Active hexose correlated compound potentiates the antitumor effects of low-dose 5-fluorouracil through modulation of immune function in hepatoma 22 tumor-bearing mice

Zhiyun Cao,Xuzheng Chen,Lan Lan,Zhideng Zhang,Jian Du,Lianming Liao 한국영양학회 2015 Nutrition Research and Practice Vol.9 No.2

BACKGROUND/OBJECTIVES: A variety of immunomodulators can improve the efficacy of low-dose chemotherapeutics. Active hexose correlated compound (AHCC), a mushroom mycelia extract, has been shown to be a strong immunomodulator. Whether AHCC could enhance the antitumor effect of low-dose 5-fluorouracil (5-FU) via regulation of host immunity is unknown. MATERIALS/METHODS: In the current study Hepatoma 22 (H22) tumor-bearing mice were treated with PBS, 5-FU (10 mg?kg<SUP>-1</SUP>?d<SUP>-1</SUP>, i.p), or AHCC (360 mg?kg<SUP>-1</SUP>?d<SUP>-1</SUP>, i.g) plus 5-FU, respectively, for 5 d. CD3<SUP>+</SUP>, CD4<SUP>+</SUP>, CD8<SUP>+</SUP>, and NK in peripheral blood were detected by flow cytometry. ALT, AST, BUN, and Cr levels were measured by biochemical assay. IL-2 and TNFα in serum were measured using the RIA kit and apoptosis of tumor was detected by TUNEL staining. Bax, Bcl-2, and TS protein levels were measured by immunohistochemical staining and mRNA level was evaluated by RT-PCR. RESULTS: Diet consumption and body weight showed that AHCC had no apparent toxicity. AHCC could reverse liver injury and myelosuppression induced by 5-FU (P < 0.05). Compared to mice treated with 5-FU, mice treated with AHCC plus 5-FU had higher thymus index, percentages of CD3<SUP>+</SUP>, CD4<SUP>+</SUP>, and NK cells (P < 0.01), and ratio of CD4<SUP>+</SUP>/CD8<SUP>+</SUP> (P < 0.01) in peripheral blood. Radioimmunoassay showed that mice treated with AHCC plus 5-FU had the highest serum levels of IL-2 and TNFα compared with the vehicle group and 5-FU group. More importantly, the combination of AHCC and 5-FU produced a more potent antitumor effect (P < 0.05) and caused more severe apoptosis in tumor tissue (P < 0.05) compared with the 5-FU group. In addition, the combination of AHCC and 5-FU further up-regulated the expression of Bcl-2 associated X protein (Bax) (P< 0.01), while it down-regulated the expression of B cell lymphoma 2 (Bcl-2) (P < 0.01). CONCLUSIONS: These results support the claim that AHCC might be beneficial for cancer patients receiving chemotherapy.

A Modified Laminotomy for Interlaminar Endoscopic Lumbar Discectomy: Technical Report and Preliminary Results

Zhiyun Feng,Yuxu Wu,Honghao Wu,Tae Gyong Jon,Ying Yuan,Zhong Chen,Yue Wang 대한척추신경외과학회 2023 Neurospine Vol.20 No.4

Objective: To introduce a technique of laminotomy using a common trephine to enlarge the interlaminar space at L4/5 segment for interlaminar endoscopic lumbar discectomy (IELD) and report the anatomical basis of this procedure, technical details, as well as primary clinical outcomes of a consecutive patient cohort with L4/5 lumbar disc herniation (LDH). Methods: On anteroposterior fluoroscopy, the intersection of the medial edge of the inferior articular process and the inferior endplate of L4 vertebra was taken as the target. Using a common trephine, laminotomy was performed to remove a big portion of the posterior wall of the canal under the guidance of endoscopy. From June 2018 to December 2021, the consecutive patients who underwent L4/5 IELD were prospectively studied. Clinical outcomes were assessed at the day before surgery, 1 day, 1 month, 3 months, 12 months after surgery, and the last follow-up. Numerical Rating Scale, Roland-Morris Disability Questionnaire (RMDQ), and MacNab criteria were used to evaluate back and leg pain, the quality of life, and clinical efficacy, respectively. Results: There were 64 men and 44 women, with an age of 50.3 ± 14.9 years. The operating time was 74.54 ± 17.42 minutes. The mean follow-up time was 32.7 ± 18.6 months (range, 12–64 months). The complications of IELD included numbness, neck pain, and recurrence. Both leg pain (6.2 ± 1.9 vs. 1.8 ± 0.8, p < 0.001) and back pain (3.1 ± 2.3 vs. 1.7 ± 0.9, p < 0.001) quickly improved after this procedure and maintained (1.1 ± 1.5, 1.1 ± 1.3) at final follow-up. Physical disability due to back pain, as assessed using RMDQ, was improved remarkably after surgery (15.0 ± 5.8 vs. 2.9 ± 4.1, p < 0.001). In addition, MacNab outcome grade was evaluated as good-to-excellent in 96 cases (88.9%). Conclusion: A convenient technique of laminotomy using a common trephine was proposed for the L4/5 IELD. It can efficiently enlarge the interlaminar entry to perform endoscopic discectomy. This procedure is particularly suitable for treating LDH with concomitant lumbar spinal stenosis and migrated herniated disc.

