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        • SCIESCOPUSKCI등재

          Effects of alfalfa flavonoids on the production performance, immune system, and ruminal fermentation of dairy cows

          Zhan,,Jinshun,Liu,,Mingmei,Su,,Xiaoshuang,Zhan,,Kang,Zhang,,Chungang,Zhao,,Guoqi Asian Australasian Association of Animal Productio 2017 Animal Bioscience Vol.30 No.10

          Objective: The objective of this study was to examine the effects of alfalfa flavonoids on the production performance, immunity, and ruminal fermentation of dairy cows. Methods: The experiments employed four primiparous Holstein cows fitted with ruminal cannulas, and used a $4{\times}4$ Latin square design. Cattle were fed total mixed ration supplemented with 0 (control group, Con), 20, 60, or 100 mg of alfalfa flavonoids extract (AFE) per kg of dairy cow body weight (BW). Results: The feed intake of the group receiving 60 mg/kg BW of AFE were significantly higher (p<0.05) than that of the group receiving 100 mg/kg BW. Milk yields and the fat, protein and lactose of milk were unaffected by AFE, while the total solids content of milk reduced (p = 0.05) linearly as AFE supplementation was increased. The somatic cell count of milk in group receiving 60 mg/kg BW of AFE was significantly lower (p<0.05) than that of the control group. Apparent total-tract digestibility of neutral detergent fiber and crude protein showed a tendency to increase (0.05<$p{\leq}0.10$) with ingestion of AFE. Methane dicarboxylic aldehyde concentration decreased (p = 0.03) linearly, whereas superoxide dismutase activity showed a tendency to increase (p = 0.10) quadratically, with increasing levels of AFE supplementation. The lymphocyte count and the proportion of lymphocytes decreased (p = 0.03) linearly, whereas the proportion of neutrophil granulocytes increased (p = 0.01) linearly with increasing levels of dietary AFE supplementation. The valeric acid/total volatile fatty acid (TVFA) ratio was increased (p = 0.01) linearly with increasing of the level of AFE supplementation, the other ruminal fermentation parameters were not affected by AFE supplementation. Relative levels of the rumen microbe Ruminococcus flavefaciens tended to decrease (p = 0.09) quadratically, whereas those of Butyrivibrio fibrisolvens showed a tendency to increase (p = 0.07) quadratically in response to AFE supplementation. Conclusion: The results of this study demonstrate that AFE supplementation can alter composition of milk, and may also have an increase tendency of nutrient digestion by regulating populations of microbes in the rumen, improve antioxidant properties by increasing antioxidant enzyme activities, and affect immunity by altering the proportions of lymphocyte and neutrophil granulocytes in dairy cows. The addition of 60 mg/kg BW of AFE to the diet of dairy cows was shown to be beneficial in this study.

        • SCIESCOPUSKCI등재

          Effects of alfalfa flavonoids extract on the microbial flora of dairy cow rumen

          Zhan,,Jinshun,Liu,,Mingmei,Wu,,Caixia,Su,,Xiaoshuang,Zhan,,Kang,Zhao,,Guo,qi Asian Australasian Association of Animal Productio 2017 Animal Bioscience Vol.30 No.9

          Objective: The effect of flavonoids from alfalfa on the microbial flora was determined using molecular techniques of 16S ribosome deoxyribonucleic acid (rDNA) analysis. Methods: Four primiparous Holstein heifers fitted with ruminal cannulas were used in a $4{\times}4$ Latin square design and fed a total mixed ration to which alfalfa flavonoids extract (AFE) was added at the rates of 0 (A, control), 20 (B), 60 (C), or 100 (D) mg per kg of heifer BW. Results: The number of operational taxonomic units in heifers given higher levels of flavonoid extract (C and D) was higher than for the two other treatments. The Shannon, Ace, and Chao indices for treatment C were significantly higher than for the other treatments (p<0.05). The number of phyla and genera increased linearly with increasing dietary supplementation of AFE (p<0.05). The principal co-ordinates analysis plot showed substantial differences in the microbial flora for the four treatments. The microbial flora in treatment A was similar to that in B, C, and D were similar by the weighted analysis. The richness of Tenericutes at the phylum level tended to increase with increasing AFE (p = 0.10). The proportion of Euryarchaeota at the phylum level increased linearly, whereas the proportion of Fusobacteria decreased linearly with increasing AFE supplementation (p = 0.04). The percentage of Mogibacterium, Pyramidobacter, and Asteroleplasma at the genus level decreased linearly with increasing AFE (p<0.05). The abundance of Spirochaeta, Succinivibrio, and Suttonella at the genus level tended to decrease linearly with increasing AFE (0.05<p<0.10). Conclusion: Including AFE in the diet of dairy cows may alter the microbial composition of the rumen; however its effect on nutrient digestibility remains to be determined.

