8-60hIPP5^m-Induced G2/M Cell Cycle Arrest Involves Activation of ATM/p53/p21^cip1/waf1 Pathways and Delayed Cyclin B1 Nuclear Translocation

Zeng, Qi-Yan,Zeng, Lin-Jie,Huang, Yu,Huang, Yong-Qi,Zhu, Qi-Fang,Liao, Zhi-Hong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.9

Protein phosphatase 1 (PP1) is a major serine/threonine phosphatase that controls gene expression and cell cycle progression. The active mutant IPP5 ($8-60hIPP5^m$), the latest member of the inhibitory molecules for PP1, has been shown to inhibit the growth of human cervix carcinoma cells (HeLa). In order to elucidate the underlying mechanisms, the present study assessed overexpression of $8-60hIPP5^m$ in HeLa cells. Flow cytometric and biochemical analyses showed that overexpression of $8-60hIPP5^m$ induced G2/M-phase arrest, which was accompanied by the upregulation of cyclin B1 and phosphorylation of G2/M-phase proteins ATM, p53, $p21^{cip1/waf1}$ and Cdc2, suggesting that $8-60hIPP5^m$ induces G2/M arrest through activation of the ATM/p53/$p21^{cip1/waf1}$/Cdc2/cyclin B1 pathways. We further showed that overexpression of $8-60hIPP5^m$ led to delayed nuclear translocation of cyclin B1. $8-60hIPP5^m$ also could translocate to the nucleus in G2/M phase and interact with $pp1{\alpha}$ and Cdc2 as demonstrated by co-precipitation assay. Taken together, our data demonstrate a novel role for $8-60hIPP5^m$ in regulation of cell cycle in HeLa cells, possibly contributing to the development of new therapeutic strategies for cervix carcinoma.

Sensitization of Cervical Carcinoma Cells to Paclitaxel by an IPP5 Active Mutant

Zeng, Qi-Yan,Huang, Yu,Zeng, Lin-Jie,Huang, Min,Huang, Yong-Qi,Zhu, Qi-Fang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.19

Paclitaxel is one of the best anticancer agents that has been isolated from plants, but its major disadvantage is its dose-limiting toxicity. In this study, we obtained evidence that the active mutant IPP5 ($8-60hIPP5^m$), the latest member of the inhibitory molecules for protein phosphatase 1, sensitizes human cervix carcinoma cells HeLa more efficiently to the therapeutic effects of paclitaxel. The combination of $8-60hIPP5^m$ with paclitaxel augmented anticancer effects as compared to paclitaxel alone as evidenced by reduced DNA synthesis and increased cytotoxicity in HeLa cells. Furthermore, our results revealed that $8-60hIPP5^m$ enhances paclitaxel-induced G2/M arrest and apoptosis, and augments paclitaxel-induced activation of caspases and release of cytochrome C. Evaluation of signaling pathways indicated that this synergism was in part related to downregulation of NF-${\kappa}B$ activation and serine/threonine kinase Akt pathways. We noted that $8-60hIPP5^m$ downregulated the paclitaxel-induced NF-${\kappa}B$ activation, $I{\kappa}B{\alpha}$ degradation, PI3-K activity and phosphorylation of the serine/threonine kinase Akt, a survival signal which in many instances is regulated by NF-${\kappa}B$. Together, our observations indicate that paclitaxel in combination with $8-60hIPP5^m$ may provide a therapeutic advantage for the treatment of human cervical carcinoma.

New Glycosides from Dracocephalum tanguticum Maxim

Qi Zeng,Hui-zi Jin,Ruijie Chang,Jiangjiang Qin,Xiangrong Cheng,Weidong Zhang 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.12

Four new glycosides, luteolin-7-methoxy-3'-O-(3''-O-acetyl)-β-D-gluco pyranuronic acid-6''-methyl ester (1), benzyl-6-[(2E)-2-butenoate]-β-D-glucopyranoside (2), 2-methoxy-4-(2-propen-1-yl)penyl-6-acetate-β-D-glucopyranoside (3), and 2-methoxy-4-(2-propen-1-yl)penyl-6-[(2E)-2-butenoate]-β- D-glucopyranoside (4), along with benzyl-β-D-glucopyranoside (5), 2-methoxy-4-(2-propen-1-yl)penyl-β-D-glucopyranoside (6), and pectolarigenin (7), were isolated from the whole plant of Dracocephalum tanguticum Maxim. The structures of 1-4 were elucidated by detailed spectroscopic analyses, including HR-ESI-MS and 2D NMR spectroscopic data. The inhibitory effects against nitric oxide production in LPS-stimulated RAW264.7 cells of all seven compounds were also evaluated.