In situ formation of NbSi₂/Nb₂O<SUB>5</SUB>-SiO₂/HfC multilayer oxidation protective coating by HAPC combined with PEO-assisted particle deposition and sintering

Zhiyun Ye,Shuqi Wang,Yongchun Zou,Guoliang Chen,Yaming Wang 한국표면공학회 2023 한국표면공학회 학술발표회 초록집 Vol.2023 No.11-1

Stigmast-4-en-6β-ol-3-one decreases viability and induces apoptosis and ferroptosis in liver cancer cells by reducing E2F1

Zhiyun Zhang,Jian Wang,Weiping Wan,Zhengchao Shen,Aixue Zuo,Rong Chen,Qinyi Wu,Enli Cai,Feng Huang,Rongping Zhang,Xinan Shi 한국통합생물학회 2023 Animal cells and systems Vol.27 No.1

Hepatocellular carcinoma (HCC) is a frequently occurring malignant gastrointestinal cancer. The 5-year survival rate of HCC is still below 8%, and thus, identifying more effective therapeutic methodsis needed. Here, we evaluated the effects of Stigmast-4-en-6β-ol-3-one (S463) on the viability andcolony formation of liver cancer cells. S463 treatment decreased the viability and inducedapoptosis and ferroptosis in liver cancer cells, and also increased cellular malondialdehyde(MDA) and lipid peroxidation levels. In S463 treated cells, the expression level of Bax wasincreased, and the expression level of GPX4 was reduced, and the cleavage of PARP wasimproved. We also found that S463 treatment downregulated E2F1 and upregulated p53 atboth the mRNA and protein levels. Importantly, rescue experiments revealed thatoverexpression of E2F1 partially restored S463-induced Bax and p53 upregulation and GPX4downregulation and counteracted the S463-induced decrease in cell viability and colonyformation and the S463-induced increase in MDA and lipid peroxidation levels. Our findingssuggest that S463 significantly inhibits viability and colony formation and induces apoptosisand ferroptosis in liver cancer cells via E2F1.