        • KCI등재SCOPUS

          Effects of alfalfa flavonoids extract on the microbial flora of dairy cow rumen

          Jinshun,Zhan,Mingmei,Liu,Caixia,Wu,Xiaoshuang,Su,Kang,Zhan,Guo,qi,Zhao 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.9

          Objective: The effect of flavonoids from alfalfa on the microbial flora was determined using molecular techniques of 16S ribosome deoxyribonucleic acid (rDNA) analysis. Methods: Four primiparous Holstein heifers fitted with ruminal cannulas were used in a 4×4 Latin square design and fed a total mixed ration to which alfalfa flavonoids extract (AFE) was added at the rates of 0 (A, control), 20 (B), 60 (C), or 100 (D) mg per kg of heifer BW. Results: The number of operational taxonomic units in heifers given higher levels of flavonoid extract (C and D) was higher than for the two other treatments. The Shannon, Ace, and Chao indices for treatment C were significantly higher than for the other treatments (p<0.05). The number of phyla and genera increased linearly with increasing dietary supplementation of AFE (p<0.05). The principal co-ordinates analysis plot showed substantial differences in the microbial flora for the four treatments. The microbial flora in treatment A was similar to that in B, C, and D were similar by the weighted analysis. The richness of Tenericutes at the phylum level tended to increase with increasing AFE (p = 0.10). The proportion of Euryarchaeota at the phylum level increased linearly, whereas the proportion of Fusobacteria decreased linearly with increasing AFE supplementation (p = 0.04). The percentage of Mogibacterium, Pyramidobacter, and Asteroleplasma at the genus level decreased linearly with increasing AFE (p<0.05). The abundance of Spirochaeta, Succinivibrio, and Suttonella at the genus level tended to decrease linearly with increasing AFE (0.05<p<0.10). Conclusion: Including AFE in the diet of dairy cows may alter the microbial composition of the rumen; however its effect on nutrient digestibility remains to be determined.

        • A Novel Brain Tumor Segmentation Method for Multi-Modality Human Brain MRIs

          Tianming,Zhan,Shenghua,Gu,Lei,Jiang,Yongzhao,Zhan 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.11

          Delineating brain tumor boundaries from multi-modality magnetic resonance images (MRIs) is a crucial step in brain cancer surgical and treatment planning. In this paper, we propose a fully automatic technique for brain tumor segmentation from multi-modality human brain MRIs. We first use the intensities of different modalities in MRIs to represent the features of both normal and abnormal tissues. Then, the multiple classifier system (MCS) is applied to calculate the probabilities of brain tumor and normal brain tissue in the whole image. At last, the spatial-contextual information is proposed by constraining the classified neighbors to improve the classification accuracy. Our method was evaluated on 20 multi-modality patient datasets with competitive segmentation results.

        • Brain Tumor Segmentation from Multispectral MRIs Using Sparse Representation Classification and Markov Random Field Regularization

          Tianming,Zhan,Shenghua,Gu,Can,Feng,Yongzhao,Zhan,Jin,Wang 보안공학연구지원센터 2015 International Journal of Signal Processing, Image Vol.8 No.9

          Automatic brain tumor segmentation from multispectral magnetic resonance imaging (MRI) data is an important but a challenging task because of the high diversity in the appearance of tumor tissues among different patients and in many cases similarity with the normal tissues. In this paper, we propose a fully automatic technique for brain tumor segmentation from multispectral human brain MRIs. We first use the intensities of different patches in multispectral MRIs to represent the features of both normal and abnormal tissues and generate a dictionary for following tissue classification. Then, the sparse representation classification (SRC) is applied to classify the brain tumor and normal brain tissue in the whole image. At last, the Markov random field (MRF) regularization introduces spatial constraints to the SRC to take into account the pair-wise homogeneity in terms of classification labels and multispectral voxel intensities. Our method was evaluated on 20 multi-modality patient datasets with competitive segmentation results.

