The Protective Effects of Phenolic Constituents from Gastrodia elata on the Cytotoxicity Induced by KCl and Glutamate

Zhan-Bo Huang,Zhe Wu,Fa-Kui Chen,Li-Bo Zou 대한약학회 2006 Archives of Pharmacal Research Vol.29 No.11

Seven phenolic compounds (1-7) were isolated from the tubers of Gastrodia elata. Their structures were elucidated on the basis of MS and NMR spectral data. p-Ethoxymethyl phenyl-O-β- D-glucoside (1) was proved to be a new compound, with N-(p-hydroxybenzyl)-adenosine (7) isolated from this plant for the first time. In this study, the protective effects of the six constituents (1-6) on PC12 cells against the cytotoxicity induced by KCl and glutamate were also investigated. The viability of the PC12 cells was significantly enhanced by pretreatment with the six phenolic constituents.

The Protective Effects of Phenolic Constituents from Gastrodia elata on the Cytotoxicity Induced by KCI and Glutamate

Huang, Zhan-Bo,Wu, Zhe,Chen, Fa-Kui,Zou, Li-Bo The Pharmaceutical Society of Korea 2006 Archives of Pharmacal Research Vol.29 No.11

Seven phenolic compounds (1-7) were isolated from the tubers of Gastrodia elata. Their structures were elucidated on the basis of MS and NMR spectral data. p-Ethoxymethyl phenyl-O-${\beta}$-D-glucoside (1) was proved to be a new compound, with N-(p-hydroxybenzyl)-adenosine (7) isolated from this plant for the first time. In this study, the protective effects of the six constituents (1-6) on PC12 cells against the cytotoxicity induced by KCI and glutamate were also investigated. The viability of the PC12 cells was significantly enhanced by pretreatment with the six phenolic constituents.

Theoretical and experimental study on load-carrying capacity of combined members consisted of inner and sleeved tubes

Bo Hu,Boqing Gao,Shulin Zhan,Cheng Zhang 국제구조공학회 2013 Structural Engineering and Mechanics, An Int'l Jou Vol.45 No.1

Load-carrying capacity of combined members consisted of inner and sleeved tubes subjected to axial compression was investigated in this paper. Considering the initial bending of the inner tube and perfect elasto-plasticity material model, structural behavior of the sleeved member was analyzed by theoretic deduction, which could be divided into three states: the elastic inner tube contacts the outer sleeved tube, only the inner tube becomes plastic and both the inner and outer sleeved tubes become plastic. Curves between axial compressive loads and lateral displacements of the middle sections of the inner tubes were obtained. Then four sleeved members were analyzed through FEM, and the numerical results were consistent with the theoretic formulas. Finally, experiments of full-scale sleeved members were performed. The results obtained from the theoretical analysis were verified against experimental results. The compressive load-lateral displacement curves from the theoretical analysis and the tests are similar and well indicate the point when the inner tube contacts the sleeved tube. Load-carrying capacity of the inner tube can be improved due to the sleeved tube. This paper provides theoretical basis for application of the sleeved members in reinforcement engineering.

Finite Element and Experimental Analysis of Ultrasonic Vibration Milling of High-Volume Fraction SiCp/Al Composites

Zhan-Li Shi,Dao-Hui Xiang,Hao-Ren Feng,Bang-Fu Wu,Zhi-Meng Zhang,Guo-Fu Gao,Bo Zhao 한국정밀공학회 2021 International Journal of Precision Engineering and Vol.22 No.10