Preparation of Poly{styene-co-4-(4-vinylphenoxy) phthalonitrile} Microspheres by a New Approach of “Co-Dissolution” and its Function Development

Qi Bian,Ke Zeng,Kai Qiu,Jiaojian Liu,Yancun Niu,Yancui Liu,Jianghuai Hu,Gang Yang 한국고분자학회 2015 Macromolecular Research Vol.23 No.7

Solid vinyl monomer with functional group “phthalonitrile” was successfully introduced into microspheres to make poly{styene-co-4-(4-vinylphenoxy) phthalonitrile} microspheres (PSPMs) with uniform (UPSPMs) and core-shell (core-shell PSPMs) structure using soap-free emulsion polymerization by the new approach of “codissolution”. The core-shell PSPMs then were used as the design platform to make metallophthalocyanine-containing microspheres. EA, FTIR, SEM, TEM, UV-vis, TGA, XPS, solid 1H NMR and XRD techniques were employed to analyze the formation and morphology of PSPMs and metallophthalocyanine-containing microspheres. The results showed that UPSPMs and core-shell PSPMs were both realized and they were regular sphericities with diameters of around 370 nm. The percentages of reacting weight of 4-(4-vinylphenoxy) phthalonitrile were close to 50%. Based on the design platform of core-shell PSPMs, metallophthalocyanine-containing microspheres could be obtained and the content of metallophthalocyanine was close to 19%.

Behavior of Al/Cu/Ti electrodes in surface acoustic wave filter at high power

Qi Li,Sulei Fu,Zengtian Lu,Lirong Qian,Rui Wang,Tongjin Chen,Cheng Song,Fei Zeng,Weibiao Wang,Feng Pan 한국물리학회 2019 Current Applied Physics Vol.19 No.4

Driven by the need for high data-rates and continuous reduction in device size, surface acoustic wave filters are required to work under increasingly high power. In this work, a series of 2.7 GHz surface acoustic wave filters with Al/Cu/Ti three-layered electrode were fabricated and loaded with high power. Those three-layered electrodes showed weaker texture but higher stability than Al-Cu alloy electrode at high power. Morphologies, microstructures and elements distribution in cross section of fingers were analyzed carefully before and after high power loading. Results show that the circular-arc-shaped outline of fingers were appeared in most samples after high power loading, and the number of gains in the finger cross section changed from some into several with much larger volume. The features of distribution of Cu atoms also coincided with these microstructures. By finite element method and phase diagram analysis, the higher stability of Al/Cu/Ti three-layered electrodes are attributed to precipitation of θ-CuAl2 in the bottom edge of electrode finger and Cu-doped α-Al in the center top.

Push–Pull Type Oligo(<i>N</i>-annulated perylene)quinodimethanes: Chain Length and Solvent-Dependent Ground States and Physical Properties

Zeng, Zebing,Lee, Sangsu,Son, Minjung,Fukuda, Kotaro,Burrezo, Paula Mayorga,Zhu, Xiaojian,Qi, Qingbiao,Li, Run-Wei,Navarrete, Juan T. Ló,pez,Ding, Jun,Casado, Juan,Nakano, Masayoshi,Kim, Dongho American Chemical Society 2015 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.137 No.26

<P>Research on stable open-shell singlet diradicaloids recently became a hot topic because of their unique optical, electronic, and magnetic properties and promising applications in materials science. So far, most reported singlet diradicaloid molecules have a symmetric structure, while asymmetric diradicaloids with an additional contribution of a dipolar zwitterionic form to the ground state were rarely studied. In this Article, a series of new push pull type oligo(N-annulated perylene)quinodimethanes were synthesized. Their chain length and solvent-dependent ground states and physical properties were systematically investigated by various experimental methods such as steady-state and transient absorption, two-photon absorption, X-ray crystallographic analysis, electron spin resonance, superconducting quantum interference device, Raman spectroscopy, and electrochemistry. It was found that with extension of the chain length, the diradical character increases while the contribution of the zwitterionic form to the ground state becomes smaller. Because of the intramolecular charge transfer character, the physical properties of this push pull system showed solvent dependence. In addition, density functional theory calculations on the diradical character and Hirshfeld charge were conducted to understand the chain length and solvent dependence of both symmetric and asymmetric systems. Our studies provided a comprehensive understanding on the fundamental structure and environment property relationships in the new asymmetric diradicaloid systems.</P>

Learning-Based Multiple Pooling Fusion in Multi-View Convolutional Neural Network for 3D Model Classification and Retrieval

Zeng, Hui,Wang, Qi,Li, Chen,Song, Wei Korea Information Processing Society 2019 Journal of information processing systems Vol.15 No.5

We design an ingenious view-pooling method named learning-based multiple pooling fusion (LMPF), and apply it to multi-view convolutional neural network (MVCNN) for 3D model classification or retrieval. By this means, multi-view feature maps projected from a 3D model can be compiled as a simple and effective feature descriptor. The LMPF method fuses the max pooling method and the mean pooling method by learning a set of optimal weights. Compared with the hand-crafted approaches such as max pooling and mean pooling, the LMPF method can decrease the information loss effectively because of its "learning" ability. Experiments on ModelNet40 dataset and McGill dataset are presented and the results verify that LMPF can outperform those previous methods to a great extent.

