A novel practical method to predict anterior cruciate ligament hamstring graft size using preoperative MRI

( Zi Qiang Glen Liau ),( Matthew Song Peng Ng ),( Shawn Shao En Low ),( Brian Zhaojie Chin ),( James Hoi Po Hui ),( Fareed Husain Yusuf Kagda ) 대한슬관절학회 2024 대한슬관절학회지 Vol.36 No.-

Background Predicting hamstring graft size preoperatively for anterior cruciate ligament (ACL) reconstruction is important for preempting an insufficient diameter in graft size intraoperatively, possibly leading to graft failure. While there are multiple published methods using magnetic resonance imaging (MRI) picture archiving and communication systems (PACS), most are not feasible and practical. Our study aims to (1) practically predict the ACL hamstring graft size in a numerically continuous manner using the preoperative MRI from any native MRI PACS system, (2) determine the degree of correlation between the predicted and actual graft size, and (3) determine the performance of our prediction method if we define an adequate actual graft size as ≥ 8 mm. Methods A retrospective review of 112 patients who underwent primary ACL reconstruction with quadrupled hamstring semitendinosus-gracilis grafts at a tertiary institution was conducted between January 2018 and December 2018. Graft diameter can be predicted in a numerically continuous manner as √[2*(AB + CD)], where A and B are the semitendinosus cross-sectional length and breath, respectively, and C and D are the gracilis cross-sectional length and breath, respectively. Results A moderately positive correlation exists between the predicted and actual graft diameter (r = 0.661 and p < .001). Our method yields a high specificity of 92.6% and a moderate sensitivity of 67.2% if we define an adequate actual graft size as ≥ 8 mm. An area under receiver-operating characteristic curve shows good discrimination (AUC = 0.856). Conclusions We present a practical method to predict the ACL hamstring graft size with high specificity using preoperative MRI measurements.

MEASUREMENT OF DYNAMIC SURFACE TENSION OF AQUEOUS PROTEIN SOLUTIONS BY THE DROP-VOLUME TECHNIQUE

Zi Qiang Zhang,Yasuhiko H. Mori 대한기계학회 1996 KSME/JSME THERMAL and FLUID Engineering Conference Vol.2 No.1

Effect of Trichostatin A on Anti HepG2 Liver Carcinoma Cells: Inhibition of HDAC Activity and Activation of Wnt/β-Catenin Signaling

Shi, Qing-Qiang,Zuo, Guo-Wei,Feng, Zi-Qiang,Zhao, Lv-Cui,Luo, Lian,You, Zhi-Mei,Li, Dang-Yang,Xia, Jing,Li, Jing,Chen, Di-Long Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.18

Purpose: To investigate the effect of deacetylase inhibitory trichostatin A (TSA) on anti HepG2 liver carcinoma cells and explore the underlying mechanisms. Materials and Methods: HepG2 cells exposed to different concentrations of TSA for 24, 48, or 72h were examined for cell growth inhibition using CCK8, changes in cell cycle distribution with flow cytometry, cell apoptosis with annexin V-FTIC/PI double staining, and cell morphology changes under an inverted microscope. Expression of ${\beta}$-catenin, HDAC1, HDAC3, H3K9, CyclinD1 and Bax proteins was tested by Western blotting. Gene expression for ${\beta}$-catenin, HDAC1and HDAC3 was tested by q-PCR. ${\beta}$-catenin and H3K9 proteins were also tested by immunofluorescence. Activity of Renilla luciferase (pTCF/LEF-luc) was assessed using the Luciferase Reporter Assay system reagent. The activity of total HDACs was detected with a HDACs colorimetric kit. Results: Exposure to TSA caused significant dose-and time-dependent inhibition of HepG2 cell proliferation (p<0.05) and resulted in increased cell percentages in G0/G1 and G2/M phases and decrease in the S phase. The apoptotic index in the control group was $6.22{\pm}0.25%$, which increased to $7.17{\pm}0.20%$ and $18.1{\pm}0.42%$ in the treatment group. Exposure to 250 and 500nmol/L TSA also caused cell morphology changes with numerous floating cells. Expression of ${\beta}$-catenin, H3K9and Bax proteins was significantly increased, expression levels of CyclinD1, HDAC1, HDAC3 were decreased. Expression of ${\beta}$-catenin at the genetic level was significantly increased, with no significant difference in HDAC1and HDAC3 genes. In the cytoplasm, expression of ${\beta}$-catenin fluorescence protein was not obvious changed and in the nucleus, small amounts of green fluorescence were observed. H3K9 fluorescence protein were increased. Expression levels of the transcription factor TCF werealso increased in HepG2 cells following induction by TSA, whikle the activity of total HDACs was decreased. Conclusions: TSA inhibits HDAC activity, promotes histone acetylation, and activates Wnt/${\beta}$-catenin signaling to inhibit proliferation of HepG2 cell, arrest cell cycling and induce apoptosis.

