한약재 수침추출액의 항균효과 검색

정영건,권오진 효성여자대학교 부설 한국환경위생연구소 1993 환경위생연구 Vol.3 No.1

본 연구는 천연보존료 개발의 기초자료를 추구코저 시판되는 한약재 18종을 구입하여일반가정에서 행하는 방법대로 열탕 추출하여 공시균주인 gram 양성 3주 및 gram 음성4주에 대한 균의 성장 억제효과와 공시균주의 특성을 조사한 결과는 다음과 같다. 공시균주의 NaCl 내성은 Bacillus subtilis가 가장 약하였고 Enterobacter aerogenes가 가장강하게 나타났으며 본 균을 제외한 공시균주들은 일반적으로 성장 저해농도는 4% 이상이였다. 항생물질 내성은 모든 공시균주가 penicillin-G에서 일반적으로 내성이 가장 강했으며 streptomycin에서 내성이 가장 약하게 나타났다. Shigella sp.은 사용한 모든 항생물질에서 MIC가 100㎍/ml으로 나타나 내성이 가장 약하였고 Pseudomonas aeruginosa는400+ ㎍/ml로 내성 이 가장 강하였다. 열저항성은 Bacillus subtilis와 Enterobacter aeroge-nes가 85℃에서도 성장하여 열저항성이 가장 강했다. 18종의 한약재 중 황금, 일황연, 황백에서 Shigella sp.만이 증식이 억제되어 나타났고그 농도는 4.0%, 5.0%, 10% 이상으로 각각 나타났다. This study was set up to investigate natural preservative substan-ces among many kinds of Korean medicinal herbs. 18 kinds of Korean medicinalherbs were sampled in the traditional market of Korean medicinal herbs in Taegucity. 7 strains of bacteria were used in this experiment as test strains whichwere 3 strains of Gram positive bacteria(Bacillus subtilis, Lactobacillus caseiand Staphylococcus aureus) and Gram negative 4 strains(Enterobacter aeroge-nes, Klebsiella pneumoniae, Pseudomonas aeruginosa and Shigella sp.). Theresults summerized were as follows : 1. In susceptibilities of test strains to NaCl solutions the most sensativestrain was Bacillus subtilis white Enterobacter aerogenes was showed strongresistance to each different concentration of this solution. Almost the growthof test strains except Enterobacter aerogenes were inhibited in over 4% of thissolution. 2. Generally all of test strains were resisted to penicillin-G whereas theyall were susceptible only to streptomycin. Shigella sp. was markedly sensativeto all of antibiotics used in this experiment and MIC of this strain to them was100㎍/ml while Pseudomonas aeruginoga was strong1y tolerable to the all ofantibiotics and its MIC to them was 400+㎍/ml. 3. Bacillus subtilis and Enterobacter aerogenes were able to grow until85℃ in gradually increasing temperature. 4. Only each water extract of Scutellariae Radix, Coptidis Rhizoma andPhellodendri Cortex among 18 kinds of samples inhibited the growth of Shigellasp. with over concentrations of 4.0% , 5.0% and 10% respectively.

