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Mechanical Properties and Damage Characteristics of Coal-Rock Combination with Different Dip Angles
Zhi-Guo Xia,Shuai Liu,Zhuang Bian,Jinhu Song,Fan Feng,Ning Jiang 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.5
The effect of the dip angle of an interlayer coal seam on rock mass mechanical behavior and damage characteristics is seldom discussed. To address the lack of the existing research, a numerical simulation was conducted using the Particle Flow Code (PFC2D), and its damage to mechanical behavior, acoustic emission (AE) characteristics, and damage characteristics were analyzed. The outcomes exhibit the peak strength and elastic modulus of coal-rock combinations are between that of rock and coal. As the coal inclination angle rises, the elastic modulus and peak strength gradually decrease. AE model experiences the initial growth period, stable growth period, and rapid decline period. The value of AE hits and the generation of the AE signal is affected by the size of the inclination angle. The degree of damage to the coal-rock combination gradually rises as the angle rises. The damage process of the coal and rock composite sample encompasses an initial damage phase, damage stable evolution phase, accelerated damage phase, and a failure phase. The damage values of the stable evolution stage for varying dip angle models are also different. As the inclination angle increases, the damage value gradually increases, while the strain at the same damage value gradually decreases.
In Vitro Biological Characterization of DCUN1D5 in DNA Damage Response
Guo, Wei,Li, Guo-Jun,Xu, Hong-Bo,Xie, Jie-Shi,Shi, Tai-Ping,Zhang, Sheng-Zhong,Chen, Xiao-Hong,Huang, Zhi-Gang Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8
Background: Novel prognostic biomarkers or therapeutic molecular targets for laryngeal squamous cell carcinoma (LSCC) are an urgent priority. We here sought to identify multiple novel LSCC-associated genes. Methods: Using high-density microarray expression profiling, we identified multiple genes that were significantly altered between human LSCCs and paired normal tissues. Potential oncogenic functions of one such gene, DCUN1D5, were further characterized in vitro. Results: Our results demonstrated that DCUN1D5 was highly expressed in LSCCs. Overexpression of DCUN1D5 in vitro resulted in 2.7-fold increased cellular migration, 67.5% increased invasive capacity, and 2.6-fold increased proliferation. Endogenous DCUN1D5 expression was decreased in a time-dependent manner after genotoxic stress, and silencing of DCUN1D5 by siRNA decreased the number of cells in the S phase by 10.2% and increased apoptosis by 11.7%. Conclusion: Our data suggest that DCUN1D5 in vitro might have vital roles in DNA damage response, but further studies are warranted to assess its significance in vivo.
Zhi-Fu Guo,Xiang-Yu Long,Pan Dong,Yu-Ming Wei,Li-Ping Bai,Xiao-Xuan Dang,Hao-Lei Wan,Li-Jun Zhang,You-Liang Zheng 한국유전학회 2011 Genes & Genomics Vol.33 No.2
The α-gliadins from Crithopsis delileana (Schult) Roshev (2n=2x=14, KK) were investigated by Acid polyacrylamide gel electrophoresis (A-PAGE) analysis. It was indicated that the electrophoresis mobility of gliadins from C.delileana had obvious difference with those from common wheat in α, γand ω region. Using primers designed from published sequences of α-gliadin genes, three α-gliadin genes were isolated from C. delileana, which were designated as gli-ka1,gli-ka2 and gli-ka3, respectively. Two in-frame stop codons were found in the coding sequences of gli-ka3, indicating that gli-ka3 could be a pseudogene. The gli-ka2 was a gliadin with an odd number of cysteines, resulting from a non-synonymous mutation. This change might lead to the interactive behavior of gli-ka2. Three α-gliadin genes of C. delileana had the similar but not identical primary structures to the corresponding gene sequences from other wheat related species. By the alignment of α-gliadin genes from Triticeae,phylogenetic analysis indicated that three α-gliadin genes of C. delileana clustered together with all α-gliadin genes from Ee genome of Lophopyrum elongatum by an interior paralleled branch.
Molecular cloning of two novel stearoyl-acyl desaturase genes from winterness wheat
Zhi-Fu Guo,Feng-Zhen Li,Xiao-Gang Ma,Feng Lin,Hui Ma,Li-Jing Chen,Ming Zhong,Li-Ping Bai,Ying Yi 한국유전학회 2011 Genes & Genomics Vol.33 No.5
Using RT-PCR and rapid amplification of cDNA ends, two new full-length cDNAs of SAD (TaSAD1 and TaSAD2) were obtained from a hardiest winter wheat cultivar (Mironovskaya808). Sequence comparison analysis showed that the deduced amino acid sequences of TaSAD1 and TaSAD2 had high similarity to those of other reported SAD proteins. They were also different each other by some substitutions, insertions and/or deletions involving single amino acid residues or motifs. Based on evolution analysis, it was clear that all SAD genes from Poaceae were closer than those from other genus such as Arabidopsis,Glycine, Triadica, Brassica, Sesamum and Bassia. All SAD genes clustered into two major groups in Poaceae. Meanwhile,TaSAD1 and TaSAD2 were clustered into different groups. The tertiary structure prediction indicated that both TaSAD1 and TaSAD2 proteins were a compact globular protein and their model structures almost were the same.
A new experimental technique on the tubular joints of spatial grid structures
Zhi-Hua Chen,Guo-Jun Sun,Zhi-Shan Luo 국제구조공학회 2011 Structural Engineering and Mechanics, An Int'l Jou Vol.40 No.6
A new experimental technique was developed on the plate-inserted welded tubular joints of spatial grid structures. In the experimental technique, a microcomputer controlling instrument of moire intervention (MCIMI) was adopted. A test was designed on the plate-inserted welded tubular joints of spatial grid structures to show the effectiveness of the MCIMI technique. Both traditional electrical measuring technique and MCIMI technique were employed in the test. The test results showed that the MCIMI technique was feasible in the case of the complicated tests on steel structures. The MCIMI technique not only implemented the limitation of traditional electrical measuring technique, but also improved the accuracy of the test. According to the test results, we further examined the plate-inserted welded tubular joints in the cable-stayed spatial grids of the Binhai International Convention & Exhibition in Tianjin, China. The analysis showed the joints are safely designed with adequate conservatism. The research provided a new application of MCIMI in the field of large-scale structure engineering.
Zhi-Rong Zhong,Zhi-rong Zhang,Ji Liu,Yong Deng,Hong-wei Zhang,Yao Fu,Qing-guo Song,Qin He 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.1
A novel non-viral gene delivery system, Procationic-Liposome-Protamine-DNA complexes (PLPD) which could further adsorb transferrin on the surface as a targeting ligand to form Tf- PLPD, was prepared and characterized before and after lyophilization. The size distribution of Tf-PLPD was in the range of 240 ± 12 nm and the zeta potential was -24.10 ± 2.5 mV. The transfection efficiencies of PLPD and Tf-PLPD were 12.18 ± 3.8 and 24.26 ± 2.6 mU β-galactosidase/ mg protein respectively. The lyophilization and the presence of serum didn’t affect the tansfectivities of PLPD or Tf-PLPD. Compared to LipofectamineTM 2000 (Invitrogen, U.S.A.), the procationic liposomes had less cytotoxicity to cells. In summary the procationic lipoplex described here, combining the condensing effect of protamine and the targeting capability of Tf, was a perspective non-viral vector for gene delivery system.