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Yue Zhao,Meng-Fei Zhao,Mei-Lin Yang,Tian-Yu Wu,Cong-Jian Xu,Jing-Mei Wang,Chao-Jun Li,Xi Li 대한암학회 2020 Cancer Research and Treatment Vol.52 No.3
Purpose: While numerous epidemiological studies have indicated that omega-3 polyunsaturated fatty acids have anticancer properties in various cancers, the effects and mechanisms of eicosapentaenoic acid (EPA) in ovarian cancer cell growth are poorly understood. Materials and Methods: ES2 ovarian clear cell carcinoma cells and SKOV3 adenocarcinoma cells were treated with palmitic acid or EPA, followed by flow cytometry and cell counting to measure apoptosis and proliferation, respectively. A modified protein lipid overlay assay was used to further verify whether EPA was a ligand of G protein–coupled receptor 30 (GPR30) in ES2 cells. The levels of apoptosis-related genes, phosphorylated AKT, and phosphorylated ERK1/2 were detected to explore the underlying mechanism. Finally, inhibitory effect of EPA on tumor growth via GPR30 was determined in vitro and in vivo. Results: EPA suppressed ES2 ovarian clear cell carcinoma cells growth via GPR30, a novel EPA receptor, by inducing apoptosis. As a ligand of GPR30, EPA activated the GPR30-cAMP– protein kinase A signaling pathway. When GPR30 was suppressed by siRNA or its inhibitor G15, the antiproliferative action of EPA was impaired. Furthermore, EPA inhibited tumor growth by blocking the activation of AKT and ERK. In the mouse xenograft model, EPA decreased tumor volume and weight through GPR30 by blocking tumor cell proliferation. Conclusion: These results confirm that EPA is a tumor suppressor in human ovarian clear cell carcinoma cells and functions through a novel fatty acid receptor, GPR30, indicating a mechanistic linkage between omega-3 fatty acids and cancers.
Computational prediction integrating the inhibition kinetics of gallotannin on α-glucosidase
Yue, Li-Mei,Lee, Jinhyuk,Zheng, Li,Park, Yong-Doo,Ye, Zhuo-Ming,Yang, Jun-Mo Elsevier 2017 INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES Vol.103 No.-
<P><B>Abstract</B></P> <P>Due to the finding that inhibition of α-glucosidase is directly associated with treatment of several diseases, the development of a selective inhibitor for targeting α-glucosidase is important. Gallotannin (GT) is a natural ingredient that has been used as a food additive and for medicinal applications. In this study, we performed a computational docking experiment involving the pre-simulation of the binding mechanism of GT, and the effect of GT on α-glucosidase was evaluated with inhibitory kinetics based on its polyphenol properties. The computational simulations indicated that the hydroxyl groups of GT interact with several residues near the α-glucosidase active site (Met69, Tyr71, Phe177, Arg212, Asp214, Glu276, His348, Asp349, and Arg439), which could affect the catalytic function of α-glucosidase by retarding substrate access. Subsequent kinetic experiments showed that GT conspicuously inhibited α-glucosidase in a parabolic mixed-type manner (<I>IC</I> <SUB>50</SUB> =1.31±0.03μM; <I>K<SUB>i</SUB> </I> =0.41±0.032μM). Our study provides insight into the inhibition mechanism and binding manner of GT to α-glucosidase. Based on its α-glucosidase-inhibiting effect and its demonstrated safety as a naturally derived compound, GT represents a promising potential agent for treatment of α-glucosidase-associated diseases.</P>
Xin-Yue Liu,Qing-Fang Yue,Lei Chen,Xiao-Mei She,Bin Hu,Yu Hu,Ping Zou 연세대학교의과대학 2016 Yonsei medical journal Vol.57 No.2
Purpose: The objective was to determine the characteristics and prognostic factors of 86 Chinese patients with trisomy 8 aberrationsand compare the prognostic value of International Prognostic System (IPSS) and Revised IPSS (IPSS-R) in this cohort. Materials and Methods: A total of 86 cases diagnosed with primary myelodysplastic syndromes (MDS) with isolated tr8 or with tr8 and other additional cytogenetic aberrations diagnosed and treated at the Union Hospital, Tongji Medical College of Huazhong University of Science and Technology between July 2002 and March 2013 were reviewed. Results: The median survival of the entire group was 23.0 months, and acute myeloid leukemia (AML) developed in 43% (37/86) patients within the follow up time. The univariate analysis revealed that overall survival (OS) was correlated with age, thrombocytopenia,absolute neutrophil count, marrow blasts, cytogenetic status and red blood cell transfusion at diagnosis, and the multivariateanalysis revealed that age, marrow blasts, cytogenetic status and transfusion dependence were independent parameters for the OS. The cytogenetic complexity and marrow blasts had the strongest impact on the AML transformation by multivariate analysis. Comparing the two prognostic systems, both two systems could successfully discriminate risk groups for survival. IPSS-R was more refined than IPSS for predicting OS, but had no advantage in predicting the risk of AML development. Conclusion: This study confirmed the influence of clinical factors on the prognosis of 86 Chinese MDS patients with trisomy 8. In addition, IPSS-R can further refine prognostic discrimination in the IPSS risk categories.
Research on the Confucianist Political Thoughts in the 20th Century
( Wang Yue-mei ) 성균관대학교 유교문화연구소 2007 儒敎文化硏究(中文版) Vol.7 No.-
儒家政治思想是中國傳統政治思想的主流, 對于中國社會的政治、經濟、文化等諸方面都産生了極大的影響。本文論述了20世紀百年儒家政治思想的硏究歷程, 以期對未來的儒家政治思想硏究提供一個較爲系統的理論框架。 The present paper discussed the study course of the Confucian political thoughts in one hundred years of the 20th century .Maybe it can provide a systemic theory frame for the future study of the Confucian political thoughts. From 1900 to 2000,Chinese academia’s study on the Confucian political thoughts had gone through four stages as follows in this one hundred years. At the first stage(1900-1919),the newly bourgeois of reform school talked about the politics according to the lection, but the revolutionary school criticized the confucian political thoughts. At the second stage(1919-1949), the dominating social and political ideology is to criticize and denounce the Confucian. At the third stage (1949-1976), Maxism is becoming the guidance thought on the Mainland China. The Confucian political thoughts got more and more criticism and led to the Great Cultural Revolution finally. It suffered entirely denial. However, it had rised in Taiwan, HongKong and even overseas areas. At the last stage(from 1976 to this day), the Confucism is enjoying a flourish renaissance. [Article in Chinese]