The expression patterns of RANKL and OPG in murine tooth eruption

Hwang, Kyung-Mun,Kim, Eun-Jung,Kim, Hyun-Jung,,Kim, Young-Jin,Nam, Soon-Hyeun 大韓小兒齒科學會 2006 大韓小兒齒科學會誌 Vol.33 No.2

치아의 맹출은 치아기 (dental organ)와 치조골의 세포와 연관된 매우 복잡한 과정이다. 우선 치아 맹출이 일어나기 전에 파골세포가 치낭으로 집결하게 된다. 이러한 치낭의 역할은 파골세포와 조골세포의 상호작용으로 이루어 지는 골개조와 밀접한 관련이 있는데, 이는 치아 맹출과 연관된 많은 유전자들이 치낭에서 발현되기 때문이다. RANKL는 TNF ligand family로써 조골세포에 존재하며 파골세포의 형성 및 전구세포로 부터의 활성화를 유도한다. 이러한 RANKL은 OPG에 의해 그 작용이 억제되며 RANKL와 OPG의 상대적인 비율이 파골세포의 형성에 영향을 미친다. 또한 Runx2유전자의 변이는 조골세포의 분화와 활성에 차질을 가져오고 결국 RANKL/OPG pathway를 통해 파골세포 형성에 영향을 줄 수 있다. 치아의 발육 및 맹출에 미치는 RANKL 및 OPG의 영향을 알아보고 Runx2와의 연관성을 알아보기 위해 in situ hybridization 방법으로 태생 1, 3, 5, 7, 9, 11일된 쥐의 하악 및 제1대구치를 사용하여 실험을 실시한 결과 RANKL, OPG, Runx2의 mRNA가 태생 1일부터 11일까지 치낭 및 치아주위조직에 특성있게 나타났다. 이중 태생 5일에서 9일 사이에 RANKL 및 Runx2는 치아의 교합면측과 하방 치조골 부위의 발현이 강하게 나타난 반면 OPG는 약한 발현을 보였다. 이는 또한 파골세포의 활성부위를 알아보기 위해 TRAP염색을 실시하여 태생 5일에서 9일 사이에 최대의 활성화를 나타낸 결과와 연광성 있게 나타났다. RANKL, OPG, Runx2의 특성있는 발현양상들을 종합해 볼 때, 치아 맹출은 치낭, 치아기, 치조골 사이의 상호 작용을 통해 이루어 지며, 이는 치낭이 치아 맹출에 있어서 매우 중요하다는 것을 의미한다. 또한, 이러한 유전자들 (RANKL, OPG, Runx2) 이 치아의 맹출에 중요한 역할을 하는 것으로 사료된다. Tooth eruption is a complex and tightly regulated process that involves cells of the tooth organ and the surrounding alveolus. Osteoclast precursors must be recruited into the dental follicle prior to the onset of eruption. This function of dental follicle may be regarded as the ability of bone remodeling characterized by the interaction of osteoclasts and osteoblasts. This is because tooth eruption is a localized event in which many of the genes required for eruption are expressed in the dental follicle. RANKL is a membrane-bound protein that is a member of the TNF ligand family, which is present on bone marrow stromal cells and osteoblasts, and induces osteoclast formation and activation from precursor cell. The biologic effect of RANKL is inhibited by OPG and, in bone, the relative ratio of RANKL and OPG modulates osteoclastogenesis. To evaluate the roles of RANKL and OPG in tooth eruption and the relations with the expression pattern of Runx2, in situ hybridization was performed with mandibles of mice at postnatal stage 1, 3, 5, 7, 9 and 11. mRNA of RANKL, OPG, and Runx2 are expressed in dental follicle and surrounding tissue from P1 to 11. To determine the sites of osteoclastic activity duing tooth eruption, mandibles were dissected. Peak osteoclastic activity in alveolar bone along the occlusal and basal regions was observed from P5 to 9, with osteoclasts in these regions being large and strongly TRAP-positive. The specific spatic-temporal expression patterns of RANKL, OPG and Runx2 in our study suggest that tooth eruption could be progressed through the interactions of molecular signaling among dental follicle, dental organ and alveolar bone, furthermore it means that dental follicle is quite important in tooth eruption. In addition, it indicates that these genes (RANKL, OPG, and Runx2) play critical roles in tooth eruption.

