Expression and Distribution of the Guanine Nucleotide-binding Protein Subunit Alpha-s in Mice Skin Tissues and Its Association with White and Black Coat Colors

Yin, Zhihong,Zhao, Xin,Wang, Zhun,Li, Zhen,Bai, Rui,Yang, Shanshan,Zhao, Min,Pang, Quanhai Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.10

Guanine nucleotide-binding protein subunit alpha-s ($Gn{\alpha}s$) is a small subunit of the G protein-couple signaling pathway, which is involved in the formation of coat color. The expression level and distribution of $Gn{\alpha}s$ were detected by quantitative real-time-polymerase chain reaction (qPCR), western blot, and immunohistochemistry to investigate the underlying mechanisms of coat color in white and black skin tissues of mice. qPCR and western blot results suggested that $Gn{\alpha}s$ was expressed at significantly higher levels in black mice compared with that of white mice, and transcripts and protein possessed the same expression in both colors. Immunohistochemistry demonstrated $Gn{\alpha}s$ staining in the root sheath and dermal papilla in hair follicle of mice skins. The results indicated that the $Gn{\alpha}s$ gene was expressed in both white and black skin tissues, and the expression level of $Gn{\alpha}s$ in the two types of color was different. Therefore, $Gn{\alpha}s$ may be involved in the coat color formation in mice.

Research on Modeling Improvement of Rubber Isolators by Considering Multi-loading Conditions

Yin-Yan Zhao,Wen-Bin Shangguan,Nong Cheng,Xiao-Jing He 한국정밀공학회 2013 International Journal of Precision Engineering and Vol. No.

The stress-strain relationships of a rubber material in uniaxial tension, equi-biaxial tension and plane shear loading conditions are tested here. Constitutive parameters in different constitutive models, such as Mooney-Rivlin model, Van Der Waals model and Marlow model can be obtained by least square method using measured and calculated stress-strain relations. The rubber isolators in two rubber mounts are investigated here. Rubber mount I bears stretching/compression deformation or shear deformation, while mount II bears both deformations simultaneously. Using obtained constitutive constants, the rubber isolators’ static stiffness values in three mutually perpendicular directions are calculated, analyzed and compared with their measured results. Based on Mooney-Rivlin model, the effects of these constitutive constants on static stiffness of the two mounts are discussed by comparison. The effects of different models on static force-displacement characteristics of rubber mount I are also analyzed. The conclusion is that it is necessary to choose a relatively accurate constitutive model and appropriate methods for obtaining the model constants in describing rubber characteristic.

Insulated Interlayer for Efficient and Photostable Electron-Transport-Layer-Free Perovskite Solar Cells

Zhao, Pengjun,Han, Manhyung,Yin, Wenping,Zhao, Xing,Kim, Seul Gi,Yan, Yaping,Kim, Minwoo,Song, Young Jae,Park, Nam Gyu,Jung, Hyun Suk American Chemical Society 2018 ACS APPLIED MATERIALS & INTERFACES Vol.10 No.12

<P>Currently, the most efficient perovskite solar cells (PSCs) mainly use planar and mesoporous titanium dioxide (TiO<SUB>2</SUB>) as an electron-transport layer (ETL). However, because of its intrinsic photocatalytic properties, TiO<SUB>2</SUB> can decompose perovskite absorber and lead to poor stability under solar illumination (ultraviolet light). Herein, a simplified architectural ETL-free PSC with enhanced efficiency and outstanding photostability is produced by the facile deposition of a bathocuproine (BCP) interlayer. Power conversion efficiency of the ETL-free PSC improves from 15.56 to 19.07% after inserting the BCP layer, which is the highest efficiency reported for PSCs involving an ETL-free architecture, versus 19.03% for the n-i-p full device using TiO<SUB>2</SUB> as an ETL. The BCP interlayer has been demonstrated to have several positive effects on the photovoltaic performances of devices, such as “modulation doping” of the perovskite layer, modification of FTO surface work function, and enhancing the charge-transfer efficiency between FTO and perovskite. Moreover, the BCP-based ETL-free devices exhibit outstanding photostability: the unencapsulated BCP-based ETL-free PSCs retain over 90% of their initial efficiencies after 1000 h of storage in air and maintain 92.2% after 450 h of exposure to full solar irradiation (without a UV filter), compared to only 14.1% in the n-i-p full cells under the same condition.</P> [FIG OMISSION]</BR>

Experimental study on the performance of a turbocompound diesel engine with variable geometry turbocharger

Yin, Yong,Liu, Zhengbai,Zhuge, Weilin,Zhao, Rongchao,Zhao, Yanting,Chen, Zhen,Mi, Jiao Korean Society for Fluid machinery 2016 International journal of fluid machinery and syste Vol.9 No.4

