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Dong-il “Dan” Cho 제어로봇시스템학회 2017 제어로봇시스템학회 국제학술대회 논문집 Vol.2017 No.10

알칼리환원수 음용이 급성 염증성장질환 생쥐 모델에 미치는 영향

김단,김동희,등영건,최주봉,이규재 韓國電子顯微鏡學會 2008 Applied microscopy Vol.38 No.2

알칼리환원수 (Alkaline Reduced Water: ARW)는 아시아를 비롯한 여러 나라에서 음용수로 사용되고 있으며 항산화효과와 장내이상발효 개선효과 등을 중심으로 안전성과 유효성의 검증이 이루어지고 있다. 본 연구에서는 알칼리환원수가 급성 염증성대장질환 (Inflammatory Bowel Disease: IBD) 동물모델에 미치는 영향을 알아보기 위해, ARW를 2주 동안 급이 시킨 후 4% DDS로 염증성 대장염을 유발시키고 대장의 길이와 조직병리학적 변화를 관찰하였다. 그 결과 대장의 길이와 육안적, 현미경적 질환점수의 수치가 대조군과 비교하여 유의한 차이를 나타내지 않았다. 이 결과는 DSS로 유도된 급성 염증성장질환 모델에서는 2주 동안의 ARW 급이가 개선효과를 나타내지 않았음을 말해준다. 그러나 ARW 가 장내환경을 개선시키고 위장관의 질환을 개선시키는 효과가 있음을 고려해볼 때 급성 IBD 동물모델이 ARW의 효과를 증명하기에 적합하지 않았거나 MARW의 급이기간이 장내환경을 개선할 만큼 충분히 길지 않았을 가능성을 추측해 볼 수 있다. 알칼리환원수의 장내이상발효 개선효과는 확인되고 있으나 그 기전은 아직까지 구체적으로 확인되어지지 않고 있다. 이번 연구결과에 의하면 인위적으로 유발시킨 급성 염증성 동물모델에서 ARW가 유의한 영향을 미치지 않았지만, 다른 장질환 모델을 이용한 효과 검증과 ARW의 장기 급이에 따른 효과, 그리고 ARW 가 장내환경에 미치는 작용기전에 대한 연구가 더 깊이 있게 이루어져야 할 것으로 사료된다. Alkaline reduced water (ARW) has been used for drinking widely in several countries of Asia. The safety and clinical effects of ARW has been reported including anti-oxidative effect and intestinal abnormal fermentation. To confirm the effect of ARW on DSS-induced acute inflammatory bowel disease (IBD) mouse model, we observed the length of total large intestine and the histopathological changes after supplying mineral induced-ARW (MARW) for 2 weeks and oral administration of 4% DSS (dextran sulfate sodium). As the result, the length of total large intestine and the disease scores by macro and microscopical access in the ARWsupplied group showed no significant differences compared with those in the control group. This result suggests that the supply of ARW for 2 weeks exerted no effect on amelioration in the DSS-induced acute IBD model. However, in consideration of the effect of ARW on the improvement of intestinal environment and gastrointestinal disease, this result seems that acute IBD animal model is not suitable or the period of ARW supply is not enough to prove the effect of ARW. The ameliorative effect of ARW on the intestinal abnormal fermentation has been confirmed by some researchers, but the precise mechanism also remain unclear. In conclusion, although MARW had no effect on the DSS-induced acute experimental colitis model, further studies on the verification of the effects of ARW by using other intestinal disease model and by long-term supply of ARW will be required. Also, It needs to clear the mechanism of ARW on the intestinal environment.

Berberine의 항생제 내성 확색포도구균에 대한 항미생물 효과

김강주,차정단,김해경,유현희,유용욱,이동근 대한악안면성형재건외과학회 2002 Maxillofacial Plastic Reconstructive Surgery Vol.24 No.2