급성 골수성 백혈병 환자에서 관해 유도 항암 화학요법 후 발생한 베르니케 뇌증

황진원,정재현,서광원,최병진,송영진,김종윤,이은지,임홍규,이상민 인제대학교 2009 仁濟醫學 Vol.30 No.-

Wernicke's encephalopathy is characterized by the triad of ocular symptoms, ataxia, and mental confusion and neuropsychiatric condition generally caused by acute thiamine deficiency. Although it is common in the severe alcoholics, several other causes also have been identified, such as total parenteral nutrition (TPN) use, hyperemesis gravidarum, anorexia nervosa, hemodialysis, uremia, and acquired immunodeficiency syndrome(AIDS). Chemotherapy in patient with malignanacy and long term total parenteral nutrition following bone marrow transplantation are related with Wernicke's encephalopathy. A 48-year-old woman had been treated with Daunorubicin and Cytarabine for remission induction chemotherapy of acute myeloblastic leukemia and intravenous hyperalimentation due to persistent hematochezia and ileus caused by infiltration of leukemic cell in terminal ileum. She suddenly complained of diplopia, tremor, and mental confusion at 32th day after chemotherapy and generalized tonic-clonic seizure at 34th day after chemotherapy. The diagnosis of Wernicke's encephalopathy was made with clinical presentations and brain MRI. Brain MR T2-weighted & Flair image revealed a high signal intensity lesions bilaterally at the medial thalamus and peri-acueductal grey matter of the midbrain. The patient was successfully treated with vitamin B1. We presented a case of acute Wernicke's encephalopathy developed after remission induction chemotherapy followed by intravenous hyperalirnentation in a patient with acute myeloblastic leukemia.

DIS80의 遺傳子型 決定을 위한 PCR法의 條件에 관한 硏究

鄭宰安,黃迪駿,李羲碩,宋垠燮 大韓法醫學會 1994 대한법의학회지 Vol.18 No.1

The polymerase chain reaction(PCR) is known to be a very sensitive method of nucleic acid synthesis by which a particular segment of deoxyribonucleic acid(DNA) can be specifically amplified. However, as no single protocol will be appropriate to all situations, it requires to optimize the PCR conditions for a given application, especially analytical procedure to amplified fragment length polymorphism of VNTR region. Recently, typing of VNTR regions in the field of forensic science is almost done by PCR, but this reaction ofter results in a number of potential problems including generation of recombinant alleles during the extension phase. The objective of this paper is to evaluate the cause of PCR products of a abnormal length, and to set up the guideline for the reliable production of D1S80VNTR region in the human genome by studying several parameters that influence polymerase chain reaction. In the study of D1S80 VNTR region to be amplified, the following PCR conditions are optimal in the 50㎕ of reaction volume and under the temperature conditions of 95℃ for 1 minute, 65℃ for 2 minutes and 72℃ for 2 minutes. 1. The adequate concentration of MgCl₂ is within the range of 1.0 to 2.0 mM. 2. The high activities of Taq polymerase show about 0.5 to 1.5 units without generation of the undesired products. 3. The adequate amount of template DNA is about 30 to 50ng, although the template DNA can be amplified with the minimum of 40pg. 4. With the 50ng of template DNA, the adequate numbers of PCR cycle range 25 to 27 cycles. 5. Artifact production by PCR is minimal within the range of 0.2 to 0.3μM of each primer concentration. With the above conditions, amplified fragment length polymorphism (AMP FLP) obtained by polymerase chain reaction from the genomic DNAs of 4 Korean families (one family with 8 offsprings and both parents, 3 families composed of each one child and both parents) were analyzed to test the applicability of this technique to paternity testing. The results obtained present reliable parent child relationships based on the correct genotypes of D1S80, without any undesired PCR products of abnormal length that are postulated as heteroduplex resulting from cross annealing or hybridization between the amplified target DNA.