Adsorption-coupled reduction of bromate by Fe(II)–Al(III) layered double hydroxide in fixed-bed column: Experimental and breakthrough curves analysis

Qi Yang,Yu Zhong,Xiaoming Li,Xin Li,Kun Luo,Xiuqiong Wu,Hongbo Chen,Yang Liu,Guangming Zeng 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.28 No.-

In this study, the bromate removal was investigated in continuous fixed-bed column using Fe(II)–Al(III)layered double hydroxide (LDH). With increase of column bed depth from 1.0 to 3.0 cm, breakpoint time(tb) increased from 51 to 175 h while throughput volume raised from 12.24 to 42.00 L at breakthroughpoint. The bromate removal was attributed to the reduction of Fe(II) present in LDH. The breakthroughcurve was simulated well by Thomas model, but BDST model was the only effective to initial part(1–10%). The maximum removal capacity (N0) calculated by Thomas model reached 71.01 mmol/g atflow rate (3 mL/min).

Myeloid-Derived Suppressor Cells Are Associated with Viral Persistence and Downregulation of TCR ζ Chain Expression on CD8⁺ T Cells in Chronic Hepatitis C Patients

Zeng, Qing-Lei,Yang, Bin,Sun, Hong-Qi,Feng, Guo-Hua,Jin, Lei,Zou, Zheng-Sheng,Zhang, Zheng,Zhang, Ji-Yuan,Wang, Fu-Sheng Korean Society for Molecular and Cellular Biology 2014 Molecules and cells Vol.37 No.1

Myeloid-derived suppressor cells (MDSCs) play an important role in impairing the function of T cells. We characterized MDSCs in two chronic hepatitis C (CHC) cohorts: a cross-sectional group that included 61 treatment-naive patients with CHC, 14 rapid virologic response (RVR) cases and 22 early virologic response (EVR) cases; and a longitudinal group of 13 cases of RVR and 10 cases of EVR after pegylated-interferon-${\alpha}$/ribavirin treatment for genotype 1b HCV infection. Liver samples from 32 CHC patients and six healthy controls were subjected to immunohistochemical analysis. MDSCs frequency in treatment-naive CHC was significantly higher than in RVR, EVR, or healthy subjects and was positively correlated with HCV RNA. Patients infected with HCV genotype 2a had a significantly higher frequency of MDSCs than those infected with genotype 1b. Decreased T cell receptor (TCR) ${\zeta}$ expression on $CD8^+$ T cells was significantly associated with an increased frequency of MDSCs in treatment-naive CHC patients and was restored by L-arginine treatment in vitro. Increased numbers of liver arginase-$1^+$ cells were closely associated with the histological activity index in CHC. The TCR ${\zeta}$ chain was significantly downregulated on hepatic $CD8^+$ T cells in CHC. During antiviral follow up, MDSCs frequency in peripheral blood mononuclear cells was directly correlated with the HCV RNA load in the plasma and inversely correlated with TCR ${\zeta}$ chain expression in $CD8^+$ T cells in both RVR and EVR cases. Notably, the RVR group had a higher frequency of MDSCs at baseline than the EVR group. Collectively, this study provides evidence that MDSCs might be associated with HCV persistence and downregulation of CD8 ${\zeta}$ chain expression.

— Invited Review — Understanding the functionality of the rumen microbiota: searching for better opportunities for rumen microbial manipulation

Qi Wenlingli,Xue Ming-Yuan,Jia Ming-Hui,Zhang Shuxian,Yan Qiongxian,Sun Hui-Zeng 아세아·태평양축산학회 2024 Animal Bioscience Vol.37 No.2

Rumen microbiota play a central role in the digestive process of ruminants. Their remarkable ability to break down complex plant fibers and proteins, converting them into essential organic compounds that provide animals with energy and nutrition. Research on rumen microbiota not only contributes to improving animal production performance and enhancing feed utilization efficiency but also holds the potential to reduce methane emissions and environmental impact. Nevertheless, studies on rumen microbiota face numerous challenges, including complexity, difficulties in cultivation, and obstacles in functional analysis. This review provides an overview of microbial species involved in the degradation of macromolecules, the fermentation processes, and methane production in the rumen, all based on cultivation methods. Additionally, the review introduces the applications, advantages, and limitations of emerging omics technologies such as metagenomics, metatranscriptomics, metaproteomics, and metabolomics, in investigating the functionality of rumen microbiota. Finally, the article offers a forward-looking perspective on the new horizons and technologies in the field of rumen microbiota functional research. These emerging technologies, with continuous refinement and mutual complementation, have deepened our understanding of rumen microbiota functionality, thereby enabling effective manipulation of the rumen microbial community.