Effect of Al on the Microstructure and Mechanical Properties of Mg–Sn–Ca–Mn Wrought Alloy

Yun Feng,Yuanyuan Yang,Zongqi Xiao,Xianglong Meng,Guorong Zhou,Jinfeng Leng,Xinying Teng 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.6

Mg–Sn–Ca–Mn–xAl (x = 0,1.5 wt%) alloys with ultra-fine recrystallized grains were prepared by conventional extrusion(400 °C, 0.5 mm/s). Effects of aluminum (Al) on mechanical properties and microstructure of the Mg–1Sn–1Ca–0.5Mn(wt%) alloy were systematically investigated. By the addition of 1.5 wt% Al, the yield strength of the as-extruded alloysignificantly increased from 183 to 237 MPa. The as-cast alloys show a dendritic structure consists of α-Mg, (Mg, Al)2Ca,and CaMgSn phases. The as-extruded Mg–1Sn–1Ca–0.5Mn–1.5Al alloys exhibit a bimodal grain structure composed ofdynamic recrystallized (DRXed) fine grains and coarse unDRXed grains. Compared with Al free alloy, a lot of nano-scaleplanar Al2Caand rectangle shape Al8Mn5precipitated in the as-extruded alloy with 1.5 wt% Al. Meanwhile, the addition ofAl significantly strengthened alloys’ fiber texture with < 10–10 >//ED.

Association Between Single Nucleotide Polymorphisms in the XRCC1 Gene and Susceptibility to Prostate Cancer in Chinese Men

Zhou, Yun-Feng,Zhang, Guang-Bo,Qu, Ping,Zhou, Jian,Pan, Hui-Xin,Hou, Jian-Quan Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.10

Background: Prostate cancer (Pca) is one of the most common complex and polygenic diseases in men. The X-ray repair complementing group 1 gene (XRCC1) is an important candidate in the pathogenesis of Pca. The purpose of this study was to evaluate the association between single nucleotide polymorphisms in the XRCC1 gene and susceptibility to Pca. Materials and Methods: XRCC1 gene polymorphisms and associations with susceptibility to Pca were investigated in 193 prostate patients and 188 cancer-free Chinese men. Results: The c.910A>G variant in the exon9 of XRCC1 gene could be detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and DNA sequencing methods. Significantly increased susceptibility to prostate cancer was noted in the homozygote comparison (GG versus AA: OR=2.95, 95% CI 1.46-5.42, ${\chi}^2$=12.36, P=0.001), heterozygote comparison (AG versus AA: OR=1.76, 95% CI 1.12-2.51, ${\chi}^2$=4.04, P=0.045), dominant model (GG/AG versus AA: OR=1.93, 95% CI 1.19-2.97, ${\chi}^2$=9.12, P=0.003), recessive model (GG versus AG+AA: OR=2.17, 95% CI 1.33-4.06, ${\chi}^2$=8.86, P=0.003) and with allele contrast (G versus A: OR=1.89, 95% CI 1.56-2.42, ${\chi}^2$=14.67, P<0.000). Conclusions: These findings suggest that the c.910A>G polymorphism of the XRCC1 gene is associated with susceptibility to Pca in Chinese men, the G-allele conferring higher risk.

Security Threats among DICOM Imaging Communications in Public Networks

Feng Zhou,Zhongqi Zhang,Jin Wang,Bin Li,Jeong-Uk Kim 보안공학연구지원센터 2014 International Journal of Future Generation Communi Vol.7 No.6

Picture archiving and communication systems (PACS) require high-speed networks to transmit large image files between components. Image-data transmission from one site to another through public network is usually characterized in term of privacy, authenticity, and integrity. However, public network’s security issues had always been the significant problems. Recent years, IPv6 brings significant improvements in mechanisms for assuring a higher level of security and confidentiality of the transmitted information. Thus, it is still necessary to take care of some particular aspects. In this paper, we first analyzes how actual security threats and different types of attacks affect IPv6 networks while transmitting Digital Imaging and Communications in Medicine (DICOM) files through the public Internet. Second, illustrate some shortcomings of IPv6 and IPv6’s traffic loads. Finally, some possible solutions against a number of security threats in IPv6 DICOM files transmitting networks have been given.