6.25전쟁기 한,중 전시소설에 드러난 포로형상에 대한 비교 연구

티엔즈윈 ( Zhiyun Tian ) 한국문학언어학회(구 경북어문학회) 2016 어문론총 Vol.68 No.-

6.25전쟁기에 전의를 고양시키려는 전시소설이 대량으로 창작되었다. 당시 승전의식을 높이는 데 영웅적 인물의 창출이 필연적으로 요청되었다. 그러나 대립된 양쪽의 사상을 동시에 접할 수 있는 존재로서 포로형상도 이데올로기의 선전에 중요한 수법으로 활용되고 있다. 1949년 제네바조약이 체결된 이후 포로에 대한 처우가 중요한 문제로 부상되었는데, 뒤이어 발생한 한국전쟁에서 이 포로의 처우문제가 중요하게 적용되었다. 따라서 포로에 대한 소설적 연구는 문학적인 측면에서 한층 더 중요성을 가진다고 볼 수 있다. 따라서 본고는 이번 전쟁에 직접 참석한 나라인 한국(남한)과 중국의 전시소설에 드러난 포로형상에 대해 비교 연구하고자 한다. 본고에서는 포로를 일반포로와 귀순포로로 나누어 살펴봤다. 한·중 양국 전시소설에서 일반포로에 대해 부정적인 모습으로 묘사한 점에 있어서 유사점을 보이지만, 중국은 풍자적인 수법으로 포로를 묘사하는 것과 달리 한국은 직설적으로 ‘한’을 담아 적군포로를 묘사한다는 측면에서 차이를 보인다. 또, 양국의 소설은 아군으로 ‘전향’ 온 포로에 대해 공동적으로 관대한 태도를 보이며 그들에게 피해자의 형상을 부여한다. 마지막으로 포로수용소(혹은 갇혀 있는 수용 공간)에서 포로들 사이에 형성된 생존을 위한 ‘자아-타자’ 역학관계를 분석했다. 한국 전시소설에서는 자의든 타의든 자기 이외의 사람을 고발함으로써 ‘자아와 타자’의 긴장 관계가 이루어진 것이라면 중국 전시소설에서는 흑인과 백인 간의 인종차별에 의해 조성된 ‘자아-타자’관계가 존재한다.이번 논의를 통해 같은 시기에 창작된 한·중 소설에 나타난 포로형상에 대해 보다 체계적인 인식을 발전시키는 기회가 되었고, 이데올로기를 준수하는 전제 아래 양국 전시소설에서 포로를 어떻게 형상화하였는지 문학작품의 자율성을 다시 한 번 인식하는 계기가 되었다. In the period of Korean war, lots of novels used to arouse the fighting spirit are created and here, the hero figures are needed. But as the one that can approach both of the oppositive ideologies , POW figure takes an important role in the war novels. Besides, after the conclusion of GPW, the treatment of POW is aroused again when comes to the Korea war. And it increased the necessity of novelistic study. Therefore, the purpose of this paper is to compare the POW figure in Korea and China`s Korea war novel. In this paper, the POW is divided into the ordinary POW and defected POW to be studied. It`s familiar for south Korea and China to describe the ordinary POW in negative method. But China`s war novel describe POW in a satirical way, which is different to the Korea`s war novel putting sorrow into the POW figures. And both in Korea and China`s war novel, there is a generous attitude toward the defected POW figure who change their ideological direction to the friendly army. And mostly the defected POW figure is created as sufferer. Finally, I tired to analyzed the ``ego-other`` dynamics relationship among the POWs. In south Korea, the ``ego-other`` relationship is made by reporting comrades beside himself(herself) and in China`s war novel, it`s the ``racial discrimination`` between the white and the black. I hope that , according to this paper, we can have more understanding about the war novel which created in the same period between Korea and China. Though, it cannot be free of the ideology, but also the novels have played an important role in arousing the victory in the war by creating a lot of figures. And we call this the autonomy of the literature.

한,중 6.25전쟁 전선소설에 드러난 “애정(愛情)의 정치적 서사” 비교연구

티엔즈윈 ( Zhiyun Tian ) 한국문학언어학회(구 경북어문학회) 2015 어문론총 Vol.66 No.-

한국전쟁은 당시 한중 양국의 문단 형성에 큰 영향을 미쳤다. 즉, 한국에서 종군작가단의 결성과 중국의 ``입조``(북한에 들어감) 작가단의 형성이 바로 그 결과물이다. 양국의 종군작가단은 전쟁의 승리에 대한 의식을 고취시키는 도구로서 큰 힘을 발휘하였다. 이른바 전쟁기의 문학은 강한 정치성을 띠게 되는 것이다. 이러한 전선소설에서 인간의 욕망을 대표하는 애정이라는 감정이 정치와 어떻게 연결되는지 살펴보고 전선소설 속에 있는 문학의 자율성을 탐지하는 것이본 논문의 목적이다. 본고에서 소개한 애정과 정치의 관계는 주로 ``개인적 애정과 조국애의 양보`` 관계와 ``개인적 애정과 조국애의 합일적 관계`` 두 가지 측면에서 접근이 가능하다. 해당 작품으로는 정비석의 「간호장교」와 양삭(楊朔)의 「삼천리강산」(三千里 江山)이 전자에 속하며 박영준의 「빨치산」과 노령(路翎)의 「저지대에서의 전투」(窪地上的戰役)가 후자에 속한다. 문학은 시대의 특수성으로부터 자유로울 수가 없다. 그러나 문학이 무조건 정치에만 부합하는 것도 아니기 때문에 정치상의 목적을 두고 있으면서도 다양한 서사수법을 사용한다는 점을 소홀히 해서는 안 된다. 이러한 지점에서 문학의 자율성 탐구가 요청된다. 아울러, 이 글에서 애정의 서사를 논의함을 통해 한중양국이 서로에 대한 인식의 고정관념을 깨뜨릴 수 있으면 한다. The outbreak of the Korean War had a great effect on Korea and China`s literature circle. That is, the organization of war writers in Korea and ``Ru Chao writers``(the writers who go to the north Korea) in China. As a ``weapon`` to encourage the victory, the war writers played an important role in Korea and China. So the literature took a political appearance during the wartime. Love represents one of human`s instincts. So the appearances of connection between love and war become an interesting subject. And also I want to show the relative independence of literature from the politics. This paper looked into the relationship between personal love and politics, and focused on two pieces mainly. One is personal love`s ``concession`` to national love. Another one is the realization of the national love through the help of personal love. In this paper, I chose Jeong Bee-suk`s 「nurse officer」, Park Young-Jun`s 「partisan」 and Yang shuo`s 「Korean Peninsula」, Lu ling`s 「The battle the mud puddle」 as the respective novels. As is generally known literature could be influenced by the period, especially the war period. But that`s not blind loyalty. we should not neglect that literature can also use various narration methods to realize the political purpose. Therefore we should pay attention to the relative independence of literature. And according to the paper, I hope that the prejudice to each other between Korea and China could be tore down.

