The complete mitochondrial genome of a threatened loach (Sinibotia reevesae) and its phylogeny

Yuan‑Chao Zou,Bi‑Wen Xie,Chuan‑Jie Qin,Yong‑Ming Wang,Deng‑Yue Yuan,Rui Li,Zheng‑Yong Wen 한국유전학회 2017 Genes & Genomics Vol.39 No.7

In present study, the complete mitochondrial genome of Sinibotia reevesae was first sequenced using the next-generation sequencing technology and annotated using bioinformatic tools. The circular mitochondrial genome was 16,572 bp in length, and contained 13 proteincoding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, and 1 displacement loop locus. It presents a typical gene organization and order for completely sequenced cypriniformes mitogenomes. The control region could be divided into three parts included the extended termination associated sequence domain, the central conserved domain and the conserved sequence block. Interestingly, two stemloop domains were found in control region and OL region, respectively. Furthermore, phylogenetic analyses using concatenated amino acid and nucleotide sequences of the 13 protein-coding genes with two different methods (Maximum likelihood and Bayesian analysis) both highly supported the close relationship of S. reevesae and Sinibotia superciliaris, which was in line with the previous classifications based on morphological and molecular studies. These data provide useful information for a better understanding of the mitogenomic diversities and evolution in fish as well as novel genetic markers for studying population genetics and species identification.

Removal of elemental mercury using TiO₂-based nanofibers from coal combustion flue gas

( Yuan Yuan ),( Yong Chun Zhao ),( Ji Hua Qiu ),( Ya Qing Qiu ),( Chu Guang Zheng ),( Jun Ying Zhang ) 한국폐기물자원순환학회 2023 I-CIPEC Vol.2012 No.0

Robust Vehicle Registration Method Based on 3D Model for Traffic Surveillance Application

Yuan Zheng,Silong Peng 보안공학연구지원센터(IJSIP) 2013 International Journal of Signal Processing, Image Vol.6 No.5

Baicalin Induces Apoptosis in Leukemia HL-60/ADR Cells via Possible Down-regulation of the PI3K/Akt Signaling Pathway

Zheng, Jing,Hu, Jian-Da,Chen, Ying-Yu,Chen, Bu-Yuan,Huang, Yi,Zheng, Zhi Hong,Liu, Ting-Bo Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4

Background: The effect and possible mechanism of traditional Chinese medicine, baicalin, on the PI3K/Akt signaling pathway in drug-resistant human myeloid leukemia HL-60/ADR cells have been investigated in this current study. Methods: HL-60/ADR cells were treated by 20, 40, $80\;{\mu}mol/L$ baicalin followed by cell cycle analysis at 24h. The mRNA expression level of the apoptosis related gene, Bcl-2 and bad, were measured by RT-PCR on cells treated with $80\;{\mu}mol/L$ baicalin at 12, 24 and 48hr. Western blot was performed to detect the changes in the expression of the proteins related to HL-60/ADR cell apoptosis and the signaling pathway before and after baicalin treatment, including Bcl-2, PARP, Bad, Caspase 3, Akt, p-Akt, NF-${\kappa}B$, p-NF-${\kappa}B$, mTOR and p-mTOR. Results: Sub-G1 peak of HL-60/ADR cells appeared 24 h after $20\;{\mu}mol/L$ baicalin treatment, and the ratio increased as baicalin concentration increased. Cell cycle analysis showed 44.9% G0/G1 phase cells 24 h after baicalin treatment compared to 39.6% in the control group. Cells treated with $80\;{\mu}mol/L$ baicalin displayed a trend in decreasing of Bcl-2 mRNA expression over time. Expression level of the Bcl-2 and PARP proteins decreased significantly while that of the PARP, Caspase-3, and Bad proteins gradually increased. No significant difference in Akt expression was observed between treated and the control groups. However, the expression levels of p-Akt, NF-${\kappa}B$, p-NF-${\kappa}B$, mTOR and p-mTOR decreased significantly in a time-dependent manner. Conclusions: We conclude that baicalin may induce HL-60/ADR cell apoptosis through the PI3K/AKT signaling pathway.

