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Yun, J.I.,Koo, B.S.,Yun, S.W.,Lee, Chang-Kyu Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.11
Interspecies somatic cell nuclear transfer (iSCNT) is a useful method to preserve endangered species and to study the reprogramming event of a nuclear donor cell by the oocyte. Although several studies of iSCNT using murine cells and bovine oocytes have been reported, the development of murine-bovine iSCNT embryos beyond the 8-cell stage has not been successful. In this paper, we examined the developmental potential of embryos reconstructed with a murine embryonic fibroblast as the nuclear donor and a bovine oocyte as the cytoplasm recipient. The reconstructed embryos were cultured in CZB (murine medium) or CR1aa (bovine medium). In addition, for the development of a murine-bovine iSCNT blastocyst, the antioxidant ${\beta}$-mercaptoethanol (${\beta}ME$) was supplemented to CR1aa medium. Furthermore, to verify the mouse genome activation in murine-bovine iSCNT embryos, RT-PCR analysis of murine Xist was performed. The development of the murine-bovine iSCNT embryos cultured in CR1aa was significantly higher than that in CZB (p<0.05). With respect to the effect of BME on the development of the murine-bovine iSCNT blastocyst, addition of BME produced a significant increase in blastocyst development (p<0.05). Karyotype analysis confirmed that the reconstructed embryos were derived from murine cells (40XX). The Xist gene was gradually increased from the 8-cell stage to the blastocyst stage. This is the first report of blastocyst development of iSCNT embryos derived from murine somatic cells and bovine oocytes. These results demonstrate that bovine cytoplasm can support the development of later stages of a preimplantation embryo from murine-bovine iSCNT.
아동기 정서적 학대 경험이 대학생의 대인관계문제에 미치는 영향: 내면화된 수치심과 거부민감성의 매개효과
김윤지 ( Kim¸ Yun Ji ),최가희 ( Choi¸ Gahee ) 서강대학교 학생생활상담연구소 2020 人間理解 Vol.41 No.2
본 연구의 목적은 대학생의 아동기 정서적 학대 경험이 대인관계문제에 미치는 영향에서 내면화된 수치심과 거부민감성이 매개효과를 가지는지 검증하는 것이다. 연구 대상은 대한민국 전역 소재의 4년제 대학생 469명이었다. 연구 참여자는 정서적 학대 척도, 한국형 대인관계문제 단축형, 내면화된 수치심 척도, 대학생을 위한 거부민감성 질문지로 구성된 설문지에 응답하였다. 본 연구의 결과를 요약하면 첫째, 내면화된 수치심은 대학생의 아동기 정서적 학대 경험과 대인관계문제의 관계를 완전매개하는 것으로 나타났다. 둘째, 거부민감성은 대학생의 아동기 정서적 학대 경험과 대인관계문제의 관계를 부분매개하는 것으로 나타났다. 셋째, 대학생의 아동기 정서적 학대 경험과 대인관계문제의 관계를 내면화된 수치심과 거부민감성이 순차적으로 매개하는 이중 매개효과를 확인하였다. 본 연구는 대학생의 아동기 정서적 학대 경험과 대인관계문제의 관계에서 내면화된 수치심과 거부민감성이 미치는 영향의 기제를 파악하고, 이후 상담장면에서 내담자가 스스로를 수치스러워하는 경향성과 거부민감성으로 인해 경험하는 다양한 대인관계 어려움을 상담자가 이해하고 공감하도록 도움을 주었다는데 의의가 있다. This study aimed to examine the mediating effects of internalized shame and rejection sensitivity on the relationship between college students’ childhood emotional maltreatment experiences and interpersonal problems. A total of 469 college students completed questionnaires on childhood emotional maltreatment, interpersonal problems, internalized shame, and rejection sensitivity. The results indicated that the relationship between childhood emotional maltreatment and interpersonal problems is fully and sequentially mediated by internalized shame and rejection sensitivity. This study identified the roles of internalized shame and rejection sensitivity in the relationship between childhood emotional maltreatment and interpersonal problems. Moreover, it provided suggestions for effective intervention with clients who have experienced childhood emotional maltreatment.
