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Bui Thi Thuy Luyen,BUIHUU TAI,Nguyen Phuong Thao,이상현,장해동,이영미,김영호 한국응용생명화학회 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.5
Antioxidant and anti-osteoporosis activities of extractsand chemical constituents from the whole plant of Euphorbiamaculata were investigated. The MeOH extract, as well as EtOAcand H2O fractions (10.0 μg/mL), exhibited potent antioxidantactivities. Their oxygen radical absorbance capacity and cupricion reducing antioxidant capacity values were 27.07±0.31 to28.47±0.36 and 43.86±0.26 to 46.67±0.34 fold higher than thoseof 1.0 μM Trolox, respectively. The MeOH extract and EtOAcfraction (at 10.0 μg/mL) also significantly suppressed excessivebone resorption by osteoclasts with tartrate-resistant acid phosphatase(TRAP) activity values of 154.90±4.25 and 163.95±9.77%,respectively. Bioassay guided isolation of the EtOAc and H2Ofractions afforded 19 known compounds (1−19). Of these,compounds 18, and 13−15 showed good antioxidant activitybased on peroxyl radical-scavenging and reducing capacity assays,whereas compounds 1, 4, 7, and 14 showed the most significantinhibitory effect with TRAP activity values ranging from 121.31±1.41 to 110.00±3.74% relative to the control.
Bui Thi Thuy Luyen,Nguyen Phuong Thao,Bui Huu Tai,임지영,기현희,김대기,이영미,김영호 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.6
In this report, we investigated the anti-obesityeffect of wheat sprouts and their component compounds. Twenty compounds (1–20) were isolated from Triticumaestivum. Among them, glycolipids 1–5 were determinedfor the first time from T. aestivum and its sprouts. TheHPLC analysis demonstrated that compounds 1–3, 5, 8, 12,and 14 were major peak in the HPLC chromatogram of theactive fraction. The effects of the compounds on lipidaccumulation were assessed at concentrations ranging from1.0 to 100 lM. At concentration of 10.0 lM, compounds1–7, 10-15, and 17–19 significantly decreased lipidaccumulation in 3T3-L1 preadipocytes. Glycolipids 1, 2,and phenolic 17 significantly reduced lipid accumulation inthe differentiated adipocytes in a concentration-dependentmanner. Quantitative analysis based on measurement of theoptical density of Oil Red O indicated that, at 100 lM,compounds 1, 2, and 17 reduced lipid accumulation by 41,37, and 48 %, respectively, compared with the positive control.
Soluble Epoxide Hydrolase Inhibitory Activity from Euphorbia supina Rafin
Bui Thi Thuy Luyen,NGUYEN PHUONG THAO,BUIHUU TAI,Le Duc Dat,김지은,양서영,권세욱,이영미,김영호 한국생약학회 2015 Natural Product Sciences Vol.21 No.3
In our search for natural soluble epoxide hydrolase (sEH) inhibitors from plants, an extract of the dried whole plants of Euphorbia supina Rafin was found to significantly inhibit sEH activity in vitro. Phytochemical investigation of E. supina resulted in isolation of 17 compounds (1 - 17), including triterpenes (1 -4), phenolic compounds (5 - 8), and flavonoid derivatives (9 - 17). The structures of the isolated compounds were established mainly by extensive analysis of the 1D and 2D NMR, and MS data. All of the isolated compounds were evaluated for their sEH inhibitory activity. Among the isolated phenolic compounds, 8 was identified as a significant inhibitor of sEH, with an IC50 value of 15.4 ± 1.3 mM. Additionally, a kinetic analysis of isolated compounds (2, 5, 8 - 11, 13, and 17) indicated that the inhibitory effects of flavonoid derivatives 10 and 11 were of mixed-type, with inhibitory constants (Ki) ranging from 3.6 ± 0.8 to 21.8 ± 1.0 mM, whereas compounds 2, 5, 8, 9, 13, and 17 were non-competitive inhibitors with inhibition Ki values ranging from 3.3 ± 0.2 to 39.5 ± 0.0 mM.
