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RISS 인기검색어
- 검색키워드
- 저자 : ( Ana R. Gomes ) (검색결과 3 건)
- 무료
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Original Article : Influence of P53 on the radiotherapy response of hepatocellular carcinoma
( Ana R. Gomes ),( Ana M. Abrantes ),( Ana F. Brito ),( Mafalda Laranjo ),( Joao E. Casalta Lopes ),( Ana C. Goncalves ),( Ana B. Sarmento Ribeiro ),( Maria F. Botelho ),( Jose G. Tralhao ) 대한간학회 2015 Clinical and Molecular Hepatology(대한간학회지) Vol.21 No.3
Background/Aims: Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide, and it has a poor prognosis and few therapeutic options. Radiotherapy is one of the most effective forms of cancer treatment, and P53 protein is one of the key molecules determining how a cell responds to radiotherapy. The aim of this study was to determine the therapeutic effi cacy of iodine-131 in three human HCC cell lines. Methods: Western blotting was used to measure P53 expression. The effects of radiotherapy with iodine-131 were assessed by using the clonogenic assay to evaluate cell survival. Flow cytometry was carried out to examine the effectsof iodine-131 on cell death, oxidative stress, reduced intracellular glutathione expression, the mitochondrial membrane potential, and the cell cycle. Results: The P53 protein was not expressed in Hep3B2.1-7 cells, was expressed at normal levels in HepG2 cells, and was overexpressed in HuH7 cells. P53 expression in the HuH7 and HepG2 cell lines increased after internal and external irradiation with iodine-131. Irradiation induced a decrease in cell survival and led to a decrease in cell viability in all of the cell lines studied, accompanied by cell death via late apoptosis/necrosis and necrosis. Irradiation with 131-iodine induced mostly cell-cycle arrest in the G0/G1 phase. Conclusions: These results suggest that P53 plays a key role in the radiotherapy response of HCC. (Clin Mol Hepatol 2015;21:257-267)
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Solar photocatalysis of a recalcitrant coloured effluent from a wastewater treatment plant
Vilar, Vitor J.P.,Gomes, Ana I.E.,Ramos, Vanessa M.,Maldonadob, Manuel I.,Boaventura, Rui A.R. Korean Society of Photoscience 2009 Photochemical & photobiological sciences Vol.8 No.5
A photocatalytic study of a coloured effluent from a wastewater treatment plant was carried out in a pilot plant using compound parabolic collectors (CPC) in order to find out the best conditions for colour removal, since the discharge limit for this parameter is not achieved after conventional wastewater treatment. The interaction between ultraviolet natural radiation and $TiO_2$ strongly enhanced the colour degradation rate. Different $TiO_2$ concentrations were tested and the optimum concentration achieved was $200\;mg\;L^{-1}$. The use of peroxydisulfate ($S_2{O_8}^{2-}$) as an additional electron scavenger gave a noticeable effect on colour and dissolved organic carbon (DOC) removal due to the formation of additional powerful oxidant species ($OH^{\cdot}$ and ${SO_4}^{{\cdot}-}$). However, hydrogen peroxide ($H_2O_2$) as additional oxidant was more efficient on colour degradation than $S_2{O_8}^{2-}$. The amount of energy necessary for the same colour removal (C/$C_0$ < 0.1) was 6, 14 and $80\;kJ_{UV}\;L^{-1}$, respectively, for the following systems using sunlight: $200\;mg\;L^{-1}$ $TiO_2$ + 5 mM $H_2O_2$, $200\;mg\;L^{-1}$ $TiO_2$ + 2 mM $S_2{O_8}^{2-}$ and $200\;mg\;L^{-1}$ $TiO_2$. The first-order kinetic constants (0.487, 0.207 and $0.053\;L\;kJ^{-1}$) and initial degradation rates (32.1, 10.0 and $2.2\;mg\;kJ^{-1}$) showed the same behaviour.
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Isolation of Surfactant-Resistant Pseudomonads from the Estuarine Surface Microlayer
( Louvado Antonio ),( Francisco J. R. C. Coelho ),( Patricia Domingues ),( Ana L. Santos ),( Newton C. M. Gomes ),( Adelaide Almeida ),( Angela Cunha ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.3
Bioremediation efforts often rely on the application of surfactants to enhance hydrocarbon bioavailability. However, synthetic surfactants can sometimes be toxic to degrading microorganisms, thus reducing the clearance rate of the pollutant. Therefore, surfactant-resistant bacteria can be an important tool for bioremediation efforts of hydrophobic pollutants, circumventing the toxicity of synthetic surfactants that often delay microbial bioremediation of these contaminants. In this study, we screened a natural surfactant-rich compartment, the estuarine surface microlayer (SML), for cultivable surfactant-resistant bacteria using selective cultures of sodium dodecyl sulfate (SDS) and cetyl trimethylammonium bromide (CTAB). Resistance to surfactants was evaluated by colony counts in solid media amended with critical micelle concentrations (CMC) of either surfactants, in comparison with non-amended controls. Selective cultures for surfactant-resistant bacteria were prepared in mineral medium also containing CMC concentrations of either CTAB or SDS. The surfactantresistant isolates obtained were tested by PCR for the Pseudomonas genus marker gacA gene and for the naphthalene-dioxygenase-encoding gene ndo. Isolates were also screened for biosurfactant production by the atomized oil assay. A high proportion of culturable bacterioneuston was tolerant to CMC concentrations of SDS or CTAB. The gacA-targeted PCR revealed that 64% of the isolates were Pseudomonads. Biosurfactant production in solid medium was detected in 9.4% of tested isolates, all affiliated with genus Pseudomonas. This study shows that the SML is a potential source of surfactant-resistant and biosurfactant-producing bacteria in which Pseudomonads emerge as a relevant group.
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