GPIO BASED SLIDING MODE CONTROL FOR DIESEL ENGINE HIGH PRESSURE COMMON RAIL SYSTEM

Zheng Yuan,Chen Dai,Hao Sun,Shihua Li,Bifeng Yin 한국자동차공학회 2023 International journal of automotive technology Vol.24 No.1

With the rapid development of diesel engines, reducing the pressure fluctuation of the common rail has become a key factor to improve the performance of the high pressure common rail (HPCR) system. Among traditional rail pressure control approaches, the influence of time-varying disturbances on the HPCR system is not fully considered and is not well dealt with. To this end, a nonlinear model of the HPCR system is firstly established based on fluid dynamics and mechanics laws. And a composite controller based on the nonlinear model is proposed. It consists of two parts: 1) A sliding mode feedback part; 2) A disturbance feedforward compensation part based on a generalized proportional integral observer. Finally, a group of test is simulated in the AMESim simulation environment and the results are shown to demonstrate the effectiveness of the proposed method.

TIAM2 Enhances Non-small Cell Lung Cancer Cell Invasion and Motility

Zhao, Zheng-Yuan,Han, Chen-Guang,Liu, Jun-Tao,Wang, Chang-Lei,Wang, Yi,Cheng, Li-Ya Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11

Background: TIAM2, a Rac guanine nucleotide exchange factor, is closely associated with cell adherence and migration. Here, we aimed to investigate the role of TIAM2 in non-small cell lung cancer (NSCLC) cells. Materials and Methods: A small interference RNA (siRNA) was introduced to silence the expression of TIAM2. Invasion and motility assays were then performed to assess the invasion and motility potential of NSCLC cells. GST-pull down assays were used to detect activation of Rac1. Results: TIAM2 was highly expressed in NSCLC cells. Knockdown of TIAM2 inhibited the invasion and motility, and suppressed activation of Rac1. Further experiments demonstrated that knockdown of TIAM2 could up-regulate the expression of E-cadherin, and down-regulate the expression of MMP-3, Twist and Snail. Conclusions: Our data suggest that TIAM2 can promote invasion and motility of NSCLC cells. Activation of Rac1 and regulation of some EMT/invasion-related genes may be involved in the underlying processes.

중국 노인 여가스포츠 참가자의 참여동기와 행복에 관한 연구

정원남(Zheng Yuan Nan),홍미화(Hong Mi Hwa),최영래(Choi Young Lae) 글로벌시니어건강증진개발원 2011 글로벌시니어건강증진개발원 논문집 Vol.1 No.1

본 연구는 중국 노인 여가스포츠 참가자의 참여동기와 행복의 관계에 관한 선행연구 결과를 기초로 하여 인구학적 특성에 따른 여가스포츠 참여동기, 행복의 차이를 실증적으로 규명하는데 목적이 있다. 이와 같은 연구목적을 달성하기 위하여 2011년 현재 중국 북경시에 살고 있는 만 65세 이상의 여가스포츠에 참가하고 있는 노인을 모집단으로 선정한 다음 집락무선표집법(Cluster Random Sampling Method)을 이용하여 표본을 500부 추출하였으며, 불성실하게 응답한 자료를 제외하고 총 460명의 자료를 최종분석에 사용하였다. 자료처리는 SPSS Windows 17.0 version을 이용하여 빈도분석, 탐색적 요인분석, 신뢰도분석, T검정, One-way Anova를 실시하였으며, 다음과 같은 결론을 도출하였다. 첫째, 인구학적 특성에 따른 여가스포츠 참여동기 차이에서는 성별은 남자가 여자보다 외적동기가 높게, 연령은 75세 이상 노인들의 65~74세 노인들보다 내적동기가 높게, 배우자 유무에서는 내적동기와 외적동기가 배우자 있다가 없다보다 높게, 용돈정도에서는 외적동기와 내적동기 모두 넉넉함이 많이부족 보다 높게, 주관적 건강에서는 건강한 노인들이 건강하지 않은 노인들보다 내적동기가 높게 나타났다. 둘째, 인구학적 특성에 따른 여가스포츠 행복의 차이에서는 성별은 여자가 남자보다 행복감이 높게, 연령에서는 생활만족이 65~69세가 70세이상 보다 높게, 행복감은 75세 이상이 65~74세 노인들 보다 높게, 교육수준에서는 대졸·고졸·중졸이 초졸 보다 높게, 배우자 유무에서는 생활만족과 행복감 모두 배우자 있다가 없다 보다 높게, 용돈정도는 생활만족과 행복감 모두 넉넉함이 많이 부족보다 높게 나타났다. The purpose of this study is to proven investigate of participation motivation and leisure constraints of Chinese elderly who participating in leisure sport through precede studies. To attain this purpose, we selected the populations who lived in Beijing, China, in 2011 and more than 65 ages, and participating in leisure sport regularly. fter the process, we extract 500 samples by ‘Cluster Random Sampling’ method. onsequently, we used 460 samples data for our study. SPSS Windows 17.0 version was used for statistical analysis. hrough this program, we conducted frequency analysis, exploratory-factorial analysis, reliability analysis, t-test, One-way ANOVA. he result of our study is as in the following. First of all, in part of demographic factors, external motivation of male was higher than that of female. Internal motivation of the upper 75 ages was higher than those of 65 to 74 ages. nternal and external motivations of the elderly who have spouse were higher than that of who don’t have their spouse. In spending money part, internal and external motivations were higher in abundant person than relatively the poor. In self awareness of physical fitness, internal motivation of healthy person was higher than the unhealthy. Second, the degrees of happiness of female were higher than male in accordance with demographic factors (gender). In life satisfaction part, 65 to 69 ages were higher than upper 70 ages. However, the degrees of happiness of upper 75 ages were higher than that of 65 to 74 ages. The persons who have diploma of college, high school, middle school were higher in life satisfaction than who don’t have. As stated above, the elderly who have spouse were higher in life satisfaction and happiness than that of who don’t have their spouse. And lastly, in spending money part, life satisfaction and happiness were higher in abundant person than relatively the poor.

