Simulation study of magnetorheological testing cell design by incorporating all basic operating modes

Mughni, Mohd J.,Mazlan, Saiful A.,Zamzuri, Hairi,Yazid, Izyan I.M.,Rahman, Mohd A.A. Techno-Press 2014 Smart Structures and Systems, An International Jou Vol.14 No.5

Magnetorheological (MR) fluid is one of the field-responsive fluids that is of interest to many researchers due to its high yield stress value, which depends on the magnetic field strength. Similar to electrorheological (ER) fluid, the combination of working modes is one of the techniques to increase the performance of the fluids with limited focus on MR fluids. In this paper, a novel MR testing cell incorporated with valve, shear and squeeze operational modes is designed and constructed in order to investigate the behaviour of MR fluid in combined mode. The magnetic field distribution in the design concept was analyzed using finite element method in order to verify the effective areas of each mode have the acceptable range of flux density. The annular gap of valve and shear were fixed at 1 mm, while the squeeze gap between the parallel circular surfaces was varied up to 20 mm. Three different coil configurations, which were made up from 23 SWG copper wires were set up in the MR cell. The simulation results indicated that the magnetic field distributed in the squeeze gap was the highest among the other gaps with all coils were subjected to a constant applied current of 1 A. Moreover, the magnetic flux densities in all gaps were in a good range of magnitude based on the simulations that validated the proposed design concept. Hence, the 3D model of the MR testing cell was designed using Solidworks for manufacturing processes.

Expression Profile of Genes Modulated by Aloe emodin in Human U87 Glioblastoma Cells

Haris, Khalilah,Ismail, Samhani,Idris, Zamzuri,Abdullah, Jafri Malin,Yusoff, Abdul Aziz Mohamed Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.11

Glioblastoma, the most aggressive and malignant form of glioma, appears to be resistant to various chemotherapeutic agents. Hence, approaches have been intensively investigated to targeti specific molecular pathways involved in glioblastoma development and progression. Aloe emodin is believed to modulate the expression of several genes in cancer cells. We aimed to understand the molecular mechanisms underlying the therapeutic effect of Aloe emodin on gene expression profiles in the human U87 glioblastoma cell line utilizing microarray technology. The gene expression analysis revealed that a total of 8,226 gene alterations out of 28,869 genes were detected after treatment with $58.6{\mu}g/ml$ for 24 hours. Out of this total, 34 genes demonstrated statistically significant change (p<0.05) ranging from 1.07 to 1.87 fold. The results revealed that 22 genes were up-regulated and 12 genes were down-regulated in response to Aloe emodin treatment. These genes were then grouped into several clusters based on their biological functions, revealing induction of expression of genes involved in apoptosis (programmed cell death) and tissue remodelling in U87 cells (p<0.01). Several genes with significant changes of the expression level e.g. SHARPIN, BCAP31, FIS1, RAC1 and TGM2 from the apoptotic cluster were confirmed by quantitative real-time PCR (qRT-PCR). These results could serve as guidance for further studies in order to discover molecular targets for the cancer therapy based on Aloe emodin treatment.

Magnetic circuit optimization in designing Magnetorheological damper

Izyan I.M. Yazid,Saiful A. Mazlan,Fitrian Imaduddin,Takehito Kikuchi,Hairi Zamzuri 국제구조공학회 2014 Smart Structures and Systems, An International Jou Vol.14 No.5

This paper presents the materials analysis for combination of working modes of Magnetorheological (MR) damper. The materials were selected based on the optimum magnetic field strength at the effective areas in order to obtain a better design of MR damper. The design of electromagnetic circuit is one of the critical criteria in designing MR dampers besides the working mechanism and the types of MR damper. The increase in the magnetic field strength is an indication of the improvement in the damping performance of the MR damper. Eventually, the experimental test was performed under quasi-static loading to observe the performances of MR damper in shear mode, squeeze mode and mixed mode. The results showed that the increment of forces was obtained with the increased current due to higher magnetic flux density generated by electromagnetic coils. In general, it can be summarized that the combination of modes generates higher forces than single mode for the same experimental parameters throughout the study.

Association of The IDH1 C.395G>A (R132H) Mutation with Histological Type in Malay Brain Tumors

Yusoff, Abdul Aziz Mohamed,Zulfakhar, Fatin Najwa,Sul'ain, Mohd Dasuki,Idris, Zamzuri,Abdullah, Jafri Malin Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.12

Background: Brain tumors, constituting one of the most deadly forms of cancer worldwide, result from the accumulation of multiple genetic and epigenetic alterations in genes and signaling pathways. Isocitrate dehydrogenase enzyme isoform 1 (IDH1) mutations are frequently identified in primary brain tumors and acute myeloid leukemia. Studies on IDH1 gene mutations have been extensively performed in various populations worldwide but not in Malaysia. This work was conducted to study the prevalence of IDH1 c.395G>A (R132H) hotspot mutations in a group of Malaysian patients with brain tumors in order to gain local data for the IDH1 mutation profile in our population. Methods: Mutation analysis of c.395G>A (R132H) of IDH1 was performed in 40 brain tumor specimens by the polymerase chain reaction-restriction fragment length polymorphism method (PCR-RFLP) and then verified by direct sequencing. Associations between the IDH1 c.395G>A (R132H) mutation and clinicopathologic characteristics were also analyzed. Results: The IDH1 c.395G>A (R132H) mutation was detected in 14/40 patients (35%). A significant association was found with histological tumor types, but not with age, gender and race. Conclusions: IDH1 is frequently mutated and associated with histological subtypes in Malay brain tumors.

