Inducing apoptpsis by the inhibition of c-myb in oral squamous carcinoma cell line, KB cell

Lee,Jung-Chang 대한구강생물학회 2007 International Journal of Oral Biology Vol.32 No.4

Oral squamous cell carcinoma (OSCC) is the most commonmalignancy and is a major cause of worldwide cancer mortality.The proto-oncogene c-myb plays an important role in regulationof cell growth and differentiation, and it is expressed at highlevels in hematopoietic cells and many other types of cancers.However, the function of c-myb is not well known in OSCC.The present study aimed to reveal the function of c-myb and totest the alternation of cell growth and signaling by c-myb inOSCC. In this study, c-myb and dominant-negatibe myb(DN-myb) were expressed in an adenovirus-mediated gene deliverysystem to KB cells. The over-expressed c-myb broughtincreased cellular proliferation compared with control cells.However, DN-myb infected KB cells showed significantreduction of cell growth and enhanced induction of apoptosis toactivate PARP and caspase 9. c-myb induced increase of IGF-I,-II and IGF-IR expressions while DN-myb down-regulatedthese expression. Activation of ERK and Akt/PKB pathwaywas shown only in c-myb transduced cells. These findingssuggest that the role of c-myb in cell growth of oral cancer cellsis partially mediated through the modulation of IGFs, ERK andAkt/PKB. From this results, DN-myb is strongly recommendedas a curable gene for the treatment of c-myb dependentmalignancies such as OSCC.

수입 외국산 모발세정용 화장품의 중금속 농도에 관한 연구

문정아,문덕환,박명희,안진홍,김종은,손병철,김대환,이창희,김휘동,이채언 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.5

Objective : For the purpose of preparing the fundamental data and preventing the health impairment due yo heavy metals in hair cleaner. Methods and Material : The author determined the concentration of heavy metals(Fb, fn, Cr, Ni and Cu) in hair cleaner with flameless atomic absorption spectrophotometer. Results : The results were as follows : 1. The mean concentration of heavy metals in total hair cleaner were 0.046㎍/g for Pt, 0.O67㎍/g for Mn, 0.069㎍/g for Cr, 0.046㎍/g for Ni, and 0.022㎍/g for Cu, respectively. 2. There was not statistically significant difference to mean concentrations of each heavy metal in hair cleaner by producing nation. 3. The highest mean concentration of each heavy metal in hair cleaner by type of use were 0.140㎍/g for Mn in sensitive hair cleaner(p<0.05), 0.053㎍/g for Cu in others products(p<0.01). 4. The mean concentration of heavy metals in hair cleaner by type of hair cleaner were high at 0.O54㎍/g for Pb in shampoo products(p<0.O5), 0.120㎍/g for Cr and 0.06㎍/g for Cu in combination products(p<0.01). 5. The mean concentration of heavy metals in hair cleaner by acidity were high at 0.056㎍/g for Pb, 0.124㎍/g for Cr, and 0.032㎍/g for Cu in alkali products (p<0.05). 6. Correlation coefficient of heavy metal concentration in hair cleaner were 0.175 for Pb and Cr, 0.165 for Pb and Mn, 0.824 for Cr, and Mn, 0.189 for Cr, and Ni, 0.406 for Cr, and Cu, 0.197 for Mn and Ni, and 0.375 for Mn and Cu(p<0.05). 7. Exposure amount of heavy metals per when we used lOg of hair cleaner (onetime using amount) were 0.46㎍ for Pb, 0.67㎍ for Mn, 0.69㎍ for Cr, 0.46㎍ for Ni, and 0.22㎍ for Cu, respectively. Conclusion : As above results, there was very low level concentration of heavy metal in hair cleaner but we are using the hair cleaner everyday, therefore we can exposed to heavy metals as cronic exposure so the author suggest to prepare the program of preventing the health impairment due to heavy metals.

