Characteristics Comparison before and after Lyophilization of Transferrin Modified Procationic-Liposome-Protamine-DNA Complexes (Tf-PLPD)

Zhi-Rong Zhong,Zhi-rong Zhang,Ji Liu,Yong Deng,Hong-wei Zhang,Yao Fu,Qing-guo Song,Qin He 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.1

A novel non-viral gene delivery system, Procationic-Liposome-Protamine-DNA complexes (PLPD) which could further adsorb transferrin on the surface as a targeting ligand to form Tf- PLPD, was prepared and characterized before and after lyophilization. The size distribution of Tf-PLPD was in the range of 240 ± 12 nm and the zeta potential was -24.10 ± 2.5 mV. The transfection efficiencies of PLPD and Tf-PLPD were 12.18 ± 3.8 and 24.26 ± 2.6 mU β-galactosidase/ mg protein respectively. The lyophilization and the presence of serum didn’t affect the tansfectivities of PLPD or Tf-PLPD. Compared to LipofectamineTM 2000 (Invitrogen, U.S.A.), the procationic liposomes had less cytotoxicity to cells. In summary the procationic lipoplex described here, combining the condensing effect of protamine and the targeting capability of Tf, was a perspective non-viral vector for gene delivery system.

An Improved Variable Step Size MPPT Algorithm Based on INC

Zhi-rong Xu,Ping Yang,Dong-bao Zhou,Peng Li,Jin-yong Lei,Yuan-rui Chen 전력전자학회 2015 JOURNAL OF POWER ELECTRONICS Vol.15 No.2

In order to ensure that photovoltaic (PV) systems work at the maximum power point (MPP) and maximize the economic benefits, maximum power point tracking (MPPT) techniques are normally applied to these systems. One of the most widely applied MPPT methods is the incremental conductance (INC) method. However, the choice of the step size still remains controversial. This paper presents an improved variable step size INC MPPT algorithm that uses four different step sizes. This method has the advantages of INC but with the ability to validly adjust the step size to adapt to changes of the PV’s power curve. The presented algorithm also simultaneously achieves increased rapidity and accuracy when compared with the conventional fixed step size INC MPPT algorithm. In addition, the theoretical derivation and specific applications of the proposed algorithm are presented here. This method is validated by simulation and experimental results.

Characteristics Comparison before and after Lyophilization of Transferrin Modified Procationic-Liposome-Protamine-DNA Complexes (Tf-PLPD)

Zhong, Zhi-Rong,Zhang, Zhi-Rong,Liu, Ji,Deng, Yong,Zhang, Hong-Wei,Fu, Yao,Song, Qing-Guo,He, Qin 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.1

A novel non-viral gene delivery system, Procationic-Liposome-Protamine-DNA complexes (PLPD) which could further adsorb transferrin on the surface as a targeting ligand to form Tf-PLPD, was prepared and characterized before and after lyophilization. The size distribution of Tf-PLPD was in the range of $240{\pm}12nm$ and the zeta potential was $-24.10{\pm}2.5mV$. The transfection efficiencies of PLPD and Tf-PLPD were $12.18{\pm}3.8\;and\;24.26{\pm}2.6mU\;{\beta}-galactosidase/mg$ protein respectively. The lyophilization and the presence of serum didn't affect the tansfectivities of PLPD or Tf-PLPD. Compared to $Lipofectamine^{TM}$ 2000 (Invitrogen, U.S.A.), the procationic liposomes had less cytotoxicity to cells. In summary the procationic lipoplex described here, combining the condensing effect of protamine and the targeting capability of Tf, was a perspective non-viral vector for gene delivery system.

Damage identification in beam-like pipeline based on modal information

Yang, Zhi-Rong,Li, Hong-Sheng,Guo, Xing-Lin,Li, Hong-Yan Techno-Press 2007 Structural Engineering and Mechanics, An Int'l Jou Vol.26 No.2

Damage detection based on measured vibration data has received intensive studies recently. Frequently, the damage to a structure may be reflected by a change of some system parameters, such as a degradation of the stiffness. In this paper, we apply a method to nondestructively locate and estimate the severity of damage in corrosion pipeline for which a few natural frequencies or mode shapes are available. The method is based on the strain modal sensitivity ratio (SMSR) and the orthogonality conditions sensitivities (OCS) applied to vibration features identified during the monitoring of the pipeline. The advantage of these methods is that it only requires measuring few modal parameters. The SMSR-based and OCS-based damage detection methods are illustrated using computer-simulated and laboratory testing data. The results show that the current method provides a precise indication of both the location and the extent of corrosion pipeline.

