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Yu Xia,Jun-Yang Li,Yan-Kui Song,Jia-Xu Wang,Yan-Feng Han,Ke Xiao 제어·로봇·시스템학회 2023 International Journal of Control, Automation, and Vol.21 No.3
Due to the complexity of modeling and the strong transmission coupling, the rich background of rigid actuator control has not been transferred to variable stiffness actuator (VSA). Therefore, most model-based control techniques developed for VSA require feedback linearization first. Alternatively, VSA can use non-model-based control techniques such as PD control, but it does not show strong robustness under disturbances. This paper is concerned with designing a novel adaptive neural network backstepping control scheme without using feedback linearization for a special VSA with saturation inputs, output constraints, and disturbances. Firstly, for ensuring the VSA with lower tracking error and higher security, the prescribed performance-tangent barrier Lyapunov function (PP-TBLF) is introduced to handle the prescribed output performance constraints. Subsequently, the Chebyshev neural network and the Nussbaum-type function are exploited to approximate the unknown nonlinearities and unknown gains. Meanwhile, the inverse hyperbolic sine function tracking differentiator is utilized to solve the “explosion of complexity” caused by the differentiation of virtual inputs and also approximate the complex partial derivatives caused by the auxiliary control signals. Finally, the stability of the whole scheme is proved by the Lyapunov criterion. The simulation results illustrate the raised control scheme’s feasibility and show a better closed-loop behavior relative to that obtained using a classic PD controller.
Yu Xia,Laicheng Wang,Chunyan Ma,Yaoqin Gong,Yueran Zhao 연세대학교의과대학 2013 Yonsei medical journal Vol.54 No.3
Purpose: SNF2L belongs to Imitation Switch family and plays an essential role in neural tissues and gonads. In our previous studies, we have demonstrated that the basal transcription of human SNF2L gene is regulated by two cis-elements, cAMP response element (CRE)- and Sp1-binding sites. Recent studies suggested that cyclic adenosine monophosphate (cAMP) stimulation significantly up-regulated SNF2L expression in ovarian granulose cells. These data suggested that protein kinase-mediated signal pathways might also regulate SNF2L expression in neural cells. We therefore investigated the effects of agents that activate protein kinases A on SNF2L gene expression in neural cells. Materials and Methods: To increase intracellular cAMP levels, all neural cells were treated with forskolin and dbcAMP, two cAMP response activators. We exmined the effects of cAMP on the promoter activity of human SNF2L gene by luciferase reporter gene assays, and further examined the effects of cAMP on endogenous SNF2L mRNA levels by qPCR. Results: Transient expression of a luciferase fusion gene under the control of the SNF2L promoter was significantly increased by treatment of rat primary neurons with forskolin or dbcAMP, but not PC12, C6 and SH-SY5Y cells. Consistently, treatment with forskolin or dbcAMP could enhance endogenous SNF2L mRNA levels also only in rat primary neurons. Conclusion: These results suggest that the CRE consensus sequence in the SNF2L proximal promoter most likely confers constitutive activation and regulation by cAMP in neural cells.
Yuxia Zhang,Xiaoling Guo,Yuchao Cui,Chiming Guo,Liang Chen 한국식물학회 2017 Journal of Plant Biology Vol.60 No.5
In this paper, we characterized a differentiallyexpressed receptor-like cytoplasmic kinase XCRK, whichconfers resistance to bacterial leaf streak (BLS). We analyzedthe tissue expression of XCRK and showed that XCRK waswidely expressed in multiple rice (Oryza sativa) organs,including internodes, roots, leaves and flowers. In addition,the expression of XCRK was significantly induced by ABA,salt and H2O2 treatments, suggesting its function in thesepathways. The XCRK-overexpressing transgenic seedlingsexhibited higher tolerance to Xanthomonas oryzae pv.oryzicola(Xoc) compared with the wild-type seedlings. Furthermore,XCRK-overexpressing seedlings showed stronger antioxidantcapacity with reduced MDA and H2O2 content and higherantioxidant enzyme activities. It has been hypothesized thatthe enhanced Xoc tolerance was attributed to the improvedexpression of resistance-responsive factors positively regulatedby XCRK. In accordance with this, the expression of resistanceand oxidation-related genes Wrky77, Wrky13, PAL1, PR5,Fe-SOD and SodCc2 were up-regulated by the overexpressionof XCRK, which might contribute collectively to the increasedXoc tolerance. Overall, overexpression of XCRK couldenhance the antioxidant capacity and Xoc tolerance in rice.
