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Zhiqian Pang,Zhuangzhi Zhou,Dedong Yin,Qiming Lv,Lixiang Wang,Xiao Xu,Jing Wang,Xiaobing Li,Xianfeng Zhao,Guanghuai Jiang,Jinping Lan,Lihuang Zhu,Songnian Hu,Guozhen Liu 한국식물학회 2013 Journal of Plant Biology Vol.56 No.6
Plant Bowman-Birk type bran trypsin inhibitors(BBTI) belong to a family of serine protease inhibitors thatinhibit trypsin activity and play roles in plant developmentand defense responses to both biotic and abiotic stresses. Inthis study, transgenic rice plants overexpressing BBTI4 (OXBBTI4)were generated. Reverse-transcription polymerasechain reaction and western blot (WB) analysis demonstratedthat the BBTI4 mRNA and protein levels were significantlyincreased in OX-BBTI4. Notably, two BBTI4 protein formswith different molecular weight (18 kD and 28 kD) wererevealed by WB analysis. In non-transgenic plants, BBTI4-28kD and BBTI4-18kD were mainly expressed in roots andleaves, respectively, while in transgenic OX-BBTI4 plants,both protein forms were expressed constitutively. Subcellularanalysis revealed that BBTI4 is localized in the cytosol. Moreover, Xanthomonas oryzae pv. oryzae (Xoo) inoculationexperiments demonstrated that transgenic OX-BBTI4 riceplants conferred partial but broad-spectrum Xoo resistance. InOX-BBTI4 transgenic rice plants, the expression of OsPR3 andOsPR10a proteins was induced and gradually increased afterXoo infection, while the expression of OsPR1a, OsPR1b andOsPR-pha remained unchanged. Taken together, these resultssuggest that BBTI4 may play a role in rice resistance to Xoo,and OsPR3 and OsPR10a may be involved in the OX-BBTI4-dependent partial Xoo resistance response.
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Baoping Guo,Zhuangzhi Zhang,Xueting Zheng,Yongzhong Guo,Li Zhao,Ren Cai,Bingjie Wang,Mei Yang,Xi Shou,Wenbao Zhang,Bin Jia 대한기생충학ㆍ열대의학회 2019 The Korean Journal of Parasitology Vol.57 No.2
Echinococcus granulosus is an important zoonotic parasite globally causing cystic echinococcosis (CE) in hu- mans and animals. In this study, prevalence of CE and variation of cox1 gene sequence were analyzed with isolates E. granulosus collected from different areas in northern Xinjiang, China. The survey showed that 3.5% of sheep and 4.1% of cattle were infected with CE. Fragment of cox1 was amplified from all the positive sheep and cattle samples by PCR. In addition, 26 positive samples across the 4 areas were included. The isolates were all E. granulosus sensu stricto (s.s.) containing 15 haplotypes (Hap1-15), and clustered into 2 genotypes, G1 (90.1%, 91/101) and G3 (9.9%, 10/101). Hap1 was the most common haplotype (48.5%, 49/101). Hap9 were found in humans samples, indicating that sheep and cattle reservoir human CE. It is indicate that E. granulosus may impact on control of CE in livestock and humans in the region.
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Yang Zhao,Chaoxuan Shang,Zhuangzhi Han,Yuanwei Yin,Ning Han,Hui Xie 한국전자통신연구원 2019 ETRI Journal Vol.41 No.3
Accurate suppressive jamming is a prominent problem faced by radar equipment. It is difficult to solve signal detection problems for extremely low signal to noise ratios using traditional signal processing methods. In this study, a joint sensing dictionary based compressed sensing and adaptive iterative optimization algorithm is proposed to counter suppressive jamming in information domain. Prior information of the linear frequency modulation (LFM) and suppressive jamming signals are fully used by constructing a joint sensing dictionary. The jamming sensing dictionary is further adaptively optimized to perfectly match actual jamming signals. Finally, through the precise reconstruction of the jamming signal, high detection precision of the original LFM signal is realized. The construction of sensing dictionary adopts the Pei type fast fractional Fourier decomposition method, which serves as an efficient basis for the LFM signal. The proposed adaptive iterative optimization algorithm can solve grid mismatch problems brought on by undetermined signals and quickly achieve higher detection precision. The simulation results clearly show the effectiveness of the method.
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Jiaxin Pi,Xu Gao,Yue Yu,Yin Zheng,Zhuangzhi Zhu,Yajing Wang 대한약학회 2021 Archives of Pharmacal Research Vol.44 No.9
Bakuchiol (BAK), isolated from the seeds ofPsoralea corylifolia L., recently presents a variety ofpharmacologic activities. However, the poor oral bioavailabilitylimits its further development and clinical use. The purpose of this study was to establish a self-microemulsifying(SME) formulation for oral deliveryimprovement of BAK. The optimized liquid SME formulationwas comprised of BAK (40 %), Cremophor RH 40(30 %) and Labrasol (30 %). The emulsion droplets werespherical in shape, and particle size and zeta potential weredetermined. The in vitro dissolution test of BAK-SMEformulation illustrated faster dissolution rate than the bulkdrug. The permeabilities of 40 lg mL-1 BAK-SME formulationin rat intestinal segments of duodenum, jejunum,ileum and colon were 30.91 9 10-3, 23.61 9 10-3,29.43 9 10-3 and 23.62 9 10-3 cm min-1, respectively,exhibiting 3.99 times in duodenum, 2.59 times in ileum and 2.31 times in colon greater than BAK perfusate. The oralbioavailability of BAK-SME formulation at a dose of150 mg kg-1 was determined in rats. The Cmax and theAUC(0–24h) were 515.4 ng mL-1 and 4,327.2 h ng mL-1,respectively, which were 1.90 fold and 1.73 fold greaterthan the value of BAK suspension. All these results clearlystated that BAK-SME formulation performed wellimprovementon oral bioavailability of BAK.
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