An Improved Variable Step Size MPPT Algorithm Based on INC

Xu, Zhi-Rong,Yang, Ping,Zhou, Dong-Bao,Li, Peng,Lei, Jin-Yong,Chen, Yuan-Rui The Korean Institute of Power Electronics 2015 JOURNAL OF POWER ELECTRONICS Vol.15 No.2

In order to ensure that photovoltaic (PV) systems work at the maximum power point (MPP) and maximize the economic benefits, maximum power point tracking (MPPT) techniques are normally applied to these systems. One of the most widely applied MPPT methods is the incremental conductance (INC) method. However, the choice of the step size still remains controversial. This paper presents an improved variable step size INC MPPT algorithm that uses four different step sizes. This method has the advantages of INC but with the ability to validly adjust the step size to adapt to changes of the PV's power curve. The presented algorithm also simultaneously achieves increased rapidity and accuracy when compared with the conventional fixed step size INC MPPT algorithm. In addition, the theoretical derivation and specific applications of the proposed algorithm are presented here. This method is validated by simulation and experimental results.

Interferon Apha 2b for Treating Patients with JAK2V617F Positive Polycythemia Vera and Essential Thrombocytosis

Zhang, Zhi-Rong,Duan, Yan-Chao Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.4

Objective: To investigate interferon (IFN) alpha 2 b for treating patients with JAK2V617F positive polycythemia vera (PV) and essential thrombocytosis (ET). Methods: Interferon alpha 2 b was used to treat patients with JAK2V617F positive PV and ET. In control group, hydroxyurea was used. Endpoint of study was to compare rates of hematological and molecular remission. Results: Patients in the interferon alpha 2 b group achieved higher rates of hematologic and molecular remission than patients in the hydroxyurea group, with a lower incidence of thrombosis. Conclusion: Compared with hydroxyurea, interferon alpha 2 b could reduce JAK2V617F load for patients with PV and ET, and achieve higher molecular remission, improve treatment efficacy and reduce complications.

Cancer Registration in the Peoples Republic of China

Wei, Kuang-Rong,Chen, Wan-Qing,Zhang, Si-Wei,Liang, Zhi-Heng,Zheng, Rong-Shou,Ou, Zhi-Xiong Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8

The current situation of cancer registration in China was systematically reviewed. So far, cancer registration in China has been making a great progress in the following aspects: the number of cancer registries and covered population have increased dramatically; a registration network has been established and completed gradually; regulations and rules improved remarkably; more attention is being paid by every level of government; a lot of registration software has been created and financial support ensured. However, we are still facing some problems and challenges, such as no stable groups of registrars, shortage of training opportunities, poor data quality, insufficient utilization and lack of multidisciplinary mechanisms, so that the cancer registration system still needs to be enhanced and improved. Along with the development of economy, science and information technology, methods and patterns of cancer registration is changing. It is to be expected that cancer registration will be automatic, nationwide and integrated with community healthcare in the near future.

Transmembrane Helix of Novel Oncogene with Kinase-Domain (NOK) Influences Its Oligomerization and Limits the Activation of RAS/MAPK Signaling

Ying-Hua Li,Yin-Yin Wang,Shan Zhong,Zhi-Li Rong,Yong-Ming Ren,Zhi-Yong Li,Shu-Ping Zhang,Zhi-Jie Chang,Li Liu 한국분자세포생물학회 2009 Molecules and cells Vol.27 No.1