운동프로그램이 뇌성마비인의 자아존중감 (Self-Esteem)에 미치는 영향

황명자,함정은 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.14 No.3

The purpose of this study is to examine the influence of exercising program on self-esteem for people with cerebral palsy. The exercising program was the Hwal In Shim Bang exercising program of Lee Hwang with the pen name of Twaegae. The Hwal In Shim Bang exercising program was applied to the experiment group for 50 minutes 3times a week for a total of 12 weeks and the subject of this study was 16 male and female high school students in a special school from 17 to 25 years of age(8 students in the experiment group and 8 students in the control group). In order to verify the effectiveness of exercising program, the self-esteem inventory were conducted before and after exercising program. The results were summerized as follows; 1. The self-esteem before and after applying the Hwal In Shim Bang exercising program, there was significant difference in the general self-esteem field (t=7.20, p<.001), study accomplishment field (t=4.97, p<.01), social acceptance field (t=2.76, p<.05), physical expression field (t=3.33, p<.05), characteristics of actions (t=4.26, p<.01), motor fitness field (t=3.55, p<.01) and the whole of self-esteem (t=11.50, p<.001) in the experiment group. 2. The self-esteem before and after applying the Hwal In Shim Bang exercising program, there was no significant difference in all six fields of self-esteem and self-esteem on the whole in the control group. With the above results, it can be concluded that the Hwal In Shim Bang exercising program is a effective exercising program for increasing the self-esteem of people with cerebral palsy with the physical and psychological defect.

선천성 완전방실차단이 동반된 신생아 루프스 1예

정재현,이은지,이은주,황진원,송영진,최병진,김일환,김종윤,허민영,김동욱 인제대학교 2009 仁濟醫學 Vol.30 No.-

Neonatal lupus is a rare syndrome occurring In neonates with tranplacentally acquired maternal autoantibodies, In particular anti-SSA/Ro-SSB/Ra antibodies. Manifestations include congenital heart block, cutaneous lesions, thrombocytopenia, neutropenia, pulmonary or neurological disease and hepatitis. The non-cardiac manifestations resolve by 6 months of age, congenital heart block is irreversible. We experienced a case of neonatal lupus with congenital complete heart block in a newborn of asyptomatic mother with anti-SSA/Ro-SSB/Ra antibodies.

고립성 폐 결절로 나타난 경화성 혈관종의 폐엽 절제술에 의한 치험 1예 : A case report

황은구,이송암,황재준,김상윤,권혁중,김요한 건국대학교 의과학연구소 2004 건국의과학학술지 Vol.13 No.-

Patient was sixty-eight years old female. She was transferred to our department to resect the solitary pulmonary nodule on right lower lobe. Intraoperative frozen-section revealed bronchioloalveolar carcinoma. So, we performed right lower lobectomy and mediastinal lymph node dissection. Permanent pathologic diagnosis of this patient was sclerosing hemangioma. We report experience of treatment of sclerosing hemagioma presenting solitary pulmonary nodule by lobectomy with the review of literatures.

만성정신질환자의 직업재활 사례

황태연,한은선,이영문,강순정,정경선 용인정신의학연구소 1995 용인정신의학보 Vol.2 No.1

축구선수의 Detraining과 Retraining이 혈청지질 및 호르몬농도에 미치는 영향

정정화,박재현,채종훈,성혜련,황지인,윤미숙,노금선,윤종관,윤영학,노순덕,정경숙,박일규,김은희,박현태,박상갑 대한스포츠의학회 1999 대한스포츠의학회지 Vol.17 No.1

