Excessive retinoic acid inhibit mouse embryonic palate mesenchymal cell growth through involvement of Smad signaling

Zengli Yu,Xiaozhuan Liu,Zhan Gao,Zhitao Li,Jun Yin,Yuchang Tao,Lingling Cui,Zengli Yu 한국통합생물학회 2017 Animal cells and systems Vol.21 No.1

All-trans retinoic acid (atRA), the oxidative metabolite of retinoic acid (RA), is essential for palatogenesis. Overdose RA is capable of inducing cleft palate in mice and humans. Normal embryonic palatal mesenchymal (EPM) cell growth is crucial for shelf growth. Smad signaling is involved in many biological processes. However, it is not much clear if atRA could affect Smad signaling during EPM cells growth. In this study, the timed pregnant mice with maternal administration of 100 mg/kg body weight of RA by gastric intubation were cervical dislocation executed to evaluate growth changes of palatal shelves by hematoxylin and eosin (H&E) staining. At the same time, a primary mouse EPM (MEPM) cell culture model was also established. MEPM cells were treated with atRA (0.1, 0.5, 1, 5 and 10 μM) for 24, 48 and 72 h. The results indicated that the sizes of the shelves were smaller than those in control. AtRA inhibited MEPM cell growth with both increasing concentration and increasing incubation time, especially at 72 h in vitro. Moreover, atRA significantly increased the mRNA and protein expression levels of Smad7 (P < .05), but the mRNA and protein expression levels of PCNA were reduced (P < .05). We also found atRA inhibited phosphorylation of Smad2 compared with untreated group (P < .05). However, the protein and mRNA levels of Smad2 did not change both in atRA-treated and untreated group (P > .05). We demonstrated that RA induced inhibition of MEPM cell growth that could cause cleft palate partly by down-regulation of Smad pathway.

The Influence of Composite Wave Field Fluctuation Parameters Matching to Permeability of Low Permeability Reservoirs

Zengli Xiao,Junbin Chen,Xiangqian Du,Wenlong Qin 보안공학연구지원센터 2016 International Journal of Smart Home Vol.10 No.7

There are essential differences in the influence rules of the binary composite wave generated by the combination of artificial resonance wave and hydraulic impulse wave to two-phase fluid flow of low permeability reservoirs porous media oil-water. When the fluctuation parameters of binary composite wave field reaching the optimal matching, the binary composite wave will have a good synergetic effect on two-phase fluid flow of oil-water, and the improvement effect on two-phase fluid flow of low permeability reservoirs oil-water will generate resonance effect which is far more effective than unary wave field. If the fluctuation parameters of binary composite wave field are not matched well, bad synergetic effect on two-phase fluid flow of oil-water will be generated ,not only it can’t raise the improvement effect on two-phase fluid flow of oil-water, but also it will make the improvement effect below unary wave field. Even there will be an inhibition effect on fluid flow of oil-water, which reduces the displacement efficiency and the availability of low permeability reservoirs water injection’s development. By using the indoor fluctuation oil recovery simulation experiment device, the influence rules of displacement vibration wave and hydraulic impulse binary composite wave field to low permeability reservoirs oil-water single phase and two-phase fluid flow feature were studied. The influence rules of amplitude, frequency, work cycle and the other important parameters of composite wave to the permeability of oil-water single phase was intensively studied. Finally the mechanism of the synergetic effect generated by composite wave to two-phase fluid flow of low permeability reservoirs was analyzed.

A Electrochemical Sensor based on Poly (Sulfosalicylic Acid) Film Modified Electrode and Application to Phenol Detection in Oilfield Wastewater

Zengli Xiao,Wenlong Qin,Liangliang Shi 보안공학연구지원센터 2016 International Journal of Smart Home Vol.10 No.6

In this paper, a highly sensitive and selective method based on the poly (sulfosalicylic acid) modified electrode (PSA /CPE) to detect phenol was established. The morphologies and interface properties of PSA film were characterized by scanning electron microscopy and electrochemical impedance spectroscopy. It was illustrated that the PSA/CPE had an excellent electrocatalytic ability towards the oxidation of phenol. Meanwhile the influence of parameters such as pH and scan rate effect on the analytical performance of the sensor was evaluated. Moreover, the interference from o-nitrophenol can be neglected. By using differential pulse voltammetry (DPV), linear calibration curves were obtained as 5–175 and 220–555 μmol L−1 for phenol. The detection limits are 2.2 μmol L−1 for phenol. With favorable selectivity and sensitivity, the present method has been applied to the determination of phenol in oilfield wastewater.

