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Hydantoin환의 가수분해반응 및 O-알킬화반응을 이용한 새로운 A1ky1 α-Anilinophenylacetate 유도체의 합성
박명숙,최희전,박해선,권순경 德成女子大學校 藥學硏究所 2002 藥學論文誌 Vol.13 No.1
For the development of new synthetic method for unnatural amino acid esters, alkyl α-anilinophenylacetates 4a-i were synthesized through base-catalyzed hydrolysis of 1,5-diphenylhydantoins 1a-b in methanol and O-alkylation of sodium α-anilinophenylacetate 3 with alkyl halides in DMF. Even though hydrolysis of hydantoin ring was undertaken under about 30-40℃ in methanol, the hydantoic acid sodium salt 2 was continuously converted to the sodium α-anilinophenylacetate 3. All synthetic process from hydantoin to alkyl α-anilinopheny lacetates could be carried out in one-pot without isolation of intermediates.
국내에서 유행하는 HIV의 전파 경로에 따른 Subtype 분포
이주실,남정구,김성순,강춘,최병선,김옥진,박미선,성봉모,서순덕,전수경,변승옥,신영오,조해월 대한감염학회 2001 감염 Vol.33 No.5
Background : Previous data have been reported that subtype B is prevalent in South Korea, but neither the extent nor the proportion of subtypes could be evaluated. This study was designed to analyze the distribution of HIV-1 subtypes, temporal instructions and transmission dynamics between epidemiological groups. Methods : 1,280 Koreans had been diagnosed as HIV seropositive during the period 1985 to 2000. Among them, 134 individuals were selected for this molecular epidemiological study. 134 DNAs were isolated from uncultured or cultured peripheral blood mononuclear cells. V3-V5 (0.7 kb) fragment of HIV-1 env gene was amplified by nested polymerase chain reaction and was sequenced. Results : HIV-1 isolates from thirty-seven homosexuals were all subtype B (100%). On the other hand, 66 isolates from 94 heterosexuals were subtype B (70%) and 28 were non B subtypes (30% : 13 A, 4 C, 2 D, 8 E , 1 G). Only subtype B strains were isolated from 73 males who were infected with HIV inside Korea while 16 B and 20 non B subtype strains were isolated from 36 males who were HIV infected outside of Korea. However, B and non B strains were isolated half and half from females who were infected inside Korea except one. Conclusion : The HIV-1 subtype B strains are prevalent in Korea from the early HIV infection until present in both homo and heterosexuals. Non B strains have been transmitted from men who were infected outside Korea to their spouses and casual partners. So, we need further study to monitor subtype B and non B HIV transmission in epidemiological groups of Korea, (Korean J Infect Dis 33:311∼318, 2001)
( Hae Sun Jeon ),( Seung Mi Lee ),( Young Mi Jung ),( Jin Kyun Park ),( Eun Bong Lee ),( Chan-wook Park ),( Jong Kwan Jun ),( Joong Shin Park ) 대한산부인과학회 2019 대한산부인과학회 학술대회 Vol.105 No.-
Objective: Pregnancy in patients with systemic lupus erythematosus (SLE) is associated with an increased risk for maternal and fetal adverse outcomes. Defective placentation plays an important role in the pathogenesis of these adverse outcomes. Also, it has been reported that angiogenic proteins are altered even before the onset of clinical manifestations. In this regard, this study aimed to evaluate the usefulness of the soluble fms-like tyrosine kinase-1 (sFlt-1) to placental growth factor (PlGF) ratio in second trimester plasma of patients with SLE for the prediction of adverse outcomes. Methods: This retrospective cohort study included 30 SLE patients and 9 normal pregnancy controls, who were matched for gestational age at sample collection and for maternal age. The study population was divided into three groups: SLE patients with normal pregnancy outcome (Group 1, n=18), SLE patients with adverse outcomes (Group 2, n=12), and healthy controls (Group 3, n=9). The definition of adverse outcomes was at least one of the followings: intrauterine fetal death, preeclampsia, neonatal death prior to hospital discharge, indicated preterm delivery before 36 weeks, and small for gestational age (<5th percentile) at birth. Second trimester maternal plasma was analyzed for sFlt-1/PlGF ratio using high-sensitive multiplex array. Results: Among 30 women with SLE (Group 1 and 2), adverse outcomes developed in 12 (40%) and the rate of intrauterine fetal death, preeclampsia, neonatal death, indicated preterm delivery, and small for gestational age were 25%, 58%, 0%, 67%, and 54.5%, respectively. The median concentration of the sFlt-1/PlGF ratio was significantly higher in Group 2 compared with Group 1 or Group 3. Receiver operating characteristic analysis revealed the predictability for adverse outcomes based on the sFlt-1/PIGF ratio with AUC of 0.847. Conclusion: Our findings indicate that sFlt-1/PIGF ratio in second trimester maternal plasma can be used to predict the development of adverse outcomes in patients with SLE.
