Pulmonary inflammation caused by silica dioxide nanoparticles in mice via TXNIP/NLRP3 signaling pathway

Je‑Oh Lim,Je‑Won Ko,Tae‑Yang Jung,Woong‑Il Kim,So‑Won Pak,In‑Sik Shin,Won‑Kee Yun,Hyoung‑Chin Kim,Jeong‑Doo Heo,Jong‑Choon Kim 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.3

Background Silica dioxide nanoparticles (SiONPs) have been used for various medical applications, including therapeutics and imaging, and the use of SiONPs has increased gradually over the years. However, despite an increase in the use of SiONPs, not much is known about mechanism of action of SiONPs and their pulmonary toxicity. Objective The present study investigated the pulmonary toxicity of SiONPs and explored the underlying mechanism of action, primarily focusing on thioredoxin-interacting protein (TXNIP)/NOD-like receptor pyrin domain-containing 3 (NLRP3) in SiONPs-treated mice. We investigated the toxic effects of SiONPs in the lung of BALB/c mice administered 5, 10, and 20 mg/kg SiONPs for 3 days. Results Exposure to SiONPs markedly increased inflammatory cell counts, including those of neutrophils and macrophages, and levels of inflammatory mediators, such as interleukin (IL)-1β, IL-6, and tumor necrosis factor-α in a dose-dependent manner in the bronchoalveolar lavage fluid. Moreover, the inflammation was verified upon histopathological analysis. In addition, exposure to SiONPs increased the expression of TXNIP in a dose-dependent manner and, in turn, upregulated NLRP3 inflammasome proteins, which subsequently induced IL-1β production. Conclusion Collectively, exposure to SiONPs induced inflammation in the lungs of mice, which resulted in the activation of IL-1β production via the TXNIP-NLRP3 axis. Our results provide useful information on the pulmonary toxicity induced by SiONPs and provide insights into the underlying mechanism of action.

歌唱에 있어서 發聲과 呼吸과의 유기적 관계에 관한 硏究

尹在國 조선대학교 교육연구소 1987 교과교육연구 Vol.10 No.-

하악골 신연술 후 생성된 신생골의 조직학적 및 면역화학적 소견

윤경인,박재억 대한악안면성형재건외과학회 2001 Maxillofacial Plastic Reconstructive Surgery Vol.23 No.3

Distraction osteogenesis(DO) can be performed in the bony defect associated with trauma, anomaly, and various kinds of disease. The gap generated by DO is filled with growing callus : during the period of distraction, the osteogenesis is continued. However, there have been few reports about expression pattern of growth factors in newly formed bone during the consolidation periods. We performed DO in the mandibular defect case and studied the expressed pattern of growth factors. Its pattern was compared to that of the same patient. BMP-2 and -4 were strongly expressed in the DO site. Particularly, BMP-4 was not expressed in the normal mature bone, but expressed in new bone in DO. However, there was no difference in the FGF-7 expression between the sites. Therefore, strong expression of BMP-4 are related to new bone formation in DO and they may not be related to the normal homeostasis in human bone. Though FGF-7 is related to the growth of keratinocyte, it may have minimal role in the DO and normal mature bone.

中學校 科學科의 實驗實習 敎材의 開發硏究(Ⅲ)

尹世重,趙泰璟,吳濟直,金溫植,金黎相 공주대학교 사범대학 과학교육연구소 1983 과학교육연구 Vol.15 No.1

Teaching materials available for the effective performance of laboratory exp-erimental work in middle school were developed: Physics part ; A measuring apparatus of uniformly accelerated motion and normal force of inclined plane was developed. Chemistry part ; An imploved Eudiometer applicable in measuring reaction mole ratio and usful for a gas generating apparatus was developed. Biology part ; convenient teaching materials observing law of dominance, law of segregation, law of independence, incomplete dominance and dihybrid cross were developed. Earth science part ; A laboratory teaching materials observing formation of wave, current and graded bedding, and identification of sedimentary rocks were developed. Putting the above results of study together, the integrated suggestions were made to the effective development of science education in middle school : 1. The contents of science textbook should be simplified to the direction of the understanding of essential principles and concept. 2. The development and dirtrihbution of various supplementary teaching materials (film, T.P., cassettes film, supplementary textbook, program teaching materials individual learning materials) are required. 3. The experimental laboratory should be equipped with common instruments and equipements. 4. Efforts and time are needed to science teachers, and so remission of sundry duties and arrangement of assistant are hopefully considered. 5. Teacher's research activities should be encouraged by administrative route or relevant institute.

운동선수들의 고통 대처 반응에 관한 연구

문윤제 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.14 No.3

The purpose of this study was to investigate 1) the validity and reliability of Sports Inventory for Pain(SIP) when used Korean athletes 2) the effect according to gender, injury-potential, performance levels, grade. The participants were 485 athlete's in elementary, middle·high school and university. The SIP was developed by Meyers et al(1992) and translated into Korean by 2 Ph.D sport psychologist, head coaches. Factor analytic procedure was used to determine SIP factors. Major SIP factors included direct copy, cognitive, catastrophizing, avoidance, body awareness. MANOVAs indicated significant gender(F5,475=2.67, p<.05), injury-potential(F5,475=2.20, p<.05), performance levels(F5,475=2.65, p<.05). Findings demonstrate that the SIP can be used to differentiate between gender, injury-potential, performance, thus providing partial confirmation of construct validity of the SIP. These findings may be used as a starting point for continued research delineating and contrasting pain coping response in sport athletes.