The purpose of this study was to investigate the effects of detraining and retraining on serum lipid and hormones in soccer players. Subjects were seven male high-school soccer players. V˙O_2max was determined for each subjects by administering a treadmill test(initial speed: 90m/min, grade: 5%, increasing speed per 3 min: 30m/min). Serum lipid(T-C, TG, HDL-C LDL-C) and hormones(epinephrine, norepinephrine, growth hormones, cortisol) were assayed pre and post detraining in 10, 20, 30 days after retraining. The repeated ANOVA was used to determine significant differences. The 0.05 level of significance was as critical level for the study. The results of the study were as follows: 1. V˙O_2max(ml/min) were 3576.3±204.2ml/min pre detraining, 3234.1±198.9 ml/min post detraining. There are significant(p<.05) difference between pre and post detraining. In 10, 20, 30 days after retraining, V˙O_2max(ml/min) were 3601.4±170.9 ml/min. There were significantly(p<.05) increased in retraining periods. 2. V˙O_2max(ml/kg/min) were significantly(p<.05) decreased from 62.3±2.9 ml/kg/min to 55.9±4.7 ml/kg/min in detraining. In 10, 20, 30 days after retraining, V˙O_2max(ml/kg/min) were 62.4±3.4ml/kg/min, 62.7±2.3ml.kg/min, 67.3±7.2ml/kg/min respectively. There were significantly(p<.05) increased in retraining periods. 3. T-C were significantly (p<.05) increased from 166.6±8.5mg/dl to 175.3±10.3 mg/dl in detraining. In 10, 20, 30 days after retraining, T-C were 160.1± 3.2mg/dl, 156.7±3.7mg/dl, 140.3±9.0mg/dl. There were significantly(p<.05) decreased in retraining periods. 4. HDL-C were 61.4±6.6mg/di pre detraining, 5.3±6.6mg/dl post detraining. There are significant(p<.05) difference between pre and post detraining. In 10, 20, 30 days after retraining, HDL-C were 56.9±7.1mg/dl, 56.4±9.2mg/dl, 57.7±9.1mg/dl respectively. There were no significant difference in retraining periods. 5. The hormones(epinephrine. norepinephrine, growth hormone, cortisol) were changed as same patterns. Epinephrine were 26.0±7.0[g/ml pre detraining, 24.6±3.2pg/ml post detraining. In 10, 20, 30 days after retraining, epinephrine were 26.9±5.6pg/ml, 30.6±6.2pg/ml, 29.4±5.6pg/ml respectively. There were no significant difference in retraining periods. In conclusion, HDL-C, epinephrine, norepinephrine, growth hormone and cortisol were decreased, T-C, LDL-C and TG were increased in detraining. But HDL-C, epinephrine, norepinephrine, growth hormone and cortisol were increased, T-C, LDL-C and TG were decreased in retraining.

쌍발 복합재 비행기의 속도, 고도 보정 및 상승성능에 관한 연구

황명신,박윤진,이정모,김칠영,은희봉 한국항공운항학회 1997 한국항공운항학회지 Vol.5 No.1

Airspeed and altimeter calibration of "win Mee was conducted by the flight test. We have adopted system to system method. Flight test data is corrected for instrumented error and position error, and the resultin data was satisfied. Climb Performance flight test also was conducted. But we could not have all data because of limited flight time. The resulted data was satisfied compare with calculated dab.

주부들의 의복 관여도와 유아복 잡지광고에 대한 태도 고찰

황춘섭,오은정 慶熙大學校 1996 論文集 Vol.25 No.-

This research is interested in the concept of consumer's fashion involvement which makes influences on the consumer's information-searching pattern. The main object of the approach is the mothers of the babies under the age 3, who can be considered the actual babies' costumes buyers. The writer tried to find out whether the mother's own fashion involvement is in any proportionate relation with their babies' costumes involvement and also to study their various attitudes toward the magazine advertising of babies' costumes. The writer hope this research could provide babies' costumes industries with basic data that can help to establish more effectual advertising strategies. To achieve the purpose of the present research. The writer established 4 more detailed study points as follows: 1) to find out the appeal patterns of babies' costumes advertisements especially favored by the mothers 2) to find out the relationship between the mothers' own fashion involvement and their involvement in the babies'costumes advertisement 3) to find out their various degree of involvement in the babies' costumes advertisement according to their fashion involvement degree. This research was conducted through the survey method using stimulating objects and questionnaires. The experimental advertisements used in this survey were selected among the actual magazine advertising, and reproduced with the help of an expert in advertisement production who is a present member of an advertising company. During the reproduction process, all the brand names were deleted in order to control the brand familiarity and the brand preference which might have an influence on the consumer's attitude toward specific advertisements. The appeal patterns of the magazine advertising materials used in this research was limited to only 3 kinds that is, the appeal to reason, the mixed appeal to reason and emotion, the appeal to emotion. The questionnaire includes question items designed to identify the attitudes toward advertisements, the degree of fashion involvement, the opinions on utility of magazine advertising of babies costumes, and the present magazine subscription situation, the source of information on babies' costumes purchase, 394 samples were extracted through the convenience sampling method among the mothers of babies under the age 3. presently living in Seoul. The data was analyzed by Frequency, paired t-test, Chi-square, Correlation and Oneway. The result of the research can be summarized as follows: 1) Among the 3 kinds of advertisement appeal patterns, most preference was shown to the mixed appeal to reason and emotion. The appeal to emotion followed next and then comes the appeal to reason. 2) The higher the degree of mothers' own fashion involvement, the higher their involvement in their babies'costumes. 3) Mothers' fashion involvement degree doesn't make any difference in their attitudes toward the advertisement of babies' costumes.