Anti-CSC Effects in Human Esophageal Squamous Cell Carcinomas and Eca109/9706 Cells Induced by Nanoliposomal Quercetin Alone or Combined with CD 133 Antiserum

Zheng, Nai-Gang,Mo, Sai-Jun,Li, Jin-Ping,Wu, Jing-Lan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.20

CD133 was recently reported to be a cancer stem cell and prognostic marker. Quercetin is considered as a potential chemopreventive agent due to its involvement in suppression of oxidative stress, proliferation and metastasis. In this study, the expression of CD133/CD44 in esophageal carcinomas and Eca109/9706 cells was explored. In immunoflurorescence the locations of $CD133^+$ and multidrug resistance 1 $(MDR1)^+$ in the same E-cancer cells were coincident, mainly in cytomembranes. In esophageal squamous cell carcinomas detected by double/single immunocytochemistry, small $CD133^+$ cells were located in the basal layer of stratified squamous epithelium, determined as CSLC (cancer stem like cells); $CD44^+$ surrounding the cells appeared in diffuse pattern, and the larger $CD44^+$ (hi) cells were mainly located in the prickle cell layer of the epithelium, as progenitor cells. In E-cancer cells exposed to nanoliposomal quercetin (nLQ with cytomembrane permeability), down-regulation of NF-${\kappa}Bp65$, histone deacetylase 1 (HDAC1) and cyclin D1 and up-regulation of caspase-3 were shown by immunoblotting, and attenuated HDAC1 with nuclear translocation and promoted E-cadherin expression were demonstrated by immunocytochemistry. In particular, enhanced E-cadherin expression reflected the reversed epithelial mesenchymal transition (EMT) capacity of nLQ, acting as cancer attenuator/preventive agent. nLQ acting as an HDAC inhibitor induced apoptotic cells detected by TUNEL assay mediated via HDAC-NF-${\kappa}B$ signaling. Apoptotic effects of liposomal quercetin (LQ, with cytomembrane-philia) combined with CD133 antiserum were also detected by CD133 immunocytochemistry combined with TUNEL assay. The combination could induce greater apoptotic effects than nLQ induced alone, suggesting a novel anti-CSC treatment strategy.

Facile Synthesis of Hollow CuO/MWCNT Composites by Infiltration-Reduction-Oxidation Method as High Performance Lithium-ion Battery Anodes

Zheng, Gang,Li, Zhiang,Lu, Jinhua,Zhang, Jinhua,Chen, Long,Yang, Maoping The Korean Electrochemical Society 2020 Journal of electrochemical science and technology Vol.11 No.4

Hollow copper oxide/multi-walled carbon nanotubes (CuO/MWCNT) composites were fabricated via an optimized infiltration-reduction-oxidation method, which is more facile and easy to control. The crystalline structure and morphology were characterized by X-ray diffraction (XRD), and transmission electron microscopy (TEM). The as-prepared CuO/MWCNT composites deliver an initial capacity of 612.3 mAh·g^-1 and with 80% capacity retention (488.2 mAh·g^-1) after 100 cycles at a current rate of 0.2 A·g^-1. The enhanced electrochemical performance is ascribed to the better electrical conductivity of MWCNT, the hollow structure of CuO particles, and the flexible structure of the CuO/MWCNT composites.

Characteristics and prediction methods for tunnel deformations induced by excavations

Zheng, Gang,Du, Yiming,Cheng, Xuesong,Diao, Yu,Deng, Xu,Wang, Fanjun Techno-Press 2017 Geomechanics & engineering Vol.12 No.3

The unloading effect from excavations can cause the deformation of adjacent tunnels, which may seriously influence the operation and safety of those tunnels. However, systematic studies of the deformation characteristics of tunnels located along side excavations are limited, and simplified methods to predict the influence of excavations on tunnels are also rare. In this study, the simulation capability of a finite element method (FEM) considering the small-strain characteristics of soil was verified using a case study. Then, a large number of FEM simulations examining the influence of excavations on adjacent tunnels were conducted. Based on the simulation results, the deformation characteristics of tunnels at different positions and under four deformation modes of the retaining structure were analyzed. The results indicate that the deformation mode of the retaining structure has a significant influence on the deformation of certain tunnels. When the deformation magnitudes of the retaining structures are the same, the influence degree of the excavation on the tunnel increased in this order: from cantilever type to convex type to composite type to kick-in type. In practical projects, the deformation mode of the retaining structure should be optimized according to the tunnel position, and kick-in deformation should be avoided. Furthermore, two methods to predict the influence of excavations on adjacent tunnels are proposed. Design charts, in terms of normalized tunnel deformation contours, can be used to quantitatively estimate the tunnel deformation. The design table of the excavation influence zones can be applied to determine which influence zone the tunnel is located in.