SiCp/Al composites have been widely used in many fields due to their excellent mechanical properties. However, the addition of reinforced phase SiC particles makes the overall properties of the composites hard and brittle, which brings great challenges to milling. Ultrasonic vibration-assisted processing technology has great advantages in processing hard and brittle materials. However, the process of rupture of SiC particles cannot be effectively observed during the test processing, and a large number of tests increase the cost of the test. The combination of finite element analysis and experiment was used to study the machining performance of High-volume fraction SiCp/Al composites in longitudinal-torsional ultrasonicassisted milling (LTUAM), and its feasibility was evaluated by comparing with conventional Milling (CM). By analyzing the trajectories of cutting edges in ultrasonic-assisted milling, It was found that ultrasonic frequency determines the time of periodic contact-separation between chisel edge and workpiece, and ultrasonic amplitude determines the maximum distance of contact-separation. Using ABAQUS finite element software, a polygon SiC particles model with a high-volume fraction was established to investigate the SiC particles crushing process under different ultrasonic-assisted milling conditions. The results showed that high-frequency ultrasonic-assisted milling could soften SiCp/Al composites, and the structural integrity of silicon carbide particles could be better maintained under appropriate ultrasonic amplitudes, reducing the probability of fragmentation. The removal mode was mainly plastic removal or crushing into small particles. The surface roughness value and milling force were reduced, improving the surface quality of the processed composite material. The conclusions of the milling test were basically consistent with the simulation results, which prove the correctness and feasibility of the simulation results

Integrated Protection Unit Design for Power Networks

Zhan-feng Fan,Sheng-ming Ge,Z Q Bo,Lin Wang,Feng-quan Zhou,Xing Liu,Guo-bing Song 보안공학연구지원센터 2016 International Journal of Grid and Distributed Comp Vol.9 No.4

This paper presents an hardware design solution for integrated protection of distribution systems (Network Protection Unit) by combining transient polarity comparison technique, which is based on the detection and processing of fault generated transient current signals. The integrated protection relays installed at each substation of a distribution network are communicated with the Network Protection Unit through specially designed Packet Transport Network (PTN) for fast and reliable transmission of transient polarity current signals. The relay detects the faulted generated super-imposed current signals. The transient polarity identification algorithm is then applied to the super-imposed signals to identify the polarity of the signal detected. The Network Protection Unit can collect all the transient polarity current signals under its protection area. Then The direction of a fault is determined by comparison of the polarity of the signals derived from all the line sections connected to the substation. The actual faulted section is identified by the Network Protection Unit through comparing the directional information from various stations. Simulation results presented in the paper demonstrate the feasibility of the scheme.

Glulam Beam-to-Column Connection with Coupled Steel Rods with Screwheads: Concept and Experimental Verifications

Zhan Shu,Bo Ning,Hanlin Dong,Zheng Li,Zengxin Peng,Yiyu Yang 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.6

The bolted connections with slotted-in steel plates are basic beam-to-column connections of modern post-and-beam timber building structures. However, due to significantly pinched rotational behaviors, the moment resistance of such connections are usually ignored in design. In this study, the connection-level moment resistance is enabled for modern glulam frame structures with an innovative connection design. A pair of long steel rods with screwheads is designed to generate a force couple at the sides of the connection. The proposed connection could be considered as the key lateral force resisting component, indicating a significant reduction of lateral braces and shear walls. Specimens of the target connection were carefully designed and tested in the lab to obtain their mechanical properties and failure modes. Finite element models were established and calibrated according to the tested data. The moment-rotation curves of the new connection could be easily estimated through a fitted formula. Moreover, the suggestions to improve the proposed connection is also discussed based on the observed damage modes. Results show that the new connection could be treated as rigid moment-resisting connections while the ductility could be further improved.

Theoretical and experimental study on load-carrying capacity of combined members consisted of inner and sleeved tubes

Hu, Bo,Gao, Boqing,Zhan, Shulin,Zhang, Cheng Techno-Press 2013 Structural Engineering and Mechanics, An Int'l Jou Vol.45 No.1

Load-carrying capacity of combined members consisted of inner and sleeved tubes subjected to axial compression was investigated in this paper. Considering the initial bending of the inner tube and perfect elasto-plasticity material model, structural behavior of the sleeved member was analyzed by theoretic deduction, which could be divided into three states: the elastic inner tube contacts the outer sleeved tube, only the inner tube becomes plastic and both the inner and outer sleeved tubes become plastic. Curves between axial compressive loads and lateral displacements of the middle sections of the inner tubes were obtained. Then four sleeved members were analyzed through FEM, and the numerical results were consistent with the theoretic formulas. Finally, experiments of full-scale sleeved members were performed. The results obtained from the theoretical analysis were verified against experimental results. The compressive load-lateral displacement curves from the theoretical analysis and the tests are similar and well indicate the point when the inner tube contacts the sleeved tube. Load-carrying capacity of the inner tube can be improved due to the sleeved tube. This paper provides theoretical basis for application of the sleeved members in reinforcement engineering.

