Kidney Toxicity Induced by 13 Weeks Exposure to the Fruiting Body of Paecilomyces sinclairii in Rats

Mihye Jeong,Young-Won Kim,Jeong-Ran Min,Min Kwon,Beom-Suk Han,Jeong-Gyu Kim,Sang-Hee Jeong 한국독성학회 2012 Toxicological Research Vol.28 No.3

Paecilomyces sinclairiis (PS) is known as a functional food or human health supplement. However concerns have been raised about its kidney toxicity. This study was performed to investigate the kidney toxicity of PS by 13 week-oral administration to rats. Blood urea nitrogen (BUN), serum creatinine, and kidney damage biomarkers including beta-2-microglobulin (β2m), glutathione S-transferase alpha (GST-α), kidney injury molecule 1 (KIM-1), tissue inhibitor of matrix metalloproteinase 1 (TIMP-1), vascular endothelial growth factor (VEGF), calbindin, clusterin, cystatin C, neutrophil gelatinase-associated lipocalin (NGAL) and osteopontin were measured during or after the treatment of PS. BUN, creatinine and kidney damage biomarkers in serum were not changed by PS. However, kidney cell karyomegaly and tubular hypertrophy were observed dose-dependently with higher severity in males. KIM-1, TIMP-1 and osteopontin in kidney and urine were increased dose dependently in male or at the highest dose in female rats. Increased urinary osteopontin by PS was not recovered at 2 weeks of post-exposure in both genders. Cystatin C in kidney was decreased at all treatment groups but inversely increased in urine. The changes in kidney damage biomarkers were more remarkable in male than female rats. These data indicate that the PS may provoke renal cell damage and glomerular filtration dysfunction in rats with histopathological lesions and change of kidney damage biomarkers in kidney or urine. Kidney and urinary KIM-1 and cystatin C were the most marked indicators, while kidney weight, BUN and creatinine and kidney damage biomarkers in serum were not influenced.

Kidney Toxicity Induced by 13 Weeks Exposure to the Fruiting Body of Paecilomyces sinclairii in Rats

Jeong, Mi-Hye,Kim, Young-Won,Min, Jeong-Ran,Kwon, Min,Han, Beom-Suk,Kim, Jeong-Gyu,Jeong, Sang-Hee Korean Society of ToxicologyKorea Environmental Mu 2012 Toxicological Research Vol.28 No.3

Paecilomyces sinclairiis (PS) is known as a functional food or human health supplement. However concerns have been raised about its kidney toxicity. This study was performed to investigate the kidney toxicity of PS by 13 week-oral administration to rats. Blood urea nitrogen (BUN), serum creatinine, and kidney damage biomarkers including beta-2-microglobulin (${\beta}2m$), glutathione S-transferase alpha (GST-${\alpha}$), kidney injury molecule 1 (KIM-1), tissue inhibitor of matrix metalloproteinase 1 (TIMP-1), vascular endothelial growth factor (VEGF), calbindin, clusterin, cystatin C, neutrophil gelatinase-associated lipocalin (NGAL) and osteopontin were measured during or after the treatment of PS. BUN, creatinine and kidney damage biomarkers in serum were not changed by PS. However, kidney cell karyomegaly and tubular hypertrophy were observed dose-dependently with higher severity in males. KIM-1, TIMP-1 and osteopontin in kidney and urine were increased dose dependently in male or at the highest dose in female rats. Increased urinary osteopontin by PS was not recovered at 2 weeks of post-exposure in both genders. Cystatin C in kidney was decreased at all treatment groups but inversely increased in urine. The changes in kidney damage biomarkers were more remarkable in male than female rats. These data indicate that the PS may provoke renal cell damage and glomerular filtration dysfunction in rats with histopathological lesions and change of kidney damage biomarkers in kidney or urine. Kidney and urinary KIM-1 and cystatin C were the most marked indicators, while kidney weight, BUN and creatinine and kidney damage biomarkers in serum were not influenced.