Improvement of Amidase Production by a Newly Isolated Delftia tsuruhatensis ZJB-05174 Through Optimization of Culture Medium

Wang, Yuan-Shan,Xu, Jian-Miao,Zheng, Ren-Chao,Zheng, Yu-Guo,Shen, Yin-Chu The Korean Society for Microbiology and Biotechnol 2008 Journal of microbiology and biotechnology Vol.18 No.12

The R-amidase production by a newly isolated strain of Delftia tsuruhatensis ZJB-05174 was optimized in this paper. Effects of factors such as carbon sources, nitrogen sources, and inducers on amidase production were investigated. The medium composition was optimized using central composite designs and response surface analysis. The optimal medium components for enhanced amidase production were found to be as follows: glucose, 8.23 g/l; yeast extract, 11.59 g/l; 2,2-(R,S)-dimethylcyclopropane carboxamide, 1.76 g/l; NaCl, 1 g/l; ${KH_2}{PO_4}$ 1 g/l; and ${K_2}{HPO_4}$ 1 g/l. A maximum enzyme production of 528.21 U/l was obtained under the optimized conditions, which was 4.7 times higher than that obtained under initial conditions.

Effects of leadership practices on professional learning communities: the mediating role of trust in colleagues

Xin Zheng,Hongbiao Yin,Yuan Liu,Zheng Ke 서울대학교 교육연구소 2016 Asia Pacific Education Review Vol.17 No.3

The building of professional learning communities has been widely recognized as an effective strategy for schools wanting to improve student performance and enhance teachers’ professional capacity. This study explored the relationship between leadership practices and professional learning communities, with a particular focus on the mediating role of trust in colleagues within the context of mainland China. A total of 215 primary school teachers in a province of southwestern China participated in the study. The results showed that leadership practices and trust in colleagues had positive effects on five factors of professional learning communities, namely a shared sense of purpose, collaborative activity, collective focus on student learning, deprivatized practice, and reflective dialog. Trust in colleagues also mediated the influence of leadership practices on professional learning communities. The implications for facilitating professional learning communities in mainland China are discussed here.

중국 무술무형문화유산의 관광산업화에 관한 탐색적 연구

Zhu Yuan Zheng,강순화 한국비교정부학회 2023 한국비교정부학보 Vol.27 No.4

(Purpose) This paper aims to protect martial arts intangible cultural heritage and realize tourism development by utilizing basic theories and methods of tourism management and applying related theories in the fields of economics, business administration, sociology, folklore, and anthropology. (Design/methodology/approach) First, the basic framework of this study was confirmed by defining the concept of intangible cultural heritage tourism, and an analysis was conducted on the development of tourism industrialization of the three major martial arts intangible cultural heritages in China. Second, an analysis was conducted on the development of tourism industrialization of China's three major martial arts intangible cultural heritages, and shamanism and ami-museul also took advantage of their characteristics to seek a model for tourism industrialization development suitable for the situation. (Findings) China's three major martial arts intangible cultural heritage tourism industrialization projects and implementation plans were analyzed, and through this, data to refer to the development of martial arts intangible cultural heritage for tourism industrialization were prepared. In addition, in terms of legislation, funding, protection and collaboration mechanisms, and talent development of the three major martial arts intangible cultural heritages, policies for tourism industrialization of martial arts intangible cultural heritages were proposed. (Research implications or Originality) This paper laid the practical foundation for the protection, rational use, and development of Chinese intangible cultural heritage through the search for the development of the tourism industrialization of martial arts intangible cultural heritage