Analysis of genetic diversity in a collection of Plantago species: application of ISSR markers

Bagheri Motahareh,Heidari Bahram,Dadkhodaie Ali,Heidari Zahra,Daneshnia Negar,Richards Christopher M. 한국작물학회 2022 Journal of crop science and biotechnology Vol.25 No.1

The analysis of genetic diversity in medicinal plant species can greatly facilitate germplasm conservation and selection for use in breeding schemes. The aim of the present study was to assess the genetic diversity and diferentiation of eight Plantago species using inter simple sequence repeat (ISSR) markers. Thirty-one Plantago accessions belonging to eight species were collected from Iran environments. Polymorphisms in the DNA of Plantago accessions were analyzed using 25 ISSR primers. The data for 286 polymorphic bands indicated that the ISSR primers each amplifed 5–21 polymorphic amplicons that were 100–3000 bp in size. The mean polymorphism was 83.83% and fve primers showed higher polymorphism. The polymorphism information content (PIC) ranged from 0.1103 to 0.3829 with a mean of 0.2727. Accessions in P. amplexicaulis and P. pysillum represented the highest Nei’s and Shannon’s genetic diversity, while the lowest was obtained for P. lagopus. The phenetic network generated by the Neighbor-Net algorithm showed moderate confict and splitting of the eight species. Principal coordinate analysis (PCoA) results showed the separation of accessions of the eight species. The results suggest that ISSR markers can diferentiate Plantago species and that information on genetic diversity may assist in pre-breeding evaluations and Plantago germplasm conservation.

Magnetic Resonance Imaging and Sonographic Features before and after Surgery in Carpal Tunnel Syndrome: Association with Clinical Findings

Mahla Daliri,Motahareh Ebrahimnejad,Samane Najafi,Behzad Aminzadeh,Maryam Emadzadeh,Department of Neurosurgery, Shahid Beheshti University of Medical Scie,Ali Moradi 대한정형외과학회 2022 Clinics in Orthopedic Surgery Vol.14 No.4

Background: The interest in ultrasonography (US) and magnetic resonance imaging (MRI) assessment of the patients with carpal tunnel syndrome (CTS) is growing. This paper aimed to find the correlation of postoperative changes in these modalities’ parameters with clinical outcomes. Methods: Boston CTS questionnaire-symptom severity (BQ-SS), Boston CTS questionnaire-functional status (BQ-FS), and visual analog scale (VAS) questionnaires (for pain, paresthesia, and grip weakness assessment) were used to evaluate clinical outcomes. Various imaging parameters of the median nerve and carpal tunnel were evaluated using US and MRI at two levels of the hook of the hamate (distal) and the pisiform (proximal) once preoperatively and then 3 months postoperatively. Corresponding US and MRI parameter measures were compared, and correlational analysis was performed between alteration of imaging findings and changes in clinical parameters postoperatively. Results: Patients’ functional status (BQ-FS score) was positively correlated with the nerve width both in US and MRI at the proximal level (r = 0.457 and r = 0.453, respectively) and also with the MRI nerve circumference at the distal level (r = –0.482). Correlation between paresthesia and the nerve width was notable in MRI at the distal hook of the hamate level (r = –0.403). Grip weakness VAS score was correlated with the nerve width-to-height ratio (WHR) in US at the distal level (r = 0.432). Conclusions: Changes in US and MRI parameters of the median nerve width, circumference, and WHR were associated with clinical changes in patients with CTS after surgery.

Weathering of Palygorskite in the Rhizosphere of Rapeseed

Farhad Khormali,Motahareh Sadat Hashemi Rakava,Esmaeil Dordipour,Soheila Ebrahimi 한국토양비료학회 2014 한국토양비료학회 학술발표회 초록집 Vol.2014 No.6

Muscle Strength and Biochemical Markers as Predictors of Depression in Hemodialysis Patients: A Cross-Sectional Study

Soudabeh Zare,Motahareh Hasani,M. Dulce Estêvão,Rahim Tahmasebi,Leila Azadbakht,Farzad Shidfar,Javad Heshmati,Somayeh Ziaei 한국임상영양학회 2023 Clinical Nutrition Research Vol.12 No.4