        • KCI등재SCOPUS

          Effects of alfalfa flavonoids on the production performance, immune system, and ruminal fermentation of dairy cows

          Jinshun,Zhan,Mingmei,Liu,Xiaoshuang,Su,Kang,Zhan,Chungang,Zhang,Guo,qi,Zhao 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.10

          Objective: The objective of this study was to examine the effects of alfalfa flavonoids on the production performance, immunity, and ruminal fermentation of dairy cows. Methods: The experiments employed four primiparous Holstein cows fitted with ruminal cannulas, and used a 4×4 Latin square design. Cattle were fed total mixed ration supplemented with 0 (control group, Con), 20, 60, or 100 mg of alfalfa flavonoids extract (AFE) per kg of dairy cow body weight (BW). Results: The feed intake of the group receiving 60 mg/kg BW of AFE were significantly higher (p<0.05) than that of the group receiving 100 mg/kg BW. Milk yields and the fat, protein and lactose of milk were unaffected by AFE, while the total solids content of milk reduced (p = 0.05) linearly as AFE supplementation was increased. The somatic cell count of milk in group receiving 60 mg/kg BW of AFE was significantly lower (p<0.05) than that of the control group. Apparent total-tract digestibility of neutral detergent fiber and crude protein showed a tendency to increase (0.05<p≤0.10) with ingestion of AFE. Methane dicarboxylic aldehyde concentration decreased (p = 0.03) linearly, whereas superoxide dismutase activity showed a tendency to increase (p = 0.10) quadratically, with increasing levels of AFE supplementation. The lymphocyte count and the proportion of lymphocytes decreased (p = 0.03) linearly, whereas the proportion of neutrophil granulocytes increased (p = 0.01) linearly with increasing levels of dietary AFE supplementation. The valeric acid/total volatile fatty acid (TVFA) ratio was increased (p = 0.01) linearly with increasing of the level of AFE supplementation, the other ruminal fermentation parameters were not affected by AFE supplementation. Relative levels of the rumen microbe Ruminococcus flavefaciens tended to decrease (p = 0.09) quadratically, whereas those of Butyrivibrio fibrisolvens showed a tendency to increase (p = 0.07) quadratically in response to AFE supplementation. Conclusion: The results of this study demonstrate that AFE supplementation can alter composition of milk, and may also have an increase tendency of nutrient digestion by regulating populations of microbes in the rumen, improve antioxidant properties by increasing antioxidant enzyme activities, and affect immunity by altering the proportions of lymphocyte and neutrophil granulocytes in dairy cows. The addition of 60 mg/kg BW of AFE to the diet of dairy cows was shown to be beneficial in this study.

        • KCI등재SCOPUS

          Performance of Hepatitis B Core-Related Antigen Versus Hepatitis B Surface Antigen and Hepatitis B Virus DNA in Predicting HBeAg-positive and HBeAg-negative Chronic Hepatitis

          Zhan-qing,Zhang,Yan-bing,Wang,,Wei,Lu,,Dan-ping,Liu,,Bi-sheng,Shi,,Xiao-nan,Zhang,,Dan,Huang,,Xiu-fen,Li,,Xin-lan,Zhou,,Rong-rong,Ding, 대한진단검사의학회 2019 Annals of Laboratory Medicine Vol.39 No.1

          Background: We examined changes in hepatitis B core-related antigen (HBcrAg) during the four sequential phases of chronic hepatitis B virus (HBV) infection: hepatitis B e antigen (HBeAg)-positive chronic infection (EPCI) and hepatitis (EPCH), followed by HBeAg-negative chronic infection (ENCI) and hepatitis (ENCH). We compared the performance of serum HBcrAg, hepatitis B surface antigen (HBsAg), and HBV DNA in predicting EPCH and ENCH.