역설계 시스템에 의한 자동차용 프레스 제품의 검사

김수용,한민식,김태호,조규재,전언찬 朝鮮大學校 機械技術硏究所 2005 機械技術硏究 Vol.8 No.2

This study is an inspection of press forming product and mould using reverse engineering system. The inspection process on production field involves a lot of errors because of the hand-work so we focussed on improving the measured precision through performing the effective inspection using 3D non-contact scanner. By so doing that. we improved the precision of press forming product by analyzing the cause following the inspection result. Through the inspection, we applied it to the reverse engineering and we could improve the inspection process.

수술을 시행한 치루환자 277예에 대한 분석

박현규,김한선 대한대장항문학회 1996 Annals of Coloproctolgy Vol.12 No.3

물결합재비 및 단위수량에 따른 고유동콘크리트의 제공학적 특성에 관한 실험적 연구

김규용,한장현,신동주,신홍철,김무한 忠南大學校 産業技術硏究所 1998 산업기술연구논문집 Vol.13 No.1

Recently the study on high flowing concrete which has high workability and non-compacting is being proceeded actively in the university and corporative laboratory. There are some cases that has been applied to the field. This high flowing concrete has higher fluidity and segregation resistance than Plain or flowing concrete. And it is being focused as a remarkable know-how which can make high-quality concrete and reduction effect of labor force. This properties of high flowing concrete are influenced by the relationship of several factors; binder content water binder ratio and unit water content. It is the aim of this study to propose reference data at mix design of high flowing concrete, after comparing and analyzing the fluidity and strength properties of high flowing concrete according to water binder content ratio and unlit water content.

CT performance phantom을 이용한 cone beam형 전산화단층영상의 특성

한충완,김규태,최용석,황의환 대한구강악안면방사선학회 2007 Imaging Science in Dentistry Vol.37 No.3

Purpose : To evaluate the characteristics of (widely used) cone beam computed tomography (CBCT) images. Materials and Methods : Images were obtained with CT performance phantoms (The American Association of Physicists in Medicine; AAPM). CT phantom as the destination by using PSR 9000N™ dental CT system (Asahi Roentgen Ind. Co., Ltd., Japan) and i-CAT CBCT (Imaging Science International Inc., USA) that have different kinds of detectors and field of view, and compared these images with the CT number for linear attenuation, contrast resolution, and spatial resolution. Results : CT number of both PSR 9000N™ dental CT system and i-CAT CBCT did not conform to the base value of CT performance phantom. The contrast of i-CAT CBCT is higher than that of PSR 9000N™ dental CT system. Both contrasts were increased according to thickness of cross section. Spatial resolution and shapes of reappearance was possible up to 0.6 mm in PSR 9000N™ dental CT system and up to 1.0 mm in i-CAT CBCT. Low contrast resolution in region of low contrast sensitivity revealed low level at PSR 9000N™ dental CT system and i-CAT CBCT. Conclusion : CBCT images revealed higher spatial resolution, however, contrast resolution in region of low contrast sensitivity was the inferiority of image characteristics.

병재배 폐톱밥을 이용한 양송이 복토재료 개발에 관한 연구

김홍규,이희덕,김용균,한규홍,문창식,김홍기 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-

애느타리 병재배후 탈병한 톱밥을 3월 중순 퇴적하여 부숙시킨 결과 퇴적기간에 따른 폐톱밥의 부숙정도는 퇴적 30일이후에 가장 컸으며 부숙 소요일수는 최소 48일이었다. 퇴적 기간에 따른 폐톱밥의 pH 및 총 질소 함량은 부숙이 진전됨에 따라 증가하는 경향이었고 총 탄소 및 C/N율은 시간이 경과함에 따라 감소하는 경향이었다. 식양토에 부숙이 완료된 톱밥을 부피 비율로 10, 30, 50%씩 첨가하여 복토 재료로 사용한 결과 30% 첨가시 식양토 단용 처리보다 A. bisporus 균사 생장이 촉진되고 초발이 소요일수가 5일 빠르며, 수량에 있어서도 28% 증수되었다. After bottle culture of Pleurotus ostreatus, sawdust was taken out from the bottle and accumulated in the middle of March, and then composted. As the result, Y value was decreased rapidly 30 days after composting, and it was decreased slowly after 30 days. It is considered that 118 days is required for composting, however, it is possible to use for casing material after at least 48 days composting. The pH and total nitrogen content of sawdust based on composting period had tendency to increase as composting was processed. Total carbon and C/N rate had tendency to decrease as time went on. Based on the rate of 10, 30 and 50%, each sawdust was added to clay loam used as casing material for culturing A. bisporrus. Among various treatments, the mycelial growth of A. bisporus was more favorable in the treatment of 30% sawdust than in the single treatment of clay loam. Based on the date necessary for primodium formation of A. bisporus, the primodium formation in the treatment of 30% sawdust was reduced to about 5 days as compared with that of any other treatments When 30% sawdust was added to clay loam used as casing material for culturing A. bisporus, the yield of its fruiting body was increased to 28%.