A novel M2e-multiple antigenic peptide providing heterologous protection in mice

Feng Wen,Ji-Hong Ma,Hai Yu,Fu-Ru Yang,Meng Huang,Yan-Jun Zhou,Ze-Jun Li,Xiu-Hui Wang,Guo-Xin Li,Yi-Feng Jiang,Wu Tong,Guangzhi Tong 대한수의학회 2016 Journal of Veterinary Science Vol.17 No.1

Swine influenza viruses (SwIVs) cause considerable morbidity and mortality in domestic pigs, resulting in a significant economic burden. Moreover, pigs have been considered to be a possible mixing vessel in which novel strains loom. Here, we developed and evaluated a novel M2e-multiple antigenic peptide (M2e-MAP) as a supplemental antigen for inactivated H3N2 vaccine to provide cross-protection against two main subtypes of SwIVs, H1N1 and H3N2. The novel tetra-branched MAP was constructed by fusing four copies of M2e to one copy of foreign T helper cell epitopes. A high-yield reassortant H3N2 virus was generated by plasmid based reverse genetics. The efficacy of the novel H3N2 inactivated vaccines with or without M2e-MAP supplementation was evaluated in a mouse model. M2e-MAP conjugated vaccine induced strong antibody responses in mice. Complete protection against the heterologous swine H1N1 virus was observed in mice vaccinated with M2e-MAP combined vaccine. Moreover, this novel peptide confers protection against lethal challenge of A/Puerto Rico/8/34 (H1N1). Taken together, our results suggest the combined immunization of reassortant inactivated H3N2 vaccine and the novel M2e-MAP provided cross-protection against swine and human viruses and may serve as a promising approach for influenza vaccine development.

Comparative genomic analysis of Mycoplasma anatis strains

Zhou Qi,Mai Kaijie,Yang Dehong,Liu Junfa,Yan Zhuanqiang,Luo Cuifen,Tan Yangtong,Cao Sheng,Zhou Qingfeng,Chen Li,Chen Feng 한국유전학회 2021 Genes & Genomics Vol.43 No.11

Background The Gram-negative intracellular bacterium Mycoplasma anatis is a pathogen of respiratory infectious diseases in ducks and has caused signifcant economic losses in the poultry industry. Objective This study, as the frst report of the structure and function of the pan-genome of Mycoplasma anatis, may provide a valuable genetic basis for many aspects of future research on the pathogens of waterfowl. Methods We sequenced the whole genomes of 15 Mycoplasma anatis isolated from ducks in China. Draft genome sequencing was carried out and whole-genome sequencing was performed by the sequencers of the PacBio Sequel and an IonTorrent Personal Genome Machine (PGM). Then the common genic elements of protein-coding genes, tRNAs, and rRNAs of Mycoplasma anatis genomes were predicted by using the pipeline Prokka v1.13.7. To investigate homologous protein clusters across Mycoplasma anatis genomes, we adopted Roary v3.13.0 to cluster orthologous genes (OGs) based on the following criteria. Results We obtained one complete genome and 14 genome sketches. Microbial mobile genetic element analysis revealed the distribution of insertion sequences (IS30, IS3, and IS1634), prophage regions, and CRISPR arrays in the genome of Mycoplasma anatis. Comparative genomic analysis decoded the genetic components and functional classifcation of the pan-genome of Mycoplasma anatis that comprised 646 core genes, 231 dispensable genes and among them 110 was strainspecifc. Virulence-related gene profles of Mycoplasma anatis were systematically identifed, and the products of these genes included bacterial ABC transporter systems, iron transport proteins, toxins, and secretion systems. Conclusion A complete virulence-related gene profle of Mycoplasma anatis has been identifed, most of the genes are highly conserved in all strains. Sequencing results are relevant to the molecular mechanisms of drug resistance, adaptive evolution of pathogens, population structure, and vaccine development.

Experimental and Numerical Investigations of High Strength Steel Welded H-section Columns

Feng Zhou,Le-Wei Tong,Yiyi Chen 한국강구조학회 2013 International Journal of Steel Structures Vol.13 No.2

This paper reports experimental and numerical investigations of high strength steel columns. A series of tests was performed on different geometries of welded H-sections fabricated from high strength steel sheet with a nominal yield stress of 460 MPa. A non-linear finite element model which includes geometric and material non-linearities was developed and verified against experimental results of high strength steel welded H-section columns. The calibrated model was shown to provide accurate predictions of the experimental ultimate loads and failure modes of the test specimens. Therefore, a parametric study was carried out over a range of cross-section geometries and column lengths. The test and numerical results of stub columns obtained in this study were compared with the nominal section capacities. It is shown that the design provisions specified in the European Code, American Specification and Chinese Code on yield slenderness limits are all conservative. The normalized flange and web slenderness limits for fully effective section codified in European Code are very close to those codified in the American Specification, which are more suitable. Furthermore, the test and numerical results of long columns obtained in this study were compared with the nominal member capacities predicted using the European Code, American Specification and Chinese Code for steel structures. It is shown that the European Code provides the best agreement between the test and numerical data with design strength predictions for high strength steel welded H-section long columns.