Glyceraldehyde-3-Phosphate Dehydrogenase, an Immunogenic Streptococcus equi ssp. zooepidemicus Adhesion Protein and Protective Antigen

( Qiang Fu ),( Zi Gong Wei ),( Xiao Hong Liu ),( Ping Ping Xiao ),( Zhao Hui Lu ),( Yao Sheng Chen ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.4

Streptococcus equi ssp. zooepidemicus (Streptococcus zooepidemicus, SEZ) is an important pathogen associated with opportunistic infections of a wide range of species, including pigs and humans. The absence of a suitable vaccine makes it difficult to control SEZ infection. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) has been previously identified as an immunogenic protein using immunoproteomic techniques. In the present study, we confirmed that the sequence of GAPDH was highly conserved with other Streptococcus spp. The purified recombinant GAPDH could elicit a significant humoral antibody response in mice and confer significant protection against challenge with a lethal dose of SEZ. GAPDH could adhere to the Hep-2 cells, confirmed by flow cytometry, and inhibit adherence of SEZ to Hep-2 cells in an adherence inhibition assay. In addition, real-time PCR demonstrated that GAPDH was induced in vivo following infection of mice with SEZ. These suggest that GAPDH could play an important role in the pathogenesis of SEZ infection and could be a target for vaccination against SEZ.

Construction and Characterization of Vitreoscilla Hemoglobin (VHb) with Enhanced Peroxidase Activity for Efficient Degradation of Textile Dye

( Zi Dong Zhang ),( Wei Li ),( Hai Chao Li ),( Jing Zhangi ),( Yue Bin Zhang ),( Yu Feng Cao ),( Jian Zhang Ma ),( Zheng Qiang Li ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.9

Pollution resulting from the discharge of textile dyes into water systems has become a major global concern. Because peroxidases are known for their ability to decolorize and detoxify textile dyes, the peroxidase activity of Vitreoscilla hemoglobin (VHb) has recently been studied. It is found that VHb and variants of this enzyme show great promise for enzymatic decolorization of dyes and may play a role in achieving their successful removal from industrial wastewater. The level of VHb peroxidase activity correlates with two amino acid residues present within the conserved distal pocket, at positions 53 and 54. In this work, sitedirected mutagenesis of these residues was performed and resulted in improved VHb peroxidase activity. The double mutant, Q53H/P54C, shows the highest dye decolorization and removal efficiency, with 70% removal efficiency within 5 min. UV spectral studies of Q53H/P54C reveals a more compact structure and an altered porphyrin environment (λSoret = 413 nm) relative to that of wild-type VHb (λSoret = 406), and differential scanning calorimetry data indicate that the VHb variant protein structure is more stable. In addition, circular dichroism spectroscopic studies indicate that this variant’s increased protein structural stability is due to an increase in helical structure, as deduced from the melting temperature, which is higher than 90°C. Therefore, the VHb variant Q53H/P54C shows promise as an excellent peroxidase, with excellent dye decolorization activity and a more stable structure than wild-type VHb under high-temperature conditions.

A Hybrid Static Compensator for Dynamic Reactive Power Compensation and Harmonic Suppression

Jia-Qiang Yang,Lei Yang,Zi-peng Su 전력전자학회 2017 JOURNAL OF POWER ELECTRONICS Vol.17 No.3

This paper presents a combined system of a small-capacity inverter and multigroup delta-connected thyristor switched capacitors (TSCs). The system is referred to as a hybrid static compensator (HSC) and has the functions of dynamic reactive power compensation and harmonic suppression. In the proposed topology, the load reactive power is mainly compensated by the TSCs. Meanwhile the inverter is meant to cooperate with TSCs to achieve continuous reactive power compensation, and to filter the harmonics generated by nonlinear loads and the TSCs. First, the structure and mathematical model of the HSC are discussed Then the control method of the HSC is presented. An improved reduced order generalized integrator (ROGI)-based selective current control method is adopted in the inverter to achieve high-performance reactive and harmonic current compensation. Meanwhile, a switch control strategy is proposed to implement precise and fast switching of the TSCs and to avoid changing the time delay needed by the conventional switch strategy. Experiments are implemented on a 20 KVA HSC prototype and the obtained results verify the validity of the proposed HSC system.

ZNF217 is Overexpressed and Enhances Cell Migration and Invasion in Colorectal Carcinoma

Zhang, Zi-Chao,Zheng, Li-Qiang,Pan, Li-Jie,Guo, Jin-Xing,Yang, Guo-Shan Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.6

Background: To investigate the expression and clinical significance of zinc finger protein 217 (ZNF217) in human colorectal carcinoma (CRC). Materials and Methods: The expression of ZNF217 in 60 CRC tissues and matched tumor adjacent tissues, collected between January 2013 and June 2014, was assessed immunohistochemically. The relationship between the expression of ZNF217 and clinicopathlogical features was analyzed by Pearson chi-square test. In addition, siRNA was used to down-regulate the expression of ZNF217 in CRC cells. The effects of ZNF217 for cell migration and invasion were measured by wound healing assay and transwell assay, respectively. Results: The expression level of ZNF217 was significantly higher in CRC tissues than in tumor adjacent tissues (p<0.05), positively correlating with tumor size, lymphatic metastasis and advanced TNM stage (p<0.05). Down-regulation of ZNF217 in CRC cells could significantly suppress cell migration and invasion. Conclusions: ZNF217 is overexpressed in colorectal carcinoma tissues and is associated with tumor malignant clinicopathological features. ZNF217 may promote CRC progression by inducing cell migration and invasion.