지방행정관리에 있어서의 전략적 기획의 활용

여윤환,임영제 경희대학교 지역문제연구부 1996 지역개발연구 Vol.3 No.-

J-R 곡선에 대한 시편크기의 영향에 관한 연구

조연제,석창성,김영진,양원호,최용식 成均館大學校 科學技術硏究所 1995 論文集 Vol.46 No.1

The objective of this paper is to investigate the constraint effect on fracture resistance curves. A series of fracture toughness tests with different specimen sizes taken from nuclear piping materials(SA312 TP347 SA516 Gr. 70) were performed. Test results show that fracture toughness is increased with increase in specimen size, probably due to the relaxation crack tip constraint. Elastic-plastic finite element analyses were also performed to investigate the constraint effect. Numerical results show that the stress triaxiality at the crack tip is the governing factor for fracture toughness.

補益藥의 補血機轉에 對한 本草學的 解釋

이제현,이한구,김진숙,김연태,김정숙 대한본초학회 1997 大韓本草學會誌 Vol.12 No.2

Hematopoietic actions induced by administration of Astragali radix, Angelicae gigantis radix, Rehmanniae radix preparat and Cervi pantotrichum cornu were compared in SAM R1 and P6. We measured complete blood cells such as RBC, Hb and Hct using Coulter's method and compared the results each other in a view of traditional Korean medicine. The administration of Angelicae gigantis radix did not induce hernatopoietic actions in SAMs and also was quite different those from the other 3 herbs. Thus, the mechanisms of the hematopoietic action by Astragali radix, Angelicae gigantis radix, Rehmanniae radix preparat and Cervi pantotrichum cornu could be different each other.

산업폐기물인 폐주물사를 혼입한 콘크리트의 최적배합설계

박재선,김태경,백민경,이주형,윤경구,김남윤 강원대학교 석재복합신소재제품연구센터 1997 석재연 논문집 Vol.2 No.-

산업폐기물인 폐주물사를 재활용하여 에너지 절감과 환경오염효과를 얻을 수 있다. 반요인실험법을 사용하여 폐주물사를 잔골재에 일정비율 치환한 콘크리트의 최적배합설계 제시를 위한 예비실험에서 물-시멘트비, 폐주물사의 잔골재 치환율, 잔골재율, 슬럼프와 같은 주요변수와 변수사이의 2차상호작용을 파악하였다. 예비실험결과 폐주물사의 잔골재치환율을 70%까지 실시하였을 때 폐주물사를 혼입한 콘크리트의 강도발현에 가장 중요한 변수는 물-시멘트비로 분석되었고 폐주물사의 잔골재치환율은 거의 영향이 없는 것으로 분석되어 폐주물사를 콘크리트의 잔골재로 대체할 수 있음을 알 수 있었다. 각 변수의 2차상호작용에서는 폐주물사의 잔골재 치환율과 잔골재율의 상관관계가 콘크리트의 강도발현에 가장 큰 영향을 미치는 것으로 나타났다. 목표압축강도에 대한 최적배합조건을 폐주물사의 잔골재 치환율에 따라 물-시멘트비, 잔골재율에 대해 제시하였다. The waste foundry sand might be recycled in concrete, saving energy and protect-ing environment. An half- factorial experiments were performed with the primarary variable such as water- cement raito, waste foundry sand substitution ratio, fine agg-regate ratio and slump as a preliminary study for optimum mix design of concrete. The preliminary study show that the water- cement ratio is the most important factor to the concrete strength, followed by slump. The substitute of waste foundry sand up to 70% has little infulence, indicating that it can substitute the fine aggregate without damaging the Concrete strength. The interaction between waste foundry sand substitution ratio and fine aggregate ratio was most significant to the concrete strength among the 2-way interaction variable. Only compressive strength affect to the formation of optimum mix design area as target compressive strengh becomes bigger. Optimum mix designs of concrete incorporating waste foundry sand, satisfying the target responses, were proposed in terms of waste foundry sand substitution ratio, water cement ratio and fine aggregate ratio.

우리나라 재난의학적 처치에 대한 연구 : 1993년 3월 29일 구포 열차 전복사고를 중심으로 한 연구 ANALYSIS OF RAILROAD COLLAPSED ACCIDENT OF GUPO

백광제,홍윤식 대한응급의학회 1993 대한응급의학회지 Vol.4 No.2

Disaster in an unexpected, chaotic, horrendous catastrophes and it may be either natural or human origin. Now a days, disasters are occurring at on increasing rate due to population growth and technological advancement. Medical disaster in an incident which produces such a number of causalities that makes the routine methods for patient care ar4e inadequate. The public no linger accept these consequences as "act of God" and they demand that disaster risks to be minimized and that any impact to be management in an orderly fashion. So emergency medical personnel must strive to achieve themselves to achieve the greatest good for the greatest number of potential survivors at the disaster scene. On 28 March 1993, a train carrying 600 passengers rolled over due to railroad collapse. About 170 passengers dies(25 before extrication) and 22 severe wounded survived. Problems in the organization at the scene, triage, and the communication contributed to the confusion in the management of victims. As analysis of this accident allows several lessons to be learn which might prevent or reduce the fatalities from future disaster. Although Korean community has relied on a model of disaster response deprived from the experience of military and civil defense planners for years. A better approach to organizing medical personnel for the incident with multiple causalities, which is based on the medical response of the emergency medical services organization, will be needed in the near future.