Loss of KDM5B ameliorates pathological cardiac fibrosis and dysfunction by epigenetically enhancing ATF3 expression

Wang Bo,Tan Yong,Zhang Yunkai,Zhang Sheng,Duan Xuewen,Jiang Yuyu,Li Tong,Zhou Qingqing,Liu Xingguang,Zhan Zhenzhen 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Excessive cardiac fibrosis is central to adverse cardiac remodeling and dysfunction leading to heart failure in many cardiac diseases. Histone methylation plays a crucial role in various pathophysiological events. However, the role of histone methylation modification enzymes in pathological cardiac fibrosis needs to be fully elucidated. Here, we identified lysine demethylase 5B (KDM5B), a histone H3K4me2/me3 demethylase, as a key epigenetic mediator of pathological cardiac fibrosis. KDM5B expression was upregulated in cardiac fibroblasts and myocardial tissues in response to pathological stress. KDM5B deficiency markedly ameliorated cardiac fibrosis, improved cardiac function, and prevented adverse cardiac remodeling following myocardial infarction (MI) or pressure overload. KDM5B knockout or inhibitor treatment constrained the transition of cardiac fibroblasts to profibrogenic myofibroblasts and suppressed fibrotic responses. KDM5B deficiency also facilitated the transformation of cardiac fibroblasts to endothelial-like cells and promoted angiogenesis in response to myocardial injury. Mechanistically, KDM5B bound to the promoter of activating transcription factor 3 (Atf3), an antifibrotic regulator of cardiac fibrosis, and inhibited ATF3 expression by demethylating the activated H3K4me2/3 modification, leading to the enhanced activation of TGF-β signaling and excessive expression of profibrotic genes. Our study indicates that KDM5B drives pathological cardiac fibrosis and represents a candidate target for intervention in cardiac dysfunction and heart failure.

Proteomic Analysis of Erythritol-Producing Yarrowia lipolytica from Glycerol in Response to Osmotic Pressure(s)

( Li Bo Yang ),( Xiao Meng Dai ),( Zhi Yong Zheng ),( Li Zhu ),( Xiao Bei Zhan ),( Chi Chung Lin ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.7

Osmotic pressure is a critical factor for erythritol production with osmophilic yeast. Protein expression patterns of an erythritol-producing yeast, Yarrowia lipolytica, were analyzed toidentify differentially-expressed proteins in response to osmotic pressure. In order to analyze intracellular protein levels quantitatively, two-dimensional gel electrophoresis was performed to separate and visualize the differential expression of the intracellular proteins extracted from Y. lipolytica cultured under low (3.17 osmol/kg) and high (4.21 osmol/kg) osmotic pressures. Proteomic analyses allowed identification of 54 differentially-expressed proteins among the proteins distributed in the range of pI 3-10 and 14.4-97.4 kDa molecular mass between the osmotic stress conditions. Remarkably, the main proteins were involved in the pathway of energy, metabolism, cell rescue, and stress response. The expression of such enzymes related to protein and nucleotide biosynthesis was inhibited drastically, reflecting the growth arrest of Y. lipolytica under hyperosmotic stress. The improvement of erythritol production under highosmotic stress was due to the significant induction of a range of crucial enzymes related to polyols biosynthesis, such as transketolase and triosephosphate isomerase, and the osmotic stress responsive proteins like pyridoxine-4-dehydrogenase and the AKRs family. The polyols biosynthesis was really related to an osmotic response and a protection mechanism against hyperosmotic stress in Y. lipolytica. Additionally, the high osmotic stress could also induce other cell stress responses as with heat shock and oxidation stress responses, and these responsive proteins, such as the HSPs family, catalase T, and superoxide dismutase, also had drastically increased expression levels under hyperosmotic pressure.

AN EXPLORATIVE STUDY OF PSYCHOLOGICAL FACTORS IMPACTING LITTERING BEHAVIORS

Anran Zhan,Bo Pang,Jeawon Kim,Tuyet-Mai Nguyen,Phong Tuan Nham 글로벌지식마케팅경영학회 2020 Global Marketing Conference Vol.2020 No.11