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Reinforcement of Waterborne Polyurethane Films with Poly(acrylic acid)-Modified Palygorskite Fibers
Yu Mao,Lingli Ni,Changyou Yang,Peng Cai,Weigang Du,Xiaoyan Gao 한국섬유공학회 2020 Fibers and polymers Vol.21 No.9
Palygorskite (PAL) is a natural fibrous clay mineral which attracted tremendous attention as reinforcing agent topolymers. In this paper, a facile and environmental friendly modification process of PAL by poly acrylic acid (PAA) via insitupolymerization in PAL/water gel has been reported. The effects of PAA modified PAL (PAA-PAL) on the mechanical andthermal properties of waterborne polyurethane (WPU) nanocomposites have been investigated. Scanning electronmicroscopy (SEM) demonstrated that the dispersion of PAL has improved dramatically after PAA modification. Tensile testsshowed that PAA-PAL has a significant reinforcement effect on WPU matrix. Addition of 10 wt% PAA-PAL, the tensilestrength and the Young’s modulus of WPU composites increased 235 % and 388 %, respectively. Furthermore, the thermalstability of WPU also has been distinctly improved via addition of PAA-PAL.
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Ding, Rui,Lin, Chunnan,Wei, ShanShan,Zhang, Naichong,Tang, Liangang,Lin, Yumao,Chen, Zhijun,Xie, Teng,Chen, XiaoWei,Feng, Yu,Wu, LiHua Korean Society for Molecular and Cellular Biology 2017 Molecules and cells Vol.40 No.2
Previous studies have shown that bone marrow mesenchymal stromal cell (MSC) transplantation significantly improves the recovery of neurological function in a rat model of intracerebral hemorrhage. Potential repair mechanisms involve anti-inflammation, anti-apoptosis and angiogenesis. However, few studies have focused on the effects of MSCs on inducible nitric oxide synthase (iNOS) expression and subsequent peroxynitrite formation after hypertensive intracerebral hemorrhage (HICH). In this study, MSCs were transplanted intracerebrally into rats 6 hours after HICH. The modified neurological severity score and the modified limb placing test were used to measure behavioral outcomes. Blood-brain barrier disruption and neuronal loss were measured by zonula occludens-1 (ZO-1) and neuronal nucleus (NeuN) expression, respectively. Concomitant edema formation was evaluated by H&E staining and brain water content. The effect of MSCs treatment on neuroinflammation was analyzed by immunohistochemical analysis or polymerase chain reaction of CD68, Iba1, iNOS expression and subsequent peroxynitrite formation, and by an enzyme-linked immunosorbent assay of pro-inflammatory factors (IL-$1{\beta}$ and TNF-${\alpha}$). The MSCs-treated HICH group showed better performance on behavioral scores and lower brain water content compared to controls. Moreover, the MSC injection increased NeuN and ZO-1 expression measured by immunochemistry/immunofluorescence. Furthermore, MSCs reduced not only levels of CD68, Iba1 and pro-inflammatory factors, but it also inhibited iNOS expression and peroxynitrite formation in perihematomal regions. The results suggest that intracerebral administration of MSCs accelerates neurological function recovery in HICH rats. This may result from the ability of MSCs to suppress inflammation, at least in part, by inhibiting iNOS expression and subsequent peroxynitrite formation.
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Rui Ding,Chunnan Lin,ShanShan Wei,Naichong Zhang,Liangang Tang,Yumao Lin,Zhijun Chen,Teng Xie,XiaoWei Chen,Yu Feng,LiHua Wu 한국분자세포생물학회 2017 Molecules and cells Vol.40 No.2
Previous studies have shown that bone marrow mesenchymal stromal cell (MSC) transplantation significantly improves the recovery of neurological function in a rat model of intracere-bral hemorrhage. Potential repair mechanisms involve anti-inflammation, anti-apoptosis and angiogenesis. However, few studies have focused on the effects of MSCs on inducible nitric oxide synthase (iNOS) expression and subsequent per-oxynitrite formation after hypertensive intracerebral hemorrhage (HICH). In this study, MSCs were transplanted intracerebrally into rats 6 hours after HICH. The modified neurological severity score and the modified limb placing test were used to measure behavioral outcomes. Blood–brain barrier disruption and neuronal loss were measured by zonula occludens-1 (ZO-1) and neuronal nucleus (NeuN) expression, respectively. Concomitant edema formation was evaluated by H&E staining and brain water content. The effect of MSCs treatment on neuroinflammation was analyzed by immunohistochemical analysis or polymerase chain reaction of CD68, Iba1, iNOS expression and subsequent peroxynitrite formation, and by an enzyme-linked immunosorbent assay of pro-inflammatory factors (IL-1 and TNF-). The MSCs-treated HICH group showed better performance on behavioral scores and lower brain water content compared to controls. Moreover, the MSC injection increased NeuN and ZO-1 expression measured by immunochemistry/immunofluorescence. Furthermore, MSCs reduced not only levels of CD68, Iba1 and pro-inflammatory factors, but it also inhibited iNOS expression and peroxynitrite formation in perihematomal regions. The results suggest that intracerebral administration of MSCs accelerates neurological function recovery in HICH rats. This may result from the ability of MSCs to suppress inflammation, at least in part, by inhibiting iNOS expression and subsequent peroxynitrite formation.
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