hae sun jang,Hae-Rim Lee,구본상,Eun-Ok Jeon,Moo-Sung Han,Kyung-Cheol Min,Seung-Baek Lee,Yeonji-Bae,Sun-Hyung Cho,Jong-Suk Mo,Jong-Nyeo Kim,모인필 한국가금학회 2014 韓國家禽學會誌 Vol.41 No.3
While use of mass rearing systems improved poultry production, chances of exposing to contagious diseases have been increased, making flocks more vulnerable to diseases. Diseases of interest which affects egg production adversely include Low pathogenic avian influenza (LPAI), Infectious bronchitis (IB), Avian meta-pneumoviral infection (aMPV) and Egg drop syndrome’76 (EDS’76). This report collected and analyzed 5,385 serum samples, which were collected from 1,330 different chicken flock, provided by Chungbuk National University, Avian Disease Laboratory at 2009. Serums were analyzed based on rearing stages; 0∼1.3weeks (wks) (maternal antibody period), >1.3∼3 wks (starting period), >3∼10 wks (growing period), >10∼22 wks (developing period), >22∼40 wks (peak laying period), >40∼60 wks (late laying period) and over 60 wks (post-molting period). Results showed the 99.7% of the tested flocks were immunized against ND and73.8%, 97.1%, 78,2% and 78% of the flocks were immunized against other 4 agents (LPAI, IB, EDS’76, aMPV). Maternal antibody was transferred to enough quantity for NDV. Generally, antibody titers which were developed at 22 weeks were stabilized permanently for life. In case of IB and aMPV, infection titer emerged as early as 10 weeks and the titer was increased from 99.4% to 100% for life. EDS76 showed increase in titers, reflecting decreased frequency of vaccination programs. Overall, this study displayed general trends of major viral disease in layers, but considering the trend of development of preventive measures and evolution of pathogens, conducting serological surveys on a regular basis is important.
서울지역 식육판매업소의 미생물학적 오염도 및 위생관리수준 평가
전해창 ( Hae Chang Jeon ),김지은 ( Jee Eun Kim ),손장원 ( Jang Won Son ),채희선 ( Hee Sun Chae ),진경선 ( Kyung Sun Jin ),오재효 ( Jae Hyo Oh ),신방우 ( Bang Woo Shin ),이정학 ( Jung Hark Lee ) 한국가축위생학회 2011 韓國家畜衛生學會誌 Vol.34 No.4
This study was performed to evaluate the microbial contamination level of meat processing in butcher`s shops in Seoul from January to November in 2010. A total of 695 samples (112 cotton work gloves, 342 utensils and equipments, 241 meat samples) were collected and the environmental hygiene of 89 butcher`s shops was investigated. The aerobic plate count (APC), E. coli and pathogenic bacteria such as Staphylococcus aureus, Salmonella spp. were tested in the samples. As a result, the level of count on APC ranged 0∼2.2×106 CFU/cm2 from the utensils & equipments, 1×102∼2.7×108 CFU/ glove from the cotton work gloves and 1×102∼9.3×106 CFU/g from the meat samples. The level of count on E. coli ranged 0∼2.5×103 CFU/cm2 from the utensils, 0∼8.6×104 CFU/glove from the cotton work gloves and 0∼1.4×105 CFU/g from the meat samples, respectively. Staphylococcus aureus and Salmonella spp. were detected in 11 samples and 2 samples from the cotton work gloves, utensils & equipments, respectively. The sanitation standard operating procedure (SSOP) was applicated at 49 butcher`s shops. In order to improve sanitation of meat in butcher`s shops, in this study, applications of SSOP, systemic sanitation education for employees, hygenic control of utensils & equipments, and continuous monitoring for microorganisms will be required.