월경전증후군의 진단분류학적 개념 및 원인과 치료 : Nosology, Etiology, and Treatment

문석우,서정석,류은정,남범우 건국대학교 의과학연구소 2004 건국의과학학술지 Vol.14 No.-

Since the emergence of the term premenstrual syndrome (PMS) in the 1950s, PMS has become an increasingly discussed topic in popular media sources. Thus self management techniques are easily accessed by women through the media or through their peers. Premenstrual dysphoric disorder (PMDD) comprises extremely distressing emotional and behavioral symptoms. Women with PMDD report premenstrual symptoms, primarily mood symptoms, that are severe enough to seriously interfere with their life style and relationships and usually do not respond to conservative and conventional interventions. Although women with PMS are most often seen in primary care or by their obstetricians/gynecologists, the result of recent study suggested a high rate of missed diagnoses. The premenstrual syndromes include the more common PMS, the less prevalent PMDD, and menstrual psychosis. The etiology of PMS and PMDD is still largely unknown. That PMS and PMDD are primarily biological phenomena is underscored by recent, convincing evidence. Therapeutic interventions for PMS and PMDD range from the conservative to treatment with psychotropic medication and, for the more extreme cases, hormonal therapy or surgical procedures.

무균성 뇌막염에 있어 뇌척수액 추적검사에 대한 재평가

정은경,김경석,김갑승,문경래,박상기 朝鮮大學校 附設 醫學硏究所 1991 The Medical Journal of Chosun University Vol.16 No.1

The author evaluated the clinical manifestation of forty cases of aseptic meningitis who were admitted in the pediatric department of the Chosun University Hospital from March 1990 to September 1990, and also observed the clinical course of two groups of aseptic meningitis divided by admitting time and reevaluated the needness of follow-up lumbar puncture for CSF examination. The author performed follow-up lumbar puncture for CSF examination to the first group, on the 7th hospital day by conventional way, otherwise only observed the clinical course without follow-up lumbar puncture for CSF examination to the 2nd group. There was no difference between both groups, and clinical improvement was seen in both groups. In conclusion, the author considers that if clinical and laboratory finding were compatible with aseptic meningitis, there may be no need for follow-up lumbar puncture for CSF exmenation.

고추 탄저병 저항성 중간모본 ‘원예 100005호 ’,‘원예 100006호’ 육성

양은영,김수,채영,채수영,조명철,문지혜,박태성,이우문,곽정호 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.2

Owing to frequent heavy rain due to abnormal weather, the occurrence of pepper anthracnose has recently increased, which affects the supply and demand of pepper. As a result, the price of red pepper powder in 2011, for example, increased three times compared to the average year. Although anthracnose of pepper plants has been controlled by fungicides until now, it is important to develop new resistant cultivars because of the reduction of the labor force in rural area and occurrence of new anthracnose strains resistant to fungicides. National Institute of Horticultural and Herbal Science developed two pepper lines, ‘Wonye 100005’ and ‘Wonye 100006’ that are resistant to pepper anthracnose. These lines were developed by continuous selection of progenies from a cross between ‘Wonye AR1’, which were developed from interspecific hybridization and ‘TF68’. The anthracnose resistance level of the two lines was higher than that of commercial cultivars and similar to check varieties showing highest level of anthracnose resistance.

전경대원에서 발생한 결핵의 임상 양상

문창기,박상준,조민구,김영중,김소연,김윤권,정준오,안석진,김은실,서승오,김지훈,최원제,이윤영,박형기,최규영,김현근 대한감염학회 2006 감염과 화학요법 Vol.38 No.6