Patients with chronic renal failure, many of which treated with hemodialysis, present a high prevalence of impaired muscle strength which suggest that muscle mass parameters may be used as markers for changes in muscle in these patients. Measurement of handgrip strength (HGS) is a common, simple, and quick measure of muscle function an indicator of overall muscle strength which has been associated with physical activity and several anthropometric traits. Intercellular adhesion molecule-1 (ICAM-1) and insulin-like growth factor-1 (IGF-1) are biochemical markers associated with inflammatory processes which are a common consequence of dialysis. Additionally, hemodialysis patients frequently present signs of malnutrition and depression. This cross-sectional study aimed to evaluate if muscle and biochemical markers could be used to predict the risk of depression in hemodialysis patients. Several anthropometric parameters, nutrient intake, depression state and the serum levels of ICAM-1 and IGF-1 were determined and Pearson’s correlation coefficient and/or Spearman’s correlation coefficient were used to test the correlation between them. Our results do not show a correlation between HGF, IGF-1 and ICAM-1 with the depression status of the patients, but mid-arm muscle circumference (MAMC) was statistically and positively correlated with depression. Additionally, ICAM-1 levels were negatively correlated with HGS, MAMC, and IGF-1. Overall, the results of the present study suggest that HGS may be used as an indicator of cardiovascular diseases and MAMC may be a good predictor of the level of depression in hemodialysis patients, although further studies are required.

Enrolment Phase Results of the Tabari Cohort Study: Comparing Family History, Lipids and Anthropometric Profiles Among Diabetic Patients

Mahmood Moosazadeh,Mohammad Khademloo,Kaveh Jafari,Motahareh Kheradmand,Zahra Kashi,Mohsen Aarabi,Adeleh Bahar,Mahdi Afshari 질병관리본부 2019 Osong Public Health and Research Persptectives Vol.10 No.5

Objectives: Different factors are responsible for the silent epidemic of diabetes mellitus in developing and developed countries. This study aimed to determine the role of demographic factors, lipid profile, family history (the estimation of genetic association) and anthropometric factors on diabetes onset. Methods: Data from the enrolment phase of the Tabari Cohort study was applied for this study and included 10,255 participants aged between 35-70 years. Anthropometric variables were measured by trained staff using standard tools. Blood specimens were collected for lipid profile and blood glucose measurements. Data analyses were performed using SPSS version 24, with univariate and multivariate logistic regression. Results: The prevalence of diabetes mellitus was estimated to be 17.2% in the cohort population, 15.6% in men, and 18.3% in women. The adjusted odds ratios (95% confidence intervals) for age groups 40- 49, 50-59 and over 60 were 2.58 (2.20-3.69), 5.80 (4.51-7.48) and 8.72 (6.67-11.39), respectively. In addition, the odds ratios (95% confidence intervals) for 2 (or more), and 1 affected family member were 4.12 (3.55-4.90) and 2.34 (2.07-2.65), respectively. Triglyceride concentrations more than 500, and abnormal high-density lipoprotein levels increased the odds of diabetes mellitus by 3.29- and 1.18- fold, respectively. Conclusion: The current study showed that old age and a family history were strong predictors for diabetes mellitus.

Epidemiological and Clinical Features of People with Malta Fever in Iran: A Systematic Review and Meta-Analysis

Mahmood Moosazadeh,Roja Nikaeen,Ghasem Abedi,Motahareh Kheradmand,Saeid Safiri 질병관리본부 2016 Osong Public Health and Research Persptectives Vol.7 No.3

Objectives: Numerous studies have reported the epidemiological and clinical features of Malta fever incidence in Iran. Review and synthesis of the related literature through meta-analysis can provide an appropriate measurement for aforementioned indices. Therefore, the present study aimed to determine the epidemiological and clinical features of people with Malta fever in Iran. Methods: The required documents were obtained through searching national and international databases. In each study, standard deviation of the indices was calculated using binomial distribution formulas. Finally, the heterogeneity index was determined between studies using Cochran (Q) and I2 tests. Results: Combining the results of 47 articles in the meta-analysis indicated that 57.6% (55.02-60.1%) and 42.3% (49.8-44.9%) of the patients were male and female, respectively. Most of the patients lived in rural areas; 68.4% (63.6 -73.2%) compared to 31.4% (26.7-36.3%). In addition, 20.8% (17.4-24.2%) of the patients were ranchers and farmers, 16.9% (14.5-19.4%) were students, and 31.6% (27-36.2%) were housewives. Of the patients studies, 50.5% (35.6-65.2%) experienced contact with animals and 57.1% (46.4-67.9%) used unpasteurized dairy products. Fever, joint pain, and sweating were detected among 65.7% (53.7 -77.8%) and 55.3% (44.4-66.2%), respectively. Conclusion: The present study revealed that the frequency of male patients with brucellosis was considerably more than that of female patients. The number of patients with Malta fever in rural areas was significantly more than in urban areas. High-risk behavior, unprotected contact with animals, and using unpasteurized dairy products were among the most significant factors affecting Malta fever incidence in Iran. Fever, joint pain, and sweating were detected among most of the patients with Malta fever.