Yuxia Liu,Zhenfa Zhang,Cuizong Zhang,Wei Huang,Caiyun Liang,Jinyun Peng 대한화학회 2016 Bulletin of the Korean Chemical Society Vol.37 No.8
Excess amount of analgesic and anti-inflammatory drug, such as indomethacin, often leads to serious gastrointestinal complications; therefore, amount of such active compound should be regulated in commercial drugs. This study proposes an efficient analytical technique to detect indomethacin selectively. We prepared and investigated electrochemical properties of a manganese dioxide-graphene nanocomposite film modified glassy carbon electrode (MnO2-Gr/GCE). The behavior of the modified electrode as electrocatalyst towards indomethacin oxidation was also examined. The cyclic voltammetric results reveal that the electrocatalytic activity for the oxidation of indomethacin can significantly be enhanced on the MnO2-Gr/GCE. Indomethacin exhibited a sensitive anodic peak at about 0.90 V at MnO2-Gr/GCE. The data obtained from differential pulse voltammetry showed that the anodic peak currents were linearly dependent on the indomethacin concentrations in the range of 1.0 × 10−7 to 2.5 × 10−5 mol/L with a detection limit of 3.2 × 10−8 mol/L (S/N = 3). Most importantly, the proposed method shows efficient and selective sensing of indomethacin in commercial pharmaceutical formulations. This is the first report of a voltammetric sensor for indomethacin using MnO2-Gr/GCE. We believe that this new method can be commercialized for routine applications in laboratories.
The Up-Regulation of miR-199b-5p in Erythroid Differentiation Is Associated with GATA-1 and NF-E2
Yuxia Li,Hua Bai,Zhongzu Zhang,Weihua li,Lei Dong,Xueju Wei,Yanni Ma,Junwu Zhang,Jia Yu,Guotao Sun,Fang Wang 한국분자세포생물학회 2014 Molecules and cells Vol.37 No.3
MicroRNAs (miRNAs) represent a class of small non-co-ding regulatory RNAs that play important roles in normal hematopoiesis, including erythropoiesis. Although studies have identified several miRNAs that regulate erythroid commitment and differentiation, we do not understand the mechanism by which the crucial erythroid transcription factors, GATA-1and NF-E2 directly regulate and control differentiation via miRNA pathways. In this study, we identified miR-199b-5p as a key regulator of human erythropoiesis, and its expression was up-regulated during the erythroid differentiation of K562 cells. Furthermore, the increase of miR-199b-5p in erythroid cells occurred in a GATA-1- and NF-E2-dependent manner during erythrocyte maturation. Both GATA-1 and NF-E2 bound upstream of the miR-199b gene locus and activated its transcription. Forced expression of miRNA-199b-5p in K562 cells affected erythroid cell proliferation and maturation. Moreover, we identified c-Kit as a direct target of miR-199b-5p in erythroid cells. Taken together, our results establish a functional link among the erythroid transcription factors GATA-1/NF-E2, miR-199b-5p and c-Kit, and provide new insights into the coupling of transcription and post-transcription regulation in erythroid differentiation.
Yuxia Duan,Pierre Servais,Marc Genest,Clemente Ibarra-Castanedo,Xavier P. V. Maldague 대한기계학회 2012 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.26 No.7
In this study, a Probability of Detection (PoD) experimental study was carried out in the framework of a Belgian-Quebec/Canada collaborative research project called ThermoPoD. Experiments were implemented on a Carbon Fiber Reinforced Plastic (CFRP) specimen with embedded material simulating delamination. For active infrared thermography, different heating sources (optical or ultrasound),heating forms (pulsed or lock-in), and data processing methods, such as Fourier Transform, Thermal Signal Reconstruction, Wavelet Transform, Differential Absolute Contrast, and Principal Component Thermography are of interest. In the present study, the effects of various data processing methods on PoD curves are compared.
The Up-Regulation of miR-199b-5p in Erythroid Differentiation Is Associated with GATA-1 and NF-E2
Li, Yuxia,Bai, Hua,Zhang, Zhongzu,li, Weihua,Dong, Lei,Wei, Xueju,Ma, Yanni,Zhang, Junwu,Yu, Jia,Sun, Guotao,Wang, Fang Korean Society for Molecular and Cellular Biology 2014 Molecules and cells Vol.37 No.3
MicroRNAs (miRNAs) represent a class of small non-coding regulatory RNAs that play important roles in normal hematopoiesis, including erythropoiesis. Although studies have identified several miRNAs that regulate erythroid commitment and differentiation, we do not understand the mechanism by which the crucial erythroid transcription factors, GATA-1and NF-E2 directly regulate and control differentiation via miRNA pathways. In this study, we identified miR-199b-5p as a key regulator of human erythropoiesis, and its expression was up-regulated during the erythroid differentiation of K562 cells. Furthermore, the increase of miR-199b-5p in erythroid cells occurred in a GATA-1- and NF-E2-dependent manner during erythrocyte maturation. Both GATA-1 and NF-E2 bound upstream of the miR-199b gene locus and activated its transcription. Forced expression of miRNA-199b-5p in K562 cells affected erythroid cell proliferation and maturation. Moreover, we identified c-Kit as a direct target of miR-199b-5p in erythroid cells. Taken together, our results establish a functional link among the erythroid transcription factors GATA-1/NF-E2, miR-199b-5p and c-Kit, and provide new insights into the coupling of transcription and post-transcription regulation in erythroid differentiation.