Background: The purpose of this study was to document the incidence and clinical characteristics of patients with tuberculosis (TB) in combat and auxiliary police, living in a group, in Korea where the incidence rate of active TB in a general population is higher than in Western countries. Materials and Methods:We retrospectively reviewed the medical records of all tuberculosis patients diagnosed at National Police Hospital from January 2002 through December 2004. Results:In 2002-2004, a total of 156 cases of tuberculosis were identified with the mean (Standard deviation) age of 20.6 (±1.0) years. Of these, 134 (85.9%) patients were registered as new cases, 11 (7.1%) as relapse, 2 (1.3%) as failure, 5 (3.1%) as treatment-after-default cases whereas 4 (2.6 %) patients were not included in any categories. Average annual new TB rate and smear-positive TB rate were 86.5/10^(5) and 17.4/10^(5) person-years, respectively. In 12 of 31 smear-positive cases, time from onset of symptoms to diagnosis was more than 30 days. Two multidrug-resistance TB cases were identified and two suspected outbreak episodes of TB had occurred during 3 years. Conclusion:There was no statistically significant difference in the incidence rate of new cases of TB between the general population aged 20 to 29 years and combat and auxiliary police in Korea. 배경 : 국내 결핵의 발생은 감소 추세에 있으나 여전히 후진국형 발생양상을 보이고 있다. 특히 집단생활을 하는 젊은이들에서 발생하는 결핵의 유행이 간헐적으로 알려지고 있어 우려를 낳고 있다. 이에 저자들은 최근 전경대원의 결핵 발병 양상에 대해 알아보고자 본 연구를 시행하였다. 재료 및 방법 : 2002년 1월부터 2004년 12월까지 3년간 경찰병원에서 결핵 진료를 받은 전경대원의 의무기록을 후향적으로 분석하여 연도별 결핵 신환발생률, 도말양성결핵 신환율, 진단 지연, 집단발병, 다제내성결핵을 조사하였다. 결과 : 연도별 결핵 신환자 수(10만명당 발생추정치)는 2002년 50명(83.6명), 2003년 42명(83.7명), 2004년 42명(93.5명)이었고 도말양성 폐결핵 신환자 수는 2002년 10명(16.7명), 2003년 11명(21.9명), 2004년 6명(13.4명)이었다. 3년간 도말양성 폐결핵 환자는 31명이 있었고 그 중 12명은 호흡기 증상 발생 30일이 지나서 진단을 받았다. 다제내성 결핵은 2명이 있었으며 결핵의 집단발병이 의심되는 사례는 2차례 있었다. 결론 : 전경대원과 20-29세 연령군 일반인의 결핵 신환발생률, 도말양성 폐결핵 발생률의 통계적으로 유의한 차이는 없었다.