An image thresholding method based on the target extraction
Yunjie Zhang,Yi Li,Zhijun Gao,Weina Wang 한국전산응용수학회 2008 Journal of applied mathematics & informatics Vol.26 No.3-4
In this paper an algorithm, based on extracting a certain target of an image, is proposed that is capable of performing bilevel threshold- ing of image with multimodal distribution. Each pixel in the image has a membership value which is used to denote the characteristic relationship between the pixel and its belonging region (i.e. the object or background). Using the membership values of image set, a new measurement, which simultaneously measures the measure of fuzziness and the conditional en- tropy of the image, is calculated. Then, thresholds are found by optimally minimizing calculated measurement. In addition, a fuzzy range is defined to improve the threshold values. The experimental results demonstrate that the proposed approach can select the thresholds automatically and ef- fectively extract the meaningful target from the input image. The resulting image can preserve the object region we target very well. In this paper an algorithm, based on extracting a certain target of an image, is proposed that is capable of performing bilevel threshold- ing of image with multimodal distribution. Each pixel in the image has a membership value which is used to denote the characteristic relationship between the pixel and its belonging region (i.e. the object or background). Using the membership values of image set, a new measurement, which simultaneously measures the measure of fuzziness and the conditional en- tropy of the image, is calculated. Then, thresholds are found by optimally minimizing calculated measurement. In addition, a fuzzy range is defined to improve the threshold values. The experimental results demonstrate that the proposed approach can select the thresholds automatically and ef- fectively extract the meaningful target from the input image. The resulting image can preserve the object region we target very well.
( Yunji Kim ),( Dayeon Kang ),( Ga-eun Choi ),( Sang Dae Kim ),( Sun-ja Yang ),( Hyosang Kim ),( Dalsan You ),( Choung Soo Kim ),( Nayoung Suh ) 생화학분자생물학회 2024 BMB Reports Vol.57 No.2
We investigated the therapeutic potential of bone marrow-derived mesenchymal stem cell-conditioned medium (BMSC-CM) on immortalized renal proximal tubule epithelial cells (RPTEC/ TERT1) in a fibrotic environment. To replicate the increased stiffness characteristic of kidneys in chronic kidney disease, we utilized polyacrylamide gel platforms. A stiff matrix was shown to increase α-smooth muscle actin (α-SMA) levels, indicating fibrogenic activation in RPTEC/TERT1 cells. Interestingly, treatment with BMSC-CM resulted in significant reductions in the levels of fibrotic markers (α-SMA and vimentin) and increases in the levels of the epithelial marker E-cadherin and aquaporin 7, particularly under stiff conditions. Furthermore, BMSC-CM modified microRNA (miRNA) expression and reduced oxidative stress levels in these cells. Our findings suggest that BMSC-CM can modulate cellular morphology, miRNA expression, and oxidative stress in RPTEC/TERT1 cells, highlighting its therapeutic potential in fibrotic kidney disease.
Yunji Park,Shintae Kim,Jeongjin Park,Kyungmi Kim,Woojin Jun 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
This study was aimed to investigate the effects of Perilla frutescens var. acuta water extract (PFE) on hydrogen peroxide-induced skeletal muscle cells. As a preliminary study, we confirmed the potential compounds contents and activities of antioxidant. To confirm the impact of PFE on muscle cells, we next conducted the cytotoxicity and intracellular ROS measurement in C2C12 myoblast. Results showed that safety concentration of PFE was under 200 μg/mL. Additionally, PFE might protect muscle cells on oxidative stress through the decrease of intracellular ROS level increased by hydrogen peroxide. In order to further assess the effects of PFE on myotube atrophy induced by hydrogen peroxide, we measured mRNA expression level using RT-PCR. Muscle atrophy induced by hydrogen peroxide was associated with up-regulation of atrophic factor (myostatin) and down-regulation of protein synthesis factor (mTOR). Compared to H₂O₂ groups, PFE suppressed the expression of myostain, but increased the expression of mTOR. In conclusion, PFE might have the potential to protect against myotube atrophy by regulating proteostasis and cellular redox signaling.