식육에서 분리한 Enterobacteriaceae의 분포에 대하여

정영건,권오진 효성여자대학교 부설 한국환경위생연구소 1991 환경위생연구 Vol.1 No.1

경산지역 시판식육의 위생학적인 측도를 추구코저 112시료의 식육을 채취하여 205주의 En-terobacteriaceae를 분리하고 이중 특징적인 102주를 선별 동정하여 본 실험에 이용하였으며본 분리균들의 식육에서의 분포는 다음과 같았다. (1) 24주의 Proteus 중 21주는 돈육, 3주는 우육에서 유래 되었다. (2) 8주의 Klebsiella중 7주는 돈육, 1주는 우육에서 유래 되었다. (3) 14주의 Providencia rettgeri중 11주는 돈육, 3주는 우육에서 유래 되었다. (4) 1주의 Serratia, 2주의 Morganella morganii, 2주의 Enterobacter aerogenes 및 2주의 Yersi-nia는 돈육에서 유래되었다. (5) 28주의 E.coli 중 21주는 돈육, 7주는 우육에서 유래 되었다. (6) 20주의 미동정된 균중 14주는 돈육, 6주는 우육에서 유래 되었다. On the purpose to evaluate hygienic measurement for meat on themarket near Gyongsan province, 112 of meat samples were randomly taken forour tests and 205 isolates of Enterobacteriaceae were recovered from them.Among 205 of isolates of Enterobacteriaceae typical 102 strains of them wereselected and used on this survey. Identified 102 strains of Enterobacteriaceae were originated from the meatsamples followed : (1) 24 strains of Proteus were recovered from the meat samples that 21 strainsof them were from porks and 3 strains of them were from beeves. (2) 8 strains of Klebsiella were recovered from the above same kinds of samplesthat 7 strains of them were from porks and 1 strain of them was from beef. (3) 14 strains of Providencia rettgeri were recovered from the above same kindsof samples that 11 strains of them were from porks and 3 strains of themwere from beeves. (4) 1 strain of Serratia, 2 strains of Providencia stuartii, 1 strain of Morganella morga-nii, 2 strains of Enterobacter aerogenes and 2 strains of Yersinia were recoveredfrom porks. (5) 28 strains of E. coli were recovered from the meat samples that 21 strainsof them were from porks and 7 strains of them were from beeves. (6) 20 strains Unidentified were recovered from the above same kinds of samplesthat 14 strains of them were from porks and 6 strains of them were frombeeves.

『꼭두각시놀음』과 손인형극의 비교 연구

박건웅 한국어문학회 2003 語文學 Vol.0 No.82

Der vorliegende Beitrag ist ein Versuch, das koreanische Puppenspiel "Kkoktukaksi-noleum" mit dem Handpuppenspiel zu vergleichen und ferner die heiden Worter "Kkoktukaksi" und "sanpazi" auf "kudurguk" und "cambaz" zu beziehen. Unter diesem Aspekt werden in diesem Beitrag die Fragen um dieses Puppenspiel beantwortet und versucht, seine Herkunft zu verfolgen. Erstens werden die Beziehungen des "Kkoktukaksi-noleum" mit dem Handpuppenspiel erklart. Das "Kkotukaksi-noleum" besteht aus Stockpuppen und Handpuppen. Purschke wies darauf hin, dass die Handpuppenspiele in Europa und Asien sich sicherlich aus einer gleichen Urfonn entwickelt haben, und hat viele gemeinsame Eigenschaften der Handpuppenspiele angefulut. Zu unserem Erstaunen hat auch das "Kkoktukaksi-noleum" diese Eigenschaften. Das Handpuppenspiel und das "Kkoktukaksi-noleum" haben also vieles miteinander gemein: Auffuhrungsweise: Buhne, Zungenflote, Interpret und Knuppel als Requisiten; Personenkonstellation; Handlung; Uberlieferungszeit. In der Regel wird