Magnetic circuit optimization in designing Magnetorheological damper

Yazid, Izyan I.M.,Mazlan, Saiful A.,Kikuchi, Takehito,Zamzuri, Hairi,Imaduddin, Fitrian Techno-Press 2014 Smart Structures and Systems, An International Jou Vol.14 No.5

This paper presents the materials analysis for combination of working modes of Magnetorheological (MR) damper. The materials were selected based on the optimum magnetic field strength at the effective areas in order to obtain a better design of MR damper. The design of electromagnetic circuit is one of the critical criteria in designing MR dampers besides the working mechanism and the types of MR damper. The increase in the magnetic field strength is an indication of the improvement in the damping performance of the MR damper. Eventually, the experimental test was performed under quasi-static loading to observe the performances of MR damper in shear mode, squeeze mode and mixed mode. The results showed that the increment of forces was obtained with the increased current due to higher magnetic flux density generated by electromagnetic coils. In general, it can be summarized that the combination of modes generates higher forces than single mode for the same experimental parameters throughout the study.

Simulation study of magnetorheological testing cell design by incorporating all basic operating modes

Mohd J. Mughni,Saiful A. Mazlan,Izyan I.M. Yazid,Mohd A.A. Rahman,Hairi Zamzuri 국제구조공학회 2014 Smart Structures and Systems, An International Jou Vol.14 No.5

Magnetorheological (MR) fluid is one of the field-responsive fluids that is of interest to many researchers due to its high yield stress value, which depends on the magnetic field strength. Similar to electrorheological (ER) fluid, the combination of working modes is one of the techniques to increase the performance of the fluids with limited focus on MR fluids. In this paper, a novel MR testing cell incorporated with valve, shear and squeeze operational modes is designed and constructed in order to investigate the behaviour of MR fluid in combined mode. The magnetic field distribution in the design concept was analyzed using finite element method in order to verify the effective areas of each mode have the acceptable range of flux density. The annular gap of valve and shear were fixed at 1 mm, while the squeeze gap between the parallel circular surfaces was varied up to 20 mm. Three different coil configurations, which were made up from 23 SWG copper wires were set up in the MR cell. The simulation results indicated that the magnetic field distributed in the squeeze gap was the highest among the other gaps with all coils were subjected to a constant applied current of 1 A. Moreover, the magnetic flux densities in all gaps were in a good range of magnitude based on the simulations that validated the proposed design concept. Hence, the 3D model of the MR testing cell was designed using Solidworks for manufacturing processes.

Mitochondrial 10398A>G NADH-Dehydrogenase Subunit 3 of Complex I Is Frequently Altered in Intra-Axial Brain Tumors in Malaysia

( Abdul Aziz Mohamed Yusoff ),( Fatin Najwa Zulfakhar ),( Siti Zulaikha Nashwa Mohd Khair ),( Wan Salihah Wan Abdullah ),( Jafri Malin Abdullah ),( Zamzuri Idris ) 대한뇌종양학회 대한신경종양학회 2018 Brain Tumor Research and Treatment Vol.6 No.1

Background Mitochondria are major cellular sources of reactive oxygen species (ROS) generation which can induce mitochondrial DNA damage and lead to carcinogenesis. The mitochondrial 10398A>G alteration in NADH-dehydrogenase subunit 3 (ND3) can severely impair complex I, a key component of ROS production in the mitochondrial electron transport chain. Alteration in ND3 10398A>G has been reported to be linked with diverse neurodegenerative disorders and cancers. The aim of this study was to find out the association of mitochondrial ND3 10398A>G alteration in brain tumor of Malaysian patients. Methods Brain tumor tissues and corresponding blood specimens were obtained from 45 patients. The ND3 10398A>G alteration at target codon 114 was detected using the PCR-RFLP analysis and later was confirmed by DNA sequencing. Results Twenty-six (57.8%) patients showed ND3 10398A>G mutation in their tumor specimens, in which 26.9% of these mutations were heterozygous mutations. ND3 10398A>G mutation was not significantly correlated with age, gender, and histological tumor grade, however was found more frequently in intra-axial than in extra-axial tumors (62.5% vs. 46.2%, p< 0.01). Conclusion For the first time, we have been able to describe the occurrence of ND3 10398A >G mutations in a Malaysian brain tumor population. It can be concluded that mitochondrial ND3 10398A>G alteration is frequently present in brain tumors among Malaysian population and it shows an impact on the intra-axial tumors.