Tai, Bui Huu,Nhut, Nguyen Duy,Nhiem, Nguyen Xuan,Quang, Tran Hong,Thanh Ngan, Nguyen Thi,Thuy Luyen, Bui Thi,Huong, Tran Thu,Wilson, Jennifer,Beutler, John A.,Ban, Ninh Khac,Cuong, Nguyen Manh,Kim, Yo Informa Healthcare 2011 PHARMACEUTICAL BIOLOGY Vol.49 No.10
<P><I>Context</I>: Acquired immune deficiency syndrome (AIDS) is a severe pandemic disease especially prevalent in poor and developing countries. Thus, developing specific, potent antiviral drugs that restrain infection by human immunodeficiency virus type 1 (HIV-1), a major cause of AIDS, remains an urgent priority.</P><P><I>Objective</I>: This study evaluated 32 extracts and 23 compounds from Vietnamese medicinal plants for their inhibitory effects against HIV-1 ribonuclease H (RNase H) and their role in reversing the cytopathic effects of HIV.</P><P><I>Materials and methods</I>: The plants were air-dried and extracted in different solvent systems to produce plant extracts. Natural compounds were obtained as previously published. Samples were screened for RNase H inhibition followed by a cytopathic assay. Data were analyzed using the Microsoft Excel.</P><P><I>Results and discussion</I>: At 50 μg/mL, 11 plant extracts and five compounds inhibited over 90% of RNase H enzymatic activity. Methanol extracts from <I>Phyllanthus reticulatus</I> and <I>Aglaia aphanamixis</I> leaves inhibited RNase H activity by 99 and 98%, respectively, whereas four extracts showed modest protection against the cytopathic effects of HIV.</P><P><I>Conclusion</I>: The screening results demonstrated that the butanol (BuOH) extract of <I>Celastrus orbiculata</I> leaves, methanol (MeOH) extracts of <I>Glycosmis stenocarpa</I> stems, <I>Eurya ciliata</I> leaves, and especially <I>P. reticulatus</I> leaves showed potential RNase H inhibition and protection against the viral cytopathic effects of HIV-1. Further chemical investigations should be carried out to find the active components of these extracts and compounds as potential anti-HIV drug candidates.</P>
Anti-osteoporotic and Antioxidant Activities by Rhizomes of Kaempferia parviflora Wall. ex Baker
NGUYEN PHUONG THAO,Bui Thi Thuy Luyen,이상현,장해동,김영호 한국생약학회 2016 Natural Product Sciences Vol.22 No.1
In this report, we investigated the antioxidant (peroxyl radical-scavenging and reducing capacities) and anti-osteoporotic activities of extracts and isolated constituents (1 - 16) from the rhizomes of Kaempferia parviflora Wall. ex Baker on pre-osteoclastic RAW 264.7 cells. Compound 5 exhibited significant peroxyl radicalscavenging capacity, with TE value of 8.47 ± 0.52 μM, while compound 13 showed significant reducing capacity, with CUPRAC value of 5.66 ± 0.26 μM, at 10.0 μM. In addition, flavonoid compounds 2, 4, 6, 8, 10, 12, and terpene compound 15 showed significant inhibition of tartrate-resistant acid phosphatase (TRAP) in NF-κB ligand-induced osteoclastic RAW 264.7 cells, with values ranging from 16.97 ± 1.02 to 64.67 ± 2.76%. These results indicated that K. parviflora could be excellent sources for the antioxidant and anti-osteoporotic traditional medicinal plants.