Chlorpromazine의 유전적 안전성에 관한 연구

김한규,손윤희,이광현,윤태호,남경수 大韓神經精神醫學會 2000 신경정신의학 Vol.39 No.3

연구목적 ; 항 정신성약물로 사용빈도가 높은 chlorpromazine을 사용하여 이 약물의 돌연변이원성과 유전독성을 알아보았다. 방 법 : Bacillus subtilis을 사용한 DNA손상성검토(rec assay), Salmonella typhimurium을 이용한 돌연변이원성실험(Ames test 및 SOS umu test) 그리고 생쥐에 chlorpromazine를 직접 주입하여 대사 후에 돌연변이원성을 확인하는 host-mediated assay법을 각각 사용하였다. 결 과 ; Rec assay에서 chlorpromazine은 Bacillus subtilis의 DNA에는 별다른 영향을 미치지 않았으며, salmonella typhomurium TA 98 및 TA100을 이용한 돌연별이원성 실험에서도 chlorpromazine은 어느 균에도 돌연변이원성을 나타내지 않았으며, S-9 mixture의 첨가에 의해 대사가 된 후에도 돌연변이원성을 나타내지 않았다. 또한 SOS umu test의 경우에도 β-galactosidase활성에는 별다른 영향을 미치지 않는 것으로 보아 chlorpromazine은 돌연별이원성을 일으키지 않는 것으로 판정되었으며 S-9 mixture 처리 후에도 이와 유사한 결과를 얻었다. 한편 생쥐를 이용한 host mediated assay에서도 Ames test에서 S-9 mixture를 첨가 한 경우와 같이 돌연변이원성이 없는 것으로 판정되었다. 결 론 : 이상을 종합해 볼 때 chlorpromazine은 본 실험에 사용한 4가지 방법에서는 돌연병이원성과 유전독성을 일으키지 않는 것으로 나타났다. Object : The aim of this study is to determine whether exposure to chlorpromazine causes mutagenicity and genetic disorders. Method : Ames(Salmonella typhimurium) test and Rec assay(Bacillus subtilis) were used as indicators for DNA damage. Furthermore, the levels of umu operon expression by measuring the β-galactosidase activity were monitered with the SOS umu test using S. typhimurium 1535 containing plasmid pSK1002. And the host-mediated assay was used to investigate the mutagenicity of chlorpromazine after the activation with in vivo metabolic systems. Results : From the results, chlorpromazine did not affect DNA of S. typhimurium and B.subtilis strains and showed no mutagenicity at the all concentrations tested. These phenomena was also similar to that after metabolic activation of chlorpromazine in in vivo system. Conclusion : These results suggested that chlorpromazine did not show the mutagenicity and genotoxicity by four different methods used in this study.

생화학적 표식자를 이용한 골다공증의 평가

이규한,박영재,김미정,이상건,이강목 大韓再活醫學會 1998 Annals of Rehabilitation Medicine Vol.22 No.3

반도체 레이저로 펌핑하는 Nd:YAG 레이저의 내부 발생 제2차 고조파의 On-Off 간헐성에 대한 수치 해석

김규욱,추한태 금오공과대학교 산업기술개발연구원 2000 産業技術開發硏究 Vol.16 No.-

We have made numerical analysis of rate equations for the intracavity second harmonics of ND:YAG laser pumped by laser diode. We can see the very on-off intermittency in the output of the second harmonics when small modulations are applied to laser diode.