Antibiotic-resistant Staphylococcus aureus (S. aureus) emerged in many parts of the world and increased complex clinical problems. Therefore, new agents are needed to treat the antibiotic-resistant S. aureus. Some natural products are candidates of new antibiotic substances. Previous reports showed that the extract of Coptidis Rhizoma and Phellodendri Cortex have antimicrobial effects. The main antibacterial substance of Coptidis Rhizoma and Phellodendri Cortex is berberine. In this study, we examined the antimicrobial activity of berberine to antibiotic-resistant S. aureus. We isolated the 13 strains of antibiotic-resistant S. aureus from the oral cavity of healthy children in a preschool, and investigated the antimicrobial activity of berberine. We examined the effects of berberine on the level of resistance to antibiotic-resistant S. aureus. We observed that the invasion of S. aureus into human gingival fibroblasts was blocked by berberine. Berberine inhibited the growth of antibiotic-resistant S. aureus as a dosw dependent maner. Minimum inhibitory concentration (MIC) of berberine against antibiotic-resistant S. aureus was 50∼100㎍/㎖. The inhibition of S. aureus invasion was depended on the concentration of berberine in human gingival fibroblasts. The MIC of ampicillin against antibiotic-resistant S. aureus was 32㎍/㎖. However, the MIC of ampicillin against antibiotic-resistant S. aureus in the presence of 50㎍/㎖ berberine was 1㎍/㎖. These data indicate that the combination with berberine and β-lactam antibiotics resulted in the synergistic reaction. These results suggest that the berberine may have the antimicrobial activities to antibiotic-resistnat S. aureus, and the mechanism may be the inhibition of growth and intracellular invasion, and alteration of antibiotic-resistance level of antibiotic-resistant S. aureus.

Berberine의 항생제 내성 황색포도구균에 대한 항미생물 효과

김해경,차정단,유용욱,김강주,이동근 원광대학교 생체재료·매식연구소 2000 원광생체재료·매식 Vol.9 No.1

Antibiotic-resistant Staphylococcus aureus (S. aureus) emerged in many parts of the world and increased complex clinical problems. Therefore, new agents are needed to treat to antibiotic-resistant S. aureus. Some natural products are candidates of new antibotic substances. Previous reports showed that the extract of Coptidis Rhizoma and Phellodendri Cortex have antimicrobial effects. The main antibacterial substance of Coptidis Rhizoma and Phellodendri Cortex is berberine. In this study, we examined the antimicrobial activity of berberine to antibiotic-resistant S. aureus. We isolated the 13 strains of antibiotic-resistant S. aureus from the oral cavity of healthy children in a preschool, and investigated the antimicrobial activity of berberine. We examined the effects of berberine on the level of resistance to antibiotic-resistant S. aureus. We observed that the invasion of S. aureus into human gingival fibroblasts was blocked by berberine. Berberine inhibited the growth of antibiotic-resistant. S. aureus as a dose dependent maner. Minimum inhibition concentration (MIC) of berberine against antibiotic-resistant S. aureus was 50-100 ㎍/ml. The inhibition of S. aureus invasion was depended on the concentration of berberine in human gingival fibroblasts. The MIC of ampicillin against antibiotic-resistant S. aureus was 32 ㎍/ml. However, the MIC of ampicillin against antibiotic-resitant S. aureus in the presence of 50 ㎍/ml berberine was 1 ㎍/ml. These data indicate that the combination with berberine and β-lactam antibiotics resulted in the synergistic reaction. These results suggest that the berberine may have the antimicrobial activities to antibiotic-resistant. S. aureus, and the mechanism may be the inhibition of growth and intracellular invasion. and alteration of antibiotic-resistance level of antibiotic-resistant S. aureus.

An Inverse Perspective Mapping Technique Based on a Virtual-Vertical Plane Model for Obstacle Detection Using Mono Camera

Dong-Hoon Yi,Tae-Jae Lee,Dong-Il “Dan” Cho 제어로봇시스템학회 2015 제어로봇시스템학회 국제학술대회 논문집 Vol.2015 No.10

This paper presents an improved inverse perspective mapping (IPM) technique based on a virtual-vertical plane model for obstacle detection. The conventional IPM based obstacle detection has a problem that it is difficult to determine whether the IPM based detected pixels are from real obstacle or caused by noise. Small errors in model, unknown camera motion, and colorful patterns of ground cause noises which are actually the ground region that is detected as an obstacle. It can be interpreted as false positives. Furthermore, there are few detections on homogeneous patterned obstacles such as an unicolor box. This work proposes a new simple method for distinguishing the noise and real obstacle in an image when IPM based detection method is used. A vertical plane model based detected pixel is checked after the IPM based detection. The results show that the real obstacles including a homogeneous patterned unicolor box are detected as obstacles, and A4-size black paper on a floor which is not an obstacle is not detected as an obstacle.