Ligand-dependent or independent oligomerization of receptor protein tyrosine kinase (RPTK) is often an essential step for receptor activation and intracellular signaling. The novel oncogene with kinase-domain (NOK) is a unique RPTK that almost completely lacks an ectodomain, expresses intracellularly and activates constitutively. However, it is unknown whether NOK can form oligomer or what function oligomerization would have. In this study, two NOK deletion mutants were generated by either removing the ectodomain (NOKECD) or including the endodomain (NOK-ICD). Co-immunoprecipitation demonstrated that the transmembrane (TM) domain of NOK was essential for its intermolecular interaction. The results further showed that NOK aggregated more closely as lower order oligomers (the dimer- and trimer-sized) than either deletion mutant did since NOK could be cross-linked by both Sulfo-EGS and formaldehyde, whereas either deletion mutant was only sensitive to Sulfo-EGS. Removing the NOK TM domain (NOK-ICD) not only markedly promoted higher order oligomerization, but also altered the subcellular localization of NOK and dramatically elevated the NOK-mediated constitutive activation of extracellular signal-regulated kinase (ERK). Moreover, NOK-ICD but not NOK or NOKECD was co-localized with the upstream signaling molecule RAS on cell membrane. Thus, TM-mediated intermolecular contacting may be mainly responsible for the constitutive activation of NOK and contribute to the autoinhibitory effect on RAS/MAPK signaling.

Influence of a community of buildings on tornadic wind fields

Zhi Li,Ryan Honerkamp,Gui-rong Yan,Ruoqiang Feng 한국풍공학회 2020 Wind and Structures, An International Journal (WAS Vol.30 No.2

To determine tornadic wind loads, the wind pressure, forces and moments induced by tornadoes on civil structures have been studied. However, in most previous studies, only the individual building of interest was included in the wind field, which may be suitable to simulate the case where a tornado strikes rural areas. The statistical data has indicated that tornadoes induce more significant fatalities and property loss when they attack densely populated areas. To simulate this case, all buildings in the community of interest should be included in the wind field. However, this has been rarely studied. To bridge this research gap, this study will systematically investigate the influence of a community of buildings on tornadic wind fields by modeling all buildings in the community into the wind field (designated as “the Community case under tornadic winds”). For comparison, the case in which only a single building is included in the tornadic wind field (designated as “the Single-building case under tornadic winds”) and the case where a community of buildings are included in the equivalent straight-line wind field (designated as “the Community case under straight-line winds”) are also simulated. The results demonstrate that the presence of a number of buildings completely destroys the pattern of regular circular strips in the distribution of tangential velocity and pressure on horizontal planes. Above the roof height, the maximum tangential velocity is lower in the Community case under tornadic winds than that in the Single-building case under tornadic winds because of the higher surface friction in the Community case; below the roof height, greater tangential velocity and pressure are observed in the Community case under tornadic wind fields, and more unfavorable conditions are observed in the Community case under tornadic winds than under the equivalent straight-line winds.

Incidence of Thymoma in Myasthenia Gravis: A Systematic Review

Zhi-Feng Mao,Xue-An Mo,Yong-Rong Lai,Maree L. Hackett 대한신경과학회 2012 Journal of Clinical Neurology Vol.8 No.3

Background and Purpose Myasthenia gravis (MG) is usually comorbid with thymoma. More accurate estimates of the incidence thymoma in MG will help inform patients and their physicians, facilitate health policy discussions, provide etiologic clues, and optimize the management of MG. Methods We conducted a systematic review search of relevant English-language studies published between 1960 and 2012 using MEDLINE and Embase. We identified additional studies by reviewing the bibliographies of the retrieved articles and hand searched the main neurology journals. Only incidence studies and case series of unselected MG patients in which information about thymoma were included. Results Out of 2206 potentially relevant studies, 49 met the inclusion criteria. Although there was a considerable degree of heterogeneity, the pooled estimate of the incidence of thymoma in MG was 21% (95% confidence interval, 20-22%). The pooled incidence was significantly higher for surgery-based studies than for population- and hospital-based studies. A large proportion of the reported thymomas were noninvasive. Furthermore, thymoma appears to occur significantly more frequently among male MG patients and those older than 40 years at the onset of MG. Conclusions Thymoma is common in MG patients, but appears to be found more often in male MG patients and those older than 40 years at the onset of MG. Further research is needed to expand our understanding of these association conditions