        • KCI등재SCOPUS

          Finite element model updating of jointed structure based on modal and strain frequency response function

          Zhan,Ming,Guo,Qintao,Yue,Lin,Zhang,Baoqiang 대한기계학회 2019 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.33 No.10

          To acquire a reasonable model for structural dynamic strength analysis, a bottom-up finite element modeling and updating methodology based on multi responses is proposed. The fundamental principles of structural dynamics analysis and model updating were introduced, and the proposed strategy was applied to the case study of an L-shaped jointed structure. Components of the jointed structure were modeled sequentially, and inaccurate model parameters were updated based on the corresponding experimental modal results in the first stage. In the second stage, components were connected together by bolts. The joint interfaces were represented by thin-layer elements, and local joint parameters were updated based on strain frequency response function (FRF). Finally, the precision of finite element model (FEM) was validated by acceleration frequency response function. The results indicated that the proposed methodology is able to reduce model simulation errors in both components and the overall jointed structure. Not only can the updated model of a jointed structure reproduce the experimental results used in updating, but also predict responses that are not used in the process of model updating.

        • KCI등재

          論文 : 何處休閑:資本和權力下的都市 休閑空間分析

          ( Zhan Biao Chen ) 국민대학교 중국지식네트워크 2014 중국지식네트워크 Vol.4 No.-

          當代城市普遍存在著休閑空間궤乏的問題, 造成這種現象主要與以下三個因素相關: (1) 休閑空間爲“城市化”所“抹除”;(2) 休閑空間爲“特權者”所“圈占”;(3) 休閑空間爲“公家人”所“出賣”。正是在“唯利是圖的資本”和“損公肥私的權力”的雙重夾擊下, 城市休閑空間被제兌、被獨占、被出賣。要改變這種情形, 恢復城市必要的休閑空間, 壹方面要放棄“利益最大化”的“算盤思維”, 壹方面要破除“權爲我所用, 利爲我所謀”的“權力意識”。 The contemporary city exists the problem of lack of leisure space. There are three factors accounting for this phenomenon:(1) The leisure space is erased by the urbanization; (2) The leisure space is occupied by the privilege people; (3)The leisure space is sold by the ones who live on the public finance. Under the attacking of the capital and power, city leisure space is cut ,occupied, and sold. To change this situation and restore the city leisure space necessary, one should abandon the abacus thinking(profit maximization) and the power consciousness(seeking profit for me by privilege).

        • KCI등재

          Significant Differences in the Clinicopathological Characteristics and Survival of Gastric Cancer Patients from Two Cancer Centers in China and Korea

          Zhan,Long,Shen,송교영,Ying,Jiang,Ye,Qi,Wei,Xie,Bin,Liang,Kewei,Jiang,박조현,Shan,Wang 대한위암학회 2015 Journal of gastric cancer Vol.15 No.1

          Purpose: To compare the clinicopathological data and long-term survival of gastric cancer patients in China and Korea. Materials and Methods: Patients who had undergone gastrectomy for gastric cancer between 1998 and 2009 in 2 high-volume institutions in both China (n=1,637) and Korea (n=2,231) were retrospectively evaluated. Clinicopathological variables, overall survival (OS), progression-free survival (PFS), and surgery-related complications were assessed for all patients and compared between the 2 institutions. Results: Chinese patients included in the study were significantly older and had a significantly lower body mass index (BMI) than the Korean patients. Esophagogastric junction tumors were more frequent in Chinese patients. However, the number of patients with stage I gastric cancer, the number of harvested lymph nodes, and the number of total gastrectomies were significantly higher in the Korean population. Korean patients also presented with fewer undifferentiated tumors than Chinese patients. Furthermore, Korean patients had prolonged OS and PFS for stage III cancers only. BMI, tumor-node-metastasis (TNM) stage, tumor invasion, number of positive lymph nodes, and distant metastases were all independent factors affecting OS and PFS. Conclusions: Although China and Korea are neighboring Asian countries, the clinicopathological characteristics of Chinese patients are significantly different from those of Korean patients. Korean gastric cancer patients had longer OS and PFS than Chinese patients. Influencing factors included TNM stage, tumor invasion, and lymph node metastasis.

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