Turbocompounding is a key technology to satisfy the future requirements of diesel engine's fuel economy and emission reduction. A turbocompound diesel engine was developed based on a conventional 11-Liter heavy-duty diesel engine. The turbocompound system includes a power turbine, which is installed downstream of a Variable Geometry Turbocharger (VGT) turbine. The impacts of the VGT rack position on the turbocompound engine performance were studied. An optimal VGT control strategy was determined. Experimental results show that the turbocompound engine using the optimal VGT control strategy achieves better performance than the original engine under all full load operation conditions. The averaged and maximum reductions of the brake specific fuel consumption (BSFC) are 3% and 8% respectively.

Experimental study on the performance of a turbocompound diesel engine with variable geometry turbocharger

Yong Yin,Zhengbai Liu,Weilin Zhuge,Rongchao Zhao,Yanting Zhao,Zhen Chen,Jiao Mi 한국유체기계학회 2016 International journal of fluid machinery and syste Vol.9 No.4

Turbocompounding is a key technology to satisfy the future requirements of diesel engine’s fuel economy and emission reduction. A turbocompound diesel engine was developed based on a conventional 11-Liter heavy-duty diesel engine. The turbocompound system includes a power turbine, which is installed downstream of a Variable Geometry Turbocharger (VGT) turbine. The impacts of the VGT rack position on the turbocompound engine performance were studied. An optimal VGT control strategy was determined. Experimental results show that the turbocompound engine using the optimal VGT control strategy achieves better performance than the original engine under all full load operation conditions. The averaged and maximum reductions of the brake specific fuel consumption (BSFC) are 3% and 8% respectively.

Synthesis and Cytotoxicity of Novel 3-Amino-4-indolylmaleimide Derivatives

Sheng-Yin Zhao,Yan-Wu Yang,Hai-Quan Zhang,Yun Yue,Mei Fan 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.4

In an attempt to develop potent and selective antitumor agents, a series of novel 3-amino-4-indolylmaleimides were designed and synthesized. The reaction showed high regioselectivity. The structure of compound 7a was determined by an X-ray single crystal diffraction method. The cytotoxicities of the title compounds were evaluated against HeLa, SMMC 7721 and HL 60 cancer cell lines by a standard MTT assay in vitro. The pharmacological results showed that some of the title compounds displayed moderate or high cytotoxic activity against the tested cell lines. Compound 7d was the most promising compound against the tested cancer cell lines. Structure-activity relationships are discussed based on the experimental data obtained. A hydroxyethylamino group at the 3-position in the side chain of indolylmaleimide is associated with an increase in cytotoxicity.

중국인 한국어 학습자의 쓰기 불안 연구

조인(Zhao, Yin),김영주(Kim, Young-Joo) 경성대학교 인문과학연구소 2015 인문학논총 Vol.38 No.-

본 연구는 TOPIK 수준에 따라 중국인 한국어 학습자의 쓰기 불안 양상을 살펴보고 그 구성요인들을 분류하며, 쓰기 불안 구성요인들과 학습자의 개인적 변인간에 어떤 관계가 있는지 알아보는 데 그 목적이 있다. 따라서 본 연구는 한국 내 대학 부설 어학원, 학부 또는 대학원에 재학 중인 TOPIK 3급 이상 학습자 130명을 대상으로 설문조사와 심층인터뷰를 실시하였다. 결과는 다음과 같다. (1) 대부분의 중국인 한국어 학습자들은 쓰기 불안을 확연하게 느끼고 있으며, 그중 3급 학습자들의 쓰기 불안이 가장 큰 반면 4급 학습자들의 쓰기 불안이 가장 낮은 걸로 나타났다. (2) 요인 분석 결과 전체 집단에서 ‘쓰기 교실 상황 또는 작문 요청시 느끼는 불안과 회피’, ‘한국어 쓰기에 대한 소극적인 태도’, ‘시간 제약과 평가에 대한 불안’, ‘작문 시 나타나는 심리적 그리고 신체적 긴장’, ‘비웃음에 대한 두려움과 혼란한 생각’과 같은 다섯 가지 쓰기 불안 구성요인이 나타났다. (3) 학습자의 TOPIK 수준에 따라 쓰기 불안의 구성요인에 차이를 보였다. 3급 학습자들은 쓰기불안의 구성요인 2, 3, 4, 5에서, 5급 학습자들은 요인 2, 3에서, 6급 학습자들은 구성요인 3에서 불안을 크게 느끼고 있었다(평균값>3.00). (4) 3급 학습자들에게서 학습 기간과 쓰기 불안 간에 양의 상관관계가 나타나 학습 기간이 길어질수록 쓰기 불안이 증가함을 보였다. The purpose of this study was twofold: To investigate whether Chinese learners of Korean would feel reading anxiety while writing Korean performance, explore what are the factors of theirs writing anxiety, and to examine whether the factors of writing anxiety would be affected by learner variables such as learning level. This study (n = 130) employed the SLWAI a survey questionnaire, and an interview method to collect data from Chinese students, who were learning Korean in Korea. Based on data analysis with SPSS 21.0, As for the results, this study figure out that (ⅰ) TOPIK 3급 participants would feel a high-level of writing anxiety while Writing in Korean, and very opposite situation was be found in the result of TOPIK 4급 participants. (ⅱ) In this study, 5 factors of writing anxiety were found such constructs as Avoidance Behavior, Evaluation Apprehension, Cognitive anxiety and so on. (ⅲ) There were significant correlations between factors of writing anxiety and learner variables such as learning level, and learning experience. and TOPIK level 3 learners feel more anxiety in all of the factors as learning period increases.