gesagt, dass das Stockpuppenspiel in China seinen Ursprung hat und aber das Handpuppenspiel westlich von China. So gesehen erscheint das "Kkoktukaksi-noleum" als ein ZusammenscWuss dieser heiden Spielfonnen. Und daher kann das Handpuppenspiel zu einem wichtigen Ansatzpunkt fur die Untesuchung der Herkunft des "Kkoktukaksi-noleum" werden. Zweitens wird das Wort "Kkoktukaksi" in Zusammenahng mit dem Handpuppenspiel und dessen Namen erlautert. Von der Westkuste des Schwarzen Meeres bis Japan sind zwar verschiedene, jedoch homogen scheinende Namen des Puppenspiels zu finden, und beim Handpuppenspiel sind seine Namen besonders ahnlich. Unseres Erachtens haben sich diese Namen aus dem Wort "kut" mit altaischen Herkunft entwickelt, das 'Seele, Geist und Leben' bedeutet und in Korea zum scharnanischem Kult in Beziehung steht. Und dabei betrachten wir die mitteJturkischen Worter "kudurguk" und "qudurguq" als Vermittler zwischen "kut" und den heutigen verschiedenen Namen an. Die Namen der Handpuppenspiele Zentralasiens haben jeweils Endungen wie "-guk", "-gak" und "-Cak". Diese Endungen entsprechen gerade den Diminutiven des heutigen Turkischen wie "-cek", "-cuk" und "-cagiz", die "klein" und "hubsch" bedeuten. Daraus ergibt sich, dass "Kkoktukaksi" einen zwar klein, jedoch ungeheuer starken Geist verkoqJert, und dass das I-Iandp.wenSIiei in Zentralasien seinen Ursprung hat. Drittens wird "sanpazi" auf das turkische Wort "cambaz" bezogen. hn Handpuppenspiel steht eine Person, meistens ein Musiker, vor der Buhne und spricht mit den Puppen, interpretiert auch die Handlung und greift sogar in sie ein. hn "Kkoktukansi-noleum" heißt diese Person "sanpazi". In dem japanischen Puppenspiel tritt eine Person namens "sanbaso" auf. Diese beiden Worter gehen auf das turkische Wort "Dschanbaz" zuruck Dieses Wort bedeutet Spieler wie Seiltanzer und Akrobat und entspricht dem heutigen turkischen Wort "cambaz". In diesem Wort bedeutet "-baz" Spieler und "cam-" Spielplatz. Ursprunglich bedeutet "sanpazi", namlich "cambaz", Spieler und Akrobaten, also Wanderspieltruppe wie "satangpae" Die Spieler spielten auch Puppenspiel, und einer von ihnen fuhrt dabei mit den Puppenfiguren ein Gesprach. Dieser Spieler, namlich MenschenfIgur, wird besonders "camoo2" genannt, um ihn von den Puppenf1guren zu unterscheiden. Das wurde festgehalten und ist bis heute so geblieben. Dies deutet an, dass die Spieler aus Zentralasien an den Uberlieferungen des koreanischen Puppenspiels teilgenommen haben. Zum Schluss kann gesagt werden: Das koreanische Puppenspiel "Kkoktukaksi-noleum" zeigt eine enge Verbindung mit dem Handpuppenspiel, das in Zentralasien entstand und von den wandernden Berufsspielern namens "cambaz" aufgefuhrt wurde. Dieser Beitrag ist jedoch nur ein Anfang in der Untersuchungen des koreanischen Puppenspiels. Wenn wir interdisziplinar Formen und Inhalte a1Ier Puppenspiele in Europa und Asien erforscht, dann eroffnet uns das koreanische Puppenspiel "Kkoktukaksi-noleum" seine Geheinmisse.