Screening of Methylation Gene Sites as Prognostic Signature in Lung Adenocarcinoma

Min Dong,Zengli Yang,Xingfang Li,Zhenxiang Zhang,Ankang Yin 연세대학교의과대학 2020 Yonsei medical journal Vol.61 No.12

Purpose: Most lung adenocarcinoma (LUAD) patients are diagnosed at the advanced stage and have poor prognosis. DNA methylationplays an important role in the prognosis prediction of cancers. The objective of this study was to identify new DNA methylationsites as biomarkers for LUAD prognosis. Materials and Methods: We downloaded DNA methylation data from The Cancer Genome Atlas data portal. Cox proportionalhazard regression model and random survival forest algorithm were applied to identify the DNA-methylation sites. Methylationof sites were validated in the Gene Expression Omnibus cohorts. Function annotation were done to explore the biological functionof DNA methylated sites signature. Results: Six DNA methylation sites were identified as prognosis signature. The signature yielded acceptable discrimination betweenthe high-risk group and low-risk group. The discrimination effect of this DNA methylation signature for the OS was obvious,with a median OS of 21.89 months vs. 17.74 months for high-risk vs. low-risk groups. This prognostic prediction model wasvalidated by the test group and GEO dataset. The predictive survival value was higher for the prognostic prediction model thanthat for the tumor node metastasis stage. Adjuvant hemotherapy could not affect the prediction of the signature. Functional analysisindicated that these signature genes were involved in protein binding and cytoplasm. Conclusion: We identified the prognostic signature for LUAD by combining six DNA methylation sites. This could service as potentialrobust and specificity signature in the prognosis prediction of LUAD.

Study on the measurement accuracy of circular transmission line model for low-resistance Ohmic contacts on III-V wide band-gap semiconductors

Tong Liu,Rong Huang,Fangsen Li,Zengli Huang,Jian Zhang,Jianping Liu,Liqun Zhang,Shuming Zhang,An Dingsun,Hui Yang 한국물리학회 2018 Current Applied Physics Vol.18 No.7

The accuracy and error propagation for determining the low specific contact resistance of Ohmic contacts on IIIV wide band-gap semiconductors based on the circular transmission line model have been analyzed and the validity of this method is discussed in detail. The accuracy is more susceptible to the factors including data fitting method, electrical measurement technique and contact area correction. By using the equations of the original circular transmission line model to extract the fitting parameters, the calculation accuracy is much improved and the inapplicability of the linear least-square fitting is prevented. To further improve the accuracy, a four-probe current-voltage measurement technique was adopted to reduce the parasitic series resistances and the uncertainty bound, especially for the Ohmic contact with low sheet resistance of the semiconductor. Moreover, we have studied the size effect of contact pads of patterns and demonstrated that contact area correction is necessary for the semiconductor with high sheet resistance. A comprehensive error analysis is also performed to fully understand all the impact factors on this advanced method of specific contact resistance measurement, which is benefit for device performance evaluation and failure analysis.

Perovskite-type LaFe1− xMnxO3 (x=0, 0.3, 0.5, 0.7, 1.0) oxygen carriers for chemical-looping steam methane reforming: Oxidation activity and resistance to carbon formation

Kun Zhao,Fang He,Zhen Huang,Guoqiang Wei,Anqing Zheng,Haibin Li,Zengli Zhao 한국화학공학회 2017 Korean Journal of Chemical Engineering Vol.34 No.6

The effects of Mn substitution of LaMnxFe1−xO3 (x=0, 0.3, 0.5, 0.7, 1.0) on the oxidation activity and resistance to carbon formation for chemical-looping steam methane reforming (CL-SMR) were investigated. The desired crystalline perovskite phases were formed by transferring from the orthorhombic structure of LaFeO3 to rhombohedral lattice of LaMnO3 as the degree of Mn-doping increased. Manganese ions have a mixed state of Mn3+ and Mn4+ in the LaFe1−xMnxO3, meanwhile inducing the states of highly mixed character of Fe2+, Fe3+ and Fe4+ in iron ions. Substitution of Mn for Fe with proper value not only increases the lattice oxygen, which is conducive to the partial oxidation of CH4 to produce syngas, but also enhances the lattice oxygen mobility from the bulk to the surface of the oxygen carrier particles. Judging from the points of the redox reactivity, resistance to carbon formation and hydrogen generation capacity, the optimal range of the degree of Mn substitution is x=0.3-0.5.