Hierarchical Colored Petri Nets Based Components for Workflow Systems

Feng Zhou,Rui-Feng Bo,Hong-Zhong Huang,Dan Ling,Qiang Miao 대한기계학회 2006 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.20 No.10

Workflow systems have benefited the enterprise and customer in many aspects. But with the increasing complexity of the system, workflow design becomes a complicated and time-consuming process. In this paper, we model the system based on the Hierarchical Colored Petri Nets (HCPN) to avoid the complexity of the system, and the super nets of the model are abstracted as independent components to increase the flexibility, acceptability and maintainability of the system. Another important characteristic of this model is its convenience for reuse and workflow mining. The development process of the overhead traveling crane was given to demonstrate the proposed method.

Pseudo-homogeneous kinetic modeling of dioctyl terephthalate (DOTP) production by esterification of terephthalic acid and 2-ethylhexanol over tetrabutyl titanate catalyst

Feng Zhou,Jinjin Cai,Xiaoning Mao,Zhenyu Wu,Yong Nie 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.9

As a green plasticizer, the industrial production of dioctyl terephthalate (DOTP) is still facing the problemof high energy consumption. To optimize the production process and reactor, it is essential to understand the kineticbehavior of reaction system. In this work, the two-step consecutive esterification of solid terephthalic acid (PTA) and 2-ethylhexanol (2-EH) catalyzed by tetrabutyl titanate was studied. First, the equilibrium constants and enthalpies of thetwo-step reaction were experimentally determined and validated by the group contribution methods. Then, a pseudohomogeneouskinetic model was developed, and the reaction order of PTA was corrected to reflect its solid phase characteristic. Non-isothermal kinetic experiments were carried out under different initial feed molar ratios and catalystconcentrations, and the kinetic parameters in the model were estimated by mathematical regression. The model predicteddata agreed well with the experimental data. Finally, the analyses of reaction rate showed that the first-step reactionwas the rate-controlling step of the whole esterification process.

Apoptotic Effects of psiRNA-STAT3 on 4T1 Breast Cancer Cells in Vitro

Zhou, Yue,Tian, Lin,Zhang, Ying-Chao,Guo, Bao-Feng,Zhou, Qing-Wei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.16

Background: The aim of this study was to investigate the effect of a Lipofectamine2000 (Life2000) Transfection Reagent transfected psiRNA-STAT3 plasmid on 4T1 breast cancer cells. Materials and Methods: MTT was used to detect the cell proliferation of breast cancer 4T1 cells at different periods (0h, 6h, 8h, 10h); the cell cycle was assessed by flow cytometry; variation of apoptosis and mitochondrial membrane potential was observed under a fluorescence microscope; immunohistochemical staining was used to determine the expression of caspase-3 and cyclin-D1 protein. Results: An obvious effect of inhibition to 4T1 cancer cells could be observed at 8h after the psiRNA-STAT3 was transfected. Typical alterations of apoptotic morphological features were visible in the psiRNA-STAT3 treatment group. Mitochondrial membrane potential decreased significantly, the number of cells was increased in G0/G1 phase, and the number of cells was decreased in S phase, and the data were statistically significant (p<0.05), compared with the Scramble and Mock groups. Expression of caspase-3 protein was increased significantly, while that of cyclin D1 was significantly decreased. Conclusions: Life2000 transfected psiRNA-STAT3 plasmid can inhibit 4T1 tumor cell proliferation and promote apoptosis of 4T1 tumor cells, which process depends on the regulation of expression of cyclin D1 and caspase-3 protein.

Fabrication of Poly(styrene-co-acrylic acid)/Polystyrene Anisotropic Nanoparticles via Seeded Emulsion Polymerization

Feng Zhou,Shaohui Lin,Garry L. Rempel,Qinmin Pan 한국고분자학회 2017 폴리머 Vol.41 No.6

Anisotropic nanoparticles have potentially various important applications, especially in 3D printing materials, binders and phase compatible agents, for which it is essential that the components and the structures are well designed and controlled. This paper investigated nanoparticles consisting of two lobes with opposite properties. As a model system, synthesis of poly(styrene-co-acrylic acid)/polystyrene anisotropic nanoparticles was studied and the effects of temperature, surfactant amount, inhibitors, and initiators for the involved polymerization procedures, and the swelling temperature, and swelling ratio for making the second lobe, on the morphology and size of the produced particles , were investigated. As a result, the anisotropic nanoparticles with uniform asymmetry, one lobe with hydrophilicity and the other with hydrophobicity, were successfully realized. The apparent size of the nanoparticles was below 200 nm, and the strategies for achieving various morphologies, such as snowman-like, potato-like and dumbbell-like, were established. The anisotropic nanoparticles achieved in this study are very useful in the 3D material development that is involved in our another research.