Numerical and experimental study of multi-bench retained excavations

Zheng, Gang,Nie, Dongqing,Diao, Yu,Liu, Jie,Cheng, Xuesong Techno-Press 2017 Geomechanics & engineering Vol.13 No.5

Earth berms are often left in place to support retaining walls or piles in order to eliminate horizontal struts in excavations of soft soil areas. However, if the excavation depth is relatively large, an earth berm-supported retaining system may not be applicable and could be replaced by a multi-bench retaining system. However, studies on multi-bench retaining systems are limited. The goal of this investigation is to study the deformation characteristics, internal forces and interaction mechanisms of the retaining structures in a multi-bench retaining system and the failure modes of this retaining system. Therefore, a series of model tests of a two-bench retaining system was designed and conducted, and corresponding finite difference simulations were developed to back-analyze the model tests and for further analysis. The tests and numerical results show that the distance between the two rows of retaining piles (bench width) and their embedded lengths can significantly influence the relative movement between the piles; this relative movement determines the horizontal stress distribution in the soil between the two rows of piles (i.e., the bench zone) and thus determines the bending moments in the retaining piles. As the bench width increases, the deformations and bending moments in the retaining piles decrease, while the excavation stability increases. If the second retaining piles are longer than a certain length, they will experience a larger bending moment than the first retaining piles and become the primary retaining structure. In addition, for varying bench widths, the slip surface formation differs, and the failure modes of two-bench retained excavations can be divided into three types: integrated failure, interactive failure and disconnected failure.

Aberrant Epigenetic Alteration in Eca9706 Cells Modulated by Nanoliposomal Quercetin Combined with Butyrate Mediated via Epigenetic-NF-κB Signaling

Zheng, Nai-Gang,Wang, Jun-Ling,Yang, Sheng-Li,Wu, Jing-Lan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.11

Since the epigenetic alteration in tumor cells can be reversed by the dietary polyphenol quercetin (Q) or butyrate (B) with chemopreventive activity, suggesting that Q or B can be used for chemopreventive as well as therapeutic agent against tumors. In this study the polyphenol flavonoid quercetin (Q) or sodium butyrate (B) suppressed human esophageal 9706 cancer cell growth in dose dependent manner, and Q combined with B (Q+B) could further inhibit Eca9706 cell proliferation than that induced by Q or B alone, compared with untreated control group (C) in MTT assay. The reverse expressions of global DNMT1, $NF-{\kappa}Bp65$, HDAC1 and Cyclin D1 were down-regulated, while expressions of caspase-3 and $p16INK4{\alpha}$ were up-regulated, compared with the C group in immunoblotting; the down-regulated HDAC1-IR (-immunoreactivity) with nuclear translocation, and up-regulated E-cadherin-IR demonstrated in immunocytochemistry treated by Q or B, and Q+B also displayed further negatively and positively modulated effects compared with C group. The order of methylation specific (MS) PCR of $p16INK4{\alpha}$: C>B/Q>Q+B group, while the order of E-cadherin expression level was contrary, Q+B>Q/B>C group. Thus, Q/B, especially Q+B display reverse effect targeting both altered DNA methylation and histone acetylation, acting as histone deacetylase inhibitor mediated via epigenetic-$NF-{\kappa}B$ cascade signaling.

A Study on CO Detection System of Vehicle Emissions based on QEPAS

Zheng Gang,Qi Yingjie,Li Wei 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.2

In this paper, the technology of QEPAS was used to detect the concentration of carbon monoxide gas. The regulation of DBF laser’s input current frequency and temperature was made first, current modulation frequency being 0 f  f / 2 16420HZ (frequency modulation is half of the resonance frequency of the QTF) and operating temperature being T=23 degrees Celsius. Then enable the output wavelength to be near 1.567 μm and measure CO gas of different concentrations. Through the analysis of the relationship between the second harmonic signals of different concentration CO gas, it is found that between CO concentration and the second harmonic intensity exists good linear relationship and that the system has no independence on the incident light wavelength and has high sensitivity and large dynamic measurement range. The study provides a new way of thinking for other types of trace gas detection and can be applied to the detection of automobile exhaust emissions.