Portable Amperometric Glucose Detection based on NiS/CuS Nanorods Integrated with a Smartphone Device

Heyu Zhao,Kaige Qu,Haoyong Yin,Ling Wang,Yifan Zheng,Shumin Zhao,Shengji Wu The Korean Electrochemical Society 2023 Journal of electrochemical science and technology Vol.14 No.3

Glucose detection is particularly important for clinical diagnosis and personal prevention and control. Herein, the smartphone-based amperometric glucose sensors were constructed using the NiS/CuS nanorods (NRs) as sensing electrodes. The NiS/CuS NRs were prepared through a facile hydrothermal process accompanied by the subsequent vulcanization treatment. The morphological and structural properties of NiS/CuS NRs were characterized with SEM, EDS, XRD, and XPS. Electrochemical measurements including cyclic voltammetry, chronoamperometry, and electrochemical impedance spectroscopy display that NiS/CuS NRs can act as highly efficient electrocatalyst for glucose detection. The NiS/CuS NRs electrodes present a wide detection range of 1-8000 µM for glucose sensing with the sensitivity of 956.38 µA·mM^-1·cm^-2. The detection limit was 0.35 µM (S/N=3). When employed in smartphone-based glucose sensing device, they also display a high sensitivity of 738.09 µA·mM^-1·cm^-2 and low detection limit of 1.67 µM. Moreover, the smartphone-based glucose sensing device also presents favorable feasibility in determination of glucose in serum samples with the recoveries ranging between 99.5 and 105.8%. The results may provide a promising viewpoint to design other new portable glucose sensors.

Manganese induces neuroinflammation via NF-κB/ROS NLRP3 pathway in rat brain striatum and HAPI cells

Xinyuan Zhao,Lifeng Yin,Yifan Wu,Muxi Han,Yin Zhuang,Yewen Cong,Yiming Liu,Gang Chen,Junkang Jiang 대한독성 유전단백체 학회 2019 Molecular & cellular toxicology Vol.15 No.2

Backgrounds: Chronic exposure to excessive Mn can result in neurodegenerative symptoms, whose precise molecular mechanism remains largely unclear. Here, we measured the role and mechanism of NLRP3 in Mninduced neuroinflammation in vivo and vitro. Methods: The effects of Mn on NLRP3 activation were investigated by Westernblot, IHC, immunofluorescence analysis, as well as ELISA. We assessed NF-κB activation through measurement of phosphorylation and nuclear translocation. The mechanisms bywhich Mn induced NLRP3 activation were assessed by specific inhibitors. Results: We found that Mn exposure facilitated the activation of NLRP3 inflammasome to promote the production of IL-1β and IL-18 in dose- and time-dependent manners in HAPI cells. In addition, the NLRP3 inflammasome was also dramatically activated in microglia of rat brain striatum after Mn exposure. We also found increased ROS and NF-κB activation. Notably, the activation of NLRP3 was significantly attenuated by pretreatment with NF-κB and ROS inhibitors. Conclusion: These findings suggest that NLRP3 activation plays an important role in Mn-induced neuroinflammation, and it is associated with NF-κB and ROS.

Characterization and Expression Profile Analysis of a New cDNA Encoding Taxadiene Synthase from Taxus media

Guo Yin Kai,Ling Xia Zhao,Lei Zhang,Zhu Gang Li,Bin Hui Guo,Dong Li Zhao,Xiao Fen Sun,Zhi Qi Miao,Ke Xuan Tang 한국생활환경학회 2005 BMB Reports Vol.38 No.6