양호교사의 역할수행에 관한 조사연구

문희자,박신애,강현숙,고정은 慶熙大學校 1985 論文集 Vol.14 No.-

It is the ultimate purpose of school health management to protect, maintain, and prormote the students' health and to help for them to form a wholesome habit of health. To do this, school nurse is an actual professional personnel of school health management in our country. therefore, role performance of a school nurse is directly related to the quality of school health planning, we think. With this belief, this study intends to revise, supplement Rustia model for school health promotion and develop in into a suitable model for our actual situation. In so doing, based on Rustia model, we had surveyed the actual situation of a school nurse's role performance by making 77 school nurses in Boys' and Girls' Middle and High Schools in Seoul as the scope of this research for a period of two months beginning on September 1, 1984. The results were analyzed by employing percentage and mean value. The results of the investigation are as follows: 1. General Characteristics of the school nurses and schools. (1) The average age of the surveyed school nurses is 31.64± 6.6 years old. 81.6% of them was proved to have acquired academic background above Colleges and Graduate Schools; 74% were married; 61% were religious persons. And the average on-duty term was 6.42±5.03 years. 66.2% of them employed in the hospitals before this present job and 31.2% have worked at school as school nurses immediately after their graduation. In college, most of them (81.8%) went through a course of study for the teaching profession and only 9.1% of the taught other courses of study. (2) The majority of the surveyed schools (51.9%) were public schools and 62.3% of them were middle Schools. The average number of students in the school was 2628.52;that of school pesonnel was 66.17±9.7;that of classes was 39.68±12.0. 2. Role performance for primary prevention. (1) Among the serveyed schools, 83.1% of them performed epidemiological analysis of health problem for the promotion of health. As for health planning among health management plan, all of them put it in force, however, only 35% of them participated in preparation of the budget to achieve it. As to the evaluation program, 64.9% of them carry out it into operation and in case of being carried out once in a year marked the most frequency of enforcement as 32.4%. On guidance and support related to health planning, 42.9% of them held a staff meeting; of which 53.2% were school nurse-teacher meeting; 28.6%, school nurse-parent meeting. (2) To protect the students and school personnel from danger and injury, 67.5% of them participated in alleviating health problems-that is, the maintenance of health standard and safety within school, and inserting the means of settling those problems into curriculum and management of school events. Especially, only 14.3% administered school lighting and 35.1% observed safety standards of desks and chairs in class. Consequently, the result of this survey revealed that there is much to be desired in the administration for the maintenance of health standard and safety. As for pervention of infectious disease, the following were implemented; a) disinfection of toilets (92.2%); b) control of drinking water (75.5%); c) purification of sewage and garbage incineration (74%). What is pecular is that 71.4% of them have controlled vaccination but enforcement rate shows lack of enthusiasm when viewed from the kinds of vaccination: Typhoid fever (51.9%), cholera (41.6%) hepatitis (9.0%), encephalitis (23.4%) and that one or two sorts of vaccination were treated. As for the technical improvement of personnels who handled health problems, all of them put it in practice, and for prevention program and use of health resources, 87.5% participated in it. 3. Role performance for secondary prevention (1) 92.2% of them carried out screening tests of a physical assessment for an early detect on health problem and 24.7% did a screening tests which made a part of students its object after the first screening tests. A selection and check-up of measurement tool during a developmental evaluation was taken effect by 98.7% and a preliminary education on the persons who administered that examination and determine to select a place for it by 100%. Of discrimination and screening tests, various kinds of examination for finding disease was detected effectively by 98.7% and of this operation nothing could be come into force more than a check-up of parasites and urine with 97.4%. As to the methods explaning danger of health and safety, the case to be offered school personnels information in a staff meeting marked 96.1% and the one to offer students information in the case of generating such danger as infectious diseases was 100% (2) Control of health problem to be required regular treatment for taking a proper measure on that problem at an earlier time was carried into effect by 93.5%. 98.7% of them imposed a role of communication and intervention of offering and observation method for finding health problem and encourging this method to teacher in charge. All of them executed emergency care, School-nurses helped 33 persons a day on the average, and 89.6% of them did for students to get through a serious crisis. (3) In order to prevent complication and an disable, 83.1% of them carried out a continuous follow-up care of the students who were discharged from the hospitals. 4. Role performance for tertiary prevention (1) In order to care and treat the students who needed their rehabilitation and adaptation for activities of daily living, 89.6% of them performed reeducation after reevaluate their health status, 94.8% performed a role on motivation, 85.7% put in effect a continuous control of the students who needed rehabilitation and correction, and 15.6% did counselling for taking a measure to meet the situation of the disabled. 5. Role performance for health education. Unifying health education from the view points of the first, secondary and tertiary prevention against diseases, we surveyed the content, method, objectives of health education which is now in effect and should be emphasized in the future. Upon this study, we came to conclude as the following; as to the content of health education which is now is effect, prevention of infectious diseases has an absolute majority of all contents as 98.7%, and prevention against parasites, personal hygiene and oral hygiene second place. as 97.4% But education on prevention of venereal diseases shows inactiveness as 42.9%. On the contents to be emphasized, mental health (42.9%), sex education (40.3%) were enumerated but nobody stated about prevention of infectious diseases. As a method practising health education, all of them used that of counselling, and as an indirect education utilizing media, it comes printed matters (96.1%), poster (83.1%), interclass broadcasting casting (75.35) in that order. And the case of regular class on health education marked 23.4%, of the object of health education, 98.7% made students its object; 87.0% school personnels; 51.9% parents of students.