Experimental study on the performance of compensation grouting in structured soil

Zheng, Gang,Zhang, Xiaoshuang,Diao, Yu,Lei, Huayang Techno-Press 2016 Geomechanics & engineering Vol.10 No.3

Most laboratory test research has focused on grouting efficiency in homogeneous reconstituted soft clay. However, the natural sedimentary soils generally behave differently from reconstituted soils due to the effect of soil structure. A series of laboratory grouting tests were conducted to research the effect of soil structure on the performance of compensation grouting. The effects of grouting volume, overlying load and grouting location on the performance of compensation grouting under different soil structures were also studied. Reconstituted soil was altered with added cement to simulate artificial structured soil. The results showed that the final grouting efficiency was positive and significantly increased with the increase of stress ratio within a certain range when grouting in normally consolidated structured clay. However, in the same low yield stress situation, the artificial structured soil had a lower final grouting efficiency than the overconsolidated reconstituted soil. The larger of normalized grouting volume could increase the final grouting efficiency for both reconstituted and artificial structured soils. Whereas, the effect of the overlying load on final grouting efficiencies was unfavourable, and was independent of the stress ratio. As for the layered soil specimens, grouting in the artificial structured soil layer was the most efficient. In addition, the peak grouting pressure was affected by the stress ratio and the overlying load, and it could be predicted with an empirical equation when the overlying load was less than the yield stress. The end time of primary consolidation and the proportion of secondary consolidation settlement varied with the different soil structures, grouting volumes, overlying loads and grouting locations.

Transverse buckling analysis of spatial diamond-shaped pylon cable-stayed bridge based on energy approach

Xing Zheng,Qiao Huang,Qing-gang Zheng,Zhen Li 국제구조공학회 2022 Structural Engineering and Mechanics, An Int'l Jou Vol.83 No.1

The stability of cable-stayed bridges is an important factor considered during design. In recent years, the novel spatial diamond-shaped bridge pylon has shown its advantages in various aspects, including the static response and the stability performance with the development of cable-stayed bridge towards long-span and heavy-load. Based on the energy approach, this paper presents a practical calculation method of the completed state stability of a cable-stayed bridge with two spatial diamond-shaped pylons. In the analysis, the possible transverse buckling of the girder, the top pylon column, and the mid pylon columns are considered simultaneously. The total potential energy of the spatial diamond-shaped pylon cable-stayed bridge is calculated. And based on the principle of stationary potential energy, the transverse buckling coefficients and corresponding buckling modes are obtained. Furthermore, an example is calculated using the design parameters of the Changtai Yangtze River Bridge, a 1176 m cable-stayed bridge under construction in China, to verify the effectiveness and accuracy of the proposed method in practical engineering. The critical loads and the buckling modes derived by the proposed method are in good agreement with the results of the finite element method. Finally, cable-stayed bridges varying pylon and girder stiffness ratios and pylon geometric dimensions are calculated to discuss the applicability and advantages of the proposed method. And a further discussion on the degrees of the polynomial functions when assuming buckling modes are presented.

用未標注語料庫硏究現代漢語語法語義的可能性

정강 ( Gang Zheng ) 한국중문학회 2009 中國文學硏究 Vol.38 No.-

A Clustering Sleep Scheduling Mechanism Based on Sentinel Nodes Monitor for WSN

Zhan-Yang Xu,Song-Gang Zhao,Zheng-Jun Jing 보안공학연구지원센터 2015 International Journal of Smart Home Vol.9 No.1

This paper proposes a clustering sleep scheduling mechanism based on sentinel nodes monitoring for WSN. The mechanism combines the network clustering strategy with the node dormancy strategy, and improves the method of selecting the candidate cluster heads randomly in Energy-Efficient Unequal Clustering (EEUC) algorithm. The conception of sentinel node is introduced based on EEUC, and the neighbor node set of sentinel node will be dormant when the sentinel node’s data change rate is lower than the setting threshold. Simulation results show that this mechanism can effectively balance the energy consumption of the entire network, and significantly extend the network lifetime.