Response to Interferon Therapy Affects the Occurrence of Hepatocellular Carcinoma in Chronic Hepatitis B

( Dan Bi Lee ),( Young Hwa Chung ),( Se Hwan Lee ),( Yoon Seon Lee ),( Hyun Ju Min ),( Dong Dae Seo ),( Kang Mo Kim ),( Young Suk Lim ),( Han Chu Lee ),( Eun Sil Yu ),( Yung Sang Lee ),( Dong Jin Suh 대한간학회 2007 Clinical and Molecular Hepatology(대한간학회지) Vol.13 No.3(S)

GPR78 promotes lung cancer cell migration and metastasis by activation of Gαq-Rho GTPase pathway

( Dan-dan Dong ),( Hui Zhou ),( Gao Li ) 생화학분자생물학회(구 한국생화학분자생물학회) 2016 BMB Reports Vol.49 No.11

GPR78 is an orphan G-protein coupled receptor (GPCR) that is predominantly expressed in human brain tissues. Currently, the function of GPR78 is unknown. This study revealed that GPR78 was expressed in lung cancer cells and functioned as a novel regulator of lung cancer cell migration and metastasis. We found that knockdown of GPR78 in lung cancer cells suppressed cell migration. Moreover, GPR78 modulated the formation of actin stress fibers in A549 cells, in a RhoA- and Rac1-dependent manner. At the molecular level, GPR78 regulated cell motility through the activation of Gαq-RhoA/ Rac1 pathway. We further demonstrated that in vivo, the knockdown of GPR78 inhibited lung cancer cell metastasis. These findings suggest that GPR78 is a novel regulator for lung cancer metastasis and may serve as a potential drug target against metastatic human lung cancer. [BMB Reports 2016; 49(11): 623-628]

Afocal Optical Flow Sensor for Mobile Robot Odometry

Dong-Hoon Yi,Tae-Jae Lee,Dong-Il “Dan” Cho 제어로봇시스템학회 2015 제어로봇시스템학회 국제학술대회 논문집 Vol.2015 No.10

This paper presents an application of an afocal optical flow sensor (AOFS) for mobile robot navigation. The OFS-based odometry is advantageous for mobile robots because it is not affected by wheel slippage. However, the changes in vertical height result in systematic errors when estimating the moving distance of robots moving on uneven surfaces. This paper presents an application of an AOFS module to improve this vertical-height sensitivity. To evaluate the proposed system performance, two kinds of experiments are performed: on a bare laminate floor and on the laminate floor that is partially covered with an area carpet. The test area is 150 cm x 150 cm size. For the bare laminate floor case where no slip occurs, the localization error of the developed system is 0.2%. The error using a conventional OFS module, encoders, and gyroscope combination is also 0.2%. For the case where the laminate floor is partially covered with an area carpet, the localization error of the developed system is 0.7%. The error using a conventional OFS module, encoders, and the gyroscope is 2.9%.

Response of Grafted Watermelon Leaf Proteome Changes to Rootstock Genotypes

Dong Dong Yang,Hong Mei Du,Dan Feng Huang 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Microfabrication Methods for Biodegradable Polymeric Carriers for Drug Delivery System Applications: A Review

Dong-Il Dan Cho,Hyung Jung Yoo Institute of Electrical and Electronics Engineers 2015 Journal of microelectromechanical systems Vol. No.

<P>A drug delivery system is used for targeting drugs to specific cells. Various drug carriers, that also reduce the side effects of unbound drugs, have been introduced and commercialized in the pharmaceutical field. Among them, synthetic biodegradable polymers have received much attention attributed to their low toxicity, controllable biodegradation rates, manufacturability, and low costs. This paper reviews the salient characteristics of biodegradable polymers as drug carriers and their microfabrication methods. The reviewed microfabrication methods include laser micromachining, rapid prototyping, replication, emulsification, microfluidic fabrication, and X-ray-lithography-based methods. For these microfabrication methods, critical dimensions, feature variety, solvent compatibility, production throughput, and tooling requirements are also summarized.</P>