향신료와 야채류가 세균의 성장에 미치는 영향(Ⅰ)

이건일,정영건,권오진 효성여자대학교 부설 한국환경위생연구소 1992 환경위생연구 Vol.2 No.1

본 실험은 일반가정의 식탁에서 흔히 볼 수 있는 향신료 및 야채류 14종에 대해 3주의 gram양성 및 4주의 gram 음성균의 성장에 미치는 영향을 조사하였다. 공시균의 NaCl에 대 한 내성은 Lactobacillus casei, Staphylococcus aureus, Enterobacter aeroge-nes는 7%의 NaCl에서도 성장하여 내성이 강하였고, 그외의 공시균은 5-7%의 NaCl에서 성장저해효과가 있었다. 열저항성은 75℃이상%서 공시균의 성장이 억제되어 85℃에서는 Bacillussubtilis, Enterobacter aerogenes를 제외한 공시균은 성장이 완전히 저해되었다. 항생물질의내성은 Pseudomonas aeruginosa가 본 실험에 이용된 모든 항생물질에서 MIC가 400 mg/ml로나타나 개성이 가장 강하였으며 Shigella sp.은 MIC가 100 mg/ml로 내성이 가장 약하였다.마늘에서는 Pseudomonas aeruginosa를 제외 한 모든 공시균에서 , 엽차에서는 Bacillus subtilis,Klebsiella pnewmoniae, Shigella sp.에서, 쑥에서는 Staphylococcus aureus, Enterobacter aeroge-nes, Klebsiella pneumoniae, Pseudomonas aeruginosa, Shigella sp.에서 각각 항균작용이 있었으며, 생육저해 농도는 마늘은 0.5-0.4%, 엽차는 4.0-5.0%, 쑥은 2.0-10.0%로 나타났다. This study was carried out to investigate the influence of food spi-ces and vegetables, which are commonly avaible on the dining table everyday,on bacteria growth. Total 14 kinds of food spices and vegetables were sampledin Kyungsan market in Kyungsan city. 7 strains of bacteria were used in thisexperiment as test strains which were 3 strains of Gram positive bacteria (Bacil-lus subtilis,Lactobacillus casei and Staphylococcus aureus) and Gram negative4 strains (Enterobacter aerogenes, Klebsiella pneumoniae, Pseudomonas aerugi-nosa and Shigella sp.) . The results summerrzed were as ronows: 1. The growth of all test strains except Pseudomonas aeruginosa was inhibi-ted by water extracts of raw garlic with concentrations ranging 0.5% to 4.0%. 2. Bacilus subtilis, Klebsiella pnewmoniae and Shigella sp. where not grownin tea with concentrations ranging 4.0% to 5.0%. 3. Water extracts of wormwood also interfered all strains growth exceptBacillus subtilis of which inhibitory concentrations were ranged 2.0% to 10.0%. 4. In susceptibilities of test strains to NaCl solutions the most sensitivestrain was Bacillus subtilis while Enterobacter aerogeneg was showed strongresistance to each different concentration of this solution. Almost the growthof test strains except Enterobacfer aerogenes were inhibited in over 4% of thissolution. 5. GeneraIBy all of test strains were resisted to penicillin G whereas theyall were susceptible only to streptomycin. Shigella sp. was markedly sensitiveto all of antibiotics used in this experiment and MIC of this strain to them was100 mg/ml while Pseudomonas aeruginosa was strongly tolerable to the all ofantibiotics and its MIC to them was 100+ mg/ml. 6. Bacillus subtilis and Enterobacter aerogenes were able to grow until85℃ in gradually increasing temperature.

肉鷄 및 豚肉의 處理過程 중 Salmonella屬菌의 汚染에 對한 衛生學的 硏究

鄭永健,梁成鎬 嶺南大學校 環境問題硏究所 1983 環境硏究 Vol.3 No.1

The purpospe of this study was to determine the prevalence of salmonellae from the domestic animal meats and the environments of abattoirs. The hygienic conditions of the processing plants also were evaluated in this study. One hundred numbers of broiler chicken carcasses and fifty heads of hog carcasses were used in this study and total 4.6% of salmonellae were isolated from them. From the environments of the processing plants we also isolated as 9.3% of salmonellae. For the hygienic surveys on the environments of the processing plants the methods of total bacterial cell counts and coliform groups counts were carried out in our tests. The results were as follows: 1) 7 strains of salmonellae were isolaled from 100 samples of broiler carcass skins and deteced 2 strans from 100 samples of broiler carcass leg meats. From 50 samples of hog carcasses skins 3 strains of salmonellae were isolated and also detected 2 strains from 50 samples of hog carcass meats. 2) From the environments of the processing plants 6 strains salmonellae were isolated from 75 samples of the broiler slaughter plants and detected 8 strains from 75 samples of hog abattoir. 3) The most serotypes of salmonellae were A groups(14 strains) and followed D groups(8 strains), B groups (4 strains) and C groups (2 strains) in our surveys. 4) On MIC test for the antibiotics all strains were sensitive to kanamycin even in the concentration of 0.78㎍/ml but 71.4% of the isolates were resistant to oxytetracycline and ampicillin even though in the high concentrations. 5) In the evaluation of higienic conditions the defeathering machine was contaminated as the highest numbers of bacterial cells which were 2×?? per ㎠ in the broiler slaughter plant and the hog abattoir dehairing machine was contaminated as the highest numbers of organisms, 3×?? per ㎠, where also the highest numbers of coliform groups were harboured.

뽕잎분말 첨가비율에 따른 쌀다식의 관능평가 및 영양성분 함량변화에 관한 연구

김애정,이건순,여정숙,김영호 혜전대학 식품산업연구소 2002 식품산업연구지 Vol.3 No.-

This study was carried out in order to investigate the study on the change of nutrient contents and sensory evaluation in rice Dasik according to the added level of mulberry leaf powder. The results are summarised as follows; 1. According to sensory evaluation, rice Dasik added mulberry leaf powder 2% showed higher level of appearance, color, flavor than other group. 2. With increasing mulberry leaf powder, crude protein, moisture and crude ash contents were significantly increased. The contents of crude fat was decreased with increasing mulberry leaf powder. 3. Ca, P, K and Mg contents of mulberry leaf powder 1%, 2%, 3%, 4% was higher than mulberry leaf powder free group.