A Hybrid Static Compensator for Dynamic Reactive Power Compensation and Harmonic Suppression

Yang, Jia-qiang,Yang, Lei,Su, Zi-peng The Korean Institute of Power Electronics 2017 JOURNAL OF POWER ELECTRONICS Vol.17 No.3

This paper presents a combined system of a small-capacity inverter and multigroup delta-connected thyristor switched capacitors (TSCs). The system is referred to as a hybrid static compensator (HSC) and has the functions of dynamic reactive power compensation and harmonic suppression. In the proposed topology, the load reactive power is mainly compensated by the TSCs. Meanwhile the inverter is meant to cooperate with TSCs to achieve continuous reactive power compensation, and to filter the harmonics generated by nonlinear loads and the TSCs. First, the structure and mathematical model of the HSC are discussed Then the control method of the HSC is presented. An improved reduced order generalized integrator (ROGI)-based selective current control method is adopted in the inverter to achieve high-performance reactive and harmonic current compensation. Meanwhile, a switch control strategy is proposed to implement precise and fast switching of the TSCs and to avoid changing the time delay needed by the conventional switch strategy. Experiments are implemented on a 20 KVA HSC prototype and the obtained results verify the validity of the proposed HSC system.

Perceptions of a night float system for intern doctors in an internal medicine program: an Asian perspective

Benjamin Yong-Qiang Tan,Nicholas Jinghao Ngiam,Zi Yun Chang,Sandra Ming Yien Tan,Xiayan Shen,Shao Feng Mok,Srinivas Subramanian,Shirley Beng Suat Ooi,Adrian Chin-Leong Kee 한국의학교육학회 2019 Korean journal of medical education Vol.31 No.3

Long duty hours have been associated with significant medical errors, adverse events, and physician “burn-out”. An innovative night float (NF) system has been implemented in our internal medicine program to reduce the negative effects of long duty hours associated with conventional full-call systems. However, concerns remain if this would result in inadequate training for interns. We developed a structured questionnaire to assess junior doctors’ perceptions of the NF system compared to full calls, in areas of patient safety, medical training, and well-being. Ninety-seven (71%) of the 137 doctors polled responded. Ninety-one (94%) felt the NF system was superior to the full call system. A strong majority felt NF was beneficial for patient safety compared to full call (94% vs. 2%, p<0.001). The NF system was also perceived to reduce medical errors (94% vs. 2%, p<0.001) and reduce physician “burn-out” (95% vs. 5%, p<0.001). Beyond being a practical solution to duty-hour limitations, there was a significant perceived benefit of the NF system compared to the full call in terms of overall satisfaction, patient safety, reducing medical errors and physician “burn-out”.

Evaluating the accuracy and relevance of ChatGPT responses to frequently asked questions regarding total knee replacement

( Siyuan Zhang ),( Zi Qiang Glen Liau ),( Kian Loong Melvin Tan ),( Wei Liang Chua ) 대한슬관절학회 2024 대한슬관절학회지 Vol.36 No.-

Background Chat Generative Pretrained Transformer (ChatGPT), a generative artificial intelligence chatbot, may have broad applications in healthcare delivery and patient education due to its ability to provide human-like responses to a wide range of patient queries. However, there is limited evidence regarding its ability to provide reliable and useful information on orthopaedic procedures. This study seeks to evaluate the accuracy and relevance of responses provided by ChatGPT to frequently asked questions (FAQs) regarding total knee replacement (TKR). Methods A list of 50 clinically-relevant FAQs regarding TKR was collated. Each question was individually entered as a prompt to ChatGPT (version 3.5), and the first response generated was recorded. Responses were then reviewed by two independent orthopaedic surgeons and graded on a Likert scale for their factual accuracy and relevance. These responses were then classified into accurate versus inaccurate and relevant versus irrelevant responses using preset thresholds on the Likert scale. Results Most responses were accurate, while all responses were relevant. Of the 50 FAQs, 44/50 (88%) of ChatGPT responses were classified as accurate, achieving a mean Likert grade of 4.6/5 for factual accuracy. On the other hand, 50/50 (100%) of responses were classified as relevant, achieving a mean Likert grade of 4.9/5 for relevance. Conclusion ChatGPT performed well in providing accurate and relevant responses to FAQs regarding TKR, demonstrating great potential as a tool for patient education. However, it is not infallible and can occasionally provide inaccurate medical information. Patients and clinicians intending to utilize this technology should be mindful of its limitations and ensure adequate supervision and verification of information provided.