (1) Fundamental concepts and strategies of teaching students for mathematics problem solving achieved in nineteen eighties in and out of Korea. (2) Current status of problem solving ability of middle and highschool students and teachers. (3) We first made 「motivation-advice cards」 about the problem solving strategy according to the classification of problem solving thought format, and then we used them in highschool classes. We test the result. We obtained the following conclusions ; (1) The style of test problems has to be changed from multiple choice form which is widely used in various evaluation examinations to writing style. (2) Studies of teaching method to help students extend their creativity, and making good problems and invention of instructive devices are required. (3) After making the students aware of the importance of problem solving strategy and of the way how to use it, we have to lead the students to acquire the habit of using them.