화상회의시스템 구축을 위한 실시간 멀티미디어 통신시스템 설계

이상준,서영건,오해석 崇實大學校 生産技術硏究所 1995 論文集 Vol.25 No.1

We can save the moving time for taking part in meeting through the remote conferencing system. We can quickly make a decision by using video conferencing system in real-time. In this paper, we designed an prototype model of multimedia conferencing system that contained the CSCW concept. The designed system is composed of audio server, video server, and document server, and they formulate audio subsystem, video subsystem, and document subsystem respectively. The result of this paper can provide base technology of implementing conferencing system, which may be enough to develop multimedia conferencing system for using voices, still images, full motion images in communication protocol.

한약재의 식중독균에 대한 항균효과

손동화,이석일,정영건 한국위생과학회 2001 한국위생과학회지 Vol.7 No.2

선별된 한약재의 타 식중독에 대한 항균성을 paper disc젖으로 조사한 결과 황백, 황련, 정향 모두 시험한 대부분의 식중독균에 대해 강한 항균력을 가졌으며, 특히 가장 발생빈도가 높은 Escherichia coli와 Staphylococcus aureus 에 대해 높은 항균력을 보이는 것으로 조사되었다. 이상의 결과로 볼 때 황백, 황련 및 정향의 추출물은 식중돗균에 대해서 강한 항균력을 보이는 것으로 인정되어 식품보존제로서의 이용 가능한 것으로 확인되었다. Water extracts of medicinal plants were prepared to investigate antibacterial activity on pathogenic bacteria, Water extracts of phellodendron amurense Ruprcht, Coptis chinensis Franch. and syzygium aromaticum (L.) Merr. et perry were exhibited excellent growth inhibiting activities for the most of tested pathogenic bacteria. The results suggest that extracts of phellodendron amurense Ruprcht, Coptis chinensis Franch. and syzygium aromaticum (L.) Merr. et perry couid play a good role for antimimicrobial agents food.

보리등겨로 제조한 간장의 향기성분

권오준,이은정,최웅규,손동화,이석일,정연건,지원대 한국위생과학회 2002 한국위생과학회지 Vol.8 No.2

새로운 장류제품으로서 보리등겨의 이용방안을 모색하기 위하여 간장을 만들어 연구하였다. 보리로 제조한 간장의 갈색화는 점차적으로 증가 하였으며 완만한 변화를 보였다. 향기성분으로는 4-vinyl-2-methoxy-phenol, benzeneacetaldehyde, palmitic acid, 2-furancatboxaldehyde, methyl-9, 12-octadecadienoate, di-(2-ethylhexyl)phthalate, diethyl phtalate, dibytyl-1,2-benzenedicatboxylate, 5-methyl-2-furancarboxaldehyde, 3,4-dimethyl-1h-pyrazole, phenylethyl alcohol, dioctyl-hexanedioate, dimethyl-1,2-benzenedicatboxylate, benzaldehyde, methional, 2-methoxy-phenol, n-furfurylidene-3-methylbutyl amine, 1-furfuryl-2-formyl pyrrole, tetradrcanoic acid, 5-methyl-pyrimidine, 4-methyl-5-hydroxymethyl-imidazole, maltol, 5-(5-methyl-2-furanyl)methyl-2-furancarboxaldehyde 순으로 높은 함량을 차지 하였다. For investigation of new utilization as jang-products, kanjang was prepared using barely bran. This study was conducted to investigate flavor components of kanjang during fermentation time. The optical density was gradually increased. Among the flavor components identified in kanjang made with barley bran, the contents of 4-viny1-2-methoxy-phenol was the most in quantity followed by benzeneacetaldehyde, palmitic acid, 2-furancarboxaldehyde, methyl-9,12-octadecadienoate, di-(2-ethylhexyl)phthalate, diethyl phtalate, dibutyl-1,2-benzendicarboxylate, 5-methyl-2-furancarboxaldehyde, 3,4-dimethyl-1h-pyrazole, phenylethyl alcohol, dioctyl-hexanedioate, dimethyl-1,2-benzenedicarboxylate, benzaldehyde, methional, 2-methoxy-phenol, n-furfurylidene-3-methylbutyl amine, 1-furfuryl-2-formyl pyrrole, tetradecanoic acid, 5-methyl-pyrimidine, 4-methyl-5-hydroxymethyl-imidazole, maltol and 5-(5-methyl-2-furanyl)methyl-2-furancarboxaldegyde.