소아 대퇴 근위부 골절의 치료결과

정성택,이근배,윤택림,문은선,송은규 全南大醫大 2001 전남의대학술지 Vol.37 No.1

저출산･고령사회 이슈에 대한 정책대응 과제 : 인천시 사례를 중심으로

문은영,권미애,전혜정 이화여자대학교 젠더법학연구소 2016 이화젠더법학 Vol.8 No.1

This study was conducted with the need for preparing active measures of the central government and the local government to deal with low fertility and aging society issues and with the purpose of setting the related policy direction. The issues of recent low fertility and aging are experienced globally and the decline of population is detected in Korea as well. Therefore, multilateral efforts are taken to deal with the imminent phenomenon at the governmental level. Incheon Metropolitan City, with its population of 3 million, is one of the big cities in rapid progress of low fertility and aging phenomenon. While the total fertility rate of Incheon is 1.21 (Statistics Korea, 2015) which is the similar level with the national average, it is far below the population replacement level and has continuously been recording the low figures since 2000. Besides, the elderly population rate in 2014 was 10.3%, a 5.5% increase compared to the previous year. It is expected that the population aging would be accelerated for the next 30 years and it is forecasted that the elderly population rate of Incheon would reach around 30% by 2040. According to the survey results of general citizens on this population change, more than half of the respondents seriously recognized the shift to the low fertility and aging society and demanded the measures to deal with the issues. To set the policy direction in association with the central government within the local region, this study explores the tasks to deal with the policy and the demands for the policy in Incheon Metropolitan City. 본 연구는 저출산・고령사회 이슈에 대응하여 중앙정부와 더불어 지자체의 적극적 대책마련의 필요성과 정책방향성 수립을 위한 목적으로 시도되었다. 최근 저출산과 고령화의 문제는 전 세계적으로 경험되는 문제이며 또한 우리나라에서도 인구의 변화가 감지되고 있는 상황이다. 이에 정부차원에서 이미 저출산과 고령화 문제를 경험하고 대처하고 있는 선진국의 사례를 검토하면서, 다가올 현상에 대해 대응할 수 있기 위한 다각적 노력이 진행되고 있다. 인천시는 인구 300만 시대가 임박하는 상황에서 저출산과 고령화 현상이 빠르게 진행되고 있는 대도시중 하나이다. 인천시의 합계출산율은 1.21명으로 전국평균과 동일한 수준에 있지만 인구대체수준에는 크게 못 미치는 상황이며, 2000년 이래 지속적으로 낮은 수치를 기록하고 있다. 또한 2014년 노인인구 구성비는 10.3%로 전년대비 5.5% 증가되었으며, 향후 30년간 고령화가 빠른 속도로 진행될 것으로 전망되며 오는 2040년 인천의 고령인구비율이 약 30%로 예측되고 있다. 이러한 인구변화에 대해 일반 시민들에게 저출산・고령화 사회로의 변화는 실태조사결과 절반 이상이 심각하게 인지하고 있는 상황이며, 이에 대한 대책마련을 요구하고 있는 것으로 나타났다. 본 연구에서는 중앙정부와 연계성 있는 지역 내 정책방향 수립을 위해 인천시 사례를 들어 지역에서의 정책에 대한 시민체감도와 정책 수요, 또 정책대응의 과제를 살펴보고자 한다.

소아 백혈병의 혈청 철, 총 철결합능 Transferrin포화율 및 Ferritin치에 관한 연구

문경래,정은경,박상기 朝鮮大學校 附設 醫學硏究所 1991 The Medical Journal of Chosun University Vol.16 No.1

Author reviewed the clinical symtoms, hematological findings and serum Fe, total iron binding capadty(TIBC), transferrin saturation and levels of 40 children diagnosed as leukemia at the pediatric department of Chosun University hospital during a period of 7 years from Jan. 1983 to Dec. 1989. Also, 20 normal children as control group were studied. The results were summarized as follows: 1) The Mean±S.D. levels of the serum Fe, TIBC, transferrin saturation and ferrtin of leukemia 40 cases and control group were 126.8±37.8㎍/㎗, 276.1±37.8㎍/㎗, 47.1±15.6%, 244.5±52.7ng/㎖ and 107.2±34.2㎍/㎗, 309.9±31.8㎍/㎗, 34.7±11.4%, 69.6±30.8ng/㎖, respectively. The difference of the serum Fe levels between leukemia and normal control was statistically not significant (P>0.1) but the serum Fe, TIBC, transferrrin saturation and ferritin levels were significantly different(P<0.05, P<0.01, P<0.001). 2) The differences in the mean levels of the serum Fe, TIBC, transferrin saturation and ferfitin among the types of leukemia were not significant. 3) The differences of the serum TIBC, transferrin saturation and ferritin levels between leukemia and normal control were statistically significant. From the very high serum ferritin levels, it is thought that serum ferritinemia as tumor marker may be useful in diagnosis of leukemia.