Nguyen Phuong Thao,Bui Thi Thuy Luyen,Chau Ngoc Diep,Bui Huu Tai,김은지,강희경,이상현,장해동,Nguyen The Cuong,Nguyen Van Thanh,Nguyen Xuan Cuong,Nguyen Hoai Nam,Chau Van Minh,김영호 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.4
This study performed phytochemical and bioactiveassessments of the mangrove Lumnitzera racemosaWilld. leaves. Bioassay-guided fractionation of the methanolicextracts led to the identification of thirty-six compounds(1–36), their structures were elucidated using detailed NMRspectroscopic and MS analysis. The extracts, fractions, andthe isolated compounds were screened for potential antioxidantand cytotoxic activities. Antioxidant assays wereperformed using peroxyl radical-scavenging and reducingassays, whereas cytotoxicity was measured using MTTassays in HL-60 and Hel-299 cell lines. The methanolicextract, CH2Cl2 and n-BuOH fractions (10.0 lg/mL)exhibited potent antioxidant activity, with Trolox equivalent(TE) values of 24.94 ± 0.59, 28.34 ± 0.20, and27.09 ± 0.37 (lM), respectively. In addition, the isolatedcompounds exerted cytotoxic effects in a dose-dependentmanner; compounds 1 and 14 exhibited the most potentcytotoxicity in HL-60 cells, with IC50 values of 0.15 ± 0.29and 0.60 ± 0.16 lM, respectively. To clarify the mechanism(s) behind these cytotoxic effects, we measured thetime-dependent changes in apoptotic markers including thecondensation and fragmentation of nuclear chromatin, andthe downregulation of p-ERK1/2, p-AKT, and c-Myc levels.
Nguyen Phuong Thao,Bui Thi Thuy Luyen,Nguyen Thi Thanh Ngan,Le Duc Dat,Nguyen Xuan Cuong,Nguyen Hoai Nam,Phan Van Kiem,Seok Bean Song,Chau Van Minh,김영호 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.5
Peroxisome proliferator-activated receptors(PPARs) are ligand-activated transcription factors thatregulate the expression of multiple genes involved inmetabolic, anti-inflammatory, and developmental processes. This study evaluated the PPARs transactivationaleffects of thirteen cembranoid diterpenoids 1–13 from thesoft coral Lobophytum crassum, using PPAR-responsiveelements–luciferase reporter and GAL4–PPAR chimeraassays. All isolated compounds activated the transcriptionof PPARs in a dose-dependent manner, with EC50 valuesranging from 2.07 ± 1.73 to 130.20 ± 1.85 lM. Moreover,compounds 6–9 affected the transactivation of allthree PPAR types, PPARa, c, b(d), in a dose-dependentmanner, with EC50 values in a ranging from 11.92 ± 1.23to 122.50 ± 2.12 lM. These results provide a scientificrationale for further studies on the soft coral L. crassum andits diterpenoid constituents to develop medicinal productsagainst inflammatory and metabolic diseases.
윤영필,이현재,김영호,Bui Thi Thuy Luyen,홍장희,이충재 한국생약학회 2015 Natural Product Sciences Vol.21 No.1
In this study, we investigated whether cynaroside, cynarin and linarin derived from Chrysanthemum indicum L. affect the secretion, production and gene expression of MUC5AC mucin in airway epithelial cells. Confluent NCI-H292 cells were pretreated with cynaroside, cynarin or linarin for 30 min and then stimulated with PMA (phorbol 12-myristate 13-acetate) for 24 h. The MUC5AC mucin gene expression, mucin protein production and secretion were measured by RT-PCR and ELISA, respectively. Effect of linarin on EGF (epidermal growth factor) - or TNF-a (tumor necrosis factor-a)-induced MUC5AC mucin gene expression and mucin protein production was also examined. The results were as follows: (1) Cynaroside and cynarin did not significantly affect PMA-induced MUC5AC mucin secretion from NCI-H292 cells. However, linarin decreased MUC5AC mucin secretion; (2) Cynaroside did not affect PMA-induced MUC5AC mucin production and gene expresion from NCI-H292 cells. However, cynarin and linarin inhibited the production and gene expression of MUC5AC mucin; (3) Linarin also inhibited the production and gene expression of MUC5AC mucin induced by EGF- or TNF-a from NCI-H292 cells. These results suggest that linarin can regulate the gene expression, production and secretion of mucin, by directly acting on airway epithelial cells.