Lactobacillus acidophilus의 세포표층의 성상 - 세포벽외층의 규칙적 배열구조 단백질의 성상과 세포표면 소수성 -

정영건(Yung-Gun Chung),안장연(Jang-Yeon Ahn) 한국식품영양과학회 1990 한국식품영양과학회지 Vol.19 No.1

사람이나 동물의 소화관의 상재균으로서 중요한 Lactobacillus acidophilus의 여러 균주를 사용하여 세포표면의 성상을 조사하였다. 분리한 세포벽의 negative 염색한 표본을 전자현미경으로 검토한 결과, 사용한 21주중 15주는 세포벽외층에 단백질로 구성된 regular array를 생산하고 있음을 알았다. RA를 구성하는 단백질의 분자량은 41KDa~48KDa이었다. 아미노산 조성 및 Staphylococcus aureus V8 protease로 한정분해 후 및 N-chlorosuccinimide로 부분절단후의 peptide map에서 Johnson등의 분류의 subgroup A-1에 속하는 균주가 생산하는 RA단백질은 불균질하였으나, 그외 균주의 RA단백질은 같은 subgroup에 속하고 있어도 불균질하였음을 알았다.<br/> 따라서 RA생산의 유무 및 RA단백질의 분자량측정과 peptide mapping을 행하면 지금까지 행해온 DNA-hybridization과 같은 복잡한 절차를 사용하지 않아도 subgroup A-1의 L. acidophilus가 동정 가능함을 알았다. L. acidophilus의 세포표면소수성은 일반적으로 RA를 생산하지 않는 균주보다는 RA를 생산하는 균주에 높은것이 많았다. RA생산균주에 있어서는 RA단백질은 세포표면소수성과 직접관계하고 있는것은 적었다. Many strains of Lactobacillus acidophilus, which is one of the most important normal flora in the intestinal tract of human and animals, and has been used as a dietary adjunct for beneficial effect on the intestinal tract, were examined for the cell surface properties. Electron microscopy of negatively stained preparations of the isolated cell walls revealed that 15 out of 21 strains examined possessed a regularly arranged outer cell wall layer. All the L. acidophilus strains which produce regular arrays belonged to group A classified based on DNA-DNA homology by Johnson et al. (Int. J. Syst. Bacteriol. 30 ; 53~68, 1980) ; While the strains carrying no regular arrays fell under group B. The regular arrays were shown to be constructed from a single protein species with molecular weights ranging from 41 kDa to 48 kDa as analyzed by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) of the isolated cell walls.<br/> All the regularly arranged proteins isolated from the strains of subgroup A-1 described by Johnson et al. gave the same amino acid composition and peptide pattern after limited proteolysis with Staphylococcus aureus V8 protease, and were resistant to cleavage with N-chlorosuccinimide. On the other hand, the regularly arranged proteins isolated from the strains of the other subgroup A gave quite different amino acid compositions and peptide patterns one another, if the strains were in the same subgroups. These results suggest that L. acidophilus strains can divide into group A and B by examining the presence or absence of regular arrays by electron microscopy or by detecting regularly arranged proteins by SDS-PAGE of the isolated cell walls. Furthermore, the strains of subgroup A-1 can be also identified by comparing peptide patterns of the regularly arranged proteins after limited proteolysis with S. aureus V8 protease. Cell surface hydrophobicity of the regular array-producing strains of L. acidophilus is generally higher than that of the strains carring no regular arrays. However, regularly arranged proteins seemed to have no relationship to the cell surface hydrophobicity in the most regular array-producing strains.