Jang, Hae-Sun,Lee, Hae-Rim,Koo, Bon-Sang,Jeon, Eun-Ok,Han, Moo-Sung,Min, Kyung-Cheol,Lee, Seung-Baek,Bae, Yeonji,Cho, Sun-Hyung,Mo, Jong-Suk,Kim, Jong-Nyeo,Mo, In-Pil The Korean Society of Poultry Science 2014 韓國家禽學會誌 Vol.41 No.3
While use of mass rearing systems improved poultry production, chances of exposing to contagious diseases have been increased, making flocks more vulnerable to diseases. Diseases of interest which affects egg production adversely include Low pathogenic avian influenza (LPAI), Infectious bronchitis (IB), Avian meta-pneumoviral infection (aMPV) and Egg drop syndrome'76 (EDS'76). This report collected and analyzed 5,385 serum samples, which were collected from 1,330 different chicken flock, provided by Chungbuk National University, Avian Disease Laboratory at 2009. Serums were analyzed based on rearing stages; 0~1.3weeks (wks) (maternal antibody period), >1.3~3 wks (starting period), >3~10 wks (growing period), >10~22 wks (developing period), >22~40 wks (peak laying period), >40~60 wks (late laying period) and over 60 wks (post-molting period). Results showed the 99.7% of the tested flocks were immunized against ND and73.8%, 97.1%, 78,2% and 78% of the flocks were immunized against other 4 agents (LPAI, IB, EDS'76, aMPV). Maternal antibody was transferred to enough quantity for NDV. Generally, antibody titers which were developed at 22 weeks were stabilized permanently for life. In case of IB and aMPV, infection titer emerged as early as 10 weeks and the titer was increased from 99.4% to 100% for life. EDS76 showed increase in titers, reflecting decreased frequency of vaccination programs. Overall, this study displayed general trends of major viral disease in layers, but considering the trend of development of preventive measures and evolution of pathogens, conducting serological surveys on a regular basis is important.
Jeon, Jun Ho,Park, Deok-Bum,Woo, Sun-Je,Lee, Hae-Ri,Park, Ok-Kyu,Park, Jungchan,Rhie, Gi-eun Elsevier 2018 Cytokine Vol.110 No.-
<P><B>Abstract</B></P> <P>Poly-γ-<SMALL>D</SMALL>-glutamic acid (PGA) of anthrax is an important pathogenic factor due to its anti-phagocytic activity. Additionally, PGA has the ability to activate mouse macrophages for the secretion of cytokines through Toll-like receptor (TLR) 2. Peptidoglycan (PGN), a major bacterial cell-wall component, induces inflammatory responses in the host. We assessed whether PGA can induce maturation and cytokine expression in immature mouse dendritic cells (DCs) in the existence of muramyl dipeptide (MDP), the minimum motif of PGN with immunostimulatory activity. Stimulation of immature DCs with PGA or MDP alone augmented expression of costimulatory molecules and MHC class II proteins, which are all cell surface markers indicative of maturation. The observed effects were further enhanced by costimulation of PGA and MDP. PGA alone was sufficient to induce expression of TNF-α, IL-6, MCP-1, and MIP1-α, whereas MDP alone did not under the same conditions. Treatment with MDP enhanced PGA-induced expression of the tested inflammatory mediators; however, the synergistic effect found for PGA and MDP was not observed in TLR2- or nucleotide-binding oligomerization domain (NOD) 2–knockout DCs. Additionally, MDP augmented PGA-induced MAP kinases and NF-κB activation, which is crucial for expression of cytokines. Furthermore, MAP kinase and NF-κB inhibitors attenuated MDP enhancement of PGA-induced cytokine production. In addition, co-culture of splenocytes and PGA/MDP-matured DCs induced higher expression of IL-2 and IFN-γ compared to that of splenocytes and PGA-matured DCs. Collectively, our results suggest that PGA and MDP cooperatively induce inflammatory responses in mouse DCs through TLR2 and NOD2 via MAP kinase and NF-κB pathways, subsequently leading to lymphocyte activation.</P> <P><B>Highlights</B></P> <P> <UL> <LI> MDP enhances PGA-induced maturation of mouse DCs. </LI> <LI> MDP enhances production of inflammatory mediators by PGA. </LI> <LI> TLR2, NOD2, MAP kinase, and NF-κB signaling pathways are involved in PGA/MDP-induced cytokine production. </LI> <LI> MDP increases lymphocyte activation induced by PGA-matured DCs. </LI> </UL> </P>