Molecular cloning, characterization and expression analysis of bolting-associated genes in flowering Chinese cabbage

Xufeng Xiao,Caijun Wu,Zhiyun Xu,Yingui Yang,Shuying Fan,Heng Wang 한국유전학회 2015 Genes & Genomics Vol.37 No.4

Bolting and flowering enhance the commercial value of flowering Chinese cabbage. FLOWERING LOCUS C (FLC) and FRIGIDA (FRI) are two key flowering time genes in Arabidopsis thaliana. Here we reported on the cloning and characterization of three ‘classical’ genes from the autonomous pathway from flowering Chinese cabbage, BrcuFCA, BrcuFLD and BrcuFVE. The results of expression analysis showed BrcuFLC was a gradually up-regulated with the developmental stages. However, temporal mRNA expression of BrcuFRI, BrcuFCA, BrcuFVE, and BrcuFLD were found to follow the opposite transcription patterns. The spatial expression patterns of BrcuFCA, BrcuFLD, and BrcuFVE were similar with the highest levels in flowers, whereas the highest transcription levels of BrcuFLC occurred in leaves and stems and that of BrcuFRI in roots. We presumed that the main pathway of bolting– flowering regulation in flowering Chinese cabbage might be the autonomous pathway and different from the vernalization pathway and FRI-dependent pathway.

Multi-Tenant Data Storage Model and Performance Evaluation

Dun Li,Zhenfei Wang,Zhiyun Zheng,Jin Zhao 보안공학연구지원센터 2016 International Journal of Database Theory and Appli Vol.9 No.3

Multi-tenant data storage model has multiple solutions and comparing the different storage solutions can help users improve their work efficiency. This paper proposes a query performance evaluation method based on the relational algebra. First of all, we introduce three wide table models. Secondly, we unite the format of tenant query SQL statement by analyzing structure of storage model, replace the unified format SQL with the relational algebra and evaluate the I/O cost of SQL query using relational algebra. Finally, through theoretical calculations and experimental simulations, we evaluate the performance of multi-tenant storage model according to query performance. The results show our evaluation method based on relational algebra provides new perspective for the study of performance evaluation in multi-tenant data model.

Tourism Destination Recommender System for the Cold Start Problem

( Xiaoyao Zheng ),( Yonglong Luo ),( Zhiyun Xu ),( Qingying Yu ),( Lin Lu ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.7

With the advent and popularity of e-commerce, an increasing number of consumers prefer to order tourism products online. A recommender system can help these users contend with information overload; however, such a system is affected by the cold start problem. Online tourism destination searching is a more difficult task than others on account of its more restrictive factors. In this paper, we therefore propose a tourism destination recommender system that employs opinion-mining technology to refine user preferences and item opinion reputations. These elements are then fused into a hybrid collaborative filtering method by combining user- and item-based collaborative filtering approaches. Meanwhile, we embed an artificial interactive module in our recommender system to alleviate the cold start problem. Compared with several well-known cold start recommendation approaches, our method provides improved recommendation accuracy and quality. A series of experimental evaluations using a publicly available dataset demonstrate that the proposed recommender system outperforms existing recommender systems in addressing the cold start problem.

Prevalence of Plasmid-Mediated Quinolone Resistance Genes Among Escherichia coli in the Gut of Healthy People in Fuzhou, China

Bin Ling,Yao Chen,Zhiyun Wu,Zhichang Zhao,Juan Wu,Yingping Cao 대한진단검사의학회 2018 Annals of Laboratory Medicine Vol.38 No.4

The intestinal tract may be an important reservoir for antibioticresistant genes [1]. Determining the prevalence of quinolone resistance in intestinal bacteria within the community is important for both healthy subjects and hospital patients as quinolone is one of the most commonly used antibiotics in China. The prevalence of plasmid-mediated quinolone resistance (PMQR) geneharboring Enterobacteriaceae in the gut flora among healthy humans was previously unknown, so we assessed the prevalence of PMQR genes among commensal Escherichia coli in healthy persons from one region in China. This study was approved by the Ethics Committee of the Fujian Medical University Union Hospital (No. 2012KY085). Written informed consent was obtained from all participants.