Orbital Exenteration: A 23-year Report

abolfazl kasaee,mansooreh jamshidian-Tehrani,bahram eshraghy,kambiz ameli,shahbaz nekoozadeh,motahareh sadeghi 대한안과학회 2019 Korean Journal of Ophthalmology Vol.33 No.4

Purpose: Orbital exenteration is a psychologically and anatomically disfiguring procedure which indicated insome patients with malignant or progressive diseases of orbital and periorbital area. In this study, we reviewed176 patients that underwent orbital exenteration. Methods: This was a retrospective study of medical records from all patients who underwent orbital exenterationfrom March 1991 to March 2014 in oculoplastic department at an eye care center. Demographic data,diagnosis, site of primary involvement and technique of surgery were determined in patients. Results: One hundred seventy-six cases of orbital exenteration were included that had documented histopathology. The age of patients ranged from 1 to 91 years (mean age ± standard deviation, 55.43 ± 27 years). Ninety-seven (55.11%) males and 79 (44.88%) females were included. Fifteen different tumors were identified. Themost common indication was patients with basal cell carcinoma 49 (28%) followed by 41 (23.5%) squamouscell carcinomas, 35 (20%) retinoblastoma, and 13 (7%) adenoid cystic carcinomas. In total, adnexal malignancieswere the most common tumors, secondarily involving the orbit. Eyelids 89 (50.5%) and the globe 43 (24%)were the most frequent site of involvement. Three types of exenteration were performed, based on availabledata of 129 operation sheets, 46 (35.7%) subtotal, 62 (48.1%) total, and 21 (16.3%) cases of extensive exenterations. In total 97 cases were evaluated pathologically for perineural involvement, of which perineural invasionwas noted in 9 (7%) reports. Conclusions: Frequency of exenteration in our center has increased in past 3 years and the majority of caseswere eyelid basal cell carcinoma. Patient education considering periocular lesions can help in earlier diagnosisof malignant lesions and therefore reducing the number of exenteration.

Age of Natural Menopause and Related Factors among the Tabari Cohort

Marzieh Zamaniyan,Mahmood Moosazadeh,Sepideh Peyvandi,Kaveh Jaefari,Reza Goudarzi,Mahdi Moradinazar,Motahareh Kheradmand 대한폐경학회 2020 대한폐경학회지 Vol.26 No.1

Objectives: The age of menopause is affected by several factors. In this study we aimed to identify the age of natural menopause and its related factors in a large-scale population-based cohort in Iran. Methods: In this study, a subset of data collected during the enrollment phase of the Tabari cohort study was utilized. Reproductive history and other related data were collected using a structured questionnaire. Blood samples were obtained from all participants. Data were analyzed using chi-square test, independent t test, and ANOVA as well as a multivariate linear regression model. Results: Among participants of the Tabari cohort, 2,753 were menopausal women. The mean age of natural and induced menopause was 49.2 ± 4.7 and 43.2 ± 6.4 years, respectively (P = 0.001). The number of pregnancies, duration of breastfeeding, level of education, residency, presence of thyroid disease, and body mass index affected the age of menopause. After adjustments for confounding variables, the number of pregnancies remained significantly associated with late menopause. Conclusions: The age of natural menopause in this study was similar to that in other studies, and the number of pregnancies was positively associated with the age of menopause after adjustments for confounding variables.

Effect of Selenium Supplementation on Expression of SIRT1 and PGC-1α Genes in Ulcerative Colitis Patients: a Double Blind Randomized Clinical Trial

( Maryam Khazdouz ),( Naser Ebrahimi Daryani ),( Foroogh Alborzi ),( Mir Hadi Jazayeri ),( Farnaz Farsi ),( Motahareh Hasani ),( Javad Heshmati ),( Farzad Shidfar ) 한국임상영양학회 2020 Clinical Nutrition Research Vol.9 No.4

Selenium (Se) supplementation may decrease the severity of ulcerative colitis (UC) through the activation of genes responsible for immune modulation. The present research was aimed to assess the effect of Se supplementation on the expression of silent information regulator 1 (SIRT1) and peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α) in UC patients. In a double-blind randomized parallel clinical trial, 100 patients with mild-to-moderate active UC met inclusion criteria and divided into 2 groups of treatment (50 patients received selenomethionine [200 μg daily]) and placebo (50 patients received placebo [1 capsule daily]) for 10 weeks. The expression rates of SIRT1 and PGC-1α were examined in the peripheral blood mononuclear cell (PBMC) using the real-time polymerase chain reaction. There was no considerable difference in the mean of baseline demographic and clinical characteristics between groups. Also, there were no significant differences in total energy intake, macronutrients, and micronutrients between groups. The SIRT1 gene expression in the Se group was significantly increased compared to the placebo (p < 0.001). An increase in the expression of the PGC-1α gene in the Se group was not statistically significant. It seems that Se supplementation caused a significant decrease in the inflammatory response of the colon by a significant increase in the expression of the SIRT1 gene.