Effects of silk fibroin hydrolysate on bone metabolism in ovariectomized rats
( Hae Yong Kweon ),( Sun Hee Shin ),( Jeong Woo Chon ),( Kwang Gill Lee ),( You Young Jo ),( Ji Young Yoon ),( Yoo Kyoung Park ),( Jong Young Jeon ),( Jong Ho Kim ),( Bong Seob Shin ) 한국잠사학회 2015 International Journal of Industrial Entomology Vol.30 No.1
This study aimed to investigate the effects of silk fibroin on bone metabolism in ovariectomized rats. A total of 30 Sprague-Dawley rats were randomized into sham-operated (SHAM), ovariectomized control (OVX), alendronate (OVX+ALEN, 10 mg/kg body weight/d), low silk fibroin (OVX+SF100, 100 mg/kg body weight/d), and high silk fibroin (OVX+SF300, 300 mg/kg body weight/d) groups. All the rats were fed by gavage for 12 wk. At the end of 12 wk, blood and urine were collected for analysis of bone turnover markers, and bone mineral density (BMD) was measured by micro-computed tomography. The results show that the OVX group (p < 0.05) displayed the highest mean body weight gain. Among the five groups, serum levels of bone alkaline phosphatase (ALP) and urine levels of deoxypyridinoline (DPD) were highest in the OVX group (p < 0.05). Bone ALP levels in the ALEN group were significantly lower than that of the silk-treated groups. On the other hand, DPD levels were not significantly different between the ALEN and silk-fibroin-treated groups (p < 0.05). The trabecular BMD was significantly higher in the ALEN and silk-treated groups compared to the OVX group (p < 0.05). In conclusion, this study showed that silk fibroin has similar effects as alendronate, which is used in osteoporosis medication. Therefore silk fibroin might be a new candidate for the prevention and treatment of osteoporosis in patients.
( Hae Min Yang ),( Seong Woo Jeon ),( Jin Tae Jung ),( Dong Wook Lee ),( Chang Yoon Ha ),( Kyung Sik Park ),( Si Hyung Lee ),( Chang Heon Yang ),( Youn Sun Park ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: The Glasgow Blatchford score (GBS) and Rockall scores (RS) are widely used to assess patients with upper gastrointestinal hemorrhage (UGIH). We aimed to compare both scoring systems, and evaluate the clinical usefulness of scoring systems in Korea. Methods: Between February 2011 and December 2013, 1584 patients with nonvariceal UGIH were entered in the study. A prospective study was performed to compare the performance of the GBS, pre-RS, full RS in predicting the need for hospital-based intervention (blood transfusion, endoscopic treatment, angioembolization, or surgery), rebleeding and death. We compared the ability of these scores by using receiver-operating characteristic (ROC) curves. We also assessed the usefulness of GBS of 0 in identifying low-risk patients. Results: For prediction of the need for hospital-based intervention, the GBS was similar to the full RS (AUROC 0.705 vs 0.727; p =0.282) and superior to the pre-RS (AUROC 0.705 vs 0.601; p<0.0001). In predicting death, the full RS was superior to the GBS (AUROC 0.758 vs 0.644; p=0.0006) and similar to the pre-RS (AUROC 0.758 vs 0.754; p=0.869). In predicting rebleeding, the full RS was superior to both GBS (AUROC 0.642 vs 0.585; p=0.031) and pre-RS (AUROC 0.642 vs 0.593; p=0.0003). However none of the systems accurately predicted rebleeding with low AUROC. Of 1584 patients, we identifi ed only 13 patients (0.8%) who scored 0 on the GBS. And one patient with GBS score of 0 required endoscopic treatment. Conclusions: The GBS is more useful than Pre-RS for predicting the need for hospital- based intervention. However cut-off value of 0 for low risk who might be suitable for outpatient management is not useful. The full RS is helpful in predicting death. None of the systems accurately predict rebleeding. (Clinical trial: KCT0000514)