LANE KEEPING CONTROL BASED ON MODEL PREDICTIVE CONTROL UNDER REGION OF INTEREST PREDICTION CONSIDERING VEHICLE MOTION STATES

Zeng Li,Gaojian Cui,Shaosong Li,Niaona Zhang,Yunsheng Tian,Xiaoqiang Shang 한국자동차공학회 2020 International journal of automotive technology Vol.21 No.4

To address the failure to consider vehicle states in region of interest (ROI) prediction, we propose the use of a Kalman filter to estimate the position of vehicles relative to lanes by vehicle states on the basis of a vehicle–road micro traffic model in the world coordinate system. The central position of the ROI is determined through a combination of optimal preview time theory with the ROI prediction. The range of the ROI is determined by offsetting upward, downward, leftward, and rightward from the central position of the ROI. The left and right ROI are processed separately to detect lane lines. Simulation results show that the proposed prediction method reduces the ROI range, and the model predictive control controller can make the vehicle run smoothly from the initial position to the road centerline.

The Soft Power of Culture: Economics Consideration Based on the Perspective of “Three Forces” of Culture

Zeng Li,Wu Nan,Li Baihan 아시아사회과학학회 2022 Jornal of Asia Social Science Vol.8 No.2

Focusing on “three forces” of cultural productivity, cultural competitiveness and cultural soft power, the paper considers “three forces” of culture from an economics perspective by constructing a linkage mechanism model of “three forces” of culture. From cultural resources to cultural capital into cultural productivit y, cultural productivity can be optimized through the balance between supply and demand. Then, the paper analyzes the construction of cultural competitiveness from perspective of cultural inheritance and innovation affecting the balance between supply and demand, efficiency. Combined with Michael Porter Diamond Model, it analyzes six factors that affect “three forces” of culture, such as enterprise strategy, related industries, demand conditions, production factors, opportunities and government. Therefore, “three forces” of culture mechanism aims at realizing the linkage mechanism of cultural economic value, cultural mechanism and social value, thus enhancing cultural soft power and national image.

Allusion as a Poetic Mode in Li Shangyin's Poetry on History

Zeng, Li 성균관대학교 동아시아학술원 2011 Sungkyun Journal of East Asian Studies Vol.11 No.1

A great deal of Li Shangyin's (ca. 812-858) poetry on history, a subgenre in Chinese classical verse, is outstanding among similar works by his contemporaries and by poets of previous ages. And in writing his poems on history, allusion served as an important mode for him. Centering round a major feature of his poetry on history, that is, the combination of traditional attitudes toward history with satire and irony, this article tackles the poet's artful manipulation of different modes of reading allusion, especially historical allusions. Through an analysis of selections from his allusive poems on historical themes, the article reveals how Li Shangyin's poetry on history produces a metaphorical and allegorical effect while using historical events as a moral mirror to reflect the political present.

New Construction Method for Quaternary Aperiodic, Periodic, and Z-Complementary Sequence Sets

Zeng, Fanxin,Zeng, Xiaoping,Zhang, Zhenyu,Zeng, Xiangyong,Xuan, Guixin,Xiao, Lingna The Korea Institute of Information and Commucation 2012 Journal of communications and networks Vol.14 No.3

Based on the known binary sequence sets and Gray mapping, a new method for constructing quaternary sequence sets is presented and the resulting sequence sets' properties are investigated. As three direct applications of the proposed method, when we choose the binary aperiodic, periodic, and Z-complementary sequence sets as the known binary sequence sets, the resultant quaternary sequence sets are the quaternary aperiodic, periodic, and Z-complementary sequence sets, respectively. In comparison with themethod proposed by Jang et al., the new method can cope with either both the aperiodic and periodic cases or both even and odd lengths of sub-sequences, whereas the former is only fit for the periodic case with even length of sub-sequences. As a consequence, by both our and Jang et al.'s methods, an arbitrary binary aperiodic, periodic, or Z-complementary sequence set can be transformed into a quaternary one no matter its length of sub-sequences is odd or even. Finally, a table on the